Journal
NUCLEIC ACIDS RESEARCH
Volume 47, Issue 7, Pages 3568-3579Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz040
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Funding
- National Key Research and Development Program of China [2017YFD0500204, 2016YFD0500801, 2017YFC1700402]
- National Natural Science Foundation of China [81822048, 81770256, 34115109]
- Agro-scientific Research in the Public Interest [201303044]
- Natural Science Foundation of Hubei Province [2015CFA041, 2016CFA015]
- Fundamental Research Funds for the Central University [2662018PY044]
- Foundation of State Key Laboratory of Veterinary Biotechnology [SKLVBF2019103]
- National Key Research and Development Program of China
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Argonaute proteins are present and conserved in all domains of life. Recently characterized prokaryotic Argonaute proteins (pAgos) participates in host defense by DNA interference. Here, we report that the Natronobacterium gregoryi Argonaute (NgAgo) enhances gene insertions or deletions in Pasteurella multocida and Escherichia coli at efficiencies of 80-100%. Additionally, the effects are in a homologous arms-dependent but guide DNA- and potential enzyme activity-independent manner. Interestingly, such effects were also observed in other pAgos fragments including Thermus thermophilus Argonaute (TtAgo), Aquifex aeolicus Argonaute (AaAgo) and Pyrococcus furiosus Argonaute (PfAgo). The underlying mechanism of the NgAgo system is a positive selection process mainly through its PIWI-like domain interacting with recombinase A (recA) to enhance recA-mediated DNA strand exchange. Our study reveals a novel system for enhancing homologous sequence-guided gene editing in bacteria.
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