Article
Plant Sciences
Monireh Shahabi, Masoumeh Emadpour, Ahmad Moieni
Summary: A highly effective method for microtuber formation and direct shoot regeneration in the ornamental crop Cyclamen persicum was developed using in vitro leaves and tubers from different cultivars. The study investigated the effects of different levels of benzyladenine (BA) and thidiazuron (TDZ) on direct shoot regeneration and microtuber formation. The results showed that the highest number of directly regenerated shoots (27.1) was observed on leaf explants of the Pure White cultivar at 0.25 mg L-1 TDZ and 0.1 mg L-1 alpha-naphthaleneacetic acid (NAA). The highest number of proliferated microtubers (17.1) was observed from the culture of tuber cuts in media supplemented with 1.0 mg L-1 BA and 0.1 mg L-1 NAA in darkness in the Neon Pink and Pure White cultivars. The rooted shoots were successfully acclimated to the greenhouse conditions.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Biotechnology & Applied Microbiology
Grace Markin, John S. Y. Eleblu, Jacqueline N. Amissah, Samuel Reynolds, Charline Soraru, Melanie S. Craze, Emma J. Wallington, Alison R. Bentley, Eric Y. Danquah
Summary: This study successfully established that plant-derived extracts can support in vitro cowpea propagation in the absence of synthetic plant growth regulators.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Francisco Conde, Elizabeth Carmona-Martin, Jose I. Hormaza, Cesar Petri
Summary: Mango is the fifth most important fruit crop worldwide, but efficient procedures for in vitro establishment and micropropagation are lacking. The use of cotyledonary nodes as initial explants in mango proved to be effective for mass propagation. Thidiazuron induced high-frequency regeneration patterns, with the best results observed in the 'Keitt' genotype. These findings represent a qualitative advance for in vitro propagation of this recalcitrant species.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Horticulture
Najwa Amalina Haradzi, Soo Ping Khor, Sreeramanan Subramaniam, Bee Lynn Chew
Summary: This study has successfully established an efficient micropropagation protocol for Meyer lemon and documented the potential initiation of indirect somatic embryogenesis.
SCIENTIA HORTICULTURAE
(2021)
Review
Horticulture
Eveline Y. Y. Kong, Julianne Biddle, Mike Foale, Bart Panis, Stephen W. Adkins
Summary: The study focuses on the rare occurrence of shoot branching in coconuts and explores a new pathway for inducing direct shoot organogenesis through plant growth regulators in in vitro culture. It proposes a potential option for clonal propagation based on direct shoot formation, which is bolstered by the success of alternative clonal propagation protocols for oil palm and date palm.
SCIENTIA HORTICULTURAE
(2021)
Article
Forestry
Shuyu Yang, Runze Liu, Wenlong Li, Yanan Jing, Solme Pak, Chenghao Li
Summary: The present study establishes a rapid and efficient shoot regeneration method from root explants in Populus ussuriensis using plant growth regulators. The method significantly increases the number and efficiency of induced shoot buds, and the entire regeneration process is completed in a short time.
Article
Horticulture
Naseem Ahmad, Mohammad Faisal, Anees Ahmad, Abdulrahman A. Alatar, Ahmed A. Qahtan, Anshu Alok
Summary: A high-yield stable virus expression system has been developed, which is suitable for large-scale production of target proteins and preparation of antibodies.
Article
Plant Sciences
Ilham Amghar, Mohammed Ibriz, Maha Ibrahimi, Abdelaali Boudra, Fatima Gaboun, Reda Meziani, Driss Iraqi, Mouaad Amine Mazri, Ghizlane Diria, Rabha Abdelwahd
Summary: This study evaluated the effects of different growth substances on the rooting of adventitious shoots of two argan genotypes and found the optimal medium formulation to promote root development. Furthermore, high survival rates were achieved by using appropriate substrate mixtures for plantlet acclimatization, providing important insights for rapid and large-scale propagation of this endangered species.
Article
Plant Sciences
Naushad Alam, Anees Ahmad, Naseem Ahmad, Mohammad Anis
Summary: This study evaluated the effects of different polyamines as plant growth regulators on the growth and regeneration of Mucuna pruriens (L.) DC., a medicinal plant. It was found that culture medium containing high concentrations of putrescine and 6-benzyladenine promoted an increase in the number and length of shoots, while a combination of putrescine and indole-3-butyric acid promoted root formation. DNA fingerprinting was used to validate the genetic fidelity of the regenerated plants.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Multidisciplinary Sciences
Hamed Rezaei, Asghar Mirzaie-asl, Mohammad Reza Abdollahi, Masoud Tohidfar
Summary: This study investigated the effects of various disinfectants and immersion time on in vitro seed sterilization and germination of petunia. The utility of three artificial neural networks (ANNs) as modeling tools was also evaluated. The results showed that the GRNN algorithm displayed superior predictive accuracy compared to the MLP and RBF models. The non-dominated sorting genetic algorithm-II (NSGA-II) was reliable for optimizing the disinfectant concentration and immersion time.
Article
Forestry
Gessica Ferreira da Costa, Pablo Diego Silva Cabral, Fabiano Guimaraes Silva, Aurelio Rubio Neto, Maria Andreia Correa Mendonca
Summary: This study evaluated the genetic stability of H. speciosa plantlets and calluses using molecular markers, and found that the tissue culture techniques for H. speciosa micropropagation did not influence somaclonal variation, and the donor plants showed relatively low genetic variability.
Article
Plant Sciences
Shashi, Vishnu Bhat
Summary: The article introduces a rapid and effective plant regeneration method optimized through callus-mediated and direct shoot organogenesis, achieving mass multiplication and biomass production of Pennisetum pedicellatum Trin. This study provides strong support for genetic improvement of this plant species.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Alejandra Hernandez-Garcia, Enrique Ambriz-Parra, Pablo Lopez-Albarran, Jose Cruz-de Leon, Rafael Salgado-Garciglia
Summary: The endangered species Dalbergia congestiflora was successfully propagated using an in-vitro micropropagation technique, with optimized growth conditions under different hormonal treatments. The study found that a higher shoot number was obtained on MS supplemented with 1 mgl(-1) BA and 0.1 mgl(-1) NAA, and roots could be developed without the addition of IBA.
PLANT BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Mina Beigmohamadi, Movafeghi Ali, Samineh Jafari, Ali Sharafi
Summary: An efficient method for in vitro regeneration of Plumbago europaea was developed in this study, utilizing different concentrations and combinations of plant growth regulators on MS medium. TDZ was found to be the most effective cytokinin for direct shoot organogenesis, while BA and NAA were optimal for indirect organogenesis. IBA was identified as the best auxin for root growth. The study successfully established a protocol for large-scale multiplication of this important medicinal plant through micropropagation.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Horticulture
Sadia Khatoon, Wencong Liu, Chuan-bo Ding, Xinglong Liu, Yinan Zheng, Yue Zhang, Xueyan Chen, Muhammad Rauf, Fahad Alghabari, Zahid Hussain Shah
Summary: This study aimed to optimize shoot induction and proliferation protocol for different cultivars of olive using different pre-cooling treatments and 6-benzylaminopurine (BAP) concentrations. The results showed significant increases in morphological, physiological, and biochemical attributes of the olive cultivars with increasing pre-cooling treatments and BAP concentrations. The Arbosana cultivar exhibited the most dramatic changes compared to other cultivars.