Article
Engineering, Chemical
Fazia Adyani Ahmad Fuad, Nurhainis Ogu Salim
Summary: Malaria management remains challenging due to drug resistance. This study identified two potential antimalarial drugs through virtual screening analysis, which showed inhibitory effects against Pk-LDH. Further exploration and development of the compound oxalic acid may lead to a promising antimalarial drug for P. knowlesi infection.
Article
Biochemistry & Molecular Biology
Titilayo Omolara Johnson, Olugbenga Eyitayo Adeyemi, Abayomi Emmanuel Adegboyega, Segun Afolabi Olomu, Festus Enokela, Sherifat Ibrahim, Bernard Gwantu, Bukola Afolayan, Kamo Stephen, Abiodun Omokehinde Eseola, Winfried Plass, Oluyomi Stephen Adeyemi
Summary: The development of resistance to conventional antimalarial therapies has led to a need for more effective drugs. In this study, we tested several novel compounds for their potential to inhibit Plasmodium lactate dehydrogenase (pLDH). IM-3 showed the best inhibitory activity and could be considered as a potential antimalarial drug.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Yeon-Jun Kim, Jun-Seop Shin, Kang Woo Lee, Hyo-Ji Eom, Byung Gwan Jo, Jin Woo Lee, Jun Hyoung Kim, So Yeon Kim, Jung Hoon Kang, Jae-Won Choi
Summary: In this study, the expression and purification of PvLDH were successfully achieved without codon optimization. The obtained protein showed its potential tetramer structure and enzyme activity, providing valuable insights for malaria research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nurhainis Ogu Salim, Fazia Adyani Ahmad Fuad, Farahayu Khairuddin, Wan Mohd Khairulikhsan Wan Seman, Mohd Anuar Jonet
Summary: The study successfully cloned, overexpressed, and purified the Plasmodium lactate dehydrogenase, confirming the presence of active protein and providing a useful tool for further malaria research.
Article
Microbiology
Utsav Sen, Akshaykumar Nayak, Juhi Khurana, Deepu Sharma, Ashish Gupta
Summary: One major barrier in malaria prevention and control programs globally is the emergence of multidrug resistance in Plasmodium parasites. Recent research has identified PfMYST as a key protein essential for parasite survival, and the inhibitor NU9056 has shown potential in inhibiting PfMYST catalytic activity and killing P. falciparum parasites. This study provides evidence that targeting PfMYST could be a promising strategy for antimalarial therapy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Nishant Joshi, Rahul Hada, Sonal Gupta, Juveria Khan, Jeremy Dobrowolski, Pawan K. Dhar, Naresh Kumar, Shailja Singh
Summary: This study reports the highly potent anti-malarial activity of benzopyrano(4,3-b)benzopyran derivatives, which is of significant importance in treating malaria infection caused by Plasmodium falciparum. These derivatives are able to block the development of the parasite by interacting with its putative target.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Danielle Figueredo da Silva, Jessica Lima de Souza, Diego Mota da Costa Jr, David Bacelar Costa, Paulo Otavio Lourenco Moreira, Amanda Luisa da Fonseca, Fernando de Pilla Varotti, Jorddy Neves Cruz, Cleydson Breno Rodrigues dos Santos, Clayton Queiroz Alves, Franco Henrique Andrade Leite, Hugo Neves Brandao
Summary: This study aimed to evaluate the antiplasmodial activity of auraptene and poligalen against a chloroquine-resistant strain of Plasmodium falciparum. The results showed that auraptene and poligalen exhibited antiplasmodial activity against Plasmodium falciparum, with high selectivity indexes. Molecular dynamics studies demonstrated the stability of coumarins at the binding site and favorable binding energies.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Trent D. Ashton, Anna Ngo, Paola Favuzza, Hayley E. Bullen, Maria R. Gancheva, Ornella Romeo, Molly Parkyn Schneider, Nghi Nguyen, Ryan W. J. Steel, Sandra Duffy, Kym N. Lowes, Helene Jousset Sabroux, Vicky M. Avery, Justin A. Boddey, Danny W. Wilson, Alan F. Cowman, Paul R. Gilson, Brad E. Sleebs
Summary: This study focuses on refining the physicochemical properties of a new antimalarial class, with the aim to improve aqueous solubility, metabolism, and reduce adverse effects. Although the optimized compounds show potent activity against asexual parasites, they exhibit modest activity against early-stage gametocytes and no activity against pre-erythrocytic liver parasites. Surprisingly, the refined physicochemical properties did not lead to improved oral efficacy in a mouse model of malaria compared to previous studies.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Panasa Mahesh, Parameswari Akshinthala, Naresh Kumar Katari, Lavleen Kumar Gupta, Dikshita Panwar, Manoj Kumar Sharma, Sreekantha Babu Jonnalagadda, Rambabu Gundla
Summary: In this study, two new series of 3,5-dimethyl-1H-pyrazole-4-sulfonamide and 1,3,5-trimethyl-1H-pyrazole-4-sulfonamide derivatives were designed and synthesized. These derivatives were characterized by various analytical techniques and their biological evaluation data were presented, paving the way for the development of new pyrazole-4-sulfonamide derivatives.
Article
Biochemistry & Molecular Biology
Francis Kayamba, Mbuso Faya, Ofentse Jacob Pooe, Babita Kushwaha, Narva Deshwar Kushwaha, Vincent A. Obakachi, Vincent O. Nyamori, Rajshekhar Karpoormath
Summary: This review highlights the importance of parasitic lactate dehydrogenases (LDH) and malate dehydrogenases (MDH) as potential therapeutic targets for the development of novel antiparasitic agents. These enzymes play a critical role in the progression and development of parasitic diseases, making them essential for the future development of effective treatments.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Medicine, General & Internal
Murat Yilmaz, Beliz Oztok Tekten
Summary: This study aimed to determine the potential role of postictal serum LDH activities in differentiating epileptic seizures from non-epileptic seizures. The results showed significantly higher LDH activities in patients with epileptic seizures compared to those with non-epileptic seizures, suggesting the potential use of LDH activities as a diagnostic tool in distinguishing between seizure types.
Review
Chemistry, Medicinal
Cornelius Nerlich, Nathan H. Epalle, Philip Seick, Eric Beitz
Summary: Malaria parasites in the blood stage rely on anaerobic glycolysis in erythrocytes for energy, utilizing glucose from the host and producing lactate and protons as waste. A missing Plasmodium lactate/H+ cotransporter was identified as a member of the FNT family, and specific FNT-inhibitors were discovered through screening of antimalarial compounds.
Article
Immunology
Angelica F. Tan, Sitti Saimah Binti Sakam, Giri S. Rajahram, Timothy William, Mohammad Faruq Abd Rachman Isnadi, Sylvia Daim, Bridget E. Barber, Steven Kho, Colin J. Sutherland, Nicholas M. Anstey, Seda Yerlikaya, Donelly A. van Schalkwyk, Matthew J. Grigg
Summary: The study evaluated ten rapid diagnostic tests for detection of P. knowlesi, with some tests showing sufficient performance for detecting major human malaria species (including P. knowlesi) in co-endemic areas, particularly for higher parasite counts. However, these tests cannot reliably differentiate non-falciparum malaria.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Atilio Reyes Romero, Serjey Lunev, Grzegorz M. Popowicz, Vito Calderone, Matteo Gentili, Michael Sattler, Jacek Plewka, Michal Taube, Maciej Kozak, Tad A. Holak, Alexander S. S. Domling, Matthew R. Groves
Summary: Romero et al. conducted NMR-based screening of 1500 fragments to identify those binding at the oligomeric interface of malate dehydrogenase (MDH). Their study indicates an allosteric mechanism affecting enzymatic activity, paving the way for development of more selective molecules and serving as a starting point for future specific MDH inhibitor development.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Medicinal
Godwin Akpeko Dziwornu, Dina Coertzen, Meta Leshabane, Constance M. Korkor, Cleavon K. Cloete, Mathew Njoroge, Liezl Gibhard, Nina Lawrence, Janette Reader, Mariette van der Watt, Sergio Wittlin, Lyn-Marie Birkholtz, Kelly Chibale
Summary: A novel series of antimalarial benzimidazole derivatives incorporating phenolic Mannich base side chains at the C2 position possess high activities against both asexual blood and sexual stages. 1-benzylbenzimidazole analogues demonstrate microtubule inhibitory activity and low cytotoxicity to mammalian cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Infectious Diseases
Lauren E. Gibson, Christine F. Markwalter, Danielle W. Kimmel, Lwiindi Mudenda, Saidon Mbambara, Philip E. Thuma, David W. Wright
Article
Public, Environmental & Occupational Health
Christine F. Markwalter, Lauren E. Gibson, Lwiindi Mudenda, Danielle W. Kimmel, Saidon Mbambara, Philip E. Thuma, David W. Wright
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2018)
Article
Infectious Diseases
Christine F. Markwalter, Lwiindi Mudenda, Mindy Leelawong, Danielle W. Kimmel, Armin Nourani, Saidon Mbambara, Philip E. Thuma, David W. Wright
Review
Chemistry, Multidisciplinary
Christine F. Markwalter, Andrew G. Kantor, Carson P. Moore, Kelly A. Richardson, David W. Wright
Article
Infectious Diseases
Abena S. Amoah, Pytsje T. Hoekstra, Miriam Casacuberta-Partal, Luc E. Coffeng, Paul L. A. M. Corstjens, Beatrice Greco, Lisette van Lieshout, Mark D. Lim, Christine F. Markwalter, Maurice R. Odiere, Jutta Reinhard-Rupp, Meta Roestenberg, Russell Stothard, Louis-Albert Tchuem Tchuente, Sake J. de Vlas, Govert J. van Dam
LANCET INFECTIOUS DISEASES
(2020)
Article
Biochemical Research Methods
Andrew G. Kantor, Christine F. Markwalter, Armin Nourani, David W. Wright
Summary: Rapid diagnostic tests (RDTs) are crucial for malaria elimination campaigns, but their sensitivity is limited. AnDREW, an Antibody-free Dual-biomarker Rapid Enrichment Workflow, purifies HRP2 and PLDH from larger blood samples, significantly improving detection sensitivity. By utilizing aptamers and optimizing capture and elution processes, AnDREW enhances RDTs by eluting both biomarkers without the need for complexed antibodies.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Public, Environmental & Occupational Health
Christine F. Markwalter, Billy Ngasala, Tonelia Mowatt, Christopher Basham, Zackary Park, Mwajabu Loya, Meredith Muller, Christopher Plowe, Myaing Nyunt, Jessica T. Lin
Summary: Ultrasensitive PCR has shown a higher burden of asymptomatic malaria infections compared to RDTs or standard PCR in low-transmission endemic areas, with potential implications for the infectious reservoir. A study in Tanzania found good correlation between DNA-based qPCR and RNA-based usPCR in detecting Plasmodium falciparum parasite densities, indicating an incremental increase in low-density infections. The extent to which these low-density infections contribute to the infectious reservoir in different malaria transmission settings remains unclear.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Multidisciplinary Sciences
Sara C. Johnston, Keersten M. Ricks, Alexandra Jay, Jo Lynne Raymond, Franco Rossi, Xiankun Zeng, Jennifer Scruggs, David Dyer, Ondraya Frick, Jeffrey W. Koehler, Paul A. Kuehnert, Tamara L. Clements, Charles J. Shoemaker, Susan R. Coyne, Korey L. Delp, Joshua Moore, Kerry Berrier, Heather Esham, Joshua Shamblin, Willie Sifford, Jimmy Fiallos, Leslie Klosterman, Stephen Stevens, Lauren White, Philip Bowling, Terrence Garcia, Christopher Jensen, Jeanean Ghering, David Nyakiti, Stephanie Bellanca, Brian Kearney, Wendy Giles, Nazira Alli, Fabian Paz, Kristen Akers, Denise Danner, James Barth, Joshua A. Johnson, Matthew Durant, Ruth Kim, Jay W. Hooper, Jeffrey M. Smith, Jeffrey R. Kugelman, Brett F. Beitzel, Kathleen M. Gibson, Margaret L. M. Pitt, Timothy D. Minogue, Aysegul Nalca
Summary: Airborne transmission of SARS-CoV-2 is found to be a prevalent route of human exposure. In a study comparing African green monkeys, rhesus macaques, and cynomolgus macaques exposed to the virus, respiratory abnormalities and viral shedding were observed in all animals. Fever was noted in cynomolgus macaques, while African green monkeys and rhesus macaques experienced thrombocytopenia. The findings suggest that macaques, in addition to African green monkeys, can serve as successful natural transmission models for evaluating medical countermeasures.
Article
Infectious Diseases
Christine F. Markwalter, Myat Htut Nyunt, Zay Yar Han, Ricardo Henao, Aarti Jain, Omid Taghavian, Philip L. Felgner, Kay Thwe Han, Myaing M. Nyunt, Christopher Plowe
Summary: This study demonstrated that malaria-specific antibody responses can be effectively analyzed from dried blood spots (DBS), providing a potential alternative to serum for serological studies in remote regions. Despite some loss of antibody activity in DBS, trends in antigen responses were consistent between DBS and serum samples. Serological testing using DBS could be useful for identifying exposure to asymptomatic, low-density malaria infections in populations where serum collection and storage are difficult.
Article
Virology
Susan L. Uprichard, Amornrat O'Brien, Monika Evdokimova, Cynthia L. Rowe, Cara Joyce, Matthew Hackbart, Yazmin E. Cruz-Pulido, Courtney A. Cohen, Michelle L. Rock, John M. Dye, Paul Kuehnert, Keersten M. Ricks, Marybeth Casper, Lori Linhart, Katrina Anderson, Laura Kirk, Jack A. Maggiore, Andrew S. Herbert, Nina M. Clark, Gail E. Reid, Susan C. Baker
Summary: This study describes the antibody responses to mRNA vaccines in a cohort of 102 subjects, showing that a single exposure to antigen induces a variable antibody response affected by demographic factors and co-morbidities. The study also finds that COVID-19-experienced individuals achieve higher antibody levels and neutralization activity. Furthermore, COVID-19-naive individuals tend to have a stronger response to the Moderna vaccine compared to the Pfizer-BioNTech vaccine.
Article
Virology
Joseph W. Golden, Collin J. Fitzpatrick, John J. Suschak, Tamara L. Clements, Keersten M. Ricks, Mariano Sanchez -Lockhart, Aura R. Garrison
Summary: Crimean-Congo hemorrhagic fever (CCHF) is a global health threat, and a clear definition of the immune correlates of protection in CCHFV vaccines is lacking. This study found that CD8+ T cell responses are necessary and sufficient to promote protection in mice vaccinated with a DNA-based vaccine targeting CCHFV. These findings provide important insights for the development of CCHFV vaccines.
Article
Infectious Diseases
Paul A. Kuehnert, Christopher P. Stefan, Catherine V. Badger, Keersten M. Ricks
Summary: This review focuses on the complex relationship between virus, vector, and host, emphasizing the need for increased biosurveillance programs for CCHFV. The review highlights gaps in recent literature on CCHFV surveillance efforts and underscores the importance of a One-Health approach in detecting and monitoring the virus.
CURRENT TROPICAL MEDICINE REPORTS
(2021)
Article
Chemistry, Analytical
Christine F. Markwalter, Paul L. A. M. Corstjens, Claire M. Mammoser, Garrett Camps, Govert J. van Dam, David W. Wright
Article
Biochemical Research Methods
C. F. Markwalter, I. K. Jang, R. A. Burton, G. J. Domingo, D. W. Wright
ANALYTICAL BIOCHEMISTRY
(2017)
Article
Biochemical Research Methods
Westley S. Bauer, Kelly A. Richardson, Nicholas M. Adams, Keersten M. Ricks, David J. Gasperino, Simon J. Ghionea, Mathew Rosen, Kevin P. Nichols, Bernhard H. Weigl, Frederick R. Haselton, David W. Wright
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
