Article
Biotechnology & Applied Microbiology
Fei Xiong, Xiangyun Cheng, Chao Zhang, Roland Manfred Klar, Tao He
Summary: This study investigated the optimal schemes for reference gene identification in bone and cartilage bioengineering experiments. The results showed that different selection schemes had a significant effect on the eventual normalization of target genes, highlighting the importance of careful consideration in selecting the appropriate reference genes for RT-qPCR assays.
Article
Environmental Sciences
Ju Mu, Yu Wang, Miao Wang, Dayi Zhang, Mingying Liu
Summary: This study constructed a mouse model exposed to poly-propylene microplastics (PP-MPs) to evaluate the bioaccumulation of PP-MPs in the kidney and explore potential reference genes using RT-qPCR. The stability and reliability of 19 candidate reference genes were assessed using four algorithms, and the top five most stable genes were determined (Cox4i1, Rps3, Rps9, Rps18, and Gapdh). Three genes associated with different biochemical processes were chosen to verify the identified reference genes, showing different expression profiles and emphasizing the importance of selecting reliable reference genes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Nina Franko, Lucija Ana Vrscaj, Taja Zore, Barbara Ostanek, Janja Marc, Jasna Lojk
Summary: RT-qPCR is the gold standard method for measuring gene expression, and selection of appropriate reference genes for normalization is crucial. This study identified the most stable reference genes during osteogenic differentiation of human osteosarcoma cell lines using statistical algorithms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sarah Arrowsmith
Summary: This study evaluated the stability of reference genes in pregnant human myometrium and found CYC1, YWHAZ, and ATP5B to be the most stable genes. On the other hand, ACTB, 18s, and B2M were found to be less stable and too variable for accurate normalization.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Plant Sciences
Maria Noelia Ulrich, Esteban Muniz-Padilla, Alejandra Corach, Esteban Hopp, Daniela Tosto
Summary: This study evaluated the stability of different reference genes of glyphosate-resistant S. halepense, finding that PP2A and ARI8 were the most stable genes. The results provide valuable information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Article
Plant Sciences
Leandro Francisco de Oliveira, Amanda Rusiska Piovezani, Dimitre A. Ivanov, Leonardo Yoshida, Eny Iochevet Segal Floh, Massuo Jorge Kato
Summary: This study identified stable reference genes for gene expression analysis in four Piper species and provided a list of recommended gene pairs for each species. These findings will facilitate further transcriptomic and functional gene research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Chaofan Jiang, Lin Zhou, Qingchen Zhao, Mengke Wang, Sirui Shen, Te Zhao, Kaidi Cui, Leiming He
Summary: This study validated the expression stability of seven candidate reference genes in Sclerotium rolfsii under different conditions and recommended suitable reference gene combinations for RT-qPCR normalization. The results showed that using one or two reference genes to normalize the expression of target genes in S. rolfsii did not significantly differ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Jianfei Chen, Ziwei Chen, Jilong Zhang, Wuchao Zhuang, Xiaotong Zheng
Summary: This study utilized different algorithms to evaluate the stability of internal reference genes in the gastrointestinal tract of chickens. The most suitable reference genes for each type of GI tissue were identified. Commonly used internal reference genes exhibited poor stability and are not recommended for future gene expression studies in the GI region.
Article
Plant Sciences
Qizhang Wang, Chunqian Guo, Shipeng Yang, Qiwen Zhong, Jie Tian
Summary: The aim of this study was to identify suitable reference genes for gene expression analysis during different growth conditions in garlic. Nine candidate reference genes were selected using garlic transcriptome sequence data and their expression levels were evaluated in specific tissues under drought and cold stress. Several statistical methods were used to assess the stability of the reference gene expression levels. The most stable reference genes were identified, and their reliability was confirmed by evaluating the expression of a stress-responsive gene. This study provides a theoretical reference for gene expression analysis in garlic under stress conditions.
Article
Green & Sustainable Science & Technology
Jing Xu, Jing Li, Tianpeng Gao
Summary: This study aims to select suitable reference genes for gene expression quantification in E. crassipes and analyze the expression stability of candidate reference genes in different tissue samples, hormone-treated samples, phosphorus-treated samples, and mixed samples. The results suggest that different reference genes should be selected according to the specific experimental setup.
Article
Genetics & Heredity
Dongxiao Ma, Qin Zhang, Jintao Zhou, Yu Lu, Xiaomeng Duan, Chengzhong He, Jinde Yu
Summary: In this study, the expression stability of 10 candidate reference genes was analyzed in Toxicodendron succedaneum using RT-qPCR. The results showed that TsRG03 and ACT were recommended as suitable reference genes for all samples. These findings provide alternative reference genes for gene function studies, correction and normalization of target gene expression, and directed molecular breeding in T. succedaneum.
Article
Plant Sciences
Francisca Valenzuela, Vivian D'Afonseca, Ricardo Hernandez, Aleydis Gomez, Ariel D. Arencibia
Summary: This study validates the reference genes in blueberry plants cultured in vitro on a colchicine-supplemented medium for the first time. The results show that colchicine treatments can cause genomic changes in blueberry plants and affect gene expression at the molecular level. The study also identifies potential reference genes for gene expression assays in Vaccinium.
Article
Agriculture, Multidisciplinary
Yao-ke Duan, Rong Han, Yan Su, Ai-ying Wang, Shuang Li, Hao Sun, Hai-jun Gong
Summary: Tomato is a model plant for fruit development and stress response research, with gene expression analysis frequently conducted through techniques like qPCR. This study identified new reference genes in tomato by mining transcriptome sequencing data, evaluating their stability under different treatments. The results showed that the stability of reference genes varied under different conditions, with most genes recommended as preferred reference genes.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Virology
Cristina Ceriani, Gabrielle S. Streeter, Kena J. Lemu, Katherine S. James, Simon Ghofrani, Brigitte Allard, Bonnie E. Shook-Sa, David M. Margolis, Nancie M. Archin
Summary: The study evaluated the stability of reference genes (RGs) after latency-reversing agent (LRA) exposure and found that TBP, UBE2D2, and RPL27A were the most stable RGs, while GAPDH showed the most variability. Using unstable RGs significantly impacted the accuracy of host gene and HIV gene expression data.
JOURNAL OF VIROLOGY
(2021)
Article
Genetics & Heredity
Shen Zhu, Yongjun Liu, Mingtao Liao, Yang Yang, Yu Bai, Na Li, Sheng Li, Yunxia Luan, Nan Chen
Summary: This study evaluated the expression variation of common insect reference genes in German cockroaches and American cockroaches through multiple algorithms and programs, identified the optimal reference genes for specific conditions for qPCR normalization, and recommended different combinations of reference genes for accurate gene expression profiling in these two important cockroach species.
Review
Endocrinology & Metabolism
Roberto Ravazzolo, Serena Cappato, Renata Bocciardi
Article
Immunology
Mario Tirone, Anna Giovenzana, Arianna Vallone, Paola Zordan, Martina Sormani, Pier Andrea Nicolosi, Raffaela Meneveri, Carmen Rosaria Gigliotti, Antonello E. Spinelli, Renata Bocciardi, Roberto Ravazzolo, Ingrid Cifola, Silvia Brunelli
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Paola Monti, Yari Ciribilli, Giorgia Foggetti, Paola Menichini, Alessandra Bisio, Serena Cappato, Alberto Inga, Maria Teresa Divizia, Margherita Lerone, Renata Bocciardi, Gilberto Fronza
BIOSCIENCE REPORTS
(2019)
Letter
Genetics & Heredity
Laura Guazzarotti, Ilaria Sani, Sabrina Giglio, Francesco Brunello, Giorgio Perilongo, Renata Bocciardi
Review
Biochemistry & Molecular Biology
Serena Cappato, Riccardo Gamberale, Renata Bocciardi, Silvia Brunelli
Review
Biochemistry & Molecular Biology
Roberto Ravazzolo, Renata Bocciardi
Summary: This article summarizes the basic research on the genetic mutation responsible for FOP, including advancements in related fields and potential therapeutic approaches. It also explores the reasons why the single mutation R206H in the ACVR1 gene is highly prevalent in FOP patients, as well as factors that may modulate FOP expression.
Article
Genetics & Heredity
Serena Cappato, Rasa Traberg, Jolita Gintautiene, Federico Zara, Renata Bocciardi
Summary: Fibrodysplasia Ossificans Progressiva (FOP) is a rare autosomal dominant disease characterized by congenital malformation of the great toes and progressive heterotopic ossification of soft tissues. A novel variant in the ACVR1 gene was identified in a 3-year-old child with early and highly suggestive clinical features of FOP, despite the absence of the recurrent p.R206H substitution commonly found in FOP patients.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Serena Cappato, Aldamaria Puliti, Maria Teresa Divizia, Margherita Lerone, Federico Zara, Renata Bocciardi
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Medical Laboratory Technology
Genny Del Zotto, Francesca Antonini, Irma Azzari, Claudio Ortolani, Gino Tripodi, Francesca Giacopelli, Serena Cappato, Lorenzo Moretta, Roberto Ravazzolo, Renata Bocciardi
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2018)
Correction
Rheumatology
Ilham Ratbi, Renata Bocciardi, Asmaa Regragui, Roberto Ravazzolo, Abdelaziz Sefiani
CLINICAL RHEUMATOLOGY
(2018)