Article
Cell Biology
Krishna K. Sarangapani, Lori B. Koch, Christian R. Nelson, Charles L. Asbury, Sue Biggins
Summary: Sarangapani, Koch, Nelson et al. demonstrate that phosphorylation by the evolutionarily conserved Mps1 kinase weakens kinetochore-microtubule attachments, aiding in mitotic error correction and ensuring accurate chromosome segregation.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.
Article
Biochemistry & Molecular Biology
Daniel Hayward, Emile Roberts, Ulrike Gruneberg
Summary: In eukaryotes, the spindle assembly checkpoint is important for maintaining genome stability during mitosis. MPS1, together with Aurora B and PP2A-B56, plays a critical role in error correction and checkpoint signaling. MPS1 localizes to end-on attached kinetochores and promotes microtubule release, contributing to the resolution of incorrect microtubule-kinetochore attachments and the initiation of spindle checkpoint signaling.
Article
Biochemistry & Molecular Biology
Vilte Stonyte, Maria Mastrangelopoulou, Romy Timmer, Lilian Lindbergsengen, Marina Vietri, Coen Campsteijn, Beata Grallert
Summary: GCN2/eIF2 alpha K4 serves as an eIF2 alpha kinase in stress response and unexpectedly acts as a regulator of mitosis in unstressed cells. Its function in mitosis is not related to translation reprogramming, but through the regulation of PP1 alpha and gamma, which were previously unidentified substrates. Inhibition of GCN2 or Aurora A leads to mitotic errors and cell death, suggesting the potential therapeutic utility of GCN2 inhibitors. These findings provide insights into the timely regulation of phosphorylation events during early mitosis.
Review
Cell Biology
Yuqing Zhang, Chunlin Song, Lei Wang, Hongfei Jiang, Yujing Zhai, Ying Wang, Jing Fang, Gang Zhang
Summary: This review summarizes the research progress on the role of Bub1 in mitosis over the past three decades. The studies have revealed that Bub1 plays important roles in checkpoint regulation and chromosome alignment. The essentiality of Bub1 in higher eukaryotic cells is discussed, along with some unresolved questions raised for future study.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qingfeng Wu, Shiren Sun, Lei Wei, Minna Liu, Hao Liu, Ting Liu, Ying Zhou, Qing Jia, Di Wang, Zhen Yang, Menglu Duan, Xiaoxia Yang, Peisong Gao, Xiaoxuan Ning
Summary: This study reveals a significant association between Twist1 expression in macrophages and the severity of renal fibrosis in IgA nephropathy patients and mice with unilateral ureteral obstruction. Twist1 ablation in macrophages alleviates renal tubular injury and fibrosis, accompanied by reduced macrophage infiltration and M2 polarization in the kidney. Twist1 downregulation inhibits macrophage chemotaxis and migration, at least partially through the CCL2/CCR2 axis. This study highlights the regulation of macrophage plasticity through Twist1/galectin-3 signaling and its potential as a new strategy for delaying kidney fibrosis in chronic kidney disease patients.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Han Ling, Chen-Hui Cao, Kai Han, Yong-Rui Lv, Xiao-Dan Ma, Jing-Hua Cao, Jie-Wei Chen, Si Li, Jin-Long Lin, Yu-Jing Fang, Zhi-Zhong Pan, Dan Xie, Feng-Wei Wang
Summary: CEP63 overexpression in colorectal cancer plays a crucial role in promoting cell proliferation and tumor growth through the regulation of RBPs/RNA axis.
Article
Genetics & Heredity
Spencer G. Gordon, Lisa E. Kursel, Kewei Xu, Ofer Rog
Summary: The study focused on a protein SYP-1 in the Synaptonemal Complex (SC) in nematodes and identified mutations that have distinct effects on chromosome pairing and information exchange during meiosis. The findings provide new insights into the structure/function relationships within the SC and propose a model linking molecular events in the SC zipper to chromosome-wide regulation. The mutations perturb both chromosome alignment and the regulation of genetic exchanges, suggesting a model where SC brings homologous chromosomes together through two activities: obligate zipping and extending pairing interactions.
Article
Biochemistry & Molecular Biology
Babhrubahan Roy, Simon J. Y. Han, Adrienne N. Fontan, Soubhagyalaxmi Jema, Ajit P. Joglekar
Summary: Accurate chromosome segregation during cell division requires proper attachment between the spindle apparatus and the chromosomes. This is ensured by the combined activity of the spindle assembly checkpoint (SAC) and an error correction mechanism. Research has shown that Aurora B kinase directly promotes the production of the mitotic checkpoint complex (MCC) and works downstream of Mps1 in promoting SAC signaling.
Article
Oncology
Ming Meng, Liting Yang, Hongshu Zhou, Quan Cheng, Renjun Peng, Zeyu Wang, Xisong Liang, Jie Wen, Jilin Nie, Zhongliang Hu, Liyang Zhang, Zhixiong Liu
Summary: LINC00978 is highly expressed in high-grade gliomas and its down-regulation inhibits cell proliferation, invasion, migration, and induces apoptosis in glioblastoma cells. LINC00978 regulates the expression of AKR1B1, which mediates the cytotoxicity of 2-deoxyglucose, and promotes the sensitivity of glioblastoma cells to 2DG.
Article
Oncology
Patrick Schoeffski, Ahmad Awada, Anne-Marie de la Bigne, Zakia Felloussi, Mike Burbridge, Frederique Cantero, Riccardo Colombo, Sara Maruzzelli, Katia Ammattatelli, Maja de Jonge, Philippe Aftimos, Herlinde Dumez, Stefan Sleijfer
Summary: S81694 can be safely administered as a single agent in adults with solid tumors at a dose of up to 135 mg/m(2)/week on days 1,8,15 of a 28-day cycle, without reaching the MTD.
EUROPEAN JOURNAL OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Xiao Guo, Ivan Ramirez, Yenni A. Garcia, Erick F. Velasquez, Ankur A. Gholkar, Whitaker Cohn, Julian P. Whitelegge, Bobby Tofig, Robert Damoiseaux, Jorge Z. Torres
Summary: This study identifies the importance of dual-specificity phosphatase 7 (DUSP7) in regulating chromosome alignment during mitosis by regulating the levels of active phospho-ERK2. Overexpression of DUSP7 or knockdown of ERK2 results in chromosome misalignment or defective chromosome congression during mitosis, supporting a model where MEK and DUSP7 cooperatively regulate cell division.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Qiang Fang, Xue-Lin Chen, Lei Zhang, Ya-Bin Li, Tian-Zeng Sun, Chen-Xin Yang, Jian-Feng Chang, Xiao-Mei Yang, Feng Sun
Summary: MPS1 is crucial for spermatogenesis, and its absence may lead to cell loss and meiosis blockage during spermatogenesis. MPS1 plays a key role in the formation of sperms and fertility.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Jodi D. Weiss, Shelby L. McVey, Sarah E. Stinebaugh, Caroline F. Sullivan, R. Kelly Dawe, Natalie J. Nannas
Summary: The assembly and disassembly of spindles in maize meiosis exhibit variability but follow a predictable series of events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Miram Meziane, Rachel Genthial, Jackie Vogel
Summary: Spindle positioning is crucial for successful cell divisions, mediated by the protein Kar9 in budding yeast. However, asymmetry of Kar9 may not necessarily correlate with stable spindle alignment, with alignment potentially being more related to Kar9 residence in the bud.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Hui Li, Xiaobao Ying, Lina Shang, Bryce Redfern, Nicholas Kypraios, Xuejun Xie, FeiFei Xu, Shaopeng Wang, Jinghua Zhang, Hongju Jian, Hongtao Yu, Dianqiu Lv
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Environmental Sciences
Ana He, Xiaoping Li, Yuwei Ai, Xiaolong Li, Xiaoyun Li, Yuchao Zhang, Yu Gao, Bin Liu, Xu Zhang, Meng Zhang, Liyuan Peng, Ming Zhou, Hongtao Yu
ENVIRONMENT INTERNATIONAL
(2020)
Review
Biochemistry & Molecular Biology
Catherine Hall, Hongtao Yu, Eunhee Choi
EXPERIMENTAL AND MOLECULAR MEDICINE
(2020)
Correction
Multidisciplinary Sciences
Jinseo Park, Ziao Fu, Aurel Frangaj, Jonathan Liu, Lidia Mosyak, Tong Shen, Vesna N. Slavkovich, Kimberly M. Ray, Jaume Taura, Baohua Cao, Yong Geng, Hao Zuo, Yongjun Kou, Robert Grassucci, Shaoxia Chen, Zheng Liu, Xin Lin, Justin P. Williams, William J. Rice, Edward T. Eng, Rick K. Huang, Rajesh K. Soni, Brian Kloss, Zhiheng Yu, Jonathan A. Javitch, Wayne A. Hendrickson, Paul A. Slesinger, Matthias Quick, Joseph Graziano, Hongtao Yu, Oliver Fiehn, Oliver B. Clarke, Joachim Frank, Qing R. Fan
Article
Chemistry, Multidisciplinary
Tongtong Wang, Weibo Gong, Xin He, Zuhao Kou, Gang Tan, Shaojun Zhou, Hongtao Yu, Maohong Fan, Harold H. Kung
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2020)
Review
Biochemistry & Molecular Biology
Yoori Kim, Hongtao Yu
EXPERIMENTAL AND MOLECULAR MEDICINE
(2020)
Review
Oncology
Xiaoyan Chen, Angela Winstead, Hongtao Yu, Jiangnan Peng
Summary: Taccalonolides are a new class of microtubule stabilizers derived from plants of the Tacca genus, showing effectiveness against drug-resistant tumors. Through structural modification via semi-synthesis, their anti-proliferation potency can be significantly increased. The unique mechanism of covalently binding to microtubules and resistance circumvention mechanisms make taccalonolides a promising area of research in cancer therapy.
Article
Environmental Sciences
Yuhan Cao, Xiaoping Li, Feng He, Xuemeng Sun, Xu Zhang, Tao Yang, Jie Dong, Yu Gao, Qishang Zhou, Danqian Shi, Jiwen Wang, Hongtao Yu
Summary: A study on the status of toxic metals in the soil-dust-plant total environment of a Coal-Gas industrial city in NW China found that different toxic metals exhibited varied occurrences in different media, with coal combustion and industrial activities identified as the most significant sources of risk.
Article
Environmental Sciences
Xiaoping Li, Xiangyang Yan, Ting Wu, Xu Zhang, Hongtao Yu
Summary: The study revealed that fine fractions of <1 micron in contaminated soil accumulated more PTMs, with higher mobility and bioaccessibility. It is suggested that further environmental management is necessary to prevent the risk of PTMs from Pb-bearing micro-nano size fractions in industrial contaminated soil.
Article
Water Resources
Xiaoping Li, Hameed Ullah, Xuemeng Sun, Xiangyang Yan, Jie Dong, Yu Gao, Yuhan Cao, Tao Li, Hongtao Yu
Summary: This study investigates the spatial distribution, pollution status, and health risks of potentially toxic metals (PTMs) in urban soil, street dust, and plants in Lanzhou, China. The findings reveal that priority metals from traffic sources show similar transfer trends among soil-dust-plant environments, and coal combustion and industrial emissions are important sources of metals. The study highlights the high carcinogenic risk of toxic metals for children, emphasizing the need for attention to lead contamination and monitoring of children aged 0-6 years old.
EXPOSURE AND HEALTH
(2022)
Article
Cell Biology
Haishan Gao, Faxiang Li, Zhejian Ji, Zhubing Shi, Yang Li, Hongtao Yu
Summary: The conserved ATPase p97/Cdc48 mediates cellular processes by unfolding polyubiquitinated proteins. Recent studies have found that p97/Cdc48 can form double hexamers with ordered pore loops, facilitating substrate unfolding and engagement.
Article
Chemistry, Multidisciplinary
Xiaoyan Chen, Hua Deng, Janai A. Heise, David P. Puthoff, Nabeel Bou-Abboud, Hongtao Yu, Jiangnan Peng
Summary: With the increasing interest in medicinal use and the legalization of cannabis, the cannabis marketplace and farming industry have experienced tremendous growth. A high-performance liquid chromatography (HPLC) method was developed and validated to analyze 104 hemp bud materials from 20 varieties in Maryland, comparing and discussing the THC content in different varieties to identify those with high yield and consistency for cannabinoid production.
Article
Cell Biology
Kai Ma, Man Luo, Guanglei Xie, Xi Wang, Qilin Li, Lei Gao, Hongtao Yu, Xiaochun Yu
Summary: This study reveals that noncoding RNAs, specifically rRNA species, associate with and play an important role in mitotic chromosomes. The proteins Ki-67 and its FHA domain and repeat region are responsible for recognizing and anchoring rRNAs to the chromosomes. Inhibition of chromosome-bound rRNAs impairs mitotic chromosome dispersion.
Article
Cell Biology
Heng Liu, Maya M. Polovitskaya, Linlin Yang, Meiling Li, Hongyu Li, Zhen Han, Jianguo Wu, Qiansen Zhang, Thomas J. Jentsch, Jun Liao
Summary: This study reports the 3D structure of human LRRC8A, which can regulate cell volume regulation and ion selectivity, and proposes a mechanism for VRAC activation.
Article
Geochemistry & Geophysics
Ming Zhou, Xiaoping Li, Meng Zhang, Bin Liu, Yuchao Zhang, Yu Gao, Hameed Ullah, Liyuan Peng, Ana He, Hongtao Yu
JOURNAL OF GEOCHEMICAL EXPLORATION
(2020)
