Article
Chemistry, Analytical
Chengli Zhang, Xun You, Yuliang Li, Yuanyuan Zuo, Wei Wang, DanDan Li, ShengWei Huang, Hangzhi Hu, Feng Yuan, Fuqiang Shao, Minggang Yin
Summary: In this study, an aptamer-based amplification strategy using Ce-MOF and m-PdNFs-G4-MBs nanocomposite was developed for the sensitive detection of dopamine (DA). The results showed that the new detection platform achieved a minimum detection limit of 6 pmol/L for DA with good specificity. This platform has the potential to provide a convenient and efficient tool for the diagnosis of multiple neurological disorders in the future.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Adrian Fernandez-Lodeiro, Jamila Djafari, Javier Fernandez-Lodeiro, Maria Paula Duarte, Elisabete Muchagato Mauricio, Jose Luis Capelo-Martinez, Carlos Lodeiro
Summary: In this work, gold nanorods coated with a mesoporous silica shell were successfully prepared and characterized. Conjugation with a model drug, methylene blue, using ultrasound-stimulated loading was explored for potential use in light-mediated antibacterial studies. The lyophilization of this conjugated nanosystem using trehalose showed potent antimicrobial activity against both Gram-negative and Gram-positive bacteria after post-treatment irradiation with a red laser.
Article
Chemistry, Multidisciplinary
Eunsoo Wi, Younghun Kim
Summary: Paper-strip-type methylene blue (MB)-functionalized gold nanoparticles (AuNPs@MB) were developed and tested as colorimetric sensors for arsenic. The valence state of arsenic speciation was pH-dependent. AuNPs@MB exhibited significant agglomeration between nanoparticles via an ion-pairing mechanism with As(V), resulting in a color shift from red to blue. The AuNPs@MB sensor showed high selectivity for As(V) and a solid-state colorimetric sensor based on a cellulose paper strip was also developed.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Irena Maliszewska, Anna Zdubek
Summary: This study compared the efficacy of different Methylene Blue (MB)-based protocols for eradicating biofilms on glass and stainless steel discs by MRSA isolates using a diode laser. The results showed that MB alone had limited bactericidal activity, but the addition of spherical gold nanoparticles increased the destruction of biofilms. A highly efficient protocol involving triple exposure to laser light and the removal of dead bacteria protecting deep layers of pathogens was also described.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jinchen Dong, Tangchun Wang, Enze Xu, Feng Bai, Jun Liu, Zhiliang Zhang
Summary: In this study, hydrophobic SERS substrates were developed by synthesizing gold nanoparticles on cellulose filter paper. The substrates exhibited excellent sensitivity, reproducibility, and stability, and were capable of detecting methylene blue residues in lake water with high sensitivity.
Article
Chemistry, Physical
Thais Lazzarotto Braga, Pollyanna Cristina Vincenzi Conrado, Leticia Graziela Zavadzki Silva, Eduardo Victor Bergmann, Ana Claudia Pedrozo da Silva, Patricia de Souza Bonfim de Mendonca, Jane Martha Graton Mikcha, Leandro S. Herculano, Luis Carlos Malacarne, Edvani Curt i Muniz, Camila Fabiano de Freitas, Wilker Caetano
Summary: The combination of photodynamic therapy (PDT) and photothermal therapy (PTT) using methylene blue (MB) and gold nanoparticles (AuNPs) has shown promise as an effective treatment for microbial diseases. The use of ABA triblock Pluronic (R) P-123 copolymer as a carrier and stabilizing agent for AuNPs has proven to be efficient in obtaining reproducible samples. The synthesis of AuNPs presented plasmonic resonance peaks ranging from 535 to 554 nm, indicating spherical AuNPs. The combination therapy demonstrated both photodynamic and photothermal effects, with synergy observed in the generation of singlet oxygen and heat. The samples showed significant antifungal and antibacterial activity, making this approach a potential method for treating microbial infections.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Irena Maliszewska, Ewelina Wanarska, Alex C. Thompson, Ifor D. W. Samuel, Katarzyna Matczyszyn
Summary: Gold nanoparticles synthesized by the mycelium of Mucor plumbeus are effective for antimicrobial photodynamic therapy, enhancing the effectiveness of the photosensitizer methylene blue to kill both Gram-positive and Gram-negative bacteria. The nanoparticles prevent fast photobleaching, thus improving the bactericidal effect of methylene blue.
Article
Chemistry, Inorganic & Nuclear
Yulin Xie, Man Wang, Qianqian Sun, Dongmei Wang, Shuiping Luo, Chunxia Li
Summary: In this study, a PtBi-beta-CD-Ce6 nanoplatform was developed to generate sustained O-2 for enhanced tumor therapy. The nanoplatform combines photodynamic therapy, photothermal therapy, photoacoustic imaging, and X-ray computed tomography imaging, showing potential for multimodal imaging-guided therapy.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Physical
Erica G. A. de Miranda, Victor H. Toledo, Carolina G. dos Santos, Fanny Costa, Maria Diaz-Lopez, Thiago B. de Queiroz, Otaciro R. Nascimento, Iseli L. Nantes
Summary: Aggregation affects the properties of methylene blue and it can bind with anionic polymer Chelex & REG; 100 through electrostatic interactions. The formation of aggregates modifies the mobility of water molecules and leads to the photobleaching of methylene blue. The resulting leuco form can be used as a reducing agent for the synthesis of nanoparticles with potential applications in optoelectronic devices. Evaluation: 7/10.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Biochemical Research Methods
Rezvan Yazdian-Robati, Narges Hedayati, Shahrzad Dehghani, Mohammad Ramezani, Mona Alibolandi, Majid Saeedi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Biosensor technology is evolving with the development of aptasensors, which combine aptamer as a biological recognition element with gold nanoparticles (AuNPs) as probe to achieve high specificity, selectivity, and sensitivity. These sensors are cost-effective and capable of analyzing specific targets on-site using naked eye.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Patrawadee Yaiwong, Siriporn Anuthum, Padchanee Sangthong, Jaroon Jakmunee, Suwussa Bamrungsap, Kontad Ounnunkad
Summary: This study presents the development of a simple, reliable, highly sensitive, and label-free aptasensor based on polyethyleneimine-coated gold nanoparticles (PEI-AuNPs) for the quantification of alpha-fetoprotein (AFP). The aptasensor is fabricated using a disposable ItalSens screen-printed electrode (SPE) and detects AFP concentration through the electrochemical response of toluidine blue (TB) intercalating into the aptamer-modified electrode after binding with the target. The assay exhibits a linear range of detection of 10 to 50000 pg mL(-1) with a limit of detection (LOD) of 9.5 pg mL(-1) in human serum.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Hao Wang, Bianbian Zhao, Yufei Ye, Xiaoyu Qi, Yuting Zhang, Xiaole Xia, Xiaoli Wang, Nandi Zhou
Summary: In this study, a fluorescence and surface-enhanced Raman scattering dual-mode aptasensor was developed for sensitive and rapid detection of Ochratoxin A (OTA). The aptasensor demonstrated low detection limits in both FL and SERS modes, as well as high specificity and good interferent resistance. It showed great potential for OTA detection in coffee and wine samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Applied
O. V. Ovchinnikov, M. S. Smirnov, T. A. Chevychelova, A. I. Zvyagin, A. S. Selyukov
Summary: The analysis focused on the nonlinear optical properties of thiazine dye molecules of Methylene Blue (MB+), which were located in the near-field zone of spherical gold nanoparticles coated with a silicon dioxide shell (Au/SiO2 NPs). The study found an increase in reverse saturated absorption of MB+ dye in the presence of gold nanoparticles under specific laser pulses, and discussed the plasmon nanoparticles' effect on optical transitions. It was concluded that the increase in effective cross section for triplet-triplet absorption of MB+ molecules in the presence of Au/SiO2 NPs contributed significantly to the observed effect.
Article
Chemistry, Physical
Beibei Xie, Haibo Peng, Ruixue Zhang, Chenhui Wang, Yun He
Summary: A new ultrasensitive electrochemical aptasensor was developed for detecting tetracycline (TET) residue, with a larger linear range and lower detection limit than previous sensors. The new material NCA@SL-AuNPs also exhibited excellent performance in detecting TET in milk.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Engineering, Electrical & Electronic
Mahmoud Roushani, Zahra Mirzaei Karazan
Summary: A novel electrochemical sensor for the determination of Pantoprazole (PAN) was prepared by electropolymerization of dopamine (DA) on the surface of a glassy carbon electrode (GCE). The sensor showed acceptable reproducibility, sensitivity, and selectivity, with a linear range of 0.005 to 1.2 μM and a limit of detection of 1.3 nM. The proposed sensor was successfully used to detect PAN in blood serum samples.
IEEE SENSORS JOURNAL
(2022)
Article
Food Science & Technology
Shekoufeh Yousefi-Nejad, Kobra Heidarbeigi, Mahmoud Roushani
Summary: This study successfully predicted the concentration of crocin in saffron using an electronic tongue system and an artificial neural network model, indicating high accuracy of the developed methods.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Materials Science, Multidisciplinary
Mahmoud Roushani, Masoumeh Sarabaegi, Hadi Hosseini, Fazel Pourahmad
Summary: In this study, a synthesis method for gold nanostructures supported on hollow carbon N-doped nanocapsules was established for the first time. The as-prepared Au@HNC nanocapsules were morphologically studied and characterized using various techniques. These nanocapsules were then used for the ultra-sensitive and fast detection of Helicobacter pylori due to their excellent biocompatibility, high electronic conductivity, and large specific surface area.
JOURNAL OF MATERIALS SCIENCE
(2022)
Article
Chemistry, Analytical
Mahmoud Roushani, Neda Zalpour
Summary: In this study, a new artificial receptor biosensor was successfully designed using a hybrid method of molecularly imprinted polymer and aptamers for ultra-trace Trypsin enzyme recognition. The biosensor showed unparalleled selectivity and sensitivity in real samples, with a good diagnostic linear range and detection limit. The efficiency of the biosensor was significantly improved with the presence of aptamers in the system.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Zeinab Rahmati, Mahmoud Roushani, Hadi Hosseini
Summary: In recent years, the combination of aptamers with new nanomaterials has significantly improved the performance of biosensors, particularly in the design of hollow carbon-based materials. This study successfully developed hierarchical porous nickel hydroxide nanosheets on hollow nitrogen-doped carbon nanoboxes, demonstrating sensitive detection of alpha-fetoprotein content.
Article
Biochemistry & Molecular Biology
Masoumeh Nosrati, Mahmoud Roushani
Summary: This study presented a 3D-structural model for a 79-mer ss-DNA aptamer and predicted its binding pocket using molecular docking and dynamics simulation. The results revealed the molecular details of the interaction between streptomycin and aptamer, providing insights for optimizing the aptamer's efficiency in biosensing applications.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Engineering, Electrical & Electronic
Masoumeh Sarabaegi, Mahmoud Roushani, Hadi Hosseini, Zahra Saedi, Ensieh Ghasemian Lemraski
Summary: A specific and sensitive aptasensor for Helicobacter pylori (H. pylori) was designed using a modified electrode with a nano-sized bimetallic NiCo-metal-organic frameworks nanostructure (NiCo-MOF@C) as platform. Due to hydrogen bonds, a large amount of aptamer strands could bind to NiCo-MOF@C, resulting in a detection limit of 1 CFU.ml-1 and a linear range of 101-107 CFU.ml-1. The proposed aptamer-based biosensor showed specificity, low detection limit, extended linear detection range, and stability for H. pylori.
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
(2022)
Article
Chemistry, Analytical
Mahmoud Roushani, Somayeh Farokhi, Zeinab Rahmati
Summary: In this study, a new biological biodevice for the accurate and cost-effective measurement of aflatoxin B1 (AFB1) was proposed. The device is based on a hybrid of molecular imprinting polymer (MIP) and aptamer (Apt) on a glassy carbon electrode modified by Cu2O nanocubes (Cu2O NCs/GCE). The device exhibited high performance, with a linear range of 50.0 pg L-1 to 3.5 ng L-1 and a detection limit of 3.5 ng L-1 to 40.0 ng L-1 and 12.0 pg L-1. The advantages of the device include novelty, high selectivity, portability, and cost-effectiveness.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Mahmoud Roushani, Alireza Veysi, Hossein Najafi
Summary: The inhibiting effect of adenine on the corrosion of 304 and 316 stainless steels in hydrochloric acid solution was studied. The results showed that the addition of adenine significantly reduced the corrosion rate of the stainless steels, with good inhibiting efficiency. Surface characterization and electrochemical methods revealed that adenine could form a protective film on the surface of the stainless steels, providing corrosion protection.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2023)
Article
Engineering, Electrical & Electronic
Zeinab Rahmati, Mahmoud Roushani, Hadi Hosseini
Summary: Preparation of hierarchical nanostructures based on MOFs-derived TMPs has attracted much attention for its attractive properties in electrochemical sensors, particularly in cortisol detection. In this research, a label-free and ultrasensitive electrochemical aptasensor using MOF-derived Ni-P nanorods was developed. The designed aptasensor exhibited a wide linear sensing range between Rct and increasing cortisol concentration, with a low detection limit of 10 aM. The efficiency of the aptasensor was evaluated using real urine samples and compared with the ELISA method, showing satisfactory results.
IEEE SENSORS JOURNAL
(2023)
Article
Chemistry, Analytical
Somayeh Farokhi, Mahmoud Roushani
Summary: Flower-like core-shell nanostructures based on natural asphalt (NA) coated with nickel-layered double hydroxide nanosheets (Ni-LDH NSs) were synthesized for the first time and used as an efficient platform in the design of sarcosine (SAR) electrochemical aptasensor for prostate cancer (PCa) diagnosis. The presented SAR aptasensor showed a promising performance with a linear concentration range and a low limit of detection (LOD). This study can contribute to the development of PCa diagnostic aptasensors and the exploration of NA nanostructures in other electrochemical applications.
Article
Biochemistry & Molecular Biology
Nagham Mahdi, Mahmoud Roushani, Zahra Mirzaei Karazan
Summary: This research developed a sensor based on molecularly imprinted polymer (MIP) for the precise detection of riboflavin (RF), dopamine (DA), and L-tryptophan (Trp) using the electropolymerization method. The MIP/GCE sensor exhibited good selectivity, reproducibility, and stability. It successfully detected RF, DA, and Trp in serum and milk samples with satisfactory results.
JOURNAL OF MOLECULAR RECOGNITION
(2023)
Article
Chemistry, Analytical
Neda Zalpour, Mahmoud Roushani
Summary: One of the cutting-edge emerging approaches in sensors research is the design of flexible, portable, selective sensors that can quickly and accurately identify trace quantities of target molecules. In this study, carbon cloth was used as a binder-free 3D flexible material for the working electrode, and AuCD nanoparticles were synthesized and used to modify the carbon cloth surface. The molecular imprinting polymer approach was utilized to fabricate an imprinted sensor. The optimized sensor exhibited excellent performance in accurately identifying the target molecule.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Somayeh Farokhi, Mahmoud Roushani, Zahra Saedi
Summary: This study reports a new electrochemical strategy for selective and accurate detection of SRN in urine samples. The use of CuCo2O4 nanosheets as a platform improves the diagnostic performance of the electrochemical aptasensor.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Somayeh Farokhi, Mahmoud Roushani, Hadi Hosseini
Summary: In this paper, NiCo-X (X = OH, S, P, Se) nanodiscs were synthesized through hydrothermal method and subsequent treatments. The electrocatalytic performance of these nanodiscs in the determination of isoprenaline (IP) was compared. The results showed that NiCo-Se NDs exhibited better electrocatalytic activity and were successfully used for measuring IP in human blood serum samples.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
