Article
Biophysics
Yan Yang, Di Wu, Jianghua Liu, Zhuoqun Su, Lin Li, Yongning Wu, Guoliang Li
Summary: We developed a highly sensitive and portable dual-mode sensor for the detection of Aflatoxin B1 in food samples. The sensor utilizes aptamer recognition and enzyme-free catalyzed hairpin assembly to achieve signal amplification, as well as surface-enhanced Raman scattering for visualization. This strategy offers a novel approach for the sensitive detection of mycotoxins and has been successfully applied to real samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Agriculture, Multidisciplinary
Gu Shi, Chong Yan, Junhua Chen
Summary: Researchers developed an ultrasensitive aptasensor for the detection of microcystin-LR based on the assembly of Y-shaped hairpins. The cyclic assembly reactions resulted in a high fluorescence signal, enabling the detection of microcystin-LR with a low limit of 0.2 pM.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhen Liu, Chen Liu, Liqiong He, Jinquan Liu, Le Li, Shengyuan Yang, Yan Tan, Xing Liu, Xilin Xiao
Summary: An ultrasensitive DNAzyme-based fluorescence biosensor for detecting Cu2+ was designed using cascade signal amplification strategy. The detection system utilized the activation of Cu2+-dependent DNAzyme by Cu2+ and subsequent reactions to generate a strong fluorescent signal. The biosensor showed excellent detection performance and potential applications.
Article
Biotechnology & Applied Microbiology
Jesus Camara-Almiron, Laura Dominguez-Garcia, Nadia El Mammeri, Alons Lends, Birgit Habenstein, Antonio de Vicente, Antoine Loquet, Diego Romero
Summary: This study demonstrates that recombinantly purified RcTasA retains the biochemical properties of the entire protein and shows that two aggregation-prone stretches and imperfect amino acid repeats in RcTasA contribute to its amyloid-like capacity. The study also identifies the critical role of residues D64, K68, and D69 in the structural function of TasA. Experiments with mutant versions of TasA reveal a partial loss of function in the assembly of the extracellular matrix or the stability of the core and amyloid-like properties. Overall, these findings provide insights into the polymerization process of TasA and the sequence determinants that govern the behavior of protein filaments in bacteria.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Chemistry, Analytical
Liyuan Yu, Xiaoqian Zhang, Daobin Jin, Fangxu Lou, Jikuan Zhao, Xu Hun
Summary: A chemiluminescence (CL) sensing strategy for kanamycin residue detection in fish samples was developed using luminol-functionalized gold nanoparticles as CL nanoprobe materials, combined with DNA hairpin structure and carboxyl-modified magnetic beads. This method showed excellent sensitivity and a good linear relationship, providing a low-cost and high-sensitivity CL analysis strategy.
Article
Chemistry, Analytical
Hua Chai, Mingyuan Wang, Longhai Tang, Peng Miao
Summary: An ultrasensitive electrochemical biosensor for miRNA assay was developed in this study, showing remarkable sensitivity and a wide linear range, as well as the capability to specifically discriminate interfering miRNAs with slightly different sequences.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Environmental
Xiaohai Yan, Yuning Zhao, Gengan Du, Qi Guo, Hong Chen, Qian He, Qiannan Zhao, Huanfeng Ye, Jianlong Wang, Yahong Yuan, Tianli Yue
Summary: Researchers have developed a simple, effective, and sensitive fluorescent aptasensor for the detection of low molecular weight mycotoxin patulin in apple juice. The aptasensor utilizes sulfur quantum dots encapsulated in MOF-5-NH2 as a fluorescent probe and self-cycling catalyzed hairpin assembly for signal amplification. The aptasensor demonstrates high sensitivity and satisfactory specificity, anti-interference, and reproducibility.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Tianjin Xie, Yuxin Liu, Jiali Xie, Yujie Luo, Kai Mao, Chengzhi Huang, Yuanfang Li, Shujun Zhen
Summary: In this study, a high molecular weight and volume DNA dendrimer was used as a new fluorescence anisotropy (FA) amplifier coupled with target-catalyzed hairpin assembly (CHA) for sensitive detection of miRNA-21. The method showed high sensitivity and selectivity and could be used to detect other biomolecules by changing the nucleic acid probe.
Article
Biochemical Research Methods
Shanshan Qin, Xuliang Chen, Zhichen Xu, Tao Li, Shuhong Zhao, Rui Hu, Jiang Zhu, Ying Li, Yunhuang Yang, Maili Liu
Summary: Researchers have reported a new RNA detection method based on ht-G3/ThT, which achieved significantly improved sensitivity for non-amplified RNA targets by introducing a target recycling strategy and successfully designing a molecular beacon for the SARS-CoV-2 N gene.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Su Jiang, Qian Liu, Juan Hu, Dong Yuan, Yan Zhang, Chun-yang Zhang
Summary: In this study, a novel method was developed for sensitive detection of miRNA in lung tissues by target-triggered assembly of G-quadruplex DNAzyme nanowires. This method exhibited high sensitivity, a large dynamic range, and the ability to distinguish single-base mutations, holding promising applications in biomedical analysis and cancer prognosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
Kathleen L. Vergunst, Calem Kenward, David N. Langelaan
Summary: Hydrophobins are small proteins secreted by fungi that accumulate at interfaces, modify surface hydrophobicity, and self-assemble into large amyloid-like structures. This study presents the atomic resolution structure of hydrophobin SLH4 and compares its properties with another hydrophobin, SC16. Despite having similar structures, SLH4 and SC16 exhibit distinct rodlet morphology, self-assembly propensity, and preferred assembly conditions. Analysis suggests that conformational changes in residues in the first intercysteine loop play a role in rodlet formation. This research provides insights into the structure and function of hydrophobins and their potential applications as surface modifiers.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Linyan Li, Xiaojun Fang, Jingqing Le, Yanhui Zheng, Xiarong Tan, Zhou Jiang, Hongxia Li, Jianguo Xu, Huo Xu
Summary: In this study, a cascade catalytic hairpin assembly (CCHA) method was developed for sensitive and selective detection of miRNA. The method showed high assay sensitivity, excellent sequence specificity, and the ability for intracellular imaging, demonstrating promising potential for biosensing, clinical diagnosis, or prognosis.
Article
Chemistry, Analytical
Xiaoyan Sun, Shilong Dong, Wenying Zhao
Summary: An ultrasensitive electrochemical biosensor based on catalytic hairpin assembly and DNAzyme signal amplification strategy is proposed for the detection of lead ions. The biosensor utilizes polyethyleneimine-reduced graphene oxide combined with gold@silver nanosheets as electrode substrate modification materials. The biosensor demonstrates a good linear relationship and low detection limit, enabling trace detection of lead ions in tap water samples and providing a platform for the detection of other targets.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Terefe Tafese Bezuneh, Tadesse Haile Fereja, Shimeles Addisu Kitte, Haijuan Li, Yongdong Jin
Summary: An enzyme-free target-triggered catalytic hairpin assembly (CHA) was proposed for signal amplified fluorescence thrombin detection, demonstrating a limit of detection (LOD) of 165 pM and linear range from 0.2 to 100 nM. The method could be potentially applied in complex biological matrices for sensitive detection of thrombin.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Xuejiao Yang, Liquan Yuan, Yue Xu, Bingfang He
Summary: A colorimetric sensing strategy was proposed for sensitive miRNA analysis, utilizing nucleic acid isothermal amplification and enzyme-catalyzed chromogenic reaction. The developed method exhibited high sensitivity and specificity, with a detection limit of 90.3 fM for miRNA-21. The sensing platform was successfully employed for early cancer diagnosis through the detection of miRNA-21 in spiked serum.
ANALYTICA CHIMICA ACTA
(2023)