Article
Multidisciplinary Sciences
Sylvain Trigueros, Thomas Brauge, Tommy Dedole, Sabine Debuiche, Veronique Rebuffel, Sophie Morales, Pierre R. Marcoux, Graziella Midelet
Summary: The innovative approach of Raman-DIP allows for the evaluation of bacteria metabolism and identification of anabolic pathways using deuterated carbon source. However, the use of heavy water treatment may affect the viability of bacteria. This study evaluated the effect of heavy water incorporation on the viability state of Listeria innocua cells.
Review
Microbiology
Kieu The Loan Trinh, Nae Yoon Lee
Summary: Viability assessment is crucial for evaluating bacterial pathogens, and current methods have limitations. Advances in microfluidic technology offer possibilities for improving these assessments.
Article
Environmental Sciences
Xiang Xiao, En-Jing He, Xue-Rong Lu, Li-Jun Wu, Yang-Yang Fan, Han-Qing Yu
Summary: The study shows that silver nanoparticles possess strong antibacterial ability, leading to the majority of bacteria entering a viable but non-culturable state. Environmental factors such as Cl- and illumination can affect the solubility of nanoparticles and thereby impact the survival state of bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
S. Copin, J. Mougin, V. Raguenet, A. Robert-Pillot, G. Midelet, T. Grard, M. Bonnin-Jusserand
Summary: Vibrio sp., commonly found in aquatic ecosystems, play important roles in human health and aquaculture. This study suggests that PMA-qPCR methods may be more effective in detecting the viable state of Vibrio cells.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Annalisa Visciglia, Serena Allesina, Angela Amoruso, Annachiara De Prisco, Raja Dhir, Peter A. Bron, Marco Pane
Summary: The number of live bacterial cells is a key parameter for assessing the quality of probiotic products. Plate counting is the standard method used in the industry, but it may underestimate viable but not cultivable cells. Flow cytometry is a culture-independent method that can accurately count live, damaged, and dead cells, making it a powerful tool for comprehensive monitoring of probiotic products.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ziyi Cheng, Xiaoli He, Zufang Wu, Peifang Weng
Summary: This study focused on the fermentation performance of Lactobacillus fermentum Lf01 and prepared it as powder using vacuum freeze-drying technology. Response surface methodology was used to optimize the composition of the mixture for protecting the powdered L. fermentum. The results showed that a mixture containing 10% skim milk, 12% sucrose, 0.767% tyrosine, and 2.033% sorbitol ensured the highest survival rate. The potential mechanism of the protective effect of complex protectants on L. fermentum was explored through electron microscopy, Fourier transform infrared spectroscopy, and transcriptomics. The study identified differential genes that were up-regulated and found that genes involved in various metabolic processes were significantly affected. These findings provide new insights into the mechanism of lyoprotectants and have important practical applications for strain preservation and starter production.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Review
Biology
Dustin A. Ammendolia, William M. Bement, John H. Brumell
Summary: Plasma membrane integrity is crucial for cellular homeostasis, and cells have repair pathways to restore membrane integrity when damaged. Tissue-specific stressors and membrane repair pathways play important roles in health and disease. Genetic and environmental factors impact plasma membrane integrity and contribute to disease pathogenesis in various tissues.
Article
Food Science & Technology
Franca Rossi, Ilaria Del Matto, Paola Manocchio, Maria Antonietta Saletti, Luciano Ricchiuti, Lucio Marino
Summary: The aim of this study was to investigate the survival of Campylobacter jejuni in dairy matrices by viability qPCR. The cheese outbreak strain, C. jejuni 11 218, showed enhanced tendency to become viable but nonculturable (VBNC) in UHT milk, as well as in raw and fermented milk and cheese. Viability qPCR also detected viable Campylobacter spp. in 40.9% of 25 mL raw milk samples that were negative on culture. The results highlight the value of viability qPCR in estimating Campylobacter risk in dairy products.
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
(2023)
Review
Oncology
Syrina Fred Mehrabi, Sabina Elmi, Jesper Nylandsted
Summary: The limitations of current cancer therapies, including multidrug resistance, have led to the exploration of repurposing existing drugs. Phenothiazines, primarily used as antipsychotic agents, show potential in impacting key mechanisms driving tumor growth and metastasis by altering lipid composition, modifying calcium channels, and inducing protein accumulation. This review provides a comprehensive overview of the anticancer activities of phenothiazines, such as trifluoperazine, prochlorperazine, chlorpromazine, promethazine, thioridazine, and fluphenazine, and highlights their potential in improving future cancer treatment.
FRONTIERS IN ONCOLOGY
(2023)
Article
Cell Biology
Eneko Madorran, Andraz Stozer, Zoran Arsov, Uros Maver, Jan Rozanc
Summary: Determining cell viability is a challenge due to uncertainties. A novel method involving the measurement of cell membrane electrical potential shows promising results in directly determining cell viability. The method demonstrates good accuracy when compared to established methods and is compatible with live cell imaging.
Article
Plant Sciences
Malgorzata Lichocka, Magdalena Krzymowska, Magdalena Gorecka, Jacek Hennig
Summary: Studies investigated the role of plant annexin 5 (ANN5) in maintaining membrane integrity in pollen grains, showing that ANN5 is involved in repairing cell membrane damage, especially under osmotic or ionic imbalances.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemical Research Methods
Marco Eigenfeld, Leonie Wittmann, Roland Kerpes, Sebastian Schwaminger, Thomas Becker
Summary: For industrial processes, it is crucial to have a fast, precise, and reliable method for determining the physiological state, particularly viability, of yeast cells. This study examined the impact of magnetic nanoparticles (MNPs) on yeast cell viability and the assay itself, testing two types of yeast cells. The results showed that traditional colorimetric staining was only reliable for cells with good viability, while fluorescence microscopy and flow cytometry-based assays demonstrated high precision and reliability in determining viability, even in the presence of MNPs. Moreover, the study revealed a non-linear relationship between membrane fluidity and yeast cell viability. The use of CFDA and membrane fluidity provided accurate results for both yeast types, allowing for efficient viability determination in experiments involving yeast cell manipulation or separation using MNPs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Luigi Sansone, Antonio de Iure, Mario Cristina, Manuel Belli, Laura Vitiello, Federica Marcolongo, Alfredo Rosellini, Lisa Macera, Pietro Giorgio Spezia, Carlo Tomino, Stefano Bonassi, Matteo A. Russo, Fabrizio Maggi, Patrizia Russo
Summary: The study investigates the effects of nicotine on inflammatory cytokines expression in cells infected with SARS-CoV-2. Nicotine increases the levels of cytokines and induces severe cellular damage, ruling out its protective role in COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Ga-Eun Lee, Jiin Byun, Cheol-Jung Lee, Yong-Yeon Cho
Summary: The integrity of the nuclear membrane is crucial for cellular function and disease pathogenesis. While there has been extensive research on nuclear membrane assembly, disassembly, and interactions with other cellular components, less emphasis has been placed on the regulation of nuclear morphology through nuclear membrane integrity. This review aims to shed light on the biomolecules responsible for maintaining nuclear membrane integrity and explore the role of type II membrane protein transcription factors in this context.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Chemical
Alena Popova, Sandrine Boivin, Takuji Shintani, Takahiro Fujioka
Summary: This study aimed to produce a high-integrity RO membrane by forming a polyamide skin layer on a TE support layer, in order to enhance the integrity of the membrane and improve the microbiological safety of potable water reuse.
Article
Biochemistry & Molecular Biology
Sagadevan Kalaiselvan, Sathish Sankar, Mageshbabu Ramamurthy, Asit Ranjan Ghosh, Balaji Nandagopal, Gopalan Sridharan
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Article
Biotechnology & Applied Microbiology
Alok Kumar Mishra, Asit Ranjan Ghosh
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2018)
Article
Biotechnology & Applied Microbiology
Kandasamy Sasikumar, Asit Ranjan Ghosh, Azger Dusthackeer
Article
Biotechnology & Applied Microbiology
V. Dubey, A. K. Mishra, A. R. Ghosh, B. K. Mandal
JOURNAL OF APPLIED MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Budhaditya Ghosh, Gowri Sukumar, Asit Ranjan Ghosh
FOLIA MICROBIOLOGICA
(2019)
Article
Biochemical Research Methods
Vinay Dubey, Alok Kumar Mishra, Asit Ranjan Ghosh
JOURNAL OF PROTEOMICS
(2020)
Article
Medicine, Research & Experimental
Vinay Dubey, Alok Kumar Mishra, Asit Ranjan Ghosh
Summary: This study focused on the role of biohydrogenation-derived conjugated linoleic acid (CLA) of probiotic Pediococcus pentosaceus GS4 (CLA(GS4)) in colon cancer (CC) treatment. CLA(GS4) induced peroxisome proliferator-activated receptor gamma (PPAR gamma) expression in human CC HCT-116 cells and promoted apoptosis through altering cell metabolism. These findings suggest the potential therapeutic efficacy of CLA(GS4) in colon cancer.
Article
Pharmacology & Pharmacy
Kandasamy Sasikumar, Vinay Dubey, Asit Ranjan Ghosh
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
Letter
Pharmacology & Pharmacy
Kandasamy Sasikumar, Asit Ranjan Ghosh
BANGLADESH JOURNAL OF PHARMACOLOGY
(2019)
Article
Microbiology
Alok Kumar Mishra, Shravanthi S. Kumar, Asit Ranjan Ghosh
FEMS MICROBIOLOGY LETTERS
(2019)
Article
Immunology
Kumaran Vadivel, Mageshbabu Ramamurthy, Sathish Sankar, Amita Jain, Padma Srikanth, Asit Ranjan Ghosh, Balaji Nandagopal, Aravindan Nair, Gopalan Sridharan
INDIAN JOURNAL OF MEDICAL RESEARCH
(2017)
Article
Tropical Medicine
Mayur Bagad, Ram Pande, Vinay Dubey, Asit Ranjan Ghosh
ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE
(2017)
Letter
Pharmacology & Pharmacy
Kandasamy Sasikumar, Asit Ranjan Ghosh
BANGLADESH JOURNAL OF PHARMACOLOGY
(2017)
Article
Electrochemistry
K. S. Shalini Devi, V. T. Mahalakshmi, Asit Ranjan Ghosh, Annamalai Senthil Kumar
ELECTROCHIMICA ACTA
(2017)