Article
Plant Sciences
Jin Liang, Wenping Jiang
Summary: This paper proposes a ResNet50-DPA model for tomato leaf disease identification. By introducing an improved ResNet50 and a dual-path attention mechanism, it can capture key features more accurately and improve the accuracy of disease identification. In addition, incorporating the DPA module into the residual module can also reduce economic losses.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Seongho Jeong, Seongkyun Jeong, Jaehwan Bong
Summary: Due to climate change, plant diseases and pests are spreading at a faster rate, with the tomato leaf miner being a major concern. Regular inspections throughout the tomato's life cycle are necessary to prevent losses caused by the tomato leaf miner. Research on deep neural network models for detecting the tomato leaf miner found that the segmentation model outperformed the classification model, providing higher precision, recall, and F1-score values.
Article
Agronomy
Ding Peng, Wenjiao Li, Hongmin Zhao, Guoxiong Zhou, Chuang Cai
Summary: In this study, we propose a novel classification network called DIMPCNET for tomato leaf disease, which overcomes the challenges posed by complex backgrounds, small differences between diseases, and large differences within diseases. By collecting 1256 original images of 5 tomato leaf diseases and expanding them to 8190 using data enhancement techniques, we were able to achieve high recognition accuracy and F1-score (94.44% and 0.9475 respectively) with a loss of approximately 0.28%. This advanced method provides a new idea for crop disease identification, such as tomatoes, and the development of smart agriculture.
Article
Computer Science, Information Systems
Antonio Guerrero-Ibanez, Angelica Reyes-Munoz
Summary: Agriculture is important for Mexico's economy, with the agricultural sector contributing 2.5% to the country's GDP. Tomatoes are the most exported agricultural product, highlighting the need for improved crop yields. This study proposes a model based on convolutional neural networks for identifying and classifying tomato leaf diseases, using a combination of a public dataset and additional field photographs. The results demonstrate the model's high performance, achieving over 99% accuracy in both the training and test datasets.
Article
Plant Sciences
Theo Poucet, Maria Begona Gonzalez-Moro, Cecile Cabasson, Bertrand Beauvoit, Yves Gibon, Martine Dieuaide-Noubhani, Daniel Marino
Summary: This study found that under ammonium nutrition, the carbon and nitrogen metabolism in plants varied at different leaf positions, suggesting a trade-off between NH4+ accumulation and assimilation to protect young leaves from ammonium stress. Additionally, plants supplied with ammonium showed changes in carbon partitioning, potentially regulated by the biochemical pH-stat.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Deding Su, Wei Xiang, Qin Liang, Ling Wen, Yuan Shi, Bangqian Song, Yudong Liu, Zhiqiang Xian, Zhengguo Li
Summary: Leaf morphogenetic activity determines leaf shape diversity. In this study, a transcription factor called SlBES1.8 in tomato was found to promote leaf pattern simplification by regulating the gibberellin (GA) signaling pathway. Overexpression of SlBES1.8 in tomato plants reduced sensitivity to exogenous GA treatment and increased sensitivity to a GA biosynthesis inhibitor. Furthermore, SlBES1.8 directly regulated genes involved in GA metabolism and signaling, and its activity was inhibited by the interaction with a negative regulator called SlDELLA.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Agronomy
Yang Wu, Lihong Xu
Summary: An Adversarial-VAE network model is proposed to generate images of tomato leaf diseases and expand the training set for improved model accuracy. Experimental results show superior image quality compared to other methods, and effective improvement in identification accuracy.
Article
Biochemistry & Molecular Biology
Ming-Jie Li, Guo-Fang Jiang, Wei Wang
Summary: This study analyzed the metabolic changes in K. inachus during different stages and diapause using a non-targeted metabolomics analysis. Glycerophospholipids, amino acids and their derivatives, and fatty acyls were found to be crucial in the developmental stages of K. inachus. Significant changes in energy utilization and management were observed during adult diapause, including the accumulation of glycerophospholipids and carbohydrates, degradation of lipids, and shifts in amino acid and its derivatives. Pathway analyses also revealed the association of alpha-linolenic acid metabolism and ferroptosis with adult diapause.
Article
Agronomy
Anil Bhujel, Na-Eun Kim, Elanchezhian Arulmozhi, Jayanta Kumar Basak, Hyeon-Tae Kim
Summary: Plant disease recognition is crucial for protecting crops from losses. In this study, a lightweight convolutional neural network with attention modules was developed to improve accuracy. The results showed that attention mechanisms enhanced the precision, recall, and overall accuracy of the models, and the lightweight model outperformed the standard model.
Article
Multidisciplinary Sciences
Hongxia Deng, Dongsheng Luo, Zhangwei Chang, Haifang Li, Xiaofeng Yang
Summary: Accurate recognition of tomato diseases is crucial for agricultural production. The new data augmentation method RAHC_GAN based on generative adversarial networks can generate leaves with obvious disease features, significantly improving recognition performance. This method can also be applied to solve the problem of insufficient data in other plant research tasks, as shown in the results of apple, grape, and corn datasets.
Review
Plant Sciences
Ahmed H. El-Sappah, Shiming Qi, Salma A. Soaud, Qiulan Huang, Alaa M. Saleh, Mohammed A. S. Abourehab, Lingyun Wan, Guo-ting Cheng, Jingyi Liu, Muhammad Ihtisham, Zarqa Noor, Reyazul Rouf Mir, Xin Zhao, Kuan Yan, Manzar Abbas, Jia Li
Summary: Tomato yellow leaf curl virus (TYLCV) is a highly harmful disease that affects tomato growth and production worldwide. Six resistance genes have been transferred to commercial tomato cultivars from wild germplasms, providing protection against TYLCV. However, only three of these resistance genes have shown significant levels of resistance. Researchers have cloned these resistance genes and studied their antiviral mechanisms, aiming to maintain and spread resistance to TYLCV globally.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mengliang Zhao, Yanjing Ren, Wei Wei, Jiaming Yang, Qiwen Zhong, Zheng Li
Summary: Jerusalem artichokes, a perennial crop with high drought tolerance and value, have been studied for their metabolomic response to drought stress using ultra-performance liquid chromatography and tandem mass spectrometry. The study identified differential metabolites involved in secondary metabolism and amino acid biosynthesis, providing insights for breeding drought-resistant varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Yu Xin Wen, Jia Yi Wang, Hui Hui Zhu, Guang Hao Han, Ru Nan Huang, Li Huang, Yi Guo Hong, Shao Jian Zheng, Jian Li Yang, Wei Wei Chen
Summary: In this study, the researchers report the key role of the methylation enzyme SlDRM7 in tomato plant growth and development, particularly in leaf interveinal chlorosis and senescence. Silencing of SlDRM7 led to developmental defects and alterations in methylation and gene expression levels of genes involved in chlorophyll synthesis, photosynthesis, and starch degradation. SlDRM7 was also found to be activated during aging- and dark-induced senescence.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Shengyi Zhao, Yun Peng, Jizhan Liu, Shuo Wu
Summary: Crop disease diagnosis is crucial for crop yield and agricultural production. This study proposed a deep convolutional neural network with an attention mechanism for accurate diagnosis of tomato leaf diseases. Experimental results on both tomato leaf diseases dataset and grape leaf diseases dataset demonstrated the model's high performance in extracting complex disease features.
Article
Biochemistry & Molecular Biology
Camilla Sacco Botto, Slavica Matic, Amedeo Moine, Walter Chitarra, Luca Nerva, Chiara D'Errico, Chiara Pagliarani, Emanuela Noris
Summary: Drought stress is a major factor affecting agricultural production by negatively impacting plant growth and metabolism. Previous research has shown that viruses, such as the tomato yellow leaf curl virus, can enhance a plant's ability to tolerate both drought and heat stress. However, the underlying mechanisms for this induced drought tolerance need further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Yangan Chen, Patrick P. J. Mulder, Onno Schaap, Johan Memelink, Peter G. L. Klinkhamer, Klaas Vrieling
Summary: Plants produce diverse secondary metabolites as a strategy to cope with environmental stresses, and pyrrolizidine alkaloids are one class of such metabolites with high diversity. In this study, we analyzed the concentration and composition of pyrrolizidine alkaloids in leaves of 17 Jacobaea species and reconstructed their phylogeny. Our results revealed significant inter- and intraspecific variation in pyrrolizidine alkaloid diversity, suggesting species-specific patterns. Ancestral state reconstruction showed random distributions of individual alkaloids, indicating ecological regulation rather than genetic gains or losses during evolution.
JOURNAL OF SYSTEMATICS AND EVOLUTION
(2022)
Article
Microbiology
Pablo Farace, Silvio Cravero, Catalina Taibo, Julian Diodati, Claudia Morsella, Fernando Paolicchi, Julia Sabio y Garcia, Andrea Gioffre
Summary: This study evaluated and characterized the secretion of outer membrane vesicles (OMVs) in Campylobacter fetus, confirming their production in all tested strains. The OMVs showed a spherical shape and variable size, and immunoreactivity was demonstrated. These OMVs could potentially modulate host response and be used as adjuvants in veterinary vaccines.
REVISTA ARGENTINA DE MICROBIOLOGIA
(2022)
Article
Plant Sciences
Rocio Escobar-Bravo, Bernardus C. J. Schimmel, Gaetan Glauser, Peter G. L. Klinkhamer, Matthias Erb
Summary: Herbivore population dynamics are affected by plant-mediated interactions. Leaf herbivory by one herbivore species can affect the performance of conspecific pupae in the soil. This study shows that the aboveground herbivory triggers changes in root defense signaling and volatile production, which can alter the pupal development time.
Article
Agronomy
Jing Zhang, Peter G. L. Klinkhamer, Klaas Vrieling, T. Martijn Bezemer
Summary: The study found that foliar application of SA did not significantly affect the composition of bacterial genera in the rhizosphere of J. vulgaris, but specific genera were significantly impacted in the second, third, and fourth generations. Out of the total 270 bacterial species, 89 (32.4%) were present as the core microbiome in all treatments over four plant cycles.
Article
Plant Sciences
Ricardo Tighe-Neira, Jorge Gonzalez-Villagra, Adriano Nunes-Nesi, Claudio Inostroza-Blancheteau
Summary: Heavy metals and their engineered nanoparticles are emerging contaminants that are known to impact plant growth and development. This critical review focuses on the effects of copper, iron, zinc, and manganese nanoparticles on food crops. Copper and manganese nanoparticles show similar impacts, while iron and zinc nanoparticles appear to be less toxic. Further research is needed to fully understand the physiological, metabolic, and molecular effects of nanoparticles and their ion counterparts on crop plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Jessica A. S. Barros, Joao Henrique F. Cavalcanti, Karla G. Pimentel, David B. Medeiros, Jose C. F. Silva, Jorge A. Condori-Apfata, Taly Lapidot-Cohen, Yariv Brotman, Adriano Nunes-Nesi, Alisdair R. Fernie, Tamar Avin-Wittenberg, Wagner L. Araujo
Summary: This study investigated the metabolic adjustments in plants under carbon starvation and the role of the transcription factor WRKY45. The results showed that overexpression of WRKY45 leads to early senescence and altered amino acid metabolism, and is associated with dysregulation of mitochondrial signaling and activation of alternative respiration.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemical Research Methods
Rutger A. Vos, Catharina A. M. Van der Veen-van Wijk, M. Eric Schranz, Klaas Vrieling, Peter G. L. Klinkhamer, Frederic Lens
Summary: The study developed a Gene Ontology-mediated approach to investigate key genes within QTLs, applied to flowering time in giant woody Jersey kale, identifying genes regulating flowering time through enrichment of the circadian rhythm pathway. Results show that the ontology-mediated approach aids in gene exploration and discovery.
Article
Plant Sciences
Viviane Correa, Jose Goncalves, Karen Costa, Igor Oliveira, Jose Santos, Sabrina Oliveira, Marciel Ferreira, Roberval Lima, Wagner Araujo, Adriano Nunes-Nesi
Summary: The addition of phosphorus to the substrate can improve the morpho-physiological traits of Brazil nut trees in Amazonian soils, leading to increased growth and photosynthetic efficiency.
Article
Agronomy
Debora Durso Caetano-Madeira, Rebeca Patricia Omena-Garcia, Tamiris Lopes Elerati, Camila Batista da Silva Lopes, Thais Roseli Correa, Genaina Aparecida de Souza, Leonardo Araujo Oliveira, Cosme Damiao Cruz, Leonardo Lopes Bhering, Adriano Nunes-Nesi, Weverton Gomes da Costa, Edgard Augusto de Toledo Picoli
Summary: In this study, four commercial eucalyptus clones were evaluated for their response to water stress. The results showed that SuzT clone exhibited higher carbon accumulation and water use efficiency, indicating its better adaptation to drought conditions.
Article
Plant Sciences
Sofia Pontigo, Leyla Parra-Almuna, Ana Luengo-Escobar, Patricia Poblete-Grant, Adriano Nunes-Nesi, Maria de la Luz Mora, Paula Cartes
Summary: This study aimed to identify ryegrass cultivars with contrasting phosphorus (P) use efficiency and assess their associated biochemical and molecular responses. Nine ryegrass cultivars were evaluated under optimal and P-deficient conditions, and the activity and gene expression of acid phosphatases (APases) and P transporters were analyzed. The results showed that cultivars with high P acquisition efficiency were influenced by root-related responses, while cultivars with high P utilization efficiency were influenced by P transporters and APase activity in shoots.
Article
Anthropology
Valeria Arencibia, Marianne Munoz Hidalgo, Cristian Crespo, Lucas Maldonado, Gabriel Lichtenstein, Laura Kamenetzky, Pablo Vera, Atilio F. Zangrando, Augusto Tessone, Sergio Avena, Veronica Lia, Andrea Puebla, Cristina Dejean
Summary: The use of massively parallel sequencing in archaeological samples is challenging due to limited accessibility to the necessary technologies outside of US and European research centers. In this study, collaboration between institutions in Argentina resulted in the successful application of massively parallel sequencing and bioinformatic methodologies to obtain an ancient mitogenome from an individual in the Beagle Channel. Phylogenetic analysis revealed a close relationship between this individual and Yamana and Chilean Antarctic individuals, suggesting a shared ancestor lived between 203 and 4,439 years ago. These findings support the hypothesis of reduced mobility in the Fuegian archipelago around 4500 years ago.
LATIN AMERICAN ANTIQUITY
(2023)
Article
Plant Sciences
Italo Antunes Pereira-Lima, Willian Batista-Silva, Joao Antonio Siqueira, Marcelle Ferreira Silva, David B. Medeiros, Joao Henrique Cavalcanti, Jose Francisco de Carvalho Gonsalves, Dimas Mendes Ribeiro, Alisdair R. Fernie, Adriano Nunes-Nesi, Wagner L. Araujo
Summary: Plants in acidic soils are negatively affected by aluminum, leading to impaired root growth and reduced plant yield. Organic acids can improve metal tolerance but at a metabolic cost. In this study, different ecotypes of Arabidopsis thaliana showed varying reductions in root growth and reproductive yield in response to aluminum stress. The expression of the ALMT1 gene, associated with aluminum resistance, was high in all ecotypes. Further analysis revealed accumulation of diverse amino acids, carbohydrates, and organic acids in shoots and roots of some ecotypes under aluminum stress. Our findings suggest that the capacity for using and translocating reduced carbon molecules is crucial for overcoming aluminum stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Luciana Zacour Del Giudice, Priscilla Falquetto-Gomes, Paulo Mafra de Almeida Costa, Auxiliadora O. Martins, Rebeca Patricia Omena-Garcia, Wagner L. Araujo, Agustin Zsogon, Edgard Augusto de Toledo Picoli, Adriano Nunes-Nesi
Summary: The development of fleshy fruits involves changes in size, mass, cell differentiation, anatomical and histological changes, and metabolic alterations. The regulation of metabolism and its association with anatomical changes in non-climacteric fruit development are poorly understood. In this study, three developmental phases of chili pepper were defined, each marked by distinct metabolic profiles. Mitochondrial activity was high in the early stages of development and declined over time, with a respiratory-like burst at the beginning of the ripening stage.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Jaciara Lana-Costa, Auxiliadora Oliveira Martins, Paulo Mafra de Almeida Costa, Mariela Mattos da Silva, Aline Delon Firmino, Diolina Moura Silva, Adriano Nunes-Nesi, Wagner L. Araujo
Summary: The rupture of the Funda similar to o dam in Mariana-MG, Brazil resulted in iron ore tailings that impacted the Doce River basin, affecting the flora and fauna in the region. Plants in contaminated soil showed changes in metabolism, reduced growth and biomass, and phytotoxicity symptoms. However, some species were able to exclude or accumulate metals through adaptive strategies. In this study, we investigated the physiological and metabolic responses in Restinga shrub species affected by the mining plume from the contaminated sea. We found that the rainy season led to the accumulation of metals and higher levels of these metals at the sampling stations. Increased stomatal conductance and intercellular CO2 concentration were observed during the rainy season, which was characterized by high temperatures. The results showed correlations between metals and metabolic responses, indicating acclimation responses such as maintenance of photosynthesis, and accumulation of free amino acids, carbohydrates, and antioxidants. The differential physiological responses between the two species analyzed suggest a genetic load capable of partially responding to stressful conditions associated with the iron ore tailing plume.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Jing Zhang, Peter G. L. Klinkhamer, Klaas Vrieling, T. Martijn Bezemer
Summary: This study found that Jacobaea vulgaris grew less well in live soil compared to sterilized soil, with the negative effects of soil inoculation on plant growth appearing to extend over the entire growth period but arising mainly from impacts on relative growth rates in the initial weeks.
JOURNAL OF PLANT ECOLOGY
(2022)