Detection and quantification of free sulfhydryls in monoclonal antibodies using maleimide labeling and mass spectrometry

Middle-up analysis of monoclonal antibodies after combined IgdE and IdeS hinge proteolysis: Investigation of free sulfhydryls

(2018) Valegh Faid et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Evaluation of disulfide scrambling during the enzymatic digestion of bevacizumab at various pH values using mass spectrometry

(2016) Wang-Chou Sung et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Beyond peptides and mAbs-current status and future perspectives for biotherapeutics with novel constructs

(2015) Dalal AlDeghaither et al.   JOURNAL OF CLINICAL PHARMACOLOGY

Long-Term Stabilization of Maleimide–Thiol Conjugates

(2014) Shaun D. Fontaine et al.   BIOCONJUGATE CHEMISTRY

Evaluation of Disulfide Bond Position to Enhance the Thermal Stability of a Highly Stable Single Domain Antibody

(2014) Dan Zabetakis et al.   PLoS One

The Identification of Free Cysteine Residues Within Antibodies a Potential Role for Free Cysteine Residues in Covalent Aggregation Because of Agitation Stress

(2013) Joon H. Huh et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression

(2013) Andrew Buchanan et al.   mAbs

Mass spectrometry-based strategies for protein disulfide bond identification

(2013) Pei Lun Tsai et al.   REVIEWS IN ANALYTICAL CHEMISTRY

Identification and Characterization of Buried Unpaired Cysteines in a Recombinant Monoclonal IgG1 Antibody

(2012) Taylor Zhang et al.   ANALYTICAL CHEMISTRY

Disulfide bond structures of IgG molecules

(2012) Hongcheng Liu et al.   mAbs

Tunable Degradation of Maleimide–Thiol Adducts in Reducing Environments

(2011) Aaron D. Baldwin et al.   BIOCONJUGATE CHEMISTRY

Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing

(2010) Melody Trexler-Schmidt et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mechanism of antibody reduction in cell culture production processes

(2010) Yung-Hsiang Kao et al.   BIOTECHNOLOGY AND BIOENGINEERING

Studies in serum support rapid formation of disulfide bond between unpaired cysteine residues in the VH domain of an immunoglobulin G1 molecule

(2009) D. Ouellette et al.   ANALYTICAL BIOCHEMISTRY

Localization and Quantitation of Free Sulfhydryl in Recombinant Monoclonal Antibodies by Differential Labeling with12C and13C Iodoacetic Acid and LC−MS Analysis

(2009) Tao Xiang et al.   ANALYTICAL CHEMISTRY

Identification and Localization of Unpaired Cysteine Residues in Monoclonal Antibodies by Fluorescence Labeling and Mass Spectrometry

(2009) Chris Chumsae et al.   ANALYTICAL CHEMISTRY

The Role of Thiols and Disulfides on Protein Stability

(2009) Maulik Trivedi et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Free sulfhydryl measurement as an indicator of antibody stability

(2008) Eilyn R. Lacy et al.   ANALYTICAL BIOCHEMISTRY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY