Article
Cell & Tissue Engineering
Ruiqiong Li, Haitong Zhang, Jinjin Zhang, Yunxia Ji, Wenbo Liu, Weili Liu, Meirong Wang, Changjun Lv, Xiaodong Song, Hongbo Li, Minge Li
Summary: This study aimed to investigate the effect of hucMSCs treatment on pulmonary fibrosis and explore the circFOXP1-mediated autophagic mechanism. Results showed that hucMSCs treatment alleviated pulmonary fibrosis by improving alveolar structure, reducing inflammation, and decreasing collagen deposition. The mechanistic study revealed that hucMSCs treatment downregulated circFOXP1 and promoted circFOXP1-mediated autophagy process, leading to a marked decrease in autophagy negative regulators EZH2, STAT1, and FOXK1. hucMSCs can be considered as an effective treatment for pulmonary fibrosis.
Article
Engineering, Biomedical
Daniel Levy, Sanaz Nourmohammadi Abadchi, Niloufar Shababi, Mohsen Rouhani Ravari, Nicholas H. Pirolli, Cade Bergeron, Angel Obiorah, Farzad Mokhtari-Esbuie, Shayan Gheshlaghi, John M. Abraham, Ian M. Smith, Emily H. Powsner, Talia J. Solomon, John W. Harmon, Steven M. Jay
Summary: Extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) have shown therapeutic potential, but their production is limited by donor-specific characteristics and decreased potency. Induced pluripotent stem cells (iPSCs) provide a scalable and reproducible source for obtaining differentiated MSCs (iMSCs) and their EVs demonstrate superior anti-inflammatory bioactivity. In a diabetic wound healing mouse model, iPSC EVs effectively mediate inflammation resolution, supporting the use of iPSCs as a source for therapeutic EV production.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Immunology
Xueping Li, Huaishuang Shen, Mao Zhang, Victoria Teissier, Ejun Elijah Huang, Qi Gao, Masanori Tsubosaka, Masakazu Toya, Junichi Kushioka, Chima V. Maduka, Christopher H. Contag, Simon Kwoon-Ho Chow, Ning Zhang, Stuart B. Goodman
Summary: This study revealed the dysfunctional bioenergetic activity of bone marrow-derived macrophages (Mφ) and mesenchymal stromal cells (MSCs) exposed to contaminated polyethylene particles (cPE), which may impair the immunoregulatory properties of cells in the bone niche. The underlying molecular defect related to disordered mitochondrial function could represent a potential therapeutic target during the resolution of inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Qiming Zhai, Xin Chen, Dongdong Fei, Xiaoyan Guo, Xiaoning He, Wanmin Zhao, Songtao Shi, John Justin Gooding, Fang Jin, Yan Jin, Bei Li
Summary: Chronic inflammation leads to mitochondrial dysfunction in tissue-specific mesenchymal stem cells (MSCs), but nanotechnology can be utilized to restore their function, providing an effective treatment for periodontitis and osteoarthritis.
Article
Cell Biology
Yuxin Wang, Qiaoling Song, Wei Huang, Yuxi Lin, Xin Wang, Chenyao Wang, Belinda Willard, Chenyang Zhao, Jing Nan, Elise Holvey-Bates, Zhuoya Wang, Derek Taylor, Jinbo Yang, George R. Stark
Summary: Type I interferons (IFN-I) play a crucial role in protecting against viral infections, with STAT2 being a key component in driving gene expression in response to IFN-I. The phosphorylation of STAT2 at T404 acts as a critical conformational switch, disrupting an inactive STAT1-STAT2 dimer and enhancing the antiviral defense response to IFN-I. Additionally, virus infection-induced activation of IKK-epsilon can directly phosphorylate T404, further facilitating the antiviral defense mechanism.
Article
Medicine, General & Internal
Yu Ning, Peisen Huang, Guihao Chen, Yuyan Xiong, Zhaoting Gong, Chunxiao Wu, Junyan Xu, Wenyang Jiang, Xiaosong Li, Ruijie Tang, Lili Zhang, Mengjin Hu, Jing Xu, Jun Xu, Haiyan Qian, Chen Jin, Yuejin Yang
Summary: This study found that MSCATV-EV, derived from ATV-treated MSCs, has a good cardiac repair effect on acute myocardial infarction. The results showed that MSCATV-EV alleviated inflammation in the infarct region by regulating macrophage polarization. Additionally, the study identified the miR-139-3p/Stat1 pathway as the mechanism underlying the promotion of macrophage polarization by MSCATV-EV.
Article
Cell Biology
Jingxian Yang, Janine Zara Bowring, Janes Krusche, Esther Lehmann, Tom Grunert, Benjamin Svejdal Bejder, Stephanie Fulaz Silva, Martin Saxtorph Bojer, Andreas Peschel, Hanne Ingmer
Summary: Bacteria use quorum sensing to coordinate group behavior, and some phages also respond to quorum sensing. These interactions likely impact bacterial group behavior, microbial ecology, and the efficacy of phage therapies.
Article
Biochemistry & Molecular Biology
Heng Wang, Wenqi Liang, Xuyang Wang, Yuchun Zhan, Wence Wang, Lin Yang, Yongwen Zhu
Summary: The study reveals that Notch signaling regulates glycolysis through the PI3K/Akt pathway to provide energy for embryonic growth. This study not only elucidates the important role of Notch signaling in embryonic development, but also provides new insights into the energy supply patterns during embryogenesis.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Multidisciplinary Sciences
Kathrin Koedderitzsch, Ekaterina Zezina, Lingzi Li, Matthias Herrmann, Nadine Biesemann
Summary: The study revealed a direct role of TNF in the glycolytic reprogramming of FLS, confirming the potency of immunometabolism in RA. TNF stimulation was shown to enhance glycolysis, reduce GLUT1 and GAPDH levels, which may be linked to treatment response in patients.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Declan Byrnes, Claire H. Masterson, Hector E. Gonzales, Sean D. McCarthy, Daniel P. O'Toole, John G. Laffey
Summary: Antimicrobial-resistant bacteria, such as Klebsiella species, are a common cause of hospital-acquired pneumonia, leading to high mortality and morbidity. Using mesenchymal stem cells (MSCs) to harness the host immune response to these bacteria shows promise in bypassing antimicrobial resistance mechanisms. This study tested the efficacy of repeated doses and preactivated MSCs in attenuating Klebsiella pneumonia-induced pneumonia in rat models. Two doses of MSCs were effective in improving lung function and reducing bacteria and injury, with preactivated MSCs showing superior results. MSC therapy also affected immune cell populations both locally and systemically. Repeated doses of MSCs, particularly preactivated MSCs, enhance their therapeutic potential in treating antimicrobial-resistant Klebsiella pneumonia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Yani He, Yuting Wang, Xiangbin Jia, Yingxue Li, Yao Yang, Lifei Pan, Rui Zhao, Yue Han, Feng Wang, Xiaoyue Guan, Tiezhou Hou
Summary: This study found that glycolysis plays an important role in regulating macrophage pyroptosis in bacterial infected diseases. The blockade of glycolysis can significantly suppress lipopolysaccharide-induced macrophage pyroptosis, resulting in a reduction of inflammation and bone resorption. The regulatory effect of glycolysis on macrophage pyroptosis may be mediated through the AMPK/SIRT1/NF-kappa B signaling pathway. These findings provide new insights into the role of glycolysis in periodontitis and offer a potential target for therapy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sepideh Saghati, cigir Biray Avci, Ayla Hassani, Sajed Nazifkerdar, Hassan Amini, Solmaz Saghebasl, Mahdi Mahdipour, Behnaz Banimohamad-Shotorbani, Atieh Rezaei Namjoo, Fateme Nazary Abrbekoh, Reza Rahbarghazi, Hamid Tayefi Nasrabadi, Ali Baradar Khoshfetrat
Summary: This study investigated the osteogenic properties of phenolated alginate hydrogel containing collagen and nano-hydroxyapatite on human mesenchymal stem cells. The results showed that the combination of alginate/collagen/nano-hydroxyapatite had suitable mechanical features and promoted cell viability, osteoblast differentiation, and new bone formation through involving the Wnt signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Xishuai Wang, Shiyu Zhao, Junhui Lai, Weijun Guan, Yang Gao
Summary: This study demonstrates that gingival-derived mesenchymal stem cells (GMSCs) intervene and protect against bleomycin-induced pulmonary fibrosis, while exhibiting anti-inflammatory and antioxidant capacities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Ok-Hyeon Kim, Jun-Hyung Park, Jong-In Son, Ok-Ja Yoon, Hyun-Jung Lee
Summary: The study demonstrates that silk fibroin scaffolds can enhance the anti-inflammatory and immunomodulatory functions of mesenchymal stromal cells, showing potential benefits for the treatment of inflammatory disorders.
Article
Immunology
Avinash Sanap, Supriya Kheur, Avinash Kharat, Ramesh Bhonde
Summary: This study investigates the effect of Interferon gamma (IFN-γ) and Ascorbic Acid (AA) preconditioning on the secretome of Umbilical Cord Derived MSCs (UCMSCs) and their potential to ameliorate the lipopolysaccharide (LPS) induced cytokine storm. The results demonstrate that IFN-γ and AA preconditioning of UCMSCs can alleviate the cytokine storm and enhance angiogenesis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Audiology & Speech-Language Pathology
Leonardo Wanderley Lopes, Allan Carlos Franca da Silva, Itacely Marinho da Silva, Maxsuel Alves Avelino de Paiva, Saulo Iordan do Nascimento Silva, Larissa Nadjara Alves Almeida, Vanessa Veis Ribeiro
Summary: This study verified the validity of internal consistency in the spectrographic analysis protocol (SAP). Participants evaluated and analyzed the relationship between items in the SAP. The results showed a good relationship between most items and the overall protocol. However, a few items had lower values, indicating a need for further training to properly understand these items.
Letter
Rheumatology
Martin Sebastian Winkler, Peter Korsten, Claudia Binder, Bjoern Tampe
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Oncology
Yi Liao, Senmao Li, Hao Chen, Chunyu Chen, Jintuan Huang, Feng Lin, Jianping Wang, Zuli Yang
Summary: A risk prediction system combining FIT and risk factors was developed to improve the sensitivity of colonoscopy screening. The system effectively stratified participants into high risk and low risk groups, with better predictive ability for colorectal neoplasia compared to using FIT alone.
EUROPEAN JOURNAL OF CANCER PREVENTION
(2023)
Article
Oncology
Luca Giraldi, Jovana Stojanovic, Dario Arzani, Roberto Persiani, Jinfu Hu, Kenneth C. Johnson, Zuo-Feng Zhang, Monica Ferraroni, Domenico Palli, Guo-Pei Yu, Carlo La Vecchia, Claudio Pelucchi, Nuno Lunet, Ana Ferro, Reza Malekzadeh, Joshua Muscat, David Zaridze, Dmitry Maximovich, Nuria Aragones, Vicente Martin, Jesus Vioque, Eva M. Navarrete-Munoz, Mohammadreza Pakseresht, Eva Negri, Matteo Rota, Farhad Pourfarzi, Lina Mu, Robert C. Kurtz, Areti Lagiou, Pagona Lagiou, Roberta Pastorino, Stefania Boccia
Summary: This study aimed to examine the association between height and risk of gastric cancer. Through a large pooled analysis of case-control studies, the study found no significant association between adult height and gastric cancer.
EUROPEAN JOURNAL OF CANCER PREVENTION
(2023)
Article
Oncology
Maria A. Karalexi, Marina Servitzoglou, Vassilios Papadakis, Denis Kachanov, Maja Cesen Mazic, Margaret Baka, Maria Moschovi, Maria Kourti, Sofia Polychronopoulou, Eftichia Stiakaki, Emmanuel Hatzipantelis, Helen Dana, Kalliopi Stefanaki, Astero Malama, Marios S. Themistocleous, Katerina Strantzia, Tatyana Shamanskaya, Panagiota Bouka, Paraskevi Panagopoulou, Maria Kantzanou, Evangelia Ntzani, Nick Dessypris, Eleni Th. Petridou
Summary: The prognosis of children with neuroblastoma varies depending on the stage and biology of the tumor, and early-stage neuroblastoma has a better prognosis. Treatment with anti-GD2 antibody can improve the prognosis of high-risk patients.
EUROPEAN JOURNAL OF CANCER PREVENTION
(2023)
Review
Clinical Neurology
Christoph Eckhard Heyde, Ulrich Josef Albert Spiegl, Anna Voelker, Nicolas von der Hoeh, Jeanette Henkelmann
Summary: The prevalence of nonspecific pyogenic spondylodiskitis has increased, causing high morbidity and mortality. The diagnosis is often delayed due to nonspecific clinical manifestations at the early stage. CT can assess the bony condition, while MRI is still the gold standard for diagnosis.
JOURNAL OF NEUROLOGICAL SURGERY PART A-CENTRAL EUROPEAN NEUROSURGERY
(2023)
Article
Pathology
Thomas Strecker, Felix Wiesmueller, Sabine Rudnik-Schoeneborn, Juliane Hoyer, Andre Reis, Michael Weyand, Abbas Agaimy
Summary: Aortic dissection is a life-threatening cardiovascular disease, and understanding the genetic causes, such as mutations of the ACTA2 gene, is crucial for identifying at-risk individuals and providing appropriate management and surveillance strategies.
Article
Clinical Neurology
Luis P. Kuschel, Juergen Hench, Stephan Frank, Ivana Bratic Hench, Elodie Girard, Maud Blanluet, Julien Masliah-Planchon, Martin Misch, Julia Onken, Marcus Czabanka, Dongsheng Yuan, Soeren Lukassen, Philipp Karau, Naveed Ishaque, Elisabeth G. Hain, Frank Heppner, Ahmed Idbaih, Nikolaus Behr, Christoph Harms, David Capper, Philipp Euskirchen
Summary: DNA methylation-based classification is important for the diagnosis of brain tumors. Nanopore sequencing provides a fast and cost-effective method for this classification.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2023)
Article
Rheumatology
Jasna Friscic, Christiane Reinwald, Martin Boettcher, Miranda Houtman, Maximilien Euler, Xi Chen, Kellie Walker, Philipp Kirchner, Honglin Zhu, Benjamin Wirth, Daniela Weidner, Rene Krueger, Vladimir Trajkovic, Arif B. B. Ekici, Kerstin Klein, Dimitrios Mougiakakos, Caroline Ospelt, Georg Schett, Markus H. H. Hoffmann
Summary: The induction of synovial fibroblasts plays a crucial role in the onset of arthritis flares, which is primarily mediated by epigenetic reprogramming. A BET inhibitor can target inflammatory tissue priming and reduce the severity of experimental arthritis in mice.
ARTHRITIS & RHEUMATOLOGY
(2023)
Article
Genetics & Heredity
Melissa Rieger, Sebastien Moutton, Sarah Verheyen, Katharina Steindl, Bernt Popp, Bruno Leheup, Celine Bonnet, Beatrice Oneda, Anita Rauch, Andre Reis, Mandy Krumbiegel, Ulrike Hueffmeier
Summary: Deletions in chromosome 19p13.11 were found in five individuals with neurodevelopmental disorders. They presented with psychomotor delay, behavioral and sleeping disorders, and various other malformations. These findings indicate an association of microdeletions at 19p13.11/19p13.11p12 with neurodevelopmental disorders, variable symptoms, and malformations.
EUROPEAN JOURNAL OF MEDICAL GENETICS
(2023)
Article
Hematology
F. Karl, C. Liang, R. Boettcher-Loschinski, A. Stoll, C. Flamann, S. Richter, C. Lischer, S. Voelkl, B. Jacobs, M. Boettcher, R. Jitschin, H. Bruns, T. Fischer, E. Holler, W. Roesler, T. Dandekar, A. Mackensen, D. Mougiakakos
Summary: Allo-SCT is a curative treatment for hematologic malignancies, relying on the graft-versus-leukemia (GVL) effect of donor T cells. Oxidative stress caused by DNA damage weakens the function of T cells and hampers the GVL effect. High levels of 8-hydroxydeoxyguanosine (8-OHdG), a biomarker for oxidative stress, were associated with enhanced T-cell activation, premature exhaustion, increased relapse risk, and shorter overall survival. Enhancing DNA repair in reconstituting T cells could improve GVL efficacy.
Letter
Oncology
Giusj Monia Pugliese, Veronica Venafra, Valeria Bica, Giorgia Massacci, Sara Latini, Simone Graziosi, Thomas Fischer, Dimitrios Mougiakakos, Martin Boettcher, Livia Perfetto, Francesca Sacco
Article
Oncology
Rebecca Baur, Franziska Karl, Romy Boettcher-Loschinski, Andrej Stoll, Simon Voelkl, Andreas Giessl, Cindy Flamann, Heiko Bruns, Ursula Schloetzer-Schrehardt, Martin Boettcher, Denis M. Schewe, Thomas Fischer, Regina Jitschin, Andreas Mackensen, Dimitrios Mougiakakos
Summary: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only curative treatment option for certain hematological malignancies. However, allo-HSCT can also lead to graft-versus-host disease (GvHD) and disease relapse. Recent studies have found that extracellular vesicles (EVs) expressing PD-L1 can suppress T-cell responses and contribute to GvHD and disease relapse in allo-HSCT patients.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Cell Biology
Foo Wei Ten, Dongsheng Yuan, Nabil Jabareen, Yin Jun Phua, Roland Eils, Soeren Lukassen, Christian Conrad
Summary: Feature identification and manual inspection are still important in single-cell sequencing data analysis. We propose using ensembles of autoencoders and rank aggregation to extract consistent features in a less biased manner. Our method can complement conventional tools and work with overlapping clustering identity assignment for transitional cell types or fates.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Andreas Mackensen, John B. A. G. Haanen, Christian Koenecke, Winfried Alsdorf, Eva Wagner-Drouet, Peter Borchmann, Daniel Heudobler, Barbara Ferstl, Sebastian Klobuch, Carsten Bokemeyer, Alexander Desuki, Florian Lueke, Nadine Kutsch, Fabian Mueller, Eveline Smit, Peter Hillemanns, Panagiotis Karagiannis, Erol Wiegert, Ying He, Thang Ho, Qing Kang-Fortner, Anna Melissa Schlitter, Catrine Schulz-Eying, Andrew Finlayson, Carina Flemmig, Klaus Kuehlcke, Liane Preussner, Benjamin Rengstl, Oezlem Tuereci, Ugur Sahin
Summary: This study evaluated the safety and efficacy of CAR-T cell therapy targeting Claudin 6 (CLDN6) in relapsed/refractory CLDN6-positive solid tumors. Manageable toxicity was observed, and objective response rate and disease control rate were assessed for the relevant tumors.