Journal
ANALYTICA CHIMICA ACTA
Volume 914, Issue -, Pages 53-61Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.02.015
Keywords
Microdialysis sampling; Raw fruits; Continuous flow system; Amperometric detection; Glucose enzymatic biosensor
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Funding
- Spanish Ministerio de Economia y Competitividad Research Project [CTQ2015-64402-C2-1-R]
- NANOAVANSENS Program from the Comunidad de Madrid [S2013/MT-3029]
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A non-destructive, rapid and simple to use sensing method for direct determination of glucose in non-processed fruits is described. The strategy involved on-line microdialysis sampling coupled with a continuous flow system with amperometric detection at an enzymatic biosensor. Apart from direct determination of glucose in fruit juices and blended fruits, this work describes for the first time the successful application of an enzymatic biosensor-based electrochemical approach to the non-invasive determination of glucose in raw fruits. The methodology correlates, through previous calibration set-up, the amperometric signal generated from glucose in non-processed fruits with its content in % (w/w). The comparison of the obtained results using the proposed approach in different fruits with those provided by other method involving the same commercial biosensor as amperometric detector in stirred solutions pointed out that there were no significant differences. Moreover, in comparison with other available methodologies, this microdialysis-coupled continuous flow system amperometric biosensor-based procedure features straightforward sample preparation, low cost, reduced assay time (sampling rate of 7 h(-1)) and ease of automation. (C) 2016 Elsevier B.V. All rights reserved.
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