Journal
JOURNAL OF TRANSLATIONAL MEDICINE
Volume 17, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12967-019-1825-3
Keywords
Extracellular vesicles; Liquid biopsy; Polyethylene glycol; ExoQuick((R)); PureExo((R)); Ultracentrifugation
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Funding
- 'Fondo de Investigaciones Sanitarias' (FIS) [PI14_00077]
- Miguel Servet Program del Instituto de Salud Carlos III [CP11/00147]
- Ministerio de Economia y Competitividad-FEDERER [RTC-2016-4990-1, RTC-2015-3846-1]
- FPI-CEU predoctoral fellowship
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BackgroundExtracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, and it might provide an alternative to traditional solid biopsies. However, the most effective approach for EV isolation is not yet wellestablished.ResultsHere, we study the efficiency of the most common EV isolation methods-ultracentrifugation, Polyethlyene glycol and two commercial kits, Exoquick((R)) and PureExo((R)). We isolated circulating EVs from the bloodstream of healthy donors, characterized the size and yield of EVs and analyzed their protein profiles and concentration. Moreover, we have used for the first time Digital-PCR to identify and detect specific gDNA sequences, which has several implications for diagnostic and monitoring many types of diseases.ConclusionsOur findings present Polyethylene glycol precipitation as the most feasible and less cost-consuming EV isolation technique.
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