Journal
ANALYST
Volume 141, Issue 11, Pages 3395-3402Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6an00473c
Keywords
-
Categories
Funding
- National Key Scientific Program of China [2011CB911000]
- National Key Basic Research Program of China [2013CB932702]
- NSFC [21325520, 21327009, J1210040, 21177036]
- Foundation for Innovative Research Groups of NSFC [21221003, 21521063]
- National Instrumentation Program [2011YQ030124]
- Hunan Provincial Collaborative Innovation Center for Chemistry and Molecular Medicine
- Hunan Provincial Natural Science Foundation [11JJ1002]
Ask authors/readers for more resources
Formaldehyde (FA) plays an important role in living systems as a reactive carbonyl species (RCS). An abnormal degree of FA is known to induce neurodegeneration, cognitive decrease and memory loss owing to the formation of strong cross-link DNA and protein and other molecules. The development of efficient methods for biological FA detection is of great biomedical importance. Although a few one-photon FA fluorescent probes have been reported for imaging in living cells, probes excited by two photons are more suitable for bio-imaging due to their low background fluorescence, less photobleaching, and deep penetration depth. In this study, a two-photon fluorescent probe FATP1 for FA detection and bio-imaging in living cells and tissues was reported. The detection is based on the 2-aza-Cope sigmatropic rearrangement followed by elimination to release the fluorophore, resulting in both one-and two-photon excited fluorescence increase. The probe FATP1 showed a high sensitivity to FA with a detection limit of 0.2 mu M. Moreover, FATP1 enabled the two-photon bio-imaging of FA in live HEK-293 cells and tissues with tissue-imaging depths of 40-170 mu m. Furthermore, FATP1 could be applied for the monitoring of endogenous FA in live MCF-7 cells, presaging its practical applications in biological systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available