Article
Virology
Francisco Dominguez, Oksana Palchevska, Elena I. Frolova, Ilya Frolov
Summary: Alphavirus replicons are efficient tools for expressing heterologous genetic information, but their expression levels are cell-specific and dependent on the origin of the replicons. They mimic alphavirus infections and induce immune responses, which need to be carefully examined. However, replicons can also be manipulated to enhance protein expression and interactions with host cells.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemical Research Methods
Inga Tijunelyte, Jeffrey Teillet, Paul Bruand, Remi Courson, Aurelie Lecestre, Pierre Joseph, Aurelien Bancaud
Summary: This study optimized a fluorimetry-based DNA detection technology by controlling the concentration of linear targets and probes. It was found that using specific lengths of target and probe, as well as chips fabricated with grayscale lithography, significantly enhanced sensitivity and detection range while maintaining a short detection time.
Article
Chemistry, Analytical
Gaurav Pendharkar, Yen-Ta Lu, Chia-Ming Chang, Meng-Ping Lu, Cheng-Hsien Liu
Summary: This study introduces an insulator-based dielectrophoretic microfluidic lab-chip that can precisely pair cells and achieve cell fusion with high efficiency. The chip demonstrates high cell fusion yields and maintains cell viability, making it a promising tool for cell fusion research.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Taekeon Jung, Young-Ran Yun, Jaehyun Bae, Sung Yang
Summary: By integrating magnetic particle conjugation for bacteria concentration and separation, a new impedimetric bacteria-detection sensor has been developed for rapid and accurate detection of low bacteria concentrations in food samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Virology
Feixiang Yan, Wei Yang, Xinlu Wang, Guangxia Gao
Summary: This study found that the ISG product TMEM45B inhibits the replication of Sindbis virus (SINV). TMEM45B interacts with the viral nonstructural proteins Nsp1 and Nsp4, inhibiting the translation and promoting the degradation of SINV RNA. In addition, TMEM45B also interacts with Nsp1 and Nsp4 of chikungunya virus (CHIKV), suggesting its potential role in inhibiting the replication of other alphaviruses.
JOURNAL OF VIROLOGY
(2022)
Article
Chemistry, Analytical
Ryu Nakabayashi, Masanori Eguchi
Summary: This paper proposes a modified iDEP device for single-cell analysis. The device is capable of measuring particle velocity at a wide frequency range and produces consistent results.
Article
Mechanics
Raul Fernandez-Mateo, Hywel Morgan, Antonio Ramos, Pablo Garcia-Sanchez
Summary: We experimentally investigate the repulsion between charged microscopic particles and the channel walls during electrophoresis in microfluidic devices. This repulsion is primarily caused by hydrodynamic interactions arising from flow vortices induced by the slip velocity on the particle surface due to the electric field. Measurement of wall-particle separation allows us to infer the maximum slip velocity on the particle surface, including very small velocities that are otherwise difficult to directly measure. The data at low electric field amplitudes agree with theoretical predictions based on Concentration Polarization Electro-osmosis (CPEO), but the predictions of CPEO theory break down for higher electric fields. Furthermore, we show that strong electric fields can disrupt the quadrupolar flow structures, leading to a weaker wall repulsion.
Article
Materials Science, Multidisciplinary
Geon-Tae Park, Hoon-Hee Ryu, Tae-Chong Noh, Gyeong-Cheol Kang, Yang-Kook Sun
Summary: In this study, the effect of calcination temperature on the physicochemical properties and electrochemical performance of full-concentration-gradient Li[Ni0.78Co0.10Mn0.12]O-2 (FCG NCM78) was comprehensively investigated. The results showed that the electrochemical performance of FCG NCM78 was significantly influenced by its physical properties, such as crystallinity, compositional gradient, and morphology. FCG NCM78 calcined at the optimal temperature retained its morphological texture and exhibited high crystallinity, resulting in remarkable cycling stability and superior rate capability. On the other hand, excessively coarsened FCG NCM78 cathode particles, obtained at high calcination temperatures, developed permanent microcracks, leading to severe structural damage and rapid deterioration of the solid electrolyte interphase layer.
Article
Immunology
Thomas Tipih, Mark Heise, Felicity Jane Burt
Summary: This study demonstrated the expression of CCHFV nucleoprotein by a Sindbis virus vector and its immunogenicity in mice. The induced immune response, predominantly Th1-type, was shown by the spectrum of cytokine production and antibody profile. Further studies in CCHFV-susceptible animals are needed to evaluate the protective efficacy of the vaccine.
Article
Biochemistry & Molecular Biology
Jia-Qi Li, Zhe-Rui Zhang, Hong-Qing Zhang, Ya-Nan Zhang, Xiang-Yue Zeng, Qiu-Yan Zhang, Cheng-Lin Deng, Xiao-Dan Li, Bo Zhang, Han-Qing Ye
Summary: The study demonstrated a safe and cost-effective method to prevent SARS-CoV-2 infection by expressing neutralizing antibodies in the lungs. Through in vitro and in vivo assays, it was shown that self-replicating mRNA could efficiently block SARS-CoV-2 infection with reduced viral titer and tissue damage in the lung with the help of lung target delivery using the VRP system.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Chemistry, Analytical
Olga Naumova, Vladimir Generalov, Dmitry Shcherbakov, Elza Zaitseva, Yuriy Zhivodkov, Anton Kozhukhov, Alexander Latyshev, Alexander Aseev, Alexander Safatov, Galina Buryak, Anastasia Cheremiskina, Julia Merkuleva, Nadezhda Rudometova
Summary: Quick label-free virus screening and highly sensitive analytical tools/techniques are crucial in a pandemic. In this study, a biosensing device based on silicon nanoribbon sensors was developed for the detection and characterization of viruses and their protein components. The sensors demonstrated subattomolar sensitivity and a dynamic range of 4 orders, with the ability to distinguish between specific and nonspecific interactions. Dielectrophoretic controlled sensors allowed for estimating the effective charge of individual viruses at the sensor surface, separate from the charge associated with protein binding.
Article
Biochemical Research Methods
Mariia Kepper, Md Nurul Karim, Michael Baune, Jorg Thoeming, Georg R. Pesch
Summary: Recovering noble materials from waste is crucial for industries worldwide. Dielectrophoretic (DEP) filtration, an electrically switchable particle separation technique, is a promising approach to address this challenge. Improving the efficiency and high throughput capability of DEP filtration is necessary to make it a viable alternative to conventional separation methods.
Article
Virology
Isabel Fourie, Jumari Snyman, June Williams, Arshad Ismail, Petrus Jansen van Vuren, Marietjie Venter
Summary: This study investigated the pathogenesis and clinical presentation of MIDV and SINV infections in horses in South Africa. MIDV infection was more prevalent than SINV infection, and MIDV cases showed a seasonal pattern while SINV cases did not. The study also found prolonged replication of MIDV compared to SINV.
Article
Chemistry, Analytical
Mohammad K. D. Manshadi, Amin Mansoorifar, Jung-Chih Chiao, Ali Beskok
Summary: This study introduces inexpensive, rapid, sensitive, and quantifiable impedance-based immunosensors to evaluate the concentration of neutralizing antibodies (NAb) against the coronavirus. The sensor detection mechanism is mainly based on the diffusion of biomolecules onto the electrode surface. The results demonstrate that the sensor is capable of detecting NAb in the range of 45 ng/mL to 185 ng/mL. Due to its fast test results and lower costs, this sensor can be used to assess NAb in people's blood serum before receiving further COVID vaccine doses.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Moheb Amir Mahani, Ahmad Naseri Karimvand, Naser Naserifar
Summary: This work combines multiorifice fluid fractionation with dielectrophoresis-based actuator to create a new device for size-based particle separation. The addition of the actuator improves the performance and expands the range of flow rates for separation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Ahmad Hosseinizadeh, Ghoncheh Mashayekhi, Jeremy Copperman, Peter Schwander, Ali Dashti, Reyhaneh Sepehr, Russell Fung, Marius Schmidt, Chun Hong Yoon, Brenda G. Hogue, Garth J. Williams, Andrew Aquila, Abbas Ourmazd
Article
Chemistry, Multidisciplinary
Xuanxuan Li, John C. H. Spence, Brenda G. Hogue, Haiguang Liu
Article
Multidisciplinary Sciences
Xuanxuan Li, Chun-Ya Chiu, Hsiang-Ju Wang, Stephan Kassemeyer, Sabine Botha, Robert L. Shoeman, Robert M. Lawrence, Christopher Kupitz, Richard Kirian, Daniel James, Dingjie Wang, Garrett Nelson, Marc Messerschmidt, Sebastien Boutet, Garth J. Williams, Elisabeth Hartmann, Aliakbar Jafarpour, Lutz M. Foucar, Anton Barty, Henry Chapman, Mengning Liang, Andreas Menzel, Fenglin Wang, Shibom Basu, Raimund Fromme, R. Bruce Doak, Petra Fromme, Uwe Weierstall, Michael H. Huang, John C. H. Spence, Ilme Schlichting, Brenda G. Hogue, Haiguang Liu
Article
Multidisciplinary Sciences
Hemanth K. N. Reddy, Chun Hong Yoon, Andrew Aquila, Salah Awel, Kartik Ayyer, Anton Barty, Peter Berntsen, Johan Bielecki, Sergey Bobkov, Maximilian Bucher, Gabriella A. Carini, Sebastian Carron, Henry Chapman, Benedikt Daurer, Hasan DeMirci, Tomas Ekeberg, Petra Fromme, Janos Hajdu, Max Felix Hanke, Philip Hart, Brenda G. Hogue, Ahmad Hosseinizadeh, Yoonhee Kim, Richard A. Kirian, Ruslan P. Kurta, Daniel S. D. Larsson, N. Duane Loh, Filipe R. N. C. Maia, Adrian P. Mancuso, Kerstin Muhlig, Anna Munke, Daewoong Nam, Carl Nettelblad, Abbas Ourmazd, Max Rose, Peter Schwander, Marvin Seibert, Jonas A. Sellberg, Changyong Song, John C. H. Spence, Martin Svenda, Gijs Van der Schot, Ivan A. Vartanyants, Garth J. Williams, P. Lourdu Xavier
Article
Multidisciplinary Sciences
Dan Bi Lee, Jong-Min Kim, Jong Hyeon Seok, Ji-Hye Lee, Jae Deok Jo, Ji Young Mun, Chelsie Conrad, Jesse Coe, Gerrett Nelson, Brenda Hogue, Thomas A. White, Nadia Zatsepin, Uwe Weierstall, Anton Barty, Henry Chapman, Petra Fromme, John Spence, Mi Sook Chung, Chang-Hyun Oh, Kyung Hyun Kim
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Arpan Deb, William A. Johnson, Alexander P. Kline, Boston J. Scott, Lydia R. Meador, Dustin Srinivas, Jose M. Martin-Garcia, Katerina Dorner, Chad R. Borges, Rajeev Misra, Brenda G. Hogue, Petra Fromme, Tsafrir S. Mor
Article
Chemistry, Multidisciplinary
Max Rose, Sergey Bobkov, Kartik Ayyer, Ruslan P. Kurta, Dmitry Dzhigaev, Young Yong Kim, Andrew J. Morgan, Chun Hong Yoon, Daniel Westphal, Johan Bielecki, Jonas A. Sellberg, Garth Williams, Filipe R. N. C. Maia, Olexander M. Yefanov, Vyacheslav Ilyin, Adrian P. Mancuso, Henry N. Chapman, Brenda G. Hogue, Andrew Aquila, Anton Barty, Ivan A. Vartanyants
Article
Chemistry, Multidisciplinary
Max O. Wiedorn, Salah Awel, Andrew J. Morgan, Kartik Ayyer, Yaroslav Gevorkov, Holger Fleckenstein, Nils Roth, Luigi Adriano, Richard Bean, Kenneth R. Beyerlein, Joe Chen, Jesse Coe, Francisco Cruz-Mazo, Tomas Ekeberg, Rita Graceffa, Michael Heymann, Daniel A. Horke, Juraj Knoska, Valerio Mariani, Reza Nazari, Dominik Oberthuer, Amit K. Samanta, Raymond G. Sierra, Claudiu A. Stan, Oleksandr Yefanov, Dimitrios Rompotis, Jonathan Correa, Benjamin Erk, Rolf Treusch, Joachim Schulz, Brenda G. Hogue, Alfonso M. Ganan-Calvo, Petra Fromme, Jochen Kuepper, Andrei V. Rode, Sasa Bajt, Richard A. Kirian, Henry N. Chapman
Article
Chemistry, Multidisciplinary
Yingchen Shi, Ke Yin, Xuecheng Tai, Hasan DeMirci, Ahmad Hosseinizadeh, Brenda G. Hogue, Haoyuan Li, Abbas Ourmazd, Peter Schwander, Ivan A. Vartanyants, Chun Hong Yoon, Andrew Aquila, Haiguang Liu
Article
Optics
Kartik Ayyer, Andrew J. Morgan, Andrew Aquila, Hasan DeMirci, Brenda G. Hogue, Richard A. Kirian, P. Lourdu Xavier, Chun Hong Yoon, Henry N. Chapman, Anton Barty
Letter
Virology
LaRinda A. Holland, Emily A. Kaelin, Rabia Maqsood, Bereket Estifanos, Lily I. Wu, Arvind Varsani, Rolf U. Halden, Brenda G. Hogue, Matthew Scotch, Efrem S. Lima
JOURNAL OF VIROLOGY
(2020)
Article
Physics, Multidisciplinary
Egor Sobolev, Serguey Zolotarev, Klaus Giewekemeyer, Johan Bielecki, Kenta Okamoto, Hemanth K. N. Reddy, Jakob Andreasson, Kartik Ayyer, Imrich Barak, Sadia Bari, Anton Barty, Richard Bean, Sergey Bobkov, Henry N. Chapman, Grzegorz Chojnowski, Benedikt J. Daurer, Katerina Doerner, Tomas Ekeberg, Leonie Fluckiger, Oxana Galzitskaya, Luca Gelisio, Steffen Hauf, Brenda G. Hogue, Daniel A. Horke, Ahmad Hosseinizadeh, Vyacheslav Ilyin, Chulho Jung, Chan Kim, Yoonhee Kim, Richard A. Kirian, Henry Kirkwood, Olena Kulyk, Jochen Kuepper, Romain Letrun, N. Duane Loh, Kristina Lorenzen, Marc Messerschmidt, Kerstin Muhlig, Abbas Ourmazd, Natascha Raab, Andrei Rode, Max Rose, Adam Round, Takushi Sato, Robin Schubert, Peter Schwander, Jonas A. Sellberg, Marcin Sikorski, Alessandro Silenzi, Changyong Song, John C. H. Spence, Stephan Stern, Jolanta Sztuk-Dambietz, Anthon Teslyuk, Nicusor Timneanu, Martin Trebbin, Charlotte Uetrecht, Britta Weinhausen, Garth J. Williams, P. Lourdu Xavier, Chen Xu, Ivan A. Vartanyants, Victor S. Lamzin, Adrian Mancuso, Filipe R. N. C. Maia
COMMUNICATIONS PHYSICS
(2020)
Article
Immunology
Karen V. Kibler, Mateusz Szczerba, Douglas Lake, Alexa J. Roeder, Masmudur Rahman, Brenda G. Hogue, Lok-Yin Roy Wong, Stanley Perlman, Yize Li, Bertram L. Jacobs
Summary: The heavily mutated spike protein of the Omicron SARS-CoV-2 variant raises concerns due to potential antibody escape. However, a study showed that intranasal or scarification immunization with a replication-competent poxvirus expressing the pre-fusion stabilized spike protein of the Washington strain fully protected mice against severe disease and death caused by the heavily mutated mouse-adapted SARS2-N501Y (MA30) strain.
Article
Chemistry, Analytical
Paul V. Jones, Gabriel L. Salmon, Alexandra Ros
ANALYTICAL CHEMISTRY
(2017)
Meeting Abstract
Chemistry, Multidisciplinary
Mark Hayes, Claire Crowther, Shannon Hilton, Paul Jones
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2016)
