Journal
ANALYST
Volume 141, Issue 11, Pages 3251-3258Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6an00350h
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Funding
- Canada Research Chairs program in Photonics and Nanoscience
- Canada Research Chairs program in Molecular Neurobiology
- NSERC Discovery Grant program
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Tip-enhanced Raman spectroscopy (TERS) provides greatly enhanced Raman signals along with ultra-high lateral spatial resolutions and has been demonstrated to be a technique of choice to study a variety of biochemical specimens such as DNA and RNA at the single chain level. However, the sensitivity of TERS to demonstrate the influence of the nanoscale environment on DNA properties has not been investigated. Herein, we used a gap-mode TERS as an ultra-sensitive label-free technique to investigate the influence of the local plasmid on the DNA properties of a beta(2)-adrenergic receptor (beta(2)AR). Remarkable lateral spatial resolutions down to 8 nm were also acquired for the collected Raman signals under ambient conditions. This approach offers not only a tool to examine the influence of the local nanoscale environment surrounding the DNA structure, but also the localization of the majority of nucleic acid base (s) present in selected regions on the DNA strand.
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