Article
Chemistry, Analytical
Brian C. Hauck, Charles S. Harden, Vincent M. McHugh
Summary: The search for suitable ion mobility spectrometry (IMS) calibrant compounds continues, with a focus on accurately measuring reduced ion mobility (K-0) values under various instrumental conditions. Isoflurane (IsoF) is highlighted as a strong candidate for negative ion mode calibrant, and its accurately measured K-0 values are discussed in this report. The evaluation of IsoF as a negative ion mode calibrant for IMS applications is the main focus of this study.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Analytical
Ines C. Santos, Jennifer S. Brodbelt
Summary: The development of new strategies for analyzing nucleic acids has gained momentum due to increased interest in using these biomolecules as drugs or drug targets. Application of new mass spectrometry ion activation techniques and optimization of separation methods have allowed more detailed characterization of nucleic acids and oligonucleotide therapeutics, leading to confirmation of sequence, localization of modifications and interaction sites, structural analysis, and identification of failed sequences and degradation products.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Chemistry, Analytical
Daniel G. Delafield, Gaoyuan Lu, Cameron J. Kaminsky, Lingjun Li
Summary: Mass spectrometry-based biomolecular analyses have become popular in research settings. Gas phase ion separation techniques provide a fourth dimension of analysis, enabling rapid characterization and high throughput profiling. This review highlights the latest developments and applications of high-end ion-mobility enabled instruments and their advantages for biomolecular research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Taylor M. Domenick, Austin L. Jones, Robin H. J. Kemperman, Richard A. Yost
Summary: To address the poor retention of highly polar metabolites in reversed-phase liquid chromatography (RPLC), a derivatization agent and an internal standard were introduced to improve analyte retention and detection limits. Multiplexed drift-tube ion mobility (IM) was integrated into the LC-MS workflow to enhance method sensitivity and selectivity. The results showed that this method allowed for the quantitation of amino acids in microglial cell extracts.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Juraj Konc, Libby Brown, Daniel R. Whiten, Yukun Zuo, Peter Ravn, David Klenerman, Goncalo J. L. Bernardes
Summary: A new bioconjugation platform has been developed for the preparation of DNA-antibody bioconjugates using a simple reagent, resulting in improved homogeneity of the prepared conjugates suitable for fluorescence or super-resolution microscopy cell imaging experiments.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Chia-Hsin Chi, Yi-Sheng Wang, Lean-Teik Ng
Summary: A novel method for the characterization of linkages and anomeric configuration of polysaccharides was proposed using ion mobility-mass spectrometry (IM-MS). The method was validated and successfully applied to analyze polysaccharides from medicinal mushrooms.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Shutong Yang, Liancheng Gu, Fangling Wu, Xinhua Dai, Fuxing Xu, Qiaoyu Li, Xiang Fang, Shaoning Yu, Chuan-Fan Ding
Summary: This study developed an effective strategy for enantiomeric determination of amino acids, which can easily distinguish all 19 natural amino acid enantiomers. The role of Mg2+ in host/guest interactions was also demonstrated.
Article
Biochemical Research Methods
Christian Manz, Michael Goetze, Clemens Frank, Andreas Zappe, Kevin Pagel
Summary: LC-MS is an important tool for the comprehensive characterization of N-glycans. However, the measurement times of HILIC chromatography remain a bottleneck in glycan analysis. This study demonstrates the use of an internal calibration strategy to speed up glycan analysis by reducing the number of utilized calibrant. The minimized calibrant shows accurate calibration and can be applied in HPLC runs without modification.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Rebecca Beveridge, Antonio N. Calabrese
Summary: Intrinsically disordered proteins and regions of intrinsic disorder play crucial roles in biological processes by overcoming binding restrictions and enhancing binding specificity. Structural mass spectrometry methods are increasingly important for studying these proteins, providing new insights into their structure and function. Their flexibility and advantages make them ideal tools for understanding intrinsically disordered proteins.
FRONTIERS IN CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Lukasz Polewski, Andreas Springer, Kevin Pagel, Christoph A. Schalley
Summary: Ion mobility spectrometry and gas-phase IR action spectroscopy are two popular structure-sensitive mass-spectrometric methods for investigating supramolecular assemblies. These methods can distinguish isomers, reveal subtle structural details, and study amino acid complexes and peptide assemblies.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
David S. Roberts, Morgan Mann, Jake A. Melby, Eli J. Larson, Yanlong Zhu, Allan R. Brasier, Song Jin, Ying Ge
Summary: Researchers have successfully elucidated the complete structure of newly discovered O-glycans on the S protein regional-binding domain (SR-BD) of the SARS-CoV-2 virus through a hybrid mass spectrometry approach. This method can provide insights into the interactions between the virus and host cells, laying a foundation for understanding the functional roles of O-glycans in SARS-CoV-2 and other glycoproteins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Chemistry, Analytical
Thamina Acter, Nizam Uddin, Eunji Cho, Eleanor Riches, Pierre Giustie, Carlos Afonso, Sunghwan Kim
Summary: Understanding the chemical composition and characteristics of petroleum requires molecular-level characterization and structural identification. Mass spectrometry techniques, especially ultrahigh-resolution mass spectrometry and its coupling with other complementary tools, are important analytical methods. Ion mobility coupled with high-resolution mass spectrometry has proven to be valuable in structural characterization of petroleum samples. This review provides instructive examples and future perspectives of the application of ion mobility mass spectrometry in petroleum analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Diana C. Velosa, Andrew J. Dunham, Marcus E. Rivera, Shon P. Neal, Christopher D. Chouinard
Summary: This study utilizes ion mobility technology to improve the analysis resolution and structural elucidation of steroids through structurally selective reactions. The results demonstrate that derivatization of hydroxyl stereoisomer pairs can significantly increase resolution, and different products can be obtained through derivatization of the carbonyl group. These changes can be easily interpreted using parameters such as accurate mass and collision cross section.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Review
Chemistry, Analytical
Yuelin Song, Qingqing Song, Wenjing Liu, Jun Li, Pengfei Tu
Summary: Currently, MS/MS is widely used in metabolomics for the identification of metabolites. However, the structural identification of isomers remains challenging due to the similarity of their MS/MS spectra. Differentiation and identification of isomers using MS/MS can be facilitated by various techniques, including chemical derivatization, ion mobility spectrometry, ion dissociation techniques, and energy-resolved MS. This tutorial provides a comprehensive summary of these cutting-edge techniques and emphasizes the importance of isomeric identification in metabolomics for analytical chemists. (c) 2023 Elsevier B.V. All rights reserved.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)