Article
Plant Sciences
K. Trush, A. Eliasova, M. D. Monje-Rueda, V. Kolarcik, M. Betti, P. Pal'ove-Balang
Summary: Leaves of Lotus japonicus infected by powdery mildew accumulate isoflavonoids, especially vestitol and sativan as protective compounds. The application of methyl jasmonate showed a moderate effect on the accumulation of vestitol, while chitosan application led to a strong increase in vestitol content. Furthermore, chitosan also induced the expression of key enzymes involved in vestitol and sativan biosynthesis.
BIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Yasuhide Hiraga, Norimoto Shimada, Yoshiki Nagashima, Kunihiro Suda, Tina Kanamori, Kanako Ishiguro, Yuka Sato, Hideki Hirakawa, Shusei Sato, Tomoyoshi Akashi, Yoshikazu Tanaka, Daisaku Ohta, Koh Aoki, Daisuke Shibata, Hideyuki Suzuki, Kota Kera
Summary: The study identified LjF8H as the enzyme responsible for gossypetin biosynthesis in Lotus japonicus, showing broad substrate acceptance and the impact of substrate conformation on product specificity. Overexpression of LjF8H in plant cells demonstrated its functionality in synthesizing specific flavonol derivatives.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Maria Kaducova, Adriana Eliasova, Kristina Trush, Michaela Bacovcinova, Katarina Sklenkova, Peter Palove-Balang
Summary: UV-B irradiation induced changes in the concentration of flavonoid compounds in Lotus corniculatus, affecting the expression of key enzymes and leading to accumulation of certain isoflavonoids.
THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiao Liu, Leru Liu, Lu Tian, Shaoming Xu, Guojiang Wu, Huawu Jiang, Yaping Chen
Summary: In this study, the expression characteristics and potential functions of the polyol transporter LjPLT3 in Lotus japonicus under salt stress were investigated. LjPLT3 was found to be expressed in the vascular tissue of leaves, stems, roots, and nodules, and its expression was induced by NaCl treatment. Overexpression of LjPLT3 in L. japonicus resulted in modified growth rate and saline tolerance of the transgenic plants. Transgenic seedlings exhibited higher chlorophyll concentration, fresh weight, and survival rate after salt treatment compared to the wild type.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Jianhua Wang, Mingwei Li, Jinlin Feng, Xiaoyan Yan, Huize Chen, Rong Han
Summary: The study showed that pretreatment with TiO2-NPs can alleviate UV-B stress on plants by promoting flavonoid synthesis, improving the antioxidant system, and preventing depolymerization of microtubules, with a safe and effective application dose of 10 mg/L.
Article
Biotechnology & Applied Microbiology
Daniela Tsikou, Christina N. Nikolaou, Myrto Tsiknia, Kalliope K. Papadopoulou, Constantinos Ehaliotis
Summary: This study identifies the main factors influencing the tripartite association between legumes, arbuscular mycorrhiza fungi (AMF), and nitrogen-fixing rhizobia. It finds that different AMF strains can enhance nodulation in legumes, especially under heat-stress conditions. The impact of rhizobia on mycorrhizal colonization depends on the specific AMF strain. Additionally, phosphorus enhances nodulation while nitrogen does not affect mycorrhizal colonization.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mingchao Huang, Mengru Yuan, Chunyu Sun, Meiru Li, Pingzhi Wu, Huawu Jiang, Guojiang Wu, Yaping Chen
Summary: Arabidopsis AGD2 and its homolog ALD1 have divergent roles in plant defense. The study reveals that LjAGD2a plays important roles in the interaction between legume plants and microbes, affecting plant development and resistance to pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Jingyao Xu, Tingyu Shan, Jingjing Zhang, Xinxin Zhong, Yijia Tao, Jiawen Wu
Summary: In this study, a full-length transcriptome database of R. japonicus was constructed using PacBio iso-seq and DNB-seq technologies. The analysis identified 29 differentially expressed genes encoding nine key enzymes involved in flavonoid biosynthesis. Flavanone 3-hydroxylase and flavonol synthase genes were found to play important roles in flavonoid accumulation in different tissues of R. japonicus. Additionally, 22 MYB transcription factors associated with flavonoid and phenylpropanoid biosynthesis were discovered. These results provide genetic information and insights into the molecular mechanism of flavonoid biosynthesis in R. japonicus, and also lay a foundation for the development of its medicinal value.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Multidisciplinary Sciences
Jieshun Lin, Yuda Purwana Roswanjaya, Wouter Kohlen, Jens Stougaard, Dugald Reid
Summary: Legumes balance nitrate signaling and symbiotic nitrogen fixation through cytokinin biosynthesis, with low nitrate conditions promoting cytokinin induction and high nitrate inhibiting it. This differential response is further seen in mutants, which show hypersensitivity to nitrate inhibition but can be rescued by cytokinin application. Nitrate's inhibitory effect on symbiosis occurs in a Nlp1 and Nlp4 dependent manner, contrasting with its positive influence on non-legumes.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Jesus Montiel, Dugald Reid, Thomas H. Gronbaek, Caroline M. Benfeldt, Euan K. James, Thomas Ott, Franck A. Ditengou, Marcin Nadzieja, Simon Kelly, Jens Stougaard
Summary: This study reports intercellular infection of the model legume Lotus japonicus by the IRBG74 strain and reveals the genetic requirements and differences in the pathways governing this process compared to intracellular infection. The results indicate a differential requirement for cytokinin signaling during intercellular rhizobial entry, highlighting distinct modalities of inter- and intracellular infection mechanisms in L. japonicus.
Review
Agriculture, Multidisciplinary
Josef L. Ranner, Sabrina Schalk, Cindy Martyniak, Martin Parniske, Caroline Gutjahr, Timo D. Stark, Corinna Dawid
Summary: This article provides an overview of the primary and secondary metabolites identified in Lotus japonicus to date. More than 600 metabolites are grouped into compound classes such as polyphenols, carbohydrates, organic acids and phosphates, lipids, amino acids, nitrogenous compounds, phytohormones, and additional defense compounds. Their physiological effects are briefly discussed, and the detection methods are explained. This review of the exisiting literature on Lotus japonicus metabolites provides a valuable basis for future metabolomics studies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Yongpeng Li, Wei Qin, Xueqing Fu, Yaojie Zhang, Danial Hassani, Sadaf-Ilyas Kayani, Lihui Xie, Hang Liu, Tiantian Chen, Xin Yan, Bowen Peng, Kuanyu Wu-Zhang, Chen Wang, Xiaofen Sun, Ling Li, Kexuan Tang
Summary: UV-B radiation plays a pivotal role in regulating plant growth and metabolite biosynthesis. In Artemisia annua, the transcriptional responses to UV-B radiation revealed potential involvement of specific TFs, such as AaMYB6 and AaMYB4, in the regulation of artemisinin and flavonoid biosynthesis pathways.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Agronomy
Meiru Li, Hongqing Li
Summary: Manipulation of floral transition allows plants to adjust their growth period and avoid adverse environmental conditions. The study investigated the effect of the stress-inducible promoter RD29A on the expression of the rice FT ortholog Hd3a in Lotus japonicus 'MG20' under environmental changes. Overexpression of Hd3a under the RD29A promoter enabled flowering of 'MG20' under unfavorable weather conditions, while wild type plants could not flower. The inducible expression of florigen has the potential to overcome unfavorable weather conditions and enhance plant production.
Review
Biochemistry & Molecular Biology
Ivan Kreft, Alena Vollmannova, Judita Lidikova, Janette Musilova, Mateja Germ, Aleksandra Golob, Blanka Vombergar, Darja Kocjan Acko, Zlata Luthar
Summary: Tartary buckwheat and common buckwheat are adapted to grow in harsh conditions of high altitudes. The synthesis of UV-B defense metabolites, including flavonoids and rutin, is genetically conditioned and depends on the intensity of ultraviolet radiation. These substances are formed in the leaves and flowers of both buckwheat species, while the concentration of rutin in Tartary buckwheat grain is significantly higher. Flavonoids have protective functions against pests and diseases in plants and also play an important role in human nutrition and the prevention of chronic diseases.
Article
Biochemistry & Molecular Biology
Nadine Werner, Sebastiaan Werten, Jens Hoppen, Gottfried J. Palm, Michael Goettfert, Winfried Hinrichs
Summary: Bradyrhizobium diazoefficiens, a bacterial symbiont of leguminous plants such as soybean, has a regulator protein FrrA that responds to isoflavonoids. The crystal structures of FrrA show a TetR-like fold with a flexible DNA-binding domain, and binding with genistein or naringenin does not induce conformational changes. Small-angle X-ray scattering experiments confirm the flexibility of the DNA-binding domain and lack of structural changes upon ligand binding.
Article
Plant Sciences
Margarita Garcia-Calderon, Marco Betti, Antonio J. Marquez, Jose M. Ortega, Mercedes Roncel
PHYSIOLOGIA PLANTARUM
(2019)
Article
Agronomy
Carmen M. Perez-Delgado, Margarita Garcia-Calderon, Maria Dolores Monje-Rueda, Antonio J. Marquez, Marco Betti
Review
Plant Sciences
Margarita Garcia-Calderon, Carmen M. Perez-Delgado, Peter Palove-Balang, Marco Betti, Antonio J. Marquez
Article
Plant Sciences
Adriana Eliasova, Richard Hrivnak, Petra Stefanova, Marek Svitok, Judita Kochjarova, Helena Otahelova, Milan Novikmec, Peter Pal'ove-Balang
Summary: High ammonium concentration above 10μM limits the presence of Ceratophyllum demersum in water bodies, while nitrate has no adverse effects on it. The plants grow well at 100μM ammonium but are affected by higher concentrations. Laboratory experiments show that ammonium induces the accumulation of flavonoids with antioxidant properties.
Article
Plant Sciences
Maria Kaducova, Adriana Eliasova, Kristina Trush, Michaela Bacovcinova, Katarina Sklenkova, Peter Palove-Balang
Summary: UV-B irradiation induced changes in the concentration of flavonoid compounds in Lotus corniculatus, affecting the expression of key enzymes and leading to accumulation of certain isoflavonoids.
THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Daniel Marino, Rafael A. Canas, Marco Betti
Summary: Agriculture is challenged with adapting to climate change while reducing environmental impact and maintaining or increasing crop yields. The plastidic glutamine synthetase (GS2) enzyme is considered a key candidate for improving crop performance, but its absence in Arabidopsis confers tolerance towards ammonium stress, contradicting previous assumptions. This article opens the debate on whether more or less GS2 is a desirable trait for plant productivity.
Article
Plant Sciences
K. Trush, A. Eliasova, M. D. Monje-Rueda, V. Kolarcik, M. Betti, P. Pal'ove-Balang
Summary: Leaves of Lotus japonicus infected by powdery mildew accumulate isoflavonoids, especially vestitol and sativan as protective compounds. The application of methyl jasmonate showed a moderate effect on the accumulation of vestitol, while chitosan application led to a strong increase in vestitol content. Furthermore, chitosan also induced the expression of key enzymes involved in vestitol and sativan biosynthesis.
BIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Jennifer Mesa-Marin, Enrique Mateos-Naranjo, Joao Carreiras, Eduardo Feijao, Bernardo Duarte, Ana Rita Matos, Marco Betti, Carmen Del Rio, Marina Romero-Bernal, Joan Montaner, Susana Redondo-Gomez
Summary: In this study, the combined effect of increased temperature and atmospheric CO2, salt and drought stress, and PGPR inoculation on the growth and nutritional parameters of Salicornia ramosissima, an edible halophyte, was investigated. It was found that these stressors led to significant changes in fatty acids, phenols, and oxalate contents of S. ramosissima, which are important compounds for human health. Results suggest that in future climate change scenarios, there will be alterations in the lipid profile and levels of oxalate and phenolic compounds in S. ramosissima due to temperature, CO2, and salt and drought stress. The effect of PGPR inoculation depended on the strains used, with some strains inducing the accumulation of phenols and oxalate. This study highlights the potential for nutritional and economic valorization of S. ramosissima in response to these stressors and environmental conditions.
Review
Plant Sciences
Kristina Trush, Peter Pal'ove-Balang
Summary: Isoflavonoids are important phenolic compounds in legumes that offer significant health benefits for humans. They play crucial roles in plant adaptation as defensive compounds and signaling molecules in symbiotic nitrogen fixation and rhizobia. They also accumulate under abiotic stress and are involved in antioxidant defense, UV radiation absorption, and metal chelation. Understanding their functions and cross-reactions with biotic and abiotic stress responses is essential for improving the defense capacity of legume species under changing climatic conditions.
Review
Plant Sciences
Angeles Aroca, Inmaculada Garcia-Diaz, Margarita Garcia-Calderon, Cecilia Gotor, Antonio J. Marquez, Marco Betti
Summary: Photorespiration plays a fundamental role in plant metabolism and its regulation, including transcriptional and post-translational regulation, has been a topic of interest. The interconnections between photorespiration and nitrogen and sulfur metabolism have also been explored. Recent findings on sulfide signaling and protein persulfidation are discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)