Journal
JOURNAL OF PHYSICS D-APPLIED PHYSICS
Volume 52, Issue 20, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6463/ab092f
Keywords
ImageJ; Fiji; image analysis; super-resolution; quantitative imaging; SMLM
Categories
Funding
- Academy of Medical Sciences/the British Heart Foundation/the Government Department of Business, Energy and Industrial Strategy/the Wellcome Trust Springboard Award [SBF003\1163]
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ImageJ is a versatile and powerful tool for quantitative image analysis in microscopy. It is open-source software, platform-independent and enables students and researchers to obtain an easy but thorough introduction into image analysis. Especially the image processing package Fiji is a valuable and powerful extension of ImageJ. Several plugins and macros for single-molecule localization microscopy (SMLM) have been developed during the last decade. These novel tools cover the steps from single-molecule localization and image reconstruction to SMLM data postprocessing such as density analysis, image registration or resolution estimation. This article describes how ImageJ/Fiji can be used for image analysis, reviews existing extensions for SMLM, and aims at introducing and motivating novices and advanced SMLM users alike to explore the possibilities of ImageJ/Fiji for automated and quantitative data analysis.
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