Article
Multidisciplinary Sciences
Phillip A. Frankino, Talha F. Siddiqi, Theodore Bolas, Raz Bar-Ziv, Holly K. Gildea, Hanlin Zhang, Ryo Higuchi-Sanabria, Andrew Dillin
Summary: The transcription factor SKN-1/NRF2 can sense and respond to changes in metabolic state. Knockdown of the amidohydrolase T12A2.1/amdh-1 activates a unique subset of SKN-1 regulated genes, independent of P38-MAPK signaling components. Histidine catabolism genes are required for this activation, possibly through accumulation of a specific catabolite. Activating SKN-1 results in increased oxidative stress resistance but decreased survival to heat stress.
Article
Biology
Samuel J. P. Taylor, Laura Bel Borja, Flavie Soubigou, Jack Houston, Dhanya K. Cheerambathur, Federico Pelisch
Summary: Phosphorylation is an important post-translational modification used for regulating protein localization and activity. Polo-like kinase 1 (PLK-1) is crucial for mitotic and meiotic cell divisions, but its specific roles and regulation in meiosis have been unclear. In this study, using C. elegans oocytes, we demonstrate that PLK-1 is involved in spindle assembly, chromosome alignment and segregation, and polar body extrusion during meiosis I. We also uncover the mechanisms of PLK-1 recruitment to meiotic chromosomes. Our findings reveal the essential functions of PLK-1 in oocyte meiosis and provide insights into its targeting mechanisms.
Article
Chemistry, Analytical
Sian Liao, Xiao-Lei Liu, Katherine E. Manz, Kurt D. Pennell, Joseph Novak, Ewerton Santos, Yongsong Huang
Summary: Alkenones are widely used as paleotemperature biomarkers, and the traditional methods of analysis, such as GC-FID and GC-CI-MS, face challenges with matrix interference and low concentrations. This study demonstrates that HPLC-MS methods provide excellent resolution, selectivity, linearity, and sensitivity for alkenones in complex matrices. The comparison of different mass detectors and ionization modes reveals the advantages of ESI and orbitrap MS for alkenone analyses, with orbitrap MS showing the lowest limit of detection and widest linear dynamic range.
Article
Biochemistry & Molecular Biology
De-Yi Huang, Meng-Jiy Wang, Jih-Jen Wu, Yu-Chie Chen
Summary: ASAI is a new atmospheric surface-assisted ionization method that can ionize both polar and non-polar analytes. By placing samples with high vapor pressure under a titanium slab near the inlet of the mass spectrometer, analytes can be readily ionized and detected.
Article
Biology
Josh Saul, Takashi Hirose, H. Robert Horvitz
Summary: Cell identity is established by gene expression, cell morphology, and cellular function. In this study, the researchers identified two genes, ctbp-1 and egl-13, which are essential for maintaining the identity of the AIA interneurons in Caenorhabditis elegans. They found that ctbp-1 is responsible for maintaining proper AIA gene expression, morphology, and function, while egl-13 regulates AIA function and gene expression. The researchers propose that transcriptional corepressors like CTBP-1 may play a critical role in maintaining cell identities.
Article
Nutrition & Dietetics
Haiyan Chen, Rongji Li, Feng Zhao, Li Luan, Tiantian Han, Zhong Li
Summary: Studies have shown that betulinic acid (BA), a natural product derived from birch bark, has beneficial effects on the lifespan and stress resistance of Caenorhabditis elegans. The optimal dose of BA extended the lifespan, improved the healthspan, and increased oxidative stress resistance in C. elegans. The protective effects of BA on lifespan and antioxidant activity were mediated by the downstream effector DAF-16, which is part of the insulin/IGF-1 receptor signaling pathway.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Lukas Babylon, Fabian Schmitt, Yannik Franke, Tim Hubert, Gunter P. Eckert
Summary: In Alzheimer's disease, folic acid, magnesium, and vitamin B6 can reduce Aβ levels, enhance lactate dehydrogenase expression, and improve the phenotype of nematodes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Khairun Nisaa, Anat Ben-Zvi
Summary: Muscle proteostasis is regulated by the myogenic transcription factor MyoD. This study demonstrates that MyoD can modulate chaperone expression in terminally differentiated muscle cells, indicating its role in muscle maintenance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yizhou Jiang, Uma Gaur, Zhibai Cao, Sheng-Tao Hou, Wenhua Zheng
Summary: This study found that quetiapine shortened the lifespan of C. elegans by acting on dopamine D2 receptors and the mTOR signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Romane Leboutet, Celine Largeau, Leonie Mueller, Magali Prigent, Gregoire Quinet, Manuel S. Rodriguez, Marie-Helene Cuif, Thorsten Hoppe, Emmanuel Culetto, Christophe Lefebvre, Renaud Legouis, Hong Zhang
Summary: The proteins Atg8/LC3/GABARAP are crucial for various steps of autophagy, including initiation, cargo recognition and engulfment, vesicle closure and degradation. In C. elegans, the single homologs of LC3 and GABARAP, LGG-1 and LGG-2, play similar roles. Through mutagenesis experiments, we discovered that LGG-1 is essential for autophagy and development in C. elegans, but its functions can be fully achieved without its localization to the membrane.
Article
Multidisciplinary Sciences
Andrea Annibal, Rebecca George Tharyan, Maribel Fides Schonewolff, Hannah Tam, Christian Latza, Markus Max Karl Auler, Adam Antebi
Summary: The study identifies a shared regulation of the folate and methionine cycles in canonical longevity pathways, with genetic manipulation and supplementation of metabolites showing a causal role in longevity regulation. This comparative approach reveals key metabolic nodes to enhance healthy ageing.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Le Zhang, Yuxing Wang, Dayong Wang
Summary: Paeoniflorin, the major active compound of total glycoside of paeony, can effectively inhibit the immunosuppression induced by bacterial pathogen infection in Caenorhabditis elegans.
ARCHIVES OF PHARMACAL RESEARCH
(2023)
Letter
Medicine, General & Internal
Steven Woloshin, Barnett S. Kramer
Summary: This article discusses the role and limitations of graphs, as well as how to avoid being misled by them. It also mentions a recent journal article that featured a graph considered to be misleading.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Multidisciplinary Sciences
Tatsuya Shioda, Ittetsu Takahashi, Kensuke Ikenaka, Naonobu Fujita, Tomotake Kanki, Toshihiko Oka, Hideki Mochizuki, Adam Antebi, Tamotsu Yoshimori, Shuhei Nakamura
Summary: Accumulating evidence suggests that the molecular network involving neuronal MML-1 function is crucial for regulating systemic aging. This study demonstrates that MML-1 and its heterodimer partners play a primary role in neurons to extend longevity in germlineless animals, acting via downstream cascades that are distinct from HLH-30. The MML-1-GLT-5 axis in neurons is critical for maintaining proteostasis and reducing oxidative stress in long-lived animals through autophagy and peroxidase MLT-7, respectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Mariangela Sabatella, Karen L. Thijssen, Carlota Davo-Martinez, Wim Vermeulen, Hannes Lans
Summary: Research has shown that there are tissue-specific differences in DNA repair activity, with muscle cells appearing to be more resistant to the effects of DNA damage compared to neurons.
Article
Biochemistry & Molecular Biology
Andrea Annibal, Thomas Riemer, Olga Jovanovic, Dennis Westphal, Eva Griesser, Elena E. Pohl, Juergen Schiller, Ralf Hoffmann, Maria Fedorova
FREE RADICAL BIOLOGY AND MEDICINE
(2016)
Article
Biochemical Research Methods
Andrea Annibal, Graziano Colombo, Aldo Milzani, Isabella Dalle-Donne, Maria Fedorova, Ralf Hoffmann
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2016)
Article
Chemistry, Analytical
Andrea Annibal, Oezlem Karalay, Christian Latza, Adam Antebi
ANALYTICAL METHODS
(2018)
Article
Biochemical Research Methods
Rahel Dabrowski, Roberto Ripa, Christian Latza, Andrea Annibal, Adam Antebi
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Cell Biology
Kira Allmeroth, Christine S. Kim, Andrea Annibal, Andromachi Pouikli, Janis Koester, Maxime J. Derisbourg, Carlos Andres Chacon-Martinez, Christian Latza, Adam Antebi, Peter Tessarz, Sara A. Wickstroem, Martin S. Denzel
Summary: The study investigates the role of polyamines in stem cell fate decisions using HFSC organoids, revealing a translation-independent function of polyamines in cell fate regulation. N1-acetylspermidine is identified as a determinant of cell fate, promoting self-renewal and maintaining stemness. This research highlights the diverse pathways of polyamine metabolism-mediated regulation of stem cell fate decisions.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemical Research Methods
Andrea Annibal, Roberto Ripa, Eugen Ballhysa, Christian Latza, Nadine Hochhard, Adam Antebi
Summary: Cyclic dinucleotides (CDNs) are critical secondary messenger molecules that trigger cellular signaling cascades. cGAS plays a key role in synthesizing 2'3' cGAMP, which in turn regulates immune function and antiviral responses. The study presents a novel LC-MS/MS method for quantifying CDNs and highlights the importance of parameter optimization for sensitivity and accuracy.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Correction
Multidisciplinary Sciences
Andrea Annibal, Rebecca George Tharyan, Maribel Fides Schonewolff, Hannah Tam, Christian Latza, Markus Max Karl Auler, Sebastian Groenke, Linda Partridge, Adam Antebi
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Andrea Annibal, Rebecca George Tharyan, Maribel Fides Schonewolff, Hannah Tam, Christian Latza, Markus Max Karl Auler, Adam Antebi
Summary: The study identifies a shared regulation of the folate and methionine cycles in canonical longevity pathways, with genetic manipulation and supplementation of metabolites showing a causal role in longevity regulation. This comparative approach reveals key metabolic nodes to enhance healthy ageing.
NATURE COMMUNICATIONS
(2021)
Meeting Abstract
Rheumatology
J. Steiner, A. Antebi, A. Annibal, T. Kubacki
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Cell Biology
Sonia Ravanelli, Qiaochu Li, Andrea Annibal, Aleksandra Trifunovic, Adam Antebi, Thorsten Hoppe
Summary: Using genetics and multi-omics analyses, researchers have found a link between branched-chain amino acid metabolism and the regulation of the ubiquitin-proteasome system. This sheds light on the role of branched-chain amino acids in the aging process and opens up possibilities for future therapeutic interventions.
Article
Rheumatology
Joachim D. Steiner, Andrea Annibal, Raymond Laboy, Marie Braumann, Heike Goebel, Valentin Laasch, Roman-Ulrich Mueller, Martin R. Spaeth, Adam Antebi, Torsten Kubacki
Summary: In this study, a family with a novel mutation in the TNFRSF1A gene causing TRAPS and renal AA amyloidosis was described. Plasma metabolome analysis revealed dysregulated metabolic pathways and identified potential biomarkers. Canakinumab showed effectiveness in treating patients with this mutation. The findings contribute to understanding the pathophysiology of TRAPS and have clinical implications for treatment.
Article
Multidisciplinary Sciences
Eunah Kim, Andrea Annibal, Yujin Lee, Hae-Eun H. Park, Seokjin Ham, Dae-Eun Jeong, Younghun Kim, Sangsoon Park, Sujeong Kwon, Yoonji Jung, JiSoo Park, Sieun S. Kim, Adam Antebi, Seung-Jae V. Lee
Summary: Mitochondrial Krebs cycle plays a crucial role in immunity, and the genetic inhibition of mitochondrial aconitase can enhance immunity against pathogenic bacteria.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Richard A. Coch, Florian Geisler, Andrea Annibal, Adam Antebi, Rudolf E. Leube
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Endocrinology & Metabolism
Rebecca George Tharyan, Andrea Annibal, Isabelle Schiffer, Raymond Laboy, Ilian Atanassov, Anna Luise Weber, Birgit Gerisch, Adam Antebi