Journal
JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
Volume 56, Issue 3, Pages 277-285Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10601325.2019.1565549
Keywords
Polymeric microbeads; immobilization; laccase; pollutants; synthetic dyes
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Funding
- Scientific and Technological Research Council of Turkey [113Z427]
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Laccase from Trametes versicolar was immobilized on to acrylate based microbeads carrying epoxy or carbonate groups. The amounts of immobilized enzyme on the carbonate and epoxy groups were 24.6 and 9.7 mg/g, respectively. In the presence of a mediator (i.e., acetosyringone) almost 100% biodegradation was observed for both Methylene Blue dye (MB) and Carbaryl pesticide (CP) by the immobilized laccases, while in the absence of mediator, 63 and 71%, respectively. The immobilized enzyme was operated in a fluidized bed reactor for biodegradation of MB and CP for 24 h, and the initial activity lost was only 8.0 and 2.0%, respectively. The data show that the immobilized enzyme can effectively be utilized for complete degradation of organic pollutants in wastewaters.
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