4.3 Article

Genetic diversity of Iranian and some European grapes as revealed by nuclear and chloroplast microsatellite and SNP molecular markers

Journal

JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
Volume 94, Issue 5, Pages 599-610

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/14620316.2019.1585210

Keywords

Genetic diversity; microsatellite; single nucleotide polymorphism; synonymy; Vitis vinifera

Categories

Funding

  1. University of Kurdistan [4.1300]

Ask authors/readers for more resources

Genetic and chloroplast haplotype variations of 35 Iranian genotypes and 10 European grape cultivars were investigated using 9 nuclear simple sequence repeats (nSSRs), 4 chloroplast simple sequence repeats (cpSSRs) and 46 single nucleotide polymorphism (SNP) markers. In total, 83 alleles were detected at nine nSSRs, giving a mean of 15.66 alleles per locus and polymorphism information content (PIC) values >= 0.75 ranged from 0.75 to 0.90. For SNP markers, PIC values varied from 0.30 to 0.39 with an average of 0.34. Analysis of molecular variance revealed 97 and 93% of partitioned genetic diversity within populations using nSSRs and SNPs markers, respectively. Un-weighted neighbour-joining (NJ) cluster analysis grouped grapes into 10 and 9 major clusters using SSR and SNP markers, respectively. Synonyms and homonyms were identified among the Iranian genotypes. Close genetic relationship among Farkhi and Bidane-Sefid genotypes may probably propose a common ancestor and mutational evolution. Most European cultivars were differentiated from Iranian genotypes, however, clustering of some Iranian genotypes with European cultivars in the same clusters suggests that clonally propagated materials have probably been exchanged from the Middle East to West or vice versa. C and D chloroplast haplotypes were the most frequent within the Iranian genotypes, while A chloroplast haplotype was exclusively observed among European cultivars.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Agronomy

Potassium Silicate Improves Salinity Resistant and Affects Fruit Quality in Two Strawberry Cultivars Grown Under Salt Stress

Khatere Yaghubi, Yavar Vafaee, Naser Ghaderi, Taimoor Javadi

COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS (2019)

Article Environmental Sciences

Selenium mitigates cadmium toxicity by preventing oxidative stress and enhancing photosynthesis and micronutrient availability on radish (Raphanus sativus L.) cv. Cherry Belle

Setareh Auobi Amirabad, Farhad Behtash, Yavar Vafaee

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)

Article Plant Sciences

Molecular characterization of endangered Iranian terrestrial orchids using ISSR markers and association with floral and tuber-related phenotypic traits

Soheila Gholami, Yavar Vafaee, Farzad Nazari, Abdolbaset Ghorbani

Summary: The study investigated the genetic diversity, population structure, and phylogeny of Iranian orchids using ISSR markers to discover markers associated with phenotypic traits. Results showed high correlations between floral and tuber-related phenotypic traits, which were associated with some ISSR bands.

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (2021)

Article Plant Sciences

Phenotypic characterization and seed-micromorphology diversity of the threatened terrestrial orchids: implications for conservation

Yavar Vafaee, Ghane Mohammadi, Farzad Nazari, Mozhgan Fatahi, Aboubakr Kaki, Soheila Gholami, Abdolbaset Ghorbani, Ali Khadivi

Summary: The study evaluated phenotypic and seed-micromorphology variability of 106 accessions from different orchid species. Most traits showed low coefficient variations, mainly focused on economically valuable traits. The highest mean values for tuber traits were observed in a specific population of Himantoglossum affine.

SOUTH AFRICAN JOURNAL OF BOTANY (2021)

Article Plant Sciences

Exploring genetic variations in threatened medicinal orchids using start codon targeted (SCoT) polymorphism and marker-association with seed morphometric traits

Soheila Gholami, Yavar Vafaee, Farzad Nazari, Abdolbaset Ghorbani

Summary: The study investigated the genetic diversity and population structure of eight Iranian terrestrial orchid species using SCoT markers and found associations between certain markers and seed morphometric traits. Results showed variations in allele numbers and polymorphism levels among different regions and species of orchids. Multiple association analysis identified significant correlations between specific markers and seed morphometric traits.

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (2021)

Article Biotechnology & Applied Microbiology

In vitro mutagenesis of Chrysanthemum morifolium cultivars using ethylmethanesulphonate (EMS) and mutation assessment by ISSR and IRAP markers

Fardin Nasri, Hedayat Zakizadeh, Yavar Vafaee, Ali Akbar Mozafari

Summary: The study focused on mutagenesis of chrysanthemum cultivars using EMS as mutagen, resulting in a total of 2082 plantlets and 58 mutants with phenotypic and molecular variations. ISSR and IRAP markers successfully classified the mutants and their relationship with mother plants, demonstrating the effectiveness of in vitro EMS-induced mutation for breeding new chrysanthemum cultivars.

PLANT CELL TISSUE AND ORGAN CULTURE (2022)

Article Plant Sciences

Gamma-aminobutyric acid (GABA) and salinity impacts antioxidative response and expression of stress-related genes in strawberry cv. Aromas

Sahar Golnari, Yavar Vafaee, Farzad Nazari, Naser Ghaderi

Summary: Under salinity stress conditions, GABA can reduce H2O2 and MDA content, maintain MSI percentage, enhance antioxidant enzyme activity, and increase the transcription level of salinity-related genes.

BRAZILIAN JOURNAL OF BOTANY (2021)

Article Plant Sciences

Impact of Rice Husk Biochar on Drought Stress Tolerance in Perennial Ryegrass (Lolium perenne L.)

Shabnam Safari, Farzad Nazari, Yavar Vafaee, Jaime A. Teixeira da Silva

Summary: The use of rice husk biochar (RHB) can improve soil properties and nutrient uptake, enhancing the growth and drought tolerance of perennial ryegrass.

JOURNAL OF PLANT GROWTH REGULATION (2023)

Article Chemistry, Physical

Silicon Nanoparticles and Methyl Jasmonate Improve Physiological Response and Increase Expression of Stress-related Genes in Strawberry cv. Paros Under Salinity Stress

Parisa Moradi, Yavar Vafaee, Ali Akbar Mozafari, Nawroz Abdul-razzak Tahir

Summary: This study examines the effects of methyl jasmonate (MeJA) and silicon nanoparticles on strawberry plants under salt stress. The results show that MeJA and silicon nanoparticles can reduce oxidative stress, increase antioxidant content, and enhance the transcription level of salt-related genes.

SILICON (2022)

Article Plant Sciences

In vitro asymbiotic seed germination, protocorm formation, and plantlet development of Orchis simia Lam.: A threatened terrestrial orchid species

Mozhgan Fatahi, Yavar Vafaee, Farzad Nazari, Nawroz Abdul-razzak Tahir

Summary: The present study investigated the seed morphometric, viability, asymbiotic seed germination, protocorm formation, and plantlet growth of the endangered terrestrial orchid species Orchis simia Lam. It was found that the addition of pineapple juice and casein hydrolysate to the media significantly improved seed germination percentage. Furthermore, treatments with higher germination percentage, faster germination, and bigger protocorms resulted in better plantlet growth. This study is important for the large-scale multiplication of in vitro raised seedlings to reintroduce them into endangered regions.

SOUTH AFRICAN JOURNAL OF BOTANY (2022)

Article Biodiversity Conservation

Large-Scale In Vitro Multiplication and Phytochemical Analysis of Himantoglossum affine (Boiss.) Schltr.: An Endangered Euro-Mediterranean Terrestrial Orchid

Mozhgan Fatahi, Yavar Vafaee, Nawroz Abdul-Razzak Tahir, Jalal Khorshidi

Summary: Himantoglossum affine is a threatened terrestrial orchid. In this study, we optimized asymbiotic seed germination and direct embryogenesis, and analyzed the phytochemical profile and physico-biochemical analysis of leaf and tuber. The use of organic nitrogen compounds resulted in higher germination efficiencies, and coconut water plus casein hydrolysate promoted the shortest times to germination. Plantlets grown on media supplemented with pineapple juice and peptone showed the highest plantlet length and weight. For embryogenesis, the highest regeneration rate and embryo number were observed in young protocorm-like body (PLB) explants with specific concentrations of naphthalene acetic acid (NAA) and thidiazuron (TDZ). Gas chromatography-mass spectrometry analysis identified several compounds as the most prevalent in the plant. Furthermore, the study reported higher contents of total phenolics and flavonoids, as well as antioxidant activity compared to other terrestrial orchids. Based on the fragmentation of H. affine populations in certain regions, the study's findings can be utilized for the re-introduction of in vitro-raised plants in threatened areas.

DIVERSITY-BASEL (2022)

Article Plant Sciences

Storability and postharvest quality of five Iranian grape cultivars during cold storage

Shadieh Ahmadi Soleimanie, Yavar Vafaee

Summary: The research aimed to investigate the postharvest response of five Iranian grape cultivars under cold storage conditions. Total soluble solids (TSS) content increased slowly during cold storage, and berry firmness increased due to water loss. Conversely, the content of total flavonoids (TF), total phenolics (TP), vitamin C, total anthocyanins (TAC), and total antioxidant activity (TAA) decreased during cold storage. Different grape cultivars exhibited significant differences in physicochemical attributes. The results suggest that cold storage is a suitable method to maintain postharvest quality of Iranian grape cultivars and could be valuable for improving their postharvest and nutritional quality in the future.

PLANT PHYSIOLOGY REPORTS (2023)

Article Agronomy

Comparative Analysis of Phospholipase D (PLD) Gene Family in Camelina sativa and Brassica napus and Its Responses in Camelina Seedlings under Salt Stress

Parviz Heidari, Fatemeh Puresmaeli, Yavar Vafaee, Mostafa Ahmadizadeh, Mohammadreza Ensani, Haniyeh Ahmadinia

Summary: This study characterized and compared the phospholipase D (PLD) gene family in two important oilseed crops, Brassica napus and Camelina sativa. The results showed different evolution processes and regulatory element distributions between the two crops. Expression analysis revealed the involvement of CsPLD genes in salt stress response and their enhanced expression with longer stress periods.

AGRONOMY-BASEL (2023)

No Data Available