Mildly acidic conditions eliminate deamidation artifact during proteolysis: digestion with endoprotease Glu-C at pH 4.5

Discovery and Characterization of a Photo-Oxidative Histidine-Histidine Cross-Link in IgG1 Antibody Utilizing 18O-Labeling and Mass Spectrometry

(2014) Min Liu et al.   ANALYTICAL CHEMISTRY

Discovery of a Chemical Modification by Citric Acid in a Recombinant Monoclonal Antibody

(2014) Chris Chumsae et al.   ANALYTICAL CHEMISTRY

Non-repair Pathways for Minimizing Protein Isoaspartyl Damage in the YeastSaccharomyces cerevisiae

(2014) Alexander N. Patananan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Detection and Quantitation of Succinimide in Intact Protein via Hydrazine Trapping and Chemical Derivatization

(2014) Joshua J. Klaene et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation

(2014) Piliang Hao et al.   JOURNAL OF PROTEOME RESEARCH

The use of native gels for the concomitant determination of protein sequences and modifications by mass spectrometry with subsequent conformational and functional analysis of native proteins following electro-elution

(2013) Wei-Qiang Chen et al.   AMINO ACIDS

Integrated Proteomic Analysis of Major Isoaspartyl-Containing Proteins in the Urine of Wild Type and Protein l-Isoaspartate O-Methyltransferase-Deficient Mice

(2013) Shujia Dai et al.   ANALYTICAL CHEMISTRY

Characterization of a Low-Level Unknown Isomeric Degradation Product Using an Integrated Online–Offline Top-Down Tandem Mass Spectrometry Platform

(2013) Xiang Yu et al.   ANALYTICAL CHEMISTRY

Discovery of Undefined Protein Cross-Linking Chemistry: A Comprehensive Methodology Utilizing 18O-Labeling and Mass Spectrometry

(2013) Min Liu et al.   ANALYTICAL CHEMISTRY

Arginine Modifications by Methylglyoxal: Discovery in a Recombinant Monoclonal Antibody and Contribution to Acidic Species

(2013) Chris Chumsae et al.   ANALYTICAL CHEMISTRY

Paraoxonase 1 and homocysteine metabolism

(2012) Joanna Perła-Kaján et al.   AMINO ACIDS

Quantitation of asparagine deamidation by isotope labeling and liquid chromatography coupled with mass spectrometry analysis

(2012) Hongcheng Liu et al.   ANALYTICAL BIOCHEMISTRY

Determination of Deamidation Artifacts Introduced by Sample Preparation Using 18O-Labeling and Tandem Mass Spectrometry Analysis

(2012) Yi Du et al.   ANALYTICAL CHEMISTRY

Substrate-Induced Control of Product Formation by Protein Arginine Methyltransferase 1

(2012) Shanying Gui et al.   BIOCHEMISTRY

Chemical Deamidation: A Common Pitfall in Large-Scale N-Linked Glycoproteomic Mass Spectrometry-Based Analyses

(2012) Giuseppe Palmisano et al.   JOURNAL OF PROTEOME RESEARCH

Protein L-isoaspartyl methyltransferase regulates p53 activity

(2012) Jae-Cheol Lee et al.   Nature Communications

Protein Isoaspartate Methyltransferase-Mediated 18O-Labeling of Isoaspartic Acid for Mass Spectrometry Analysis

(2011) Min Liu et al.   ANALYTICAL CHEMISTRY

Analysis of Isoaspartic Acid by Selective Proteolysis with Asp-N and Electron Transfer Dissociation Mass Spectrometry

(2010) Wenqin Ni et al.   ANALYTICAL CHEMISTRY

Characterization of the Glycosylation Occupancy and the Active Site in the Follow-on Protein Therapeutic: TNK-Tissue Plasminogen Activator

(2010) Haitao Jiang et al.   ANALYTICAL CHEMISTRY

Structural changes induced by the deamidation and isomerization of asparagine revealed by the crystal structure of Ustilago sphaerogena ribonuclease U2B

(2010) Shuji Noguchi   BIOPOLYMERS

Substrates of theArabidopsis thalianaProtein Isoaspartyl Methyltransferase 1 Identified Using Phage Display and Biopanning

(2010) Tingsu Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Human serum albumin modifications associated with reductive radical stress

(2010) Anna Maria Salzano et al.   Molecular BioSystems

Stability of Protein Pharmaceuticals: An Update

(2010) Mark Cornell Manning et al.   PHARMACEUTICAL RESEARCH

Desulfurization of cysteine-containing peptides resulting from sample preparation for protein characterization by mass spectrometry

(2010) Zhouxi Wang et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Chemical Methods for the Detection of Protein N-Homocysteinylation via Selective Reactions with Aldehydes

(2009) Tianzhu Zang et al.   ANALYTICAL CHEMISTRY

Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds

(2009) Petr Pompach et al.   JOURNAL OF MASS SPECTROMETRY

Separation of peptide isomers and conformers by ultra performance liquid chromatography

(2009) Dominic Winter et al.   JOURNAL OF SEPARATION SCIENCE

Chemo-Enzymatic Detection of Protein Isoaspartate Using Protein Isoaspartate Methyltransferase and Hydrazine Trapping

(2008) Joshua F. Alfaro et al.   ANALYTICAL CHEMISTRY

Use of 18O Labels to Monitor Deamidation during Protein and Peptide Sample Processing

(2008) Xiaojuan Li et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY