Journal
JOURNAL OF FOOD BIOCHEMISTRY
Volume 43, Issue 4, Pages -Publisher
WILEY
DOI: 10.1111/jfbc.12794
Keywords
adventitious roots; cold stimulation; genes; HPLC-MSn; temperature
Funding
- National Natural Science Foundation of China NSFC [81703639]
- Central Significant Increase or Decrease Program of China [2060302]
Ask authors/readers for more resources
This study researched the effect of temperature on growth and ginsenosides accumulation in adventitious root cultures of Panax ginseng. Results showed that the ginseng adventitious roots growth and differentiation ability could be affected faced with different incubation temperatures (15, 20, 25, and 30 degrees C for 35 days). Besides, the research also demonstrated that low-temperature stimulation could promote the accumulation of ginsenosides and the content of total ginsenosides increased by 2.53 times at 10 degrees C-7d (10 degrees C for 7 days and then transferred to 25 degrees C for 28 days) compared with that at 25 degrees C. Moreover, the transcriptional levels of functional genes and PgWRKYs were analyzed by this study and the correlation analysis showed that GPS, SS, CYP716A47, CYP716A53v2, UGT74AE2, UGT94Q2, PgWRKY1, PgWRKY3, and PgWRKY8 were significantly correlated with total ginsenosides content. Furthermore, HPLC-ESI-MSn analyzed that Malonyl-Rb-1 only existed in 10 degrees C-7d group. Practical applications The survey showed that after a certain time of stimulating P. ginseng adventitious roots at low temperature, the accumulation of ginsenosides could be enhanced as their expression of related genes were regulated. It provides a theoretical foundation for the mass production of ginsenosides by controlling the temperature conditions of P. ginseng adventitious roots.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available