Journal
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
Volume 94, Issue 1, Pages 143-146Publisher
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.15-0571
Keywords
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Funding
- National Institute for Allergy and Infectious Diseases (NIAID) [R01AI089819, R21AI101427, R21 AI103466, R01AI107949]
- National Institute of General Medicine Sciences [T32GM008719, T32GM007092, F30AI109979]
- IDSA Medical Scholars Program
- U.S. Department of Defense Global Emerging Surveillance Program
- Swedish Development Cooperation Agency (SIDA) [Bil-Tz 16/9875007059]
- Swedish Medical Research Council [2013-6594]
- Institut Pasteur de Madagascar
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Polymorphisms within Plasmodium falciparum vaccine candidate antigens have the potential to compromise vaccine efficacy. Understanding the allele frequencies of polymorphisms in critical binding regions of antigens can help in the designing of strain-transcendent vaccines. Here, we adopt a pooled deep-sequencing approach, originally designed to study P. falciparum drug resistance mutations, to study the diversity of two leading transmission-blocking vaccine candidates, Pfs25 and Pfs48/45. We sequenced 329 P. falciparum field isolates from six different geographic regions. Pfs25 showed little diversity, with only one known polymorphism identified in the region associated with binding of transmission-blocking antibodies among our isolates. However, we identified four new mutations among eight non-synonymous mutations within the presumed antibody-binding region of Pfs48145. Pooled deep sequencing provides a scalable and cost-effective approach for the targeted study of allele frequencies of P falciparum candidate vaccine antigens.
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