Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 107, Issue 5, Pages 1001-1013Publisher
WILEY
DOI: 10.1002/jbm.a.36626
Keywords
octacalcium phosphate; hydroxyapatite; beta-tricalcium phosphate; osteoclast-osteoblast crosstalk
Funding
- Ministry of Education, Science, Sports and Culture of Japan (MEXT) [23106010, 17K19740, 18H02981, 16K20482]
- Tohoku University Center for Gender Equality Promotion (TUMUG) Support Project (Project to Promote Gender Equality and Female Researchers)
- Grants-in-Aid for Scientific Research [16K20482, 18H02981, 17K19740] Funding Source: KAKEN
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Previous studies have reported that octacalcium phosphate (OCP) enhances osteoblast differentiation and osteoclast formation during the hydrolysis process to hydroxyapatite (HA). However, the crystal phases that affect the crosstalk between osteoclasts and osteoblasts are unknown, which should determine the bone substitute material's property of OCP. The present study was designed to investigate whether the chemical composition and crystal structure of calcium phosphates affect osteoclast formation and the osteoclast-osteoblast crosstalk. Biodegradable beta-tricalcium phosphate (beta-TCP) was used as the control material. Osteoclasts were cultured on HA/OCP or HA/TCP disks and their cellular responses were assessed. Both OCP and beta-TCP had a similar ability to create multinucleated osteoclasts. However, OCP promoted the expression of complement component 3a (C3a), a positive coupling factor, in osteoclasts, whereas beta-TCP enhanced that of EphrinB2 (EfnB2) and collagen triple helix repeat containing 1 (Cthrc1). During osteoclast culture, phosphate ions were released from the crystals, and OCP-HA conversion was advanced in HA/OCP mixtures and OCP. X-ray diffraction analysis revealed no remarkable changes in the crystal structures of HA/TCP mixtures and beta-TCP before and after osteoclast culture. These results indicate that the distinct chemical environment induced by the calcium phosphate phases affects the crosstalk between osteoclasts and osteoblasts. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1001-1013, 2019.
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