Article
Agronomy
Zsolt Csenki, Eduard Gubo, Edina Garai, Katalin Bakos, Dora Kanaine Sipos, Erna Vasarhelyi, Bela Urbanyi, Pal Szakal, Judit Plutzer
Summary: The study conducted tests on estrogen content in slurry-based irrigation water from dairy cattle farms using a combination of the newly developed in vivo microinjection method and classical in vitro methods. Results showed that this combined approach proved to be a highly effective screening tool for samples containing high concentrations of organic matter.
AGRICULTURAL WATER MANAGEMENT
(2022)
Article
Agronomy
Zsolt Csenki, Eduard Gubo, Edina Garai, Katalin Bakos, Dora Kanaine Sipos, Erna Vasarhelyi, Bela Urbanyi, Pal Szakal, Judit Plutzer
Summary: Livestock farm waste, including manure, is a significant source of estrogenic pollutants in the environment. In this study, a novel in vivo microinjection method was proposed to investigate the estrogen content of slurry-based irrigation water extracts. The combination of in vivo and in vitro methods provides a highly effective screening tool for samples containing high concentrations of organic matter, allowing for a more comprehensive assessment of estrogenicity and toxicity.
AGRICULTURAL WATER MANAGEMENT
(2022)
Article
Biochemistry & Molecular Biology
Flavia Bieczynski, Kathleen Burkhardt-Medicke, Carlos M. Luquet, Stefan Scholz, Till Luckenbach
Summary: An automated microscopy-based rhodamine B dye accumulation assay was established to evaluate the activity of multixenobiotic efflux transporter in zebrafish embryos, confirming the function of Abcb4 as an efflux transporter in zebrafish and showing potential for screening environmental pollutants and chemicals.
Article
Developmental Biology
Gerardo Mendoza Jr, Adedeji A. Afolalu, Kvin Lertpiriyapong, Neil S. S. Lipman, Christine Lieggi
Summary: The impact of consecutive use of iodine and sodium hypochlorite disinfectants on zebrafish embryo survival and development was evaluated. It was found that using NaOCl disinfectant in media with high conductivity decreased survival rate, but consecutive use of iodine and NaOCl ensured a certain level of survival rate.
Article
Environmental Sciences
Mohamed Ashfaque, A. Nafeez Ahmed, S. Mohammed Safiullah, G. Taju, S. Abdul Majeed, A. S. Sahul Hameed, K. Anver Basha
Summary: Carbon nanotubes (CNTs) have been widely used in developing polymer hybrid coatings for anticorrosive application. In this study, a novel poly [(3,5-dimethyl-lH-pyrazole-1-yl) methyl methacrylate-co-glycidyl methacrylate] (PyM) was prepared and single-wall carbon nanotubes (SWCNT) were incorporated in PyM to form nanocomposites. The toxicity of the nanocomposites was evaluated in vivo and in vitro using zebrafish embryos and gill cell line of zebrafish (DrG). The results showed that nanocomposites with a concentration of 400 μg/ml of PyM in 1.0% of SWCNT were non-toxic and recommended for anticorrosive application. Nanocomposites with higher SWCNT content showed toxic effects. The nanocomposites with 1.5% of SWCNT delayed hatching rate, decreased survival rate and heart beat in zebrafish embryos, and inducedmorphological changes in DrG cells. Gene expression studies revealed that nanocomposites with high SWCNT concentration induced oxidative stress. The immersion study of the recommended concentration of PyM-SWCNT on mild steel (MS) in sea water showed effective corrosion protection without leaching behavior. The findings suggest that the nanocomposites with the novel polymer have potential for industrial anticorrosive applications.
Article
Environmental Sciences
Shaojuan Guo, Bolun Kang, Ruihong Wang, Lixin Yang
Summary: Methylmercury is a neurotoxin that affects visual abilities and causes damage to retinal cells. Exposure to methylmercury in zebrafish embryos resulted in abnormal expression of complement component genes and increased apoptosis in the retina. These effects were specific to methylmercury exposure, as compared to cadmium and arsenic.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Plant Sciences
Hatice Alkan, Ibrahim Hakki Cigerci, Muhammad Muddassir Ali, Omer Hazman, Recep Liman, Florica Cola, Elena Bonciu
Summary: This study evaluated the cytotoxic and genotoxic effects of biosynthesized silver nanoparticles on breast cancer and umbilical vein endothelial cells. The results showed that the synthesized silver nanoparticles exhibited cytotoxic and genotoxic effects on breast cancer cells, but had no significant effects on normal cells.
Article
Anatomy & Morphology
Zachary W. Nurcombe, Carrie Lynn Hehr, Sarah McFarlane
Summary: Growth factors are important for neuron survival in the developing nervous system. Plexina4 promotes cell survival in the embryonic hindbrain of zebrafish.
DEVELOPMENTAL DYNAMICS
(2023)
Article
Environmental Sciences
Rehab Elfadul, Roman Jesien, Ahmed Elnabawi, Paulinus Chigbu, Ali Ishaque
Summary: The study found the presence of estrogenic substances in Maryland Coastal Bays, affecting cell proliferation primarily through estrogen receptor mediation.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Biochemistry & Molecular Biology
Mingxing Wang, Juan Liu, Huiyun Wang, Tingzhang Hu
Summary: This study investigated the effects of Spiromesifen (SPF) on blood vessel development and its mechanism of action. It was found that SPF exposure caused abnormal blood vessel development, inhibited cell migration and proliferation, and disrupted the cytoskeleton via the Rho/ROCK pathway. These findings contribute to a better understanding of the toxic effects of SPF on angiocardiopathy.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Maria J. Carreira, Nicolas Vila-Blanco, Pablo Cabezas-Sainz, Laura Sanchez
Summary: Zebrafish is an ideal model organism for studying human cancer due to its transparency and the development of a pigment-deficient line for direct observation of cancer formation. ZFTool was developed to automatically quantify cancer cell evolution and dissemination, providing a reliable method for personalized medicine development.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Mohammad Amin Jadidi Kouhbanani, Yasin Sadeghipour, Mina Sarani, Erfan Sefidgar, Saba Ilkhani, Ali Mohammad Amani, Nasrin Beheshtkhoo
Summary: In this study, Nickel oxide nanoparticles were synthesized using the sol-gel method and their characteristics were analyzed through various techniques. The results showed that the particle size had an impact on the structural alterations, electrochemical behaviors, and cytotoxic effect of the synthesized NiO NPs. The cytotoxicity of the NiO NPs was higher at 300°C compared to 400°C and 500°C, making them a potentially effective option for cancer treatment against various cancer cell lines.
GREEN CHEMISTRY LETTERS AND REVIEWS
(2021)
Article
Materials Science, Biomaterials
Munirah Jamil, Iskandar Shahrim Mustafa, Naser Mahmoud Ahmed, Shahrul Bariyah Sahul Hamid
Summary: This study aims to evaluate the cytotoxicity of poly(ethylene) oxide (PEO) nanofibers with different concentrations in human breast cancer cell lines (MCF-7) and determine the optimal PEO nanofiber concentration for diagnostic applications. The results showed that the viscosity of the polymer solution played a vital role in producing beadless nanofiber mats. Increasing the PEO concentration resulted in larger nanofiber diameter and thickness, but reduced surface roughness. All PEO nanofiber formulations were non-toxic to MCF-7 cells, with the 5 wt% PEO nanofiber showing the best characteristics for diagnostic purposes.
BIOMATERIALS ADVANCES
(2022)
Article
Chemistry, Applied
Jaggaiah N. Gorantla, Sunaree Choknud, Eko Suyanto, Htun-Htun Win, Yanling Hua, Maniganda Santhi, Sirilak Wangngae, Anyanee Kamkaew, Mariena Ketudat-Cairns, Lalida Rojanathammanee, James R. Ketudat Cairns
Summary: In this study, various hydroxycinnamic and hydroxybenzoic acids were easily converted to phenolic amides (PAMs) through a one-step BOP-mediated amide coupling reaction. Fifteen PAMs were synthesized with yields above 40% and evaluated for cytotoxic activities against four cancer cell lines. Four amides exhibited promising IC50 values ranging from 5 to 55 μM, while tetradecyl-gallic-amide (13) specifically showed significant inhibitory effects on THP-1 leukemia cells with an IC50 value of 3.08 μM. These findings highlight the potential of phenolic amides as anticancer agents.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Jiwon Choi, Gowoon Lee, Sunmi Kim, Kyungho Choi
Summary: The study suggests that DBDPE and BTBPE may disrupt sex hormone regulation in male zebrafish and H295R cells, but likely not through alteration of the steroidogenesis pathway.
APPLIED SCIENCES-BASEL
(2021)