Bioinspired polydopamine coating-assisted electrospun polyurethane-graphene oxide nanofibers for bone tissue engineering application

It is inevitable to replace the tissues and organs that have been malfunctioning due to trauma or difference diseases, so tissue engineering can be a promising approach in case of regeneration. In this investigation, electrospun polyurethane-graphene oxide scaffold was coated with polydopamine (PDA) by immersing the constructs in dopamine hydrochloride solution under the alkaline condition for 24 h. The present study aimed to investigate the synergic effects of graphene oxide (GO) and PDA on the osteogenic expression. Field emission scanning electron microscopy, Fourier transform infrared spectrum, and X-ray diffraction (XRD) proved the synthesis of GO. Water drop contact angle and swelling absorption test indicated significantly enhancement in hydrophilicity after coating the scaffolds with PDA. Besides, PDA induced more ability of mineralization bone-like component. According to the results, cell attachment and proliferation were greatly increased in coated constructs. Alkaline phosphatase expression was also increased on the PDA-deposited polymeric scaffolds. In general, these characteristics suggest that the PDA-coated polyurethane-GO scaffolds are an appropriate substrate for in vivo studies and the bone regeneration. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47656.