Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 67, Issue 8, Pages 2265-2271Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b06427
Keywords
Huanglongbing disease; seasonal variation; navel orange; mass spectrometry
Funding
- Fundamental Research Funds for the Central Universities [21618341]
- National Natural Science Foundation of China [21804053]
- Foundation for New Faculty Start-up Grant of Jinan University
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Citrus greening disease [Huanglongbing (HLB)] is the most destructive disease of citrus. In this work, we have established a metabolite-based mass spectrometry (MS) method for rapid detection of HLB in navel orange trees. Without sample pretreatment, characteristic mass spectra can be directly obtained from the raw plant samples using the direct MS method. The whole detection process can be accomplished within 1 min. By monitoring and comparisons of the healthy and infected plants throughout a whole year, characteristic MS peaks of metabolites are found to be specific responses from infected plants and, thus, could be used as biomarkers for detection of HLB. Therefore, HLB could be directly detected in the asymptomatic samples, such as stems, using this metabolite-based direct MS method. In addition, principal component analysis and partial least squares discriminant analysis modes of metabolites from healthy and infected trees were established for investigating differentiation and seasonal variations of HLB in leaves, veins, and stems, providing valuable information for understanding the HLB in different seasons.
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