Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 559, Issue -, Pages 410-419Publisher
ELSEVIER
DOI: 10.1016/j.ijpharm.2019.01.060
Keywords
Polygonatum sibiricum polysaccharides; Cubosomes; Optimization; Modification; Lymphocyte proliferation
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Funding
- National Natural Science Foundation of China [31672596, 31872509]
- Fundamental Research Funds for the Central Universities [KYZ201844]
- Special Fund for Agro-scientific Research in the Public Interest [201403051]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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This study aimed to optimize the preparation conditions for cetyltrimethylammonium bromide-modified Polygonatum sibiricum polysaccharide cubosomes (CTAB-modified PSP-Cubs) by response surface methodology (RSM). Glyceryl-monooleate (GMO) was used as the lipid base for the cubosomes. The optimal preparation conditions of CTAB-modified PSP-Cubs were as follows: the mass percentage of PSP to GMO (X-1), Poloxamer 407 (F127) to GMO (X-2) and water to GMO (X-3) was 1.4%, 9% and 50%, respectively. The encapsulation efficiency (EE) of CTAB-modified PSP-Cubs under the experimental conditions was 62.4 +/- 3.6%, which was close to our predicted value. The particle size, polydispersity index (PDI) and zeta potential of CTAB-modified PSP-Cubs were 427.7 +/- 8.0 nm, 0.236 +/- 0.024 and 19.2 +/- 0.4 mV, respectively. Specific modifications were able to slow down in vitro release behaviors and reduce cytotoxicity to some extent. The effects of CTAB-modified PSP-Cubs on splenic lymphocytes were also investigated. When splenic lymphocytes were treated with CTAB-modified PSP-Cubs together with LPS or PHA, the results showed more favorable effects on cellular proliferation than blank cubosomes or free PSP at certain concentrations.
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