Article
Biochemistry & Molecular Biology
Christopher Simon, Tomoko Soga, Nafees Ahemad, Saatheeyavaane Bhuvanendran, Ishwar Parhar
Summary: This study discovers a neuropeptide, KP-10, that can alleviate the damage caused by amyloid-beta and alpha-synuclein in cholinergic neurons. KP-10 mitigates alpha-synuclein-induced toxicity in cholinergic neurons through preferential binding. Moreover, in vitro and in silico experiments support this observation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Nikita Mundhara, Shital Yadav, Pallavi Uday Shirke, Dulal Panda, Abhijit Majumder
Summary: This study investigates the impact of substrate loss modulus on neuronal differentiation, highlighting that high loss modulus can promote neuronal differentiation independently of storage modulus. The combined effects of loss modulus and retinoic acid-induced chemical stimulation were also studied, with the highest neuronal differentiation observed on high loss modulus substrate. This research emphasizes the importance of integrating mechanical and chemical cues in cellular differentiation processes.
Article
Environmental Sciences
Churaibhon Wisessaowapak, Daranee Visitnonthachai, Piyajit Watcharasit, Jutamaad Satayavivad
Summary: The study demonstrated that prolonged exposure to low doses of sodium arsenite may increase tau phosphorylation in neurons, partly through activation of GSK3 and ERK1/2 signaling pathways.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Giuseppe Uras, Xinuo Li, Alessia Manca, Antonella Pantaleo, Marco Bo, Jinyi Xu, Stephanie Allen, Zheying Zhu
Summary: In this study, a novel cell model was developed to evaluate potential drugs for treating Alzheimer's disease. Testing on the model revealed that inhibiting acetylcholinesterase can improve disease-induced defects, and dual inhibition further enhances the effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Qisheng Tang, Tianwen Li, Kezhu Chen, Xiangyang Deng, Quan Zhang, Hailiang Tang, Zhifeng Shi, Tongming Zhu, Jianhong Zhu
Summary: Polystyrene nanoparticles (PS-NPs) are organic pollutants that pose potential threats to the environment and human health. This study investigates the neurotoxicity of PS-NPs and identifies the molecular mechanisms involving mitochondrial dysfunction and autophagy activation in human neuroblastoma SHSY-5Y cells.
Article
Clinical Neurology
Ruijie Geng, Haibin Li, Hao Wang, Chenyu Ye, Yemeng Mao, Xiao Huang
Summary: This study demonstrates that venlafaxine can alleviate neuronal apoptosis induced by hypoxia by upregulating the Wnt/beta-catenin signaling pathway, providing a new theoretical basis for the treatment of depression.
NEUROPSYCHIATRIC DISEASE AND TREATMENT
(2021)
Article
Toxicology
Nafila Pk, Ravi Kumar Rajan, Vanitha Nanchappan, Arjunan Karuppaiah, Jaikanth Chandrasekaran, Saravanan Jayaraman, Venkatesh Gunasekaran
Summary: C-Glucosyl Xanthone derivatives were evaluated for their inhibitory effects on the JNK3 mediated Caspase pathway in Almal-induced neurotoxicity in SHSY-5Y cells. Among them, Mangiferin showed neuroprotection by altering the architecture and increasing the density of neurons in SHSY-5Y cells. It exerted its protective effects by inhibiting JNK3 and down-regulating Caspase activation, improving apoptosis induced by Almal.
TOXICOLOGY MECHANISMS AND METHODS
(2023)
Article
Multidisciplinary Sciences
Anusha Dravid, Brad Raos, Darren Svirskis, Simon J. O'Carroll
Summary: Neuronal models are crucial in understanding neuroscientific processes, and adapting them to a high-throughput format allows for simultaneous screening of multiple agents. Differentiating SH-SY5Y cells is necessary to obtain mature neurons, but the absence of a standardized protocol hinders their use in high-throughput assays.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Hyunsoo Kim, Noriko Takegahara, Yongwon Choi
Summary: Protein phosphatase 2A (PP2A)-mediated dephosphorylation of glycogen synthase kinase-3 beta (GSK3β) plays a crucial role in Pcdh7-dependent activation of RhoA and osteoclast differentiation. Pcdh7-deficient cells show impaired PP2A activity, despite normal expression of PP2A. Inhibition of protein phosphatase and activation of PP2A can respectively decrease and rescue the dephosphorylation of GSK3β in Pcdh7(-/-) cells, affecting the activation of RhoA and osteoclast differentiation.
Article
Neurosciences
Margaret Hayne, Aaron DiAntonio
Summary: Protein phosphatase 2A (PP2A) is a cellular phosphatase that, when inhibited, disrupts multiple aspects of cellular physiology. This study demonstrates that loss of PP2A function leads to hyperphosphorylation of Tau and activation of the DLK pathway, causing neuronal cell death.
NEUROBIOLOGY OF DISEASE
(2022)
Article
Biology
Meysam Hassani Moghaddam, Neda Eskandari, Hossein Nikzad, Mohammad Miryounesi, Mohammad Karimian, Javad Amini Mahabadi, Mohammad Ali Atlasi
Summary: This study investigated the expression of genes associated with primordial germ cell development in differentiating mouse embryonic stem cells under the influence of progesterone and retinoic acid. It was found that RA treatment increased the expression of certain genes while decreasing others compared to the control group, and the combination of RA and progesterone (RA+P) further enhanced the differentiation process. Immunofluorescence studies showed higher expression levels in cells treated with RA+P, indicating a potential role for these factors in promoting germ cell differentiation.
JOURNAL OF BIOSCIENCES
(2021)
Article
Chemistry, Medicinal
Lailiang Qu, Shang Li, Limei Ji, Si Luo, Ming Ding, Fucheng Yin, Cheng Wang, Heng Luo, Dehua Lu, Xingchen Liu, Wan Peng, Lingyi Kong, Xiaobing Wang
Summary: Designing and synthesizing a series of highly potent GSK3 degraders, PT-65 has shown great potential in selectively inducing GSK3 degradation and ameliorating learning and memory impairments in an in vivo model of AD.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Phaewa Chaiwijit, Kwanchanok Uppakara, Nithi Asavapanumas, Witchuda Saengsawang
Summary: Mitochondria-associated membranes (MAMs) play a critical role in regulating cellular processes such as calcium homeostasis and mitochondrial function and dynamics. In Alzheimer's disease (AD), MAMs are upregulated, but the mechanisms behind this increase are still unclear. This study explores the correlation between protein phosphatase 2A (PP2A) and MAMs in neuronal-like cells. The inhibition of PP2A activity leads to increased MAM formation, disrupted mitochondrial function, and dynamics.
Article
Biochemistry & Molecular Biology
Zihan Qi, Ying Zhang, Kai Yao, Mengqi Zhang, Yixuan Xu, Jianfeng Zhang, Xiaojing Bai, Hengbing Zu
Summary: Downregulation of DHCR24 may be associated with the pathogenesis of AD, affecting tau protein phosphorylation and cholesterol synthesis, as demonstrated in a novel lipid raft-dependent PP2A signaling pathway.
NEUROCHEMICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Geoffrey Brown
Summary: All-trans retinoic acid plays a role in embryogenesis and also acts as a teratogen. The deregulation of cytosolic aldehyde dehydrogenases in various human cancers affects the synthesis of all-trans retinoic acid. Inhibiting these enzymes reduces cancer cell proliferation, induces apoptosis, and enhances chemotherapeutic sensitivity. RAR gamma is an oncogene in several types of cancer, and inhibiting its action leads to cancer cell death. Targeting the retinoic acid pathway shows promise in eliminating cancer stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Behavioral Sciences
Linda Marschner, An Schreurs, Benoit Lechat, Jesper Mogensen, Anton Roebroek, Tariq Ahmed, Detlef Balschun
BEHAVIOURAL BRAIN RESEARCH
(2019)
Article
Neurosciences
Antoine Leboucher, Tariq Ahmed, Emilie Caron, Anne Tailleux, Sylvie Raison, Aurelie Joly-Amado, Elodie Marciniak, Kevin Carvalho, Malika Hamdane, Kadiombo Bantubungi, Steve Lancel, Sabiha Eddarkaoui, Raphaelle Caillierez, Emmanuelle Vallez, Bart Staels, Didier Vieau, Detlef Balschun, Luc Buee, David Blum
NEUROBIOLOGY OF DISEASE
(2019)
Review
Neurosciences
Salam Salloum-Asfar, Noothan J. Satheesh, Sara A. Abdulla
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Multidisciplinary Sciences
Vijay Gupta, Safa Salim, Issam Hmila, Nishant N. Vaikath, Indulekha P. Sudhakaran, Simona S. Ghanem, Nour K. Majbour, Sara A. Abdulla, Mohamed M. Emara, Houari B. Abdesselem, Tamas Lukacsovich, Daniel Erskine, Omar M. A. El-Agnaf
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Nuha T. Swaidan, Salam Salloum-Asfar, Freshteh Palangi, Khaoula Errafii, Nada H. Soliman, Ahmed T. Aboughalia, Abdul Haseeb S. Wali, Sara A. Abdulla, Mohamed M. Emara
SCIENTIFIC REPORTS
(2020)
Review
Health Care Sciences & Services
Areej G. Mesleh, Sara A. Abdulla, Omar El-Agnaf
Summary: Autism spectrum disorder (ASD) is a multifactorial neurodevelopmental disorder characterized by impairments in social/communication skills and repetitive behavioral patterns. The prevalence of ASD has increased, with varying severity of symptoms and potential comorbidities. Discovering biomarkers is crucial for early screening of ASD.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Salam Salloum-Asfar, Ahmed K. Elsayed, Saba F. Elhag, Sara A. Abdulla
Summary: Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder diagnosed during early childhood, lacking reliable biomarkers. By identifying specific cir-ncRNAs in plasma, potential objective diagnostic biomarkers for ASD can be discovered, allowing for more targeted therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Ahmed K. Elsayed, Salam Salloum-Asfar, Sara A. Abdulla
Summary: A cell line, QBRIi013-A, derived from a 6-year-old female with ASD and intellectual disability, has been successfully generated using a non-integrating Sendai virus. This iPSC line exhibits pluripotency and differentiation potential, serving as a valuable cell model for understanding the molecular mechanisms underlying ASD pathogenesis.
STEM CELL RESEARCH
(2021)
Review
Virology
Noothan J. Satheesh, Salam Salloum-Asfar, Sara A. Abdulla
Summary: COVID-19 caused by SARS-CoV-2 affects the lungs and can enter the central nervous system, potentially leading to neurological complications such as encephalitis and encephalopathy. The virus may also trigger the development of Multiple Sclerosis (MS) as a secondary disease, similar to the increase in Parkinson's disease cases after the Spanish Flu in 1918.
Article
Cell & Tissue Engineering
Salam Salloum-Asfar, Rudolf Engelke, Hanaa Mousa, Neha Goswami, I. Richard Thompson, Freshteh Palangi, Kamal Kamal, Muna N. Al-Noubi, Frank Schmidt, Sara A. Abdulla, Mohamed M. Emara
Summary: This study investigated the response of human-induced pluripotent stem cells (hiPSCs) to hyperosmotic stress compared to non-pluripotent cell lines. It was found that hiPSCs exhibited a different pattern of stress granule (SG) formation under hyperosmotic conditions, which was independent of eIF2 alpha phosphorylation and associated with low apoptosis levels. Proteomics analysis revealed potential implications of microtubule organization on the response to hyperosmotic stress in hiPSCs, suggesting a cellular mechanism for understanding SG dynamics in pluripotent stem cells.
STEM CELLS INTERNATIONAL
(2021)
Article
Behavioral Sciences
Houda Yasmine Ali Moussa, Nimshitha Manaph, Gowher Ali, Selma Maacha, Kyung Chul Shin, Samia M. Ltaief, Vijay Gupta, Yongfeng Tong, Janarthanan Ponraj, Salam Salloum-Asfar, Said Mansour, Fouad A. Al-Shaban, Hyung-Goo Kim, Lawrence W. Stanton, Jean-Charles Grivel, Sara A. Abdulla, Abeer R. Al-Shammari, Yongsoo Park
Summary: Extracellular vesicles (EVs) are released from cells to facilitate cell-to-cell communication by transferring proteins and RNA. EVs, which can cross the blood-brain barrier, are considered as potential non-invasive biomarkers for neurological disorders. This study used flow cytometry to analyze individual neuronal EVs and found that the majority of EVs in plasma are CD63-positive exosomes of endosomal origin. Neural cell adhesion molecule (NCAM), a neuronal EV marker, was highly enriched in EVs released from induced pluripotent stem cells-derived cortical neurons and brain organoids. Plasma NCAM-positive neuronal EVs can be used to discover biomarkers for neurological disorders.
FRONTIERS IN INTEGRATIVE NEUROSCIENCE
(2022)
Article
Cell Biology
Salam Salloum-Asfar, Sara A. A. Abdulla, Rowaida Z. Z. Taha, I. Richard Thompson, Mohamed M. M. Emara
Summary: Somatic cells can be reprogrammed to iPSCs using reprogramming factors, and the addition of miRNAs has been shown to increase iPSC formation. This study identified potential regulomics and regulatory networks of miRNA-mRNA interactions and other ncRNAs in enhancing iPSC generation, pluripotency, and differentiation.
Article
Biochemistry & Molecular Biology
Areej Mesleh, Hanan Ehtewish, Alberto de la Fuente, Hawra Al-shamari, Iman Ghazal, Fatema Al-Faraj, Fouad Al-Shaban, Houari B. Abdesselem, Mohamed Emara, Nehad M. Alajez, Abdelilah Arredouani, Julie Decock, Omar Albagha, Lawrence W. Stanton, Sara A. Abdulla, Omar M. A. El-Agnaf
Summary: Autism spectrum disorder (ASD) is a group of neurodevelopmental disorders characterized by impairments in social and communication skills, along with repetitive and restrictive behaviors or interests. Currently, there are no approved biomarkers for ASD diagnosis, and the diagnosis heavily relies on physician's assessment and awareness of symptoms. This study identified potential blood proteomic biomarkers for ASD using proximity extension assay (PEA) and machine learning analysis. Dysregulation of SNARE vesicular transport and ErbB pathways were found in ASD cases, and proteins from these pathways were correlated with ASD severity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Integrative & Complementary Medicine
Mustafa T. Ardah, Simona S. Ghanem, Sara A. Abdulla, Guohua Lv, Mohamed M. Emara, Katerina E. Paleologou, Nishant N. Vaikath, Jia-Hong Lu, Min Li, Konstantinos Vekrellis, David Eliezer, Omar M. A. El-Agnar
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2020)