Article
Cell Biology
Setsuko Komatsu, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida
Summary: The mixture of silver nanoparticles, nicotinate, and potassium nitrate was found to have positive effects on wheat seedlings by regulating pathogen-related protein and reactive-oxygen species scavenging. Additionally, increasing starch content and decreasing lipoxygenase could promote wheat growth.
Article
Chemistry, Physical
Anna Gorczyca, Ewa Pociecha, Julia Maciejewska-Pro, Monika Kula-Maximenko, Magdalena Ocwieja
Summary: This study investigated the phytotoxic effects of silver nanoparticles (AgNPs) with different surface properties on wheat plants. The results showed that AgNPs stabilized with sodium hexametaphosphate exhibited the highest toxicity, while citrate-stabilized AgNPs showed the least toxicity to the seedlings.
SURFACE INNOVATIONS
(2022)
Article
Environmental Sciences
N. Federico Spagnoletti, Florencia Kronberg, Cecilia Spedalieri, Eliana Munarriz, Romina Giacometti
Summary: The development of environmentally friendly procedures for synthesizing metallic nanoparticles using proteins and enzymes from plants, fungi, yeast, and bacteria is a valuable and efficient alternative to conventional methods. This study compares biogenic silver nanoparticles from the fungus Macrophomina phaseolina to chemically synthesized nanoparticles in terms of their biocidal activity and toxicity. The research shows that bio-AgNPs have similar bactericidal activity to chemical AgNPs but exhibit lower toxicity in the organism Caenorhabditis elegans. Biochemical and proteomic techniques were used to identify the proteins involved in the synthesis and stability of the bio-AgNPs, suggesting that these proteins play a role in preserving the silver core and protecting eukaryotic cells from metal toxicity.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Agronomy
Ewa Pociecha, Anna Gorczyca, Michal Dziurka, Ewelina Matras, Magdalena Ocwieja
Summary: The study confirmed that using AgNPs stabilized by specific compounds can promote wheat growth and reduce flowering time, while silver ions only increase plant height without affecting flowering time. Accelerated flowering was caused by changes in plant hormone balance, especially GA6.
Article
Engineering, Environmental
Tin Yan Wong, Neng Yan, Kenneth Kin Leung Kwan, Yanrong Pan, Jingjing Liu, Yao Xiao, Long Wu, Henry Lam
Summary: Silver nanoparticles (AgNPs) have raised concerns due to their potential harmful effects on human beings. This study investigated the hepatotoxic effects of AgNPs using a comparative proteomics approach. The results showed that cancerous liver cells and normal cells respond to AgNPs exposure through different pathways, providing new insights into mechanisms underlying AgNPs-induced hepatotoxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Rekha Kannaujia, Pratiksha Singh, Vivek Prasad, Vivek Pandey
Summary: The study evaluated the potential of biogenic silver nanoparticles (B-AgNPs) as an effective ozone protectant for wheat, showing promising results in enhancing ozone tolerance and improving yield attributes in wheat cultivars. Particularly, the 25 mg/L concentration of B-AgNPs demonstrated a more pronounced effect on yield attributes and lower accumulation in grains for both cultivars.
ENVIRONMENTAL RESEARCH
(2022)
Article
Plant Sciences
Leslaw B. Lahuta, Joanna Szablinska-Piernik, Karolina Stalanowska, Marcin Horbowicz, Ryszard J. Gorecki, Viorica Railean, Pawel Pomastowski, Boguslaw Buszewski
Summary: Plant responses to stresses caused by metal nanoparticles are affected by cyclitols, which are absorbed and transported within the plant. Cyclitols induce the accumulation of sucrose and 1-kestose in seedlings, while (Bio)Ag NPs increase the concentrations of these sugars. Additionally, cyclitols decrease the concentrations of fructose and glucose. Similar effects were observed in seedlings developed from primed grains.
Article
Agronomy
Abdul Manaf, Xiukang Wang, Fatima Tariq, Hafiz Muhammad Jhanzab, Yamin Bibi, Ahmad Sher, Abdul Razzaq, Sajid Fiaz, Sikander Khan Tanveer, Abdul Qayyum
Summary: The application of silver and gold nanoparticles significantly enhanced the antioxidant enzyme activities, leading to increased growth parameters in wheat production, suggesting the potential of nanotechnology to promote crop growth.
Review
Plant Sciences
Deepmala Sehgal, Priyanka Dhakate, Heena Ambreen, Khasim Hussain Baji Shaik, Nagenahalli Dharmegowda Rathan, Nayanahalli Munireddy Anusha, Rupesh Deshmukh, Prashant Vikram
Summary: Plant omics has played a crucial role in discovering new genes and biomolecules that can improve crops, and has made significant progress in wheat research on both qualitative and quantitative traits. Combining various omics approaches has led to advances in gene discovery, pathway investigation, and understanding stress responses and yields. The development of a Wheat Omics database further aids functional genomics studies in wheat.
Article
Biochemistry & Molecular Biology
Leslaw Bernard Lahuta, Joanna Szablinska-Piernik, Karolina Stalanowska, Katarzyna Glowacka, Marcin Horbowicz
Summary: The phytotoxicity of silver nanoparticles (Ag NPs) to plant seeds and seedlings depends on the properties and concentration of the nanoparticles, as well as the species and stress tolerance of the plants. In this study, citrate-stabilized spherical Ag NPs of different sizes were found to have no effect on the germination of wheat grains, but smaller nanoparticles decreased the growth of seedling roots. Significant rearrangements in carbohydrate profiles were observed in the seedlings, while most other polar metabolites remained unaffected.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Leslaw Bernard Lahuta, Joanna Szablinska-Piernik, Katarzyna Glowacka, Karolina Stalanowska, Viorica Railean-Plugaru, Marcin Horbowicz, Pawel Pomastowski, Boguslaw Buszewski
Summary: This study analyzed the effects of bio-synthesized silver nanoparticles on wheat germination, early seedling development, and metabolic profiles of different tissues. It found that silver nanoparticles inhibited the growth of coleoptile and affected the concentration of metabolites in roots, coleoptile, and endosperm.
Article
Chemistry, Applied
Jiawen Pei, Jie Bai, Liangjie Tian, Dandan Xiong, Chunxia Xiao, Xuebo Liu, Huaide Xu, Xiang Duan
Summary: In this study, a co soluble system (WG-PSV complex) was successfully established to improve the solubility of wheat gluten (WG) through pH-shifting treatment. The complex exhibited smaller particles, higher Zeta potential, and a more flexible secondary structure compared to native WG. Additionally, key subunits related to solubility improvement were identified. This study provides a theoretical foundation for enhancing the solubility of WG and expanding its applications in the food industry.
FOOD HYDROCOLLOIDS
(2024)
Article
Biochemistry & Molecular Biology
Delphine Vincent, AnhDuyen Bui, Doris Ram, Vilnis Ezernieks, Frank Bedon, Joe Panozzo, Pankaj Maharjan, Simone Rochfort, Hans Daetwyler, Matthew Hayden
Summary: This study optimized a LC-MS shotgun quantitative proteomics method to screen wheat genotypes for breeding lines with better performance. A large number of wheat proteins were identified using this method, and data mining tools were used to explore the flour proteome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Chong-Yang Xing, Teng-Fei Ma, Jin-Song Guo, Yu Shen, Peng Yan, Fang Fang, You-Peng Chen
Summary: Proteinaceous nanocompartments were shown to effectively remove silver from water and enhance bacterial resistance to toxic silver by reducing intracellular silver concentration, providing a potential bioremediation pathway for heavy metal pollutants in water.
Review
Biochemistry & Molecular Biology
David Kovacs, Nora Igaz, Mohana K. Gopisetty, Monika Kiricsi
Summary: This review highlights the potential of silver nanoparticles in cancer treatment, including their cancer-specific targeting, reduced side effects, and strong anti-cancer properties. It also discusses the critical hurdles in their clinical utilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Madoka Ikemoto-Uezumi, Heying Zhou, Tamaki Kurosawa, Yuki Yoshimoto, Masashi Toyoda, Nobuo Kanazawa, Tatsu Nakazawa, Mitsuhiro Morita, Kunihiro Tsuchida, Akiyoshi Uezumi
Summary: The study reveals an association between increased MFG-E8 levels in skeletal muscle and sarcopenia, suggesting that MFG-E8 may contribute to the degeneration of neuromuscular junctions. Targeting MFG-E8 could be a promising therapeutic approach to prevent sarcopenia.
Article
Biochemistry & Molecular Biology
Keisuke Hitachi, Yuri Kiyofuji, Masashi Nakatani, Kunihiro Tsuchida
Summary: This study reveals the multiple inhibitory functions of heterogeneous nuclear ribonucleoprotein K (hnRNPK) during myogenic differentiation, indicating the importance of analyzing lncRNA-binding proteins for understanding the physiological functions of lncRNAs and the multiple functions of RBPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Kentaro Takayama, Keisuke Hitachi, Hideyuki Okamoto, Mariko Saitoh, Miki Odagiri, Rina Ohfusa, Takahiro Shimada, Akihiro Taguchi, Atsuhiko Taniguchi, Kunihiro Tsuchida, Yoshio Hayashi
Summary: A new myostatin inhibitory peptide, MID-35, has been developed with improved stability and resistance to biodegradation. It significantly increases the tibialis anterior muscle mass in mice and can be used for prolonged inactivation of myostatin in skeletal muscle.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Cell Biology
Setsuko Komatsu, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida
Summary: The mixture of silver nanoparticles, nicotinate, and potassium nitrate was found to have positive effects on wheat seedlings by regulating pathogen-related protein and reactive-oxygen species scavenging. Additionally, increasing starch content and decreasing lipoxygenase could promote wheat growth.
Article
Oncology
Haitao Gu, Wensheng Deng, Yi Zhang, Yu Chang, Vishal G. Shelat, Kunihiro Tsuchida, Leonardo S. Lino-Silva, Zhaowen Wang
Summary: In this study, we found that NLRP3 plays an important role in the polarization of macrophages in lung metastasis of pancreatic ductal adenocarcinoma (PDAC). NLRP3 activation in TAMs enhances the lung metastasis of PDAC.
TRANSLATIONAL LUNG CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Setsuko Komatsu, Kazuki Murata, Sayuri Yakeishi, Kazuyuki Shimada, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Rumina Obi, Shoichi Akita, Ryo Fukuda
Summary: Fiber crosslinked with zinc oxide nanoparticles (NPs) was found to enhance soybean growth. Compared to the solution form of ZnO NPs, fiber crosslinked with ZnO NPs promoted soybean growth at a lower concentration. Proteomic analysis revealed that proteins involved in photosynthesis and secondary metabolism accumulated more in soybeans grown on fiber crosslinked with ZnO NPs. Additionally, the abundance of NADPH oxidoreductase, which increased in soybeans by ZnO NPs application, might be related to the growth-promoting effect of auxin with fiber crosslinked with ZnO NPs on soybean growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Ghazala Mustafa, Suzuna Miyagawa, Murtaza Hasan, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Setsuko Komatsu
Summary: Plant-mediated synthesis methods of nanoflowers (NFs) enable the synthesis of stable and economical zinc oxide (ZnO) NFs, which have a positive impact on soybean growth. Bio-synthesized (BS) NFs ZnO can enhance root length and weight, regulate hormone metabolism, improve protein folding, and promote redox metabolism through detoxification of hydrogen peroxide, compared to chemically synthesized nanowires (CS NWs) ZnO.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Keisuke Hitachi, Yuri Kiyofuji, Hisateru Yamaguchi, Masashi Nakatani, Masafumi Inui, Kunihiro Tsuchida
Summary: This study investigated the effects of the loss of MyHC-IIb and MyHC-IIx on skeletal muscle function using a mouse model. The results showed that the simultaneous loss of IIb and IIx led to severe muscle hypoplasia after 2 weeks of age in mice. Increased expression of other MyHCs was observed in 3-week-old mice, but it was not sufficient to compensate for the loss of IIb and IIx. Aberrant sarcomere structure and altered expression of sarcomere components were also observed. These findings contribute to the understanding of molecular mechanisms underlying skeletal muscle wasting disorders caused by the loss of skeletal muscle MyHCs.
Article
Multidisciplinary Sciences
Masahide Harada, Daisuke Okuzaki, Akemi Yamauchi, Shiho Ishikawa, Yoshihiro Nomura, Asuka Nishimura, Yuji Motoike, Masayuki Koshikawa, Keisuke Hitachi, Kunihiro Tsuchida, Kentaro Amano, Atsuo Maekawa, Yasushi Takagi, Eiichi Watanabe, Yukio Ozaki, Hideo Izawa
Summary: This study aimed to identify cardiac-specific circulating miRNAs as biomarkers for atrial fibrillation (AF). The researchers found that miR-20b-5p and miR-330-3p can serve as cardiac-specific biomarkers for atrial remodeling progression and arrhythmia recurrence after catheter ablation in AF patients.
Article
Endocrinology & Metabolism
Shinji Ueno, Yusuke Seino, Shihomi Hidaka, Masashi Nakatani, Keisuke Hitachi, Naoya Murao, Yasuhiro Maeda, Haruki Fujisawa, Megumi Shibata, Takeshi Takayanagi, Katsumi Iizuka, Daisuke Yabe, Yoshihisa Sugimura, Kunihiro Tsuchida, Yoshitaka Hayashi, Atsushi Suzuki
Summary: This study investigated the effect of blockade of glucagon action on skeletal muscle using GCGKO mice. The results showed that blockade of glucagon action increased skeletal muscle weight and stimulated slow-to-fast transition in type II fibers, resembling the phenotype of a high-protein diet.
JOURNAL OF DIABETES INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Tadahiro Nagaoka, Tatsuya Katsuno, Kyoka Fujimura, Kunihiro Tsuchida, Masashi Kishi
Summary: Here, we provide genetic and molecular evidence of a functional interaction between Vangl2 and N-cadherin for PCP-dependent neural development. This interaction is essential for planar polarized development of neural tissues but is not significantly associated with the RhoA or JNK pathways of Wnt/PCP signaling.
SCIENTIFIC REPORTS
(2023)
