Journal
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Volume 43, Issue 4, Pages 1559-1574Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2019.4112
Keywords
CRISPR/Cas9; genome editing; disease models; pulmonary disease; gastrointestinal disease; hematologic disease; viral disease; cancer; autoimmune disease; inflammatory disease
Categories
Funding
- Tecnologico de Monterrey and Mission XXI
Ask authors/readers for more resources
Genome editing reemerged in 2012 with the development of CRISPR/Cas9 technology, which is a genetic manipulation tool derived from the defense system of certain bacteria against viruses and plasmids. This method is easy to apply and has been used in a wide variety of experimental models, including cell lines, laboratory animals, plants, and even in human clinical trials. The CRISPR/Cas9 system consists of directing the Cas9 nuclease to create a site-directed double-strand DNA break using a small RNA molecule as a guide. A process that allows a permanent modification of the genomic target sequence can repair the damage caused to DNA. In the present study, the basic principles of the CRISPR/Cas9 system are reviewed, as well as the strategies and modifications of the enzyme Cas9 to eliminate the off-target cuts, and the different applications of CRISPR/Cas9 as a system for visualization and gene expression activation or suppression. In addition, the review emphasizes on the potential application of this system in the treatment of different diseases, such as pulmonary, gastrointestinal, hematologic, immune system, viral, autoimmune and inflammatory diseases, and cancer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available