Article
Thermodynamics
Rui Chen, Jialiang Zhou, Xin Zheng, Lingwei Jiang, Na Duan
Summary: The synergy in anaerobic co-digestion of cattle manure and Chlorella sp. under high feeding loads was studied. AcoD enhanced the methane yield of both feedstocks compared to mono-digestion. The protein/carbohydrates ratio was identified as an important factor for the synergy. AcoD at a ratio of 8:2 showed the highest methane yield, fastest methane production rate, and high abundance of Methanosaeta.
Article
Chemistry, Physical
Sabarathinam Shanmugam, Thangavel Mathimani, Eldon R. Rene, V. Edwin Geo, A. Arun, Kathirvel Brindhadevi, Arivalagan Pugazhendhi
Summary: Biohythane, a blend of hydrogen with methane, is produced through the breakdown of organic substrates using sequential dark fermentation and anaerobic digestion. Improving its production rate and quality can be achieved by altering microbial consortium, substrate selection, reactor design, and implementing a two-stage fermentation system. Comparative studies show the superiority of biohythane over other biofuels.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Review
Agricultural Engineering
Prawat Sukphun, Worapong Wongarmat, Tsuyoshi Imai, Sureewan Sittijunda, Sumate Chaiprapat, Alissara Reungsang
Summary: The transition to renewable energy sources is essential for a sustainable future. This review focuses on the potential of two-stage anaerobic digestion (TSAD) for utilizing waste from the sugar and ethanol industries, specifically for biohydrogen and methane production. Various factors affecting TSAD process performance are discussed, along with its ability to reduce greenhouse gas emissions. The review also highlights recent findings and promising future research related to TSAD, including advanced approaches like meta-omics, gene manipulation, and bioaugmentation.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sadib Bin Kabir, Md Khalekuzzaman, Nazia Hossain, Mamun Jamal, Md Asraful Alam, Abd El-Fatah Abomohra
Summary: Recent advances in microalgae to biohythane conversion using co-digestion with wastewater sludge have been reviewed. This approach provides solutions to overcome the challenges of microalgae-based biohythane production and offers a sustainable future.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Agricultural Engineering
Md Tabish Noori, Booki Min
Summary: This review provides a detailed analysis of recently discovered mechanisms and strategies to improve the composition and production rate of biohythane. It also includes a future perspective on novel research lines to enhance reactor performance.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Hong Chen, Tao Yang, Zhiqiang Shen, Enzhe Yang, Ke Liu, Hong Wang, Jing Chen, Eli Hendrik Sanjaya, Sha Wu
Summary: Digestate recirculation is important for improving system stability and gas production performance. However, it does not promote biohythane production in anaerobic co-digestion. Digestate recirculation has positive effects on removal rates and energy yield. There is a high abundance of hydrogen-producing bacteria under thermophilic conditions, which has significant hydrogen production potential.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Agricultural Engineering
Hoo Hugo Kim, Shouvik Saha, Jae-Hoon Hwang, Md. Aoulad Hosen, Yong -Tae Ahn, Young -Kwon Park, Moonis Ali Khan, Byong-Hun Jeon
Summary: This review provides an overview of the feasibility of biohythane production and discusses possible technologies for upgrading it to hydrogen-enriched renewable natural gas. It also highlights the crucial role of meta-omics and metabolic engineering approaches in biohythane production technology.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Hong Chen, Rong Huang, Jun Wu, Wenzhe Zhang, Yunping Han, Benyi Xiao, Dongbo Wang, Yaoyu Zhou, Bing Liu, Guanlong Yu
Summary: Biohythane production was successfully achieved through temperature-phased anaerobic co-digestion of rice straw and pig manure, with effective selective pressure for hydrogen-consuming microbes during the thermophilic acid-producing phase. However, hydrolysis could be a rate-limiting step in the process.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
F. Yilmaz, E. Kokdemir Unsar, N. A. Perendeci, E. Sahinkaya
Summary: The study demonstrates that co-digestion of aniseed with vinasse has a positive impact on methane production, and adding vinasse as a co-substrate can improve the methane production of pomace. Utilizing a ratio of A:V:P = 33:33:33 for co-digestion provides a suitable option for maximizing waste utilization and methane production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Energy & Fuels
Mohammad Taghi Samadi, Mostafa Leili, Alireza Rahmani, Samira Moradi, Kazem Godini
Summary: The aim of this study was to determine the feasibility of producing biogas by combining solid poultry slaughterhouse wastes with fruit and vegetable solid wastes and to obtain the optimum conditions for maximum biogas production. The study found that the highest biogas yield occurred when the C/N ratio of the feed was 30.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Review
Environmental Sciences
Ashutosh Kumar Pandey, S. Pilli, P. Bhunia, R. D. Tyagi, Y. Rao Surampalli, C. Tian Zhang, Sang-Hyoun Kim, Ashok Pandey
Summary: VFAs are the building blocks of the chemical industry and play a key role in the planet's organic carbon cycle. To address the issues of unsustainable VFA production from fossil fuels, it is crucial to develop alternate sources through anaerobic digestion. By researching accessible feedstocks, influencing parameters, and utilizing VFAs to create consumer products, economic stability can be achieved through traditional and advanced production methods.
Article
Biotechnology & Applied Microbiology
Sen Wang, Qiang Zhao, Haiyang Yu, Xinxin Du, Ting Zhang, Tongxin Sun, Wenlu Song
Summary: Digestates from different anaerobic digesters have potential as substrates for microalgal culture, allowing effective wastewater treatment and microalgal biomass production. However, further research is needed before large-scale application. This study investigated the culture of Chlorella sp. in Digestate(M) from anaerobic fermentation of brewer's grains and brewery wastewater (BWW) and explored the use of the biomass under different experimental conditions. The results showed that cultivation in Digestate(M) with 10% loading and 20% BWW achieved the highest biomass production and demonstrated effective remediation of various pollutants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Energy & Fuels
Young-Ju Song, Kyung-Su Oh, Beom Lee, Dae-Won Pak, Ji-Hwan Cha, Jun-Gyu Park
Summary: This study determined the optimal mixing ratio of food waste and livestock manure for efficient co-digestion of sewage sludge by applying the BMP test and software simulation. The results showed that a higher mixing ratio of food waste led to a higher methane yield and improved biodegradability. Continuous reactor operation under the optimal mixing ratio resulted in greater organic matter removal and methane yield, improving process stability compared to operations with individual substrates.
Article
Biotechnology & Applied Microbiology
Napapat Sitthikitpanya, Sureewan Sittijunda, Sontaya Khamtib, Alissara Reungsang
Summary: This study demonstrates a successful integrated circular biorefinery platform for efficiently utilizing Chlorella sp. biomass and sugarcane leaves to produce hydrogen, methane, PHA, and lipid. The solid residues and acidified slurries at the end of hydrogen and methane production can be used as soil supplements, showcasing the zero waste concept. The approach presented in this study lays the foundation for optimal feedstock utilization, resulting in zero waste generation.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Environmental Sciences
Hu Yan, Fanfan Cai, Ligong Wang, Chang Chen, Guangqing Liu
Summary: This study conducted a systematic analysis of the composition and biomethane potential of 20 typical vegetable wastes (VWs), showing a correlation between organic components and methane production for rapid prediction. Furthermore, different models were used to describe the biochemical methanogenesis mechanism of these VWs. The results can provide a reference for fundamental research on the anaerobic digestion of VW and serve as a method to predict methane production by analyzing compositional components, contributing to pollution prevention and comprehensive utilization of VW in the future.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Engineering, Environmental
Khaliyah Sani, Prawit Kongjan, Charoen Pakhathirathien, Benjamas Cheirsilp, Sompong O-Thong, Marisa Raketh, Rusnee Kana, Rattana Jariyaboon
Summary: The study investigated the biohydrogen and biomethane potential of palm oil mill effluent (POME) in a two-stage anaerobic digestion system under thermophilic and mesophilic conditions. The results showed that the best production potentials were obtained from POME with an initial 0.5 g-COD loading, and the energy yield achieved from two-stage AD was significantly higher than single-stage AD. Continuous production of hydrogen and methane revealed that the highest yields were generated at specific hydraulic retention times, with an overall COD removal of approximately 66.27%.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Agricultural Engineering
Chonticha Mamimin, Sukonlarat Chanthong, Chonticha Leamdum, Sompong O-Thong, Poonsuk Prasertsan
Summary: Bio-pretreatment of EFB with straw mushroom cultivation improves biodegradability and methane production, making it a promising strategy for achieving an environmentally friendly and economically feasible process.
BIORESOURCE TECHNOLOGY
(2021)
Article
Energy & Fuels
Khandaker Dahirul Islam, Thanansak Theppaya, Fida Ali, Jompob Waewsak, Tanita Suepa, Juntakan Taweekun, Teerawet Titseesang, Kuaanan Techato
Summary: Diversifying the energy mix of Bangladesh by exploring wind energy potential in its southern coastal areas is crucial for improving energy security and reducing carbon emissions. The study identified promising sites with annual mean wind speeds of 7.3 m/s, such as Charfashion and Monpura, for energy generation.
Article
Microbiology
Srisuda Chaikitkaew, Supattra In-Chan, Apinya Singkhala, Wisarut Tukanghan, Chonticha Mamimin, Alissara Reungsang, Nils-Kare Birkeland, Sompong O-Thong
Summary: A new strain PL3(T) of the genus Clostridium, capable of producing acetate, was isolated and characterized in this study. The strain exhibited wide growth conditions and diverse metabolic capabilities. Phylogenetic analysis and DNA-DNA hybridization confirmed that PL3(T) represents a novel species in the genus Clostridium.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kittisak Jantanasakulwong, Sarinthip Thanakkasaranee, Phisit Seesuriyachan, Pisith Singjai, Aphisit Saenjaiban, Siriphan Photphroet, Kanticha Pratinthong, Yuthana Phimolsiripol, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Sommano, Pensak Jantrawut, Siriwadee Chomdej, Suwit Chotinan, Francisco J. Barba, Joe M. Regenstein, Alissara Reungsang, Pornchai Rachtanapun
Summary: The nano-metal-treated PET films with anti-virus and anti-fogging ability were developed using sparking nano-metal particles of Ag, Zn, and Ti wires on polyethylene terephthalate (PET) films. A highly transparent, anti-fogging, and anti-virus face shield was prepared using the Zn-PET film. Sparking was an effective technique to prepare the alternative anti-virus and anti-fogging films for medical biomaterial applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kamolwan Thepsuthammarat, Alissara Reungsang, Pensri Plangklang
Summary: Improving biomass production by using low-cost substrates is important for large-scale microalgae production. In this study, the microalga Coelastrella sp. KKU-P1 was cultivated mixotrophically using unhydrolyzed molasses as a carbon source. The optimal conditions for maximizing biomass production were determined, and a batch cultivation achieved the highest biomass production of 3.81 g/L. CO2 supplementation did not improve biomass production, indicating that ambient CO2 concentration was sufficient for mixotrophic growth. The biochemical analysis showed that the microalgal biomass has potential as a source of essential amino acids, pigments, and fatty acids.
Article
Environmental Sciences
Marisa Raketh, Rusnee Kana, Prawit Kongjan, Syed Anuar Faua'ad Muhammad, Sompong O-Thong, Chonticha Mamimin, Rattana Jariyaboon
Summary: This study investigates the biohydrogen and biomethane potential of co-digestion from palm oil mill effluent (POME) and concentrated latex wastewater (CLW). It finds that specific mixing ratios can achieve the highest yields of hydrogen and methane, and continuous reactors also produce high amounts of biogas.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Biotechnology & Applied Microbiology
Rattana Jariyaboon, Surananee Hayeeyunu, Nikannapas Usmanbaha, Shahrul Bin Ismail, Sompong O-Thong, Chonticha Mamimin, Prawit Kongjan
Summary: Food waste, a type of organic solid waste, has a negative impact on environmental sustainability. Dark fermentation was used to simultaneously generate mixed volatile fatty acids (VFAs) and bio-hydrogen from food waste. The study found that the pretreated inoculum was capable of converting 10 g VS/L food waste to hydrogen with a high yield of 135.2 ± 7 mL H-2/VSadded.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Gede Adi Wiguna Sudiartha, Tsuyoshi Imai, Chonticha Mamimin, Alissara Reungsang
Summary: Temperature plays a significant role in anaerobic digestion, affecting microbial communities and process efficiency. This study tested two temperature shift scenarios and found that both led to a decrease in biogas production, with the upshifted scenario experiencing a larger decrease. 16S rRNA sequencing revealed that the dominant methanogens differed between the two scenarios, indicating that temperature shifts affect microbial communities and biogas production.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Sureewan Sittijunda, Sulfan Baka, Rattana Jariyaboon, Alissara Reungsang, Tsuyoshi Imai, Prawit Kongjan
Summary: This study aimed to enhance dark fermentative hydrogen production from co-digestion of distillery wastewater and glycerol waste. The optimal ratio of DW and GW for hydrogen production was found to be 99:1, resulting in a hydrogen yield of 149.5 mL-H-2/g - VSadded. The dark fermentation effluent was then used for methane production, with a maximum methane yield of 115.1 mL-CH4/g - VSadded. Microbial community analysis revealed the presence of methanogenic archaea in the microbial electrolysis cell reactor.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Marika Ngamsirisomsakul, Mallika Boonmee Kongkeitkajorn, Sittipong Amnuaypanich, Alissara Reungsang
Summary: Sugarcane bagasse can be used as a raw material for microbial lipid production, and incorporating glycerol into unconcentrated bagasse hydrolysate helps improve growth and lipid production in Rhodotorula glutinis TISTR 5159. When mixed with 10% v/v glycerol, the hydrolysate with yeast extract as the sole nitrogen source achieved the highest lipid yield.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Khamanitjaree Saripan, Chonticha Mamimin, Tsuyoshi Imai, Sureewan Sittijunda, Alissara Reungsang
Summary: This study aims to create a q-PCR methodology for monitoring microbial communities in hydrogen-producing mixed cultures and develop specific primers for quantitative analysis of hydrogen-producing bacteria. The dominant bacteria in the enriched thermophilic mixed cultures were identified as Clostridium sp. and Thermoanaerobacterium sp., and their 16S rRNA gene copy number increased with time during hydrogen production.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Apik Khautsart Miftah, Sureewan Sittijunda, Tsuyoshi Imai, Apilak Salakkam, Alissara Reungsang
Summary: This study determined the optimal conditions for the pretreatment of sugarcane leaves with deep eutectic solvent (DES), and found the best fermentation mode for hydrogen and methane production from DES-pretreated sugarcane leaves. The results showed that the simultaneous saccharification and fermentation (SSF) process was more efficient than the separate hydrolysis and fermentation (SHF) process in both two-stage hydrogen and methane production process and one-stage methane production process.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Sreyden Hor, Mallika Boonmee Kongkeitkajorn, Alissara Reungsang
Summary: This study investigates the biorefinery of sugarcane bagasse into ethanol and xylitol. The results show that ethanol fermentation of sugarcane bagasse hydrolysate can be carried out without supplementing a nitrogen source, and approximately 50 g/L of bioethanol can be produced after 36 hours of fermentation. The vinasse containing xylose can be successfully used to produce xylitol, and supplementing the vinasse with yeast extract improves xylitol production. Controlled dissolved oxygen and the addition of low fraction of molasses do not positively affect xylitol production.
FERMENTATION-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Pornchai Rachtanapun, Sarinthip Thanakkasaranee, Rafael A. Auras, Nareekan Chaiwong, Kittisak Jantanasakulwong, Pensak Jantrawut, Yuthana Phimolsiripol, Phisit Seesuriyachan, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Somman, Warintorn Ruksiriwanich, Warinporn Klunklin, Alissara Reungsang, Thi Minh Phuong Ngo
Summary: The effect of sodium hydroxide concentrations on the morphology, mechanical properties, and water barrier properties of carboxymethyl rice starch films was investigated. Increasing NaOH concentrations resulted in higher degree of substitution of CMSr powders, leading to changes in morphology. The water solubility, elongation at break, and water vapor permeability of the films increased as NaOH concentrations increased, while the water contact angle, melting temperature, and tensile strength decreased.
