Article
Chemistry, Physical
Doan-Thanh Ta, Chiu-Yue Lin, Thi-Minh-Ngoc Ta, Chen-Yeon Chu
Summary: A novel single-reactor system has been developed for co-production of H2 and CH4. The study shows that a short pH shock can regulate the H2/CH4 composition without significantly affecting biogas yield and COD removal.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Agricultural Engineering
Mahmoud M. Ali, Ahmed M. Mustafa, Ximing Zhang, Hongjian Lin, Xin Zhang, Umar Abdulbaki Danhassan, Xuefei Zhou, Kuichuan Sheng
Summary: The addition of molybdate and ferric chloride to the dark fermentation stage improves biohythane production from sulfate-rich hydrolyzed tofu processing residue and reduces H2S production.
BIORESOURCE TECHNOLOGY
(2022)
Review
Energy & Fuels
K. B. Sasidhar, P. Senthil Kumar, Leilei Xiao
Summary: With the increasing concern over global warming, a growing number of countries have joined the net-zero coalition to reduce fossil fuel consumption. Additionally, there is a strong focus on reducing the pollution load from waste biomass. Sequential biohydrogen and biomethane production has been found to offer operational stability, higher energy yield, and cleaner by-products. This review provides insights and discusses the challenges of scaling up biohydrogen and biomethane production, as well as the viability of Hythane as a clean and renewable fuel with over 40% reduction in carbon monoxide and carbon dioxide emissions.
Article
Chemistry, Physical
Mai-Linh Thi Nguyen, Pang-Chun Hung, Tan-Phat Vo, Chyi-How Lay, Chiu-Yue Lin
Summary: The study investigated the production of mesophilic biohythane gas in a bioreactor via single-stage dark fermentation, showing that the F/M ratio and fermentation time affect biohythane production efficiency. The maximum hydrogen production rate was 9.60 L/L-d, maximum methane production rate was 0.72 L/ L-d.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Energy & Fuels
Mahmoud M. Ali, Ahmed M. Mustafa, Ximing Zhang, Xin Zhang, Umar Abdulbaki Danhassan, Hongjian Lin, Ungyong Choe, Kuichuan Sheng, Kaiying Wang
Summary: This study demonstrated that two-stage anaerobic digestion (TAD) is an efficient technology for producing biohythane and value-added products using tofu processing residue (TPR). Optimal conditions for TAD were found to be dark fermentation followed by methanogenic fermentation. TAD improved energy recovery and influenced microbial community diversity.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Multidisciplinary Sciences
Zhenli Diao, Dongsheng Han, Rui Zhang, Jinming Li
Summary: This review summarizes the potential applications and challenges of metagenomic next-generation sequencing (mNGS) in the diagnosis of lower respiratory tract infections (LRIs), aiming to promote the integration of mNGS into the management of patients with respiratory tract infections in a clinical setting.
JOURNAL OF ADVANCED RESEARCH
(2022)
Review
Biology
Haeun Lee, Jun Won Min, Seyoung Mun, Kyudong Han
Summary: This review discusses the recently studied and utilized NGS technologies and effective computational approaches for discovering retrotransposons. NGS has provided new perspectives for detecting retrotransposon-mediated SVs and various computational methods have been developed for accurately detecting insertions and deletions in the human genome.
Article
Chemistry, Physical
Tan-Trung Nguyen, Doan-Thanh Ta, Chiu-Yue Lin, Chen-Yeon Chu, Thi-Minh-Ngoc Ta
Summary: This study examined the production performance and composition of biohythane under different hydraulic retention time conditions using a mixture of swine manure and pineapple waste in a newly-developed single-stage anaerobic fermentation system. The results showed that a hydraulic retention time of 6 hours achieved the peak gas production performance with the highest hydrogen and methane production rates. Acetate and butyrate were identified as the dominant liquid metabolites in the biohythane composition. Additionally, the system demonstrated efficient removal of chemical oxygen demand and ammonium, suggesting potential field applications for the single-stage biohythane fermentation technology.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Environmental Sciences
Anish Ghimire, Vincenzo Luongo, Luigi Frunzo, Piet N. L. Lens, Francesco Pirozzi, Giovanni Esposito
Summary: The study found that methane production was higher under high temperature anaerobic digestion tests compared to mesophilic tests, while hydrogen yield was higher in mesophilic dark fermentation tests.
ENVIRONMENTAL TECHNOLOGY
(2022)
Article
Oncology
Tyler Shugg, Reynold C. Ly, Wilberforce Osei, Elizabeth J. Rowe, Caitlin A. Granfield, Ty C. Lynnes, Elizabeth B. Medeiros, Jennelle C. Hodge, Amy M. Breman, Bryan P. Schneider, S. Cenk Sahinalp, Ibrahim Numanagic, Benjamin A. Salisbury, Steven M. Bray, Ryan Ratcliff, Todd C. Skaar
Summary: This study assessed the accuracy of the Aldy computational method in extracting pharmacogenomics genotypes from whole genome sequencing (WGS) and whole exome sequencing (WES) data. The results showed that Aldy achieved over 99% accuracy in calling diplotypes for major pharmacogenes from both WGS and WES data, supporting its importance in clinical applications.
FRONTIERS IN ONCOLOGY
(2023)
Article
Microbiology
Ilayda Akacin, Seymanur Ersoy, Osman Doluca, Mine Gungormusler
Summary: This review provides a comprehensive overview of recent literature on the utilization of TGS and NGS technologies in microbial metagenomics research. It discusses the advantages and limitations of these technologies and presents real-time examples of novel applications in clinical microbiology and public health, food and agriculture, energy and environment, arts and space.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Virology
Zilei Zhang, Danlei Liu, Dapeng Wang, Qingping Wu
Summary: The study introduced a new method called TRACE-seq for preparing next generation sequencing libraries for Human noroviruses (HuNoVs), allowing almost complete viral genome attainment and demonstrating high reliability and versatility.
Article
Ecology
Zi-Wen Yang, Yu Men, Jing Zhang, Zhi-Hui Liu, Jiu-Yang Luo, Yan-Hui Wang, Wen-Jun Li, Qiang Xie
Summary: The study found that air dried preservation method had poor effects on insect microbiome samples, while RNAlater was not suitable for longer field works. CTAB and ethanol were recommended as more effective and cost-efficient preservatives. Additionally, NGS sequencing of the V4 region of the 16S rRNA gene had lower resolution compared to TGS sequencing, potentially leading to misestimations of microbial community compositions.
Article
Biochemistry & Molecular Biology
Markus Pfenninger, Philipp Schoennenbeck, Tilman Schell
Summary: Accurate estimation of genome sizes is essential in biodiversity genomics, and this study introduces a method that can estimate genome size from the number of sequenced bases and mean sequencing depth. Simulations demonstrate that even from low-coverage genome drafts, reasonable estimates can be obtained using this method. Comparison with flow cytometry estimates suggests that both methods provide similar and interchangeable results.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Biochemical Research Methods
Timo Lassmann
Summary: SAMStat is an efficient program for extracting quality control metrics from fastq and SAM/BAM files. It displays sequence composition, base quality composition, and mapping error profiles based on mapping quality, allowing users to quickly identify reasons for poor mapping. A major update to SAMStat now supports paired-end and long-read data, with quality control plots drawn using the ploty javascript library.
Article
Engineering, Environmental
Samson Oluwasegun Masebinu, Olufunto Tolulope Fanoro, Heribert Insam, Charles Mbohwa, Andreas Otto Wagner, Rudolf Markt, Sebastian Hupfauf
Summary: This study evaluated the impact of biochar addition to AD reactors on productivity, efficiency, and stability. The results showed that biochar effectively reduced H2S production but had no effect on biogas and methane yields. It also did not alter the microbial community composition.
ENVIRONMENTAL ENGINEERING RESEARCH
(2022)
Article
Microbiology
Srisuda Chaikitkaew, Supattra In-Chan, Apinya Singkhala, Wisarut Tukanghan, Chonticha Mamimin, Alissara Reungsang, Nils-Kare Birkeland, Sompong O-Thong
Summary: A new strain PL3(T) of the genus Clostridium, capable of producing acetate, was isolated and characterized in this study. The strain exhibited wide growth conditions and diverse metabolic capabilities. Phylogenetic analysis and DNA-DNA hybridization confirmed that PL3(T) represents a novel species in the genus Clostridium.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Julia Vinzelj, Akshay Joshi, Diana Young, Ljubica Begovic, Nico Peer, Lona Mosberger, Katharina Cecile Schmid Luedi, Heribert Insam, Veronika Flad, Magdalena Nagler, Sabine Marie Podmirseg
Summary: Anaerobic fungi have the ability to degrade lignocellulosic biomass, and their preservation methods have been explored in this study. Cryopreservation in liquid nitrogen and agar preservation at 39 degrees C were found to be effective methods for long-term storage of these fungi.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Akshay Joshi, Diana Young, Liren Huang, Lona Mosberger, Bernhard Munk, Julia Vinzelj, Veronika Flad, Alexander Sczyrba, Gareth W. Griffith, Sabine Marie Podmirseg, Rolf Warthmann, Michael Lebuhn, Heribert Insam
Summary: This study reveals the selective enrichment of certain genera in media with rumen fluid and depleted rumen fluid from rumen and non-rumen samples,respectively. Additionally, five new clades were defined from fecal samples of llama, guanaco, yak, and elephant. Therefore, the non-rumen fluid medium is recommended for isolating anaerobic fungi from non-rumen environments.
Article
Environmental Sciences
Maria Gomez-Brandon, Marta Lores, Jorge Dominguez
Summary: The study evaluated the effectiveness of vermicomposting for the valorization of distilled grape marc. It found that the marc was suitable as feedstock to sustain large earthworm populations, with pH and electrical conductivity reaching optimum levels. After 14 days of vermicomposting, there was a rapid decrease in microbial activity and total polyphenol content, indicating stabilization. The end product had the necessary macro- and micronutrients for good vermicompost quality, suitable for plant health and safe agricultural use.
JOURNAL OF MATERIAL CYCLES AND WASTE MANAGEMENT
(2023)
Article
Biochemistry & Molecular Biology
Kamolwan Thepsuthammarat, Alissara Reungsang, Pensri Plangklang
Summary: Improving biomass production by using low-cost substrates is important for large-scale microalgae production. In this study, the microalga Coelastrella sp. KKU-P1 was cultivated mixotrophically using unhydrolyzed molasses as a carbon source. The optimal conditions for maximizing biomass production were determined, and a batch cultivation achieved the highest biomass production of 3.81 g/L. CO2 supplementation did not improve biomass production, indicating that ambient CO2 concentration was sufficient for mixotrophic growth. The biochemical analysis showed that the microalgal biomass has potential as a source of essential amino acids, pigments, and fatty acids.
Editorial Material
Microbiology
Michael Lebuhn, Sabine Marie Podmirseg, Urs Baier
Article
Plant Sciences
Elena Tello-Garcia, Edoardo Mandolini, Erich Tasser, Maraike Probst, Ursula Peintner, Ulrike Tappeiner, Georg Leitinger
Summary: The effects of plant-soil interactions on plant productivity and traits under drought conditions were investigated in this study. The reduction of soil microbial load by soil steam sterilization resulted in increased plant productivity under wet conditions and led to plant traits associated with fast-growing strategies. Drought in sterilized soil decreased productivity, promoted earlier wilting and resulted in the development of plant traits associated with a more slow-growing strategy.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Environmental Sciences
Marisa Raketh, Rusnee Kana, Prawit Kongjan, Syed Anuar Faua'ad Muhammad, Sompong O-Thong, Chonticha Mamimin, Rattana Jariyaboon
Summary: This study investigates the biohydrogen and biomethane potential of co-digestion from palm oil mill effluent (POME) and concentrated latex wastewater (CLW). It finds that specific mixing ratios can achieve the highest yields of hydrogen and methane, and continuous reactors also produce high amounts of biogas.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Multidisciplinary Sciences
Casey H. H. Meili, Adrienne L. L. Jones, Alex X. X. Arreola, Jeffrey Habel, Carrie J. J. Pratt, Radwa A. A. Hanafy, Yan Wang, Aymen S. S. Yassin, Moustafa A. A. TagElDein, Christina D. D. Moon, Peter H. H. Janssen, Mitesh Shrestha, Prajwal Rajbhandari, Magdalena Nagler, Julia M. M. Vinzelj, Sabine M. M. Podmirseg, Jason E. E. Stajich, Arthur L. L. Goetsch, Jerry Hayes, Diana Young, Katerina Fliegerova, Diego Javier Grilli, Roman Vodicka, Giuseppe Moniello, Silvana Mattiello, Mona T. T. Kashef, Yosra I. I. Nagy, Joan A. A. Edwards, Sumit Singh Dagar, Andrew P. P. Foote, Noha H. H. Youssef, Mostafa S. S. Elshahed
Summary: Through analyzing 661 fecal samples, it was found that anaerobic gut fungal (AGF) diversity is much more abundant than previously estimated, with the identification of 56 novel genera. The diversity of AGF is influenced by the host's phylogenetic affiliation, and the fungal-host associations are stronger and more specific in hindgut fermenters.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Rattana Jariyaboon, Surananee Hayeeyunu, Nikannapas Usmanbaha, Shahrul Bin Ismail, Sompong O-Thong, Chonticha Mamimin, Prawit Kongjan
Summary: Food waste, a type of organic solid waste, has a negative impact on environmental sustainability. Dark fermentation was used to simultaneously generate mixed volatile fatty acids (VFAs) and bio-hydrogen from food waste. The study found that the pretreated inoculum was capable of converting 10 g VS/L food waste to hydrogen with a high yield of 135.2 ± 7 mL H-2/VSadded.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Gede Adi Wiguna Sudiartha, Tsuyoshi Imai, Chonticha Mamimin, Alissara Reungsang
Summary: Temperature plays a significant role in anaerobic digestion, affecting microbial communities and process efficiency. This study tested two temperature shift scenarios and found that both led to a decrease in biogas production, with the upshifted scenario experiencing a larger decrease. 16S rRNA sequencing revealed that the dominant methanogens differed between the two scenarios, indicating that temperature shifts affect microbial communities and biogas production.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Sureewan Sittijunda, Sulfan Baka, Rattana Jariyaboon, Alissara Reungsang, Tsuyoshi Imai, Prawit Kongjan
Summary: This study aimed to enhance dark fermentative hydrogen production from co-digestion of distillery wastewater and glycerol waste. The optimal ratio of DW and GW for hydrogen production was found to be 99:1, resulting in a hydrogen yield of 149.5 mL-H-2/g - VSadded. The dark fermentation effluent was then used for methane production, with a maximum methane yield of 115.1 mL-CH4/g - VSadded. Microbial community analysis revealed the presence of methanogenic archaea in the microbial electrolysis cell reactor.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Marika Ngamsirisomsakul, Mallika Boonmee Kongkeitkajorn, Sittipong Amnuaypanich, Alissara Reungsang
Summary: Sugarcane bagasse can be used as a raw material for microbial lipid production, and incorporating glycerol into unconcentrated bagasse hydrolysate helps improve growth and lipid production in Rhodotorula glutinis TISTR 5159. When mixed with 10% v/v glycerol, the hydrolysate with yeast extract as the sole nitrogen source achieved the highest lipid yield.
FERMENTATION-BASEL
(2022)
Article
Environmental Studies
Tommaso Bardelli, Shamina Imran Pathan, Paola Arfaioli, Nadia Vignozzi, Sergio Pellegrini, Flavio Fornasier, Markus Egli, Maria Gomez-Brandon, Heribert Insam, Giacomo Pietramellara, Judith Ascher-Jenull
Summary: This study evaluated the effects of slope exposure on the physico-chemical and microbiological properties of soil in an Italian alpine forest. The results showed that slope exposure significantly influenced soil properties and enzyme activities. The effects varied between north-facing and south-facing slopes, as well as among soil horizons and aggregate size fractions.