Journal
IMMUNOLOGY AND CELL BIOLOGY
Volume 97, Issue 6, Pages 552-562Publisher
WILEY
DOI: 10.1111/imcb.12244
Keywords
Adaptive immunity; dendritic cells; MHC-I; NLRC5; rotavirus; small intestine
Categories
Funding
- Canada's National Microbiology Laboratory
- Canadian Institutes of Health Research [THC-13523]
- National Health and Medical Research Council (NHMRC) of Australia [APP1107930]
- Victorian State Government Operational Infrastructure Scheme
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The NOD-like receptor (NLR) family plays an important role in innate immunity. Class II transactivator and NOD-like receptor caspase activation and recruitment domain CARD containing 5 (NLRC5) are unusual members of the NLR family that instead of recognizing pathogen-associated or damage-associated molecular patterns, form enhanceosomes with adaptor molecules and modulate major histocompatibility complex (MHC) class II and MHC class I expression, respectively. While NLRC5 has been shown to play a role during intracellular pathogen infection and tumor cell immune evasion, its role in regulating antigen-specific CD8(+) T-cell responses at the intestinal mucosa has not been investigated. Here, we take advantage of the rotavirus model in adult mice to dissect the impact of NLRC5 on CD8(+) T-cell responses to this viral infection at the gut mucosa. We show that while Nlrc5(-/-) mice exhibited normal proportions of T-cell subpopulations in the intraepithelial and lamina propria compartments, these mice had decreased baseline MHC class I expression on various immune cells in the lamina propria. Upon rotavirus infection, Nlrc5 deficiency resulted in impaired H2-K-b-restricted antigen-specific CD8(+) T-cell responses, which were recapitulated in mice deficient for Nlrc5 within the dendritic cell compartment. The impaired CD8(+) T-cell response in Nlrc5(-/-) mice was not significant enough to impact viral titers, suggesting compensation in Nlrc5(-/-) mice, perhaps as a result of higher numbers of activated B cells in the mesenteric lymph nodes and normal rotavirus-specific immunoglobulin A responses. Collectively, our results demonstrate a minor role for NLRC5 in modulating H2-K-b-restricted antigen-specific CD8(+) T-cell responses in the small intestine during rotavirus infection in adult mice.
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