Article
Immunology
Sirikorn Kitiyodom, Teerapong Yata, Kim D. Thompson, Janina Costa, Preetham Elumalai, Takayuki Katagiri, Sasithon Temisak, Katawut Namdee, Channarong Rodkhum, Nopadon Pirarat
Summary: The study confirmed that the chitosan-based mucoadhesive nanovaccine is an effective platform for immersion vaccination of tilapia, with vaccinated fish generating a humoral systemic immune response against columnaris disease. Fish mortality rate significantly decreased in the vaccinated group compared to the unvaccinated control group.
Article
Fisheries
Sirikorn Kitiyodom, Clara Trullas, Channarong Rodkhum, Kim D. Thompson, Takayuki Katagiri, Sasithon Temisak, Katawut Namdee, Teerapong Yata, Nopadon Pirarat
Summary: The study focused on developing a mucoadhesive nano-immersion vaccine against columnaris disease in tilapia, using field isolates of Flavobacterium columnare obtained from clinical outbreaks in red tilapia. Results showed that the nano-vaccine induced greater antigen uptake and immune response in fish than conventional whole cell killed vaccines.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Mamdouh Y. Elgendy, Mohamed Abdelsalam, Samaa Ahmed Mohamed, Shimaa E. Ali
Summary: Columnaris is a common flavobacterial disease that affects tilapia aquaculture, causing heavy mortalities. The study analyzed the characteristics of the pathogen and identified key factors that may help control the disease.
AQUACULTURE INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Rachel A. Conrad, Jason P. Evenhuis, Ryan S. Lipscomb, Clayton Birkett, Mark J. McBride
Summary: Columnaris disease caused by Flavobacterium columnare is a major challenge for freshwater aquaculture. The virulence factors and control methods of F. columnare are poorly understood. This study revealed that iron acquisition mechanisms, such as siderophores, are not critical virulence factors for F. columnare.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Fisheries
Anurak Bunnoy, Kim D. Thompson, Patcharapong Thangsunan, Putita Chokmangmeepisarn, Teerapong Yata, Nopadon Pirarat, Sirikorn Kitiyodom, Pattanapong Thangsunan, Pimwarang Sukkarun, Pochara Prukbenjakul, Naphat Panthukumphol, Manami Morishita, Prapansak Srisapoome, Channarong Rodkhum
Summary: A new bivalent mucoadhesive nanovaccine was developed for immersion vaccination of tilapia against francisellosis and columnaris disease. It has the potential to improve innate and adaptive immunity in vaccinated fish, increasing survival rates.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Prapansak Srisapoome, Kubpaphas Thummabancha, Ratree Wongpanya
Summary: This study successfully cloned and characterized two cDNAs encoding Nile tilapia DnaJ proteins, revealing their gene structures and tissue expression patterns under normal and pathogenic infection conditions. Silencing of these genes confirmed their crucial roles in response to heat and bacterial stress, suggesting their involvement in regulating intracellular proteins during infection.
Article
Fisheries
Ramasamy Harikrishnan, Gunapathy Devi, Hien Van Doan, Chellam Balasundaram, Jesu Arockiaraj, Chandrasekar Jagruthi
Summary: The study demonstrates that feeding a diet enriched with ulvan can significantly improve growth, antioxidant system, immune defense system, and immunoantioxidant related gene expression in Labeo rohita against Flavobacterium columnaris.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Zijun Lu, Ren Gao, Yafei Duan, Rui Han, Wenjie Guo, Xueming Dan, Yanwei Li
Summary: This study isolated 45 bacterial strains of F. columnare from grass carp in various provinces of China, identifying three genotypes with genomovar I being the dominant type. Analysis revealed genetic differences among the three genomovars, with a closer relationship between genomovar I and II. The study provides insight into the genetic diversity of F. columnare in Chinese grass carp.
Article
Immunology
Wenlong Cai, Covadonga R. Arias
Summary: A modified-live vaccine against columnaris disease in catfish aquaculture was developed by selecting mutants resistant to rifampin. Comparative genomic analysis revealed 16 unique single nucleotide polymorphisms in the attenuated mutant strain, affecting RNA polymerase activity and potentially disrupting protein secretion systems.
Article
Fisheries
Shivam Singh, Sumanta Kumar Mallik, Krishna Kala, Neetu Shahi, Richa Pathak, Abhay Kumar Giri, Suresh Chandra, Kushagra Pant, Rabindar Singh Patiyal
Summary: The study identified Flavobacterium columnare as the causative agent in columnaris disease in farmed rainbow trout in Central Indian Himalayan region. It confirmed the high virulence of the F. columnare BRTAGILLFC 01 strain through in-vitro assays and experimental infection in rainbow trout fingerlings. The study also revealed the susceptibility of the bacterium to certain antibiotics at lower concentrations, demonstrating the potential for antibiotic treatment in disease control.
Article
Fisheries
Weiwei Zeng, Yingying Wang, Xiaoyu Chen, Qing Wang, Sven M. Bergmann, Ying Yang, Yahui Wang, Bo Li, Yuefeng Lv, Hua Li, Wensheng Lan
Summary: The VP20-based vaccine can elicit both humoral and cellular immune responses and significantly protect fish against infectious challenge by Tilapia lake virus (TiLV). The DNA prime-protein boost immunization strategy showed the potential advantage over solo vaccination.
Article
Fisheries
Marwa El-Naggar, Sally Salaah, Hamza El-Shabaka, Fawzia Abd El-Rahman, Magdy Khalil, Ashraf Suloma
Summary: Chitosan is environmentally friendly and can improve the health status and disease resistance of Nile tilapia when added as a dietary supplement, especially in plant-based diets.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Nantawut Ponpukdee, Pradit Wangman, Channarong Rodkhum, Chalinan Pengsuk, Parin Chaivisuthangkura, Paisarn Sithigorngul, Siwaporn Longyant
Summary: Monoclonal antibodies generated from outer membrane proteins of pathogenic Flavobacterium columnare can be used as a simple, fast, specific, and low-cost immunological tool for the direct detection of F. columnare without the need for bacterial isolation as in conventional biochemical tests or DNA extraction as in PCR assays. By combining specific MAbs, the detection limit in dot blotting assay can be increased to 5 x 10(2) cfu/dot.
Article
Fisheries
Anurak Bunnoy, Patcharapong Thangsunan, Putita Chokmangmeepisarn, Teerapong Yata, Nawanith Klongklaew, Nopadon Pirarat, Sirikorn Kitiyodom, Prapansak Srisapoome, Channarong Rodkhum
Summary: This study developed a novel nano-encapsulated vaccine for Columnaris disease, which showed significant immunological and protective effects. It has the potential to be used on a large scale in the freshwater aquaculture industry.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nicole C. Thunes, Rachel A. Conrad, Haitham H. Mohammed, Yongtao Zhu, Paul Barbier, Jason P. Evenhuis, David Perez-Pascual, Jean-Marc Ghigo, Ryan S. Lipscomb, John R. Schneider, Nan Li, Devon H. Erbes, Clayton Birkett, Benjamin R. LaFrentz, Timothy J. Welch, Mark J. McBride
Summary: Flavobacterium columnare, a pathogen that causes columnaris disease in freshwater fish, poses a major threat to sustainable aquaculture. Researchers have identified a genetically manipulable strain, MS-FC-4, which has facilitated the identification of virulence factors. Deletion of core genes eliminates virulence, and several secreted proteins have been identified as important for pathogenicity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Fisheries
Mahmoud Mabrok, Sivaramasamy Elayaraja, Putita Chokmangmeepisarn, Wansadaj Jaroenram, Narong Arunrut, Wansika Kiatpathomchai, Partho Pratim Debnath, Jerome Delamare-Deboutteville, Chadag Vishnumurthy Mohan, Aml Fawzy, Channarong Rodkhum
Summary: A new method for detecting F. columnare, using a combination of RPA and LFD technologies, has been developed with high efficiency, speed, and accuracy. The method shows no cross-reactivity with other bacteria and is suitable for field application and low-resource settings.
Article
Fisheries
Partho Pratim Debnath, Nguyen Dinh-Hung, Suwimon Taengphu, Vuong Viet Nguyen, Jerome Delamare-Deboutteville, Saengchan Senapin, Chadag Vishnumurthy Mohan, Ha Thanh Dong, Channarong Rodkhum
Summary: Sixteen countries have reported the presence of tilapia lake virus (TiLV), an emerging pathogen affecting tilapia. In Bangladesh, 20% of farms tested positive for TiLV in tilapia, while co-cultivated fish species and invertebrates did not show signs of TiLV infection. Experimental infection only showed clinical signs in Nile tilapia, suggesting that species in polycultures may not be susceptible to TiLV.
JOURNAL OF FISH DISEASES
(2022)
Article
Immunology
Sirikorn Kitiyodom, Teerapong Yata, Kim D. Thompson, Janina Costa, Preetham Elumalai, Takayuki Katagiri, Sasithon Temisak, Katawut Namdee, Channarong Rodkhum, Nopadon Pirarat
Summary: The study confirmed that the chitosan-based mucoadhesive nanovaccine is an effective platform for immersion vaccination of tilapia, with vaccinated fish generating a humoral systemic immune response against columnaris disease. Fish mortality rate significantly decreased in the vaccinated group compared to the unvaccinated control group.
Article
Immunology
Thao Thu Mai, Pattanapon Kayansamruaj, Chayanit Soontara, Pattarawit Kerddee, Dinh-Hung Nguyen, Saengchan Senapin, Janina Z. Costa, Jorge del-Pozo, Kim D. Thompson, Channarong Rodkhum, Ha Thanh Dong
Summary: Immunization of tilapia broodstock with TiLV vaccines can induce antibody response and transfer maternal antibodies to fertilized eggs and larvae, providing protection against TiLV. HKV appears to be more promising than FKV for maternal vaccination.
Article
Multidisciplinary Sciences
Ranida Tuanudom, Nichapat Yurayart, Channarong Rodkhum, Sonthaya Tiawsirisup
Summary: A study on the diversity of midgut bacteria in laboratory-colonized and field-collected Ae. albopictus revealed differences in bacterial composition, which may be influenced by factors like blood meal source, diet, or habitat. The findings emphasize the importance of understanding the role of midgut bacteria in shaping microbial communities in Ae. albopictus for effective mosquito and disease control programs.
Article
Fisheries
Muhammad Fadhlullah Mursalim, Hendri Budiyansah, Hartanto Mulyo Raharjo, Partho Pratim Debnath, Rungnapa Sakulworakan, Putita Chokmangmeepisarn, Jitrapa Yindee, Patharapol Piasomboon, Sivaramasamy Elayaraja, Channarong Rodkhum
Summary: This study investigated the distribution, diversity, and antimicrobial susceptibility patterns of Aeromonas spp. in freshwater fishes in Thailand. The results revealed that A. veronii is the most abundant species in Thai cultured freshwater fish with the highest multidrug resistance patterns.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Patcharapong Thangsunan, Sirikorn Kitiyodom, Prapansak Srisapoome, Nopadon Pirarat, Teerapong Yata, Pattanapong Thangsunan, Suwimon Boonrungsiman, Anurak Bunnoy, Channarong Rodkhum
Summary: A mucoadhesive nanovaccine platform was developed using cetyltrimethylammonium bromide (CTAB) to improve the efficiency of immersion vaccination against Francisella noatunensis subsp. orientalis (Fno) in tilapia. The CAT-Fno-NV increased the opportunity of Fno vaccine uptake and enhanced the protective efficacy against Fno infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Anurak Bunnoy, Patcharapong Thangsunan, Putita Chokmangmeepisarn, Teerapong Yata, Nawanith Klongklaew, Nopadon Pirarat, Sirikorn Kitiyodom, Prapansak Srisapoome, Channarong Rodkhum
Summary: This study developed a novel nano-encapsulated vaccine for Columnaris disease, which showed significant immunological and protective effects. It has the potential to be used on a large scale in the freshwater aquaculture industry.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Clara Trullas, Mariya Sewaka, Channarong Rodkhum, Nantarika Chansue, Surintorn Boonanuntanasarn, Manoj Tukaram Kamble, Nopadon Pirarat
Summary: This study aimed to evaluate the effects of a Jerusalem artichoke-supplemented diet on the blood chemistry, growth performance, intestinal morphology, expression of antioxidant-related genes, and disease resistance against Aeromonas veronii challenge in juvenile red tilapia. The results showed that the JA-supplemented diets had beneficial effects on the growth performance, blood chemistry, and intestinal morphology. Moreover, the prebiotic diets increased the expression of antioxidant-related genes and enhanced disease resistance against A. veronii in red tilapia. Therefore, Jerusalem artichoke could be used as a promising prebiotic for sustainable red tilapia farming.
Article
Agriculture, Dairy & Animal Science
Krissda Boonaramrueng, Navapon Techakriengkrai, Channarong Rodkhum, Rosama Pusoonthornthum
Summary: This study investigated the associated risks of Bartonella spp. infection in retroviral-infected cats and its impact on T-lymphocyte subsets. The results showed that the prevalence of Bartonella spp. infection was 16.1% and the seroprevalence was 94.9%. Cats with concurrent retrovirus and Bartonella spp. infection had a significant reduction in the CD4(+)/CD8(+) ratio. Therefore, cat owners in endemic areas should be aware of the risk of Bartonella spp. exposure, and the CD4(+)/CD8(+) ratio may serve as a novel marker for bartonellosis.
Article
Agriculture, Dairy & Animal Science
Jakarwan Yostawonkul, Manoj Tukaram Kamble, Kitikarn Sakuna, Sulaiman Madyod, Pimwarang Sukkarun, Seema Vijay Medhe, Channarong Rodkhum, Nopadon Pirarat, Mariya Sewaka
Summary: Natural infections caused by Aeromonas veronii in intensive tilapia farming can lead to economic losses. The use of mangosteen peel extract loaded in nanoemulsion (MSNE)-supplemented diets in Nile tilapia can improve growth performance, immune response, and disease resistance. The particle size, polydispersity index, and particle surface charge of MSNE were 151.9 +/- 1.4 nm, >0.3, and -30 mV, respectively. MSNE improved the in vitro inhibition against A. veronii and had beneficial effects on growth performance, immune response, and disease resistance in Nile tilapia.
Article
Fisheries
Geraldine B. Dayrit, Emmanuel Vera M. Cruz, Channarong Rodkhum, Mahmoud Mabrok, Pattareeya Ponza, Mudjekeewis D. Santos
Summary: This study investigated the potential effects of shading on water quality parameters, blood indices, and serum biochemical profiles of cultured Nile tilapia. The results showed that shading can reduce water temperature, alleviate stress in fish, and lessen the impact of temperature warming on tilapia production.
Article
Fisheries
Dharacha Suwanbumrung, Sudthirak Wongkhieo, Wisawat Keaswejjareansuk, Piroonrat Dechbumroong, Manoj Tukaram Kamble, Teerapong Yata, Sirikorn Kitiyodom, Channarong Rodkhum, Kim D. Thompson, Katawut Namdee, Nopadon Pirarat
Summary: This study aimed to develop a novel cationic-based nanoemulsion vaccine containing bile salts (NEB) coated by chitosan (CS) for oral vaccination of Nile tilapia against S. agalactiae. The NEB-CS vaccine exhibited improved mucoadhesiveness, permeability, and protective efficacy compared to the control group. The results suggest that NEB-CS can be a promising approach to protect tilapia in aquaculture against streptococcosis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Immunology
Kim D. Thompson, Channarong Rodkhum, Anurak Bunnoy, Patcharapong Thangsunan, Sirikorn Kitiyodom, Pimwarang Sukkarun, Jakarwan Yostawornkul, Teerapong Yata, Nopadon Pirarat
Summary: Tilapia is extensively farmed and provides an important source of food and income for low-income families. However, disease outbreaks caused by bacteria and viruses have increased with the intensification of farming systems. Nanoparticle-based vaccines offer potential solutions to improve vaccine acceptance and uptake among tilapia farmers.
Article
Veterinary Sciences
Rajit Lohajaroensub, Chenphop Sawangmake, Channarong Rodkhum, Nalinee Tuntivanich
Summary: This study aimed to determine the expression of peptide genes of natural antimicrobials in canine amniotic membrane. The results showed that canine beta-defensin 1, 103, and Elafin were expressed in all samples, possibly suggesting a role in the innate immune system of normal canine amniotic membrane.
VETERINARY SCIENCES
(2022)