Review
Cell Biology
Meng Feng, Shuping Zhou, Yong Yu, Qinghong Su, Xiaofan Li, Wei Lin
Summary: Dendritic cells (DCs) are a class of antigen-presenting cells with crucial roles in immune activation and tolerance. Understanding the migration mechanisms and regulatory factors of different DC subgroups provides new insights for disease treatment, such as infection, tumors, and vaccine preparation. Various factors, including chemokines, membrane molecules, and microenvironments, influence DC migration and immunological functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhigang Chen, Rong Fan, Jie Liang, Zexiu Xiao, Junlong Dang, Jun Zhao, Ruihui Weng, Cansheng Zhu, Song Guo Zheng, Ying Jiang
Summary: This study found that NFIL3 deficiency could alleviate MOG35-55-induced EAE by regulating different immune cell subsets, especially the crosstalk between CD4+ T cells and CD11c+ dendritic cells.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Geriatrics & Gerontology
Yan Li, Pei Chen, Hao Huang, Huiyu Feng, Hao Ran, Weibin Liu
Summary: This study found that all common DC subsets are present in the human thymus of various ages, with the density of pDCs gradually increasing with age, suggesting an additional role of pDCs in the thymus beyond central tolerance. The ratio of medulla to cortex area in the human thymus increases with age, while the densities of different DC subsets also show changes.
Article
Biology
Muhammad Riadul Haque Hossainey, Kelsey A. Hauser, Christina N. Garvey, Namarta Kalia, Juliette M. Garvey, Leon Grayfer
Summary: The study found that two different types of amphibian macrophage (M phi) cells have similar transcriptomes and functions, resembling FLT3L-derived frog dendritic cells (DCs) and CSF1-M phi, respectively. These findings provide unique insights into the evolutionarily retained and diverged pathways of M phi and DC functional differentiation.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Johannes Nordlohne, Ilona Hulsmann, Svenja Schwafertz, Jasmin Zgrajek, Manuel Grundmann, Sibylle von Vietinghoff, Frank Eitner, Michael S. Becker
Summary: The study identified five distinct renal MNP subsets and found that MNP composition and functional segmentation varied between different mouse models of kidney injury, with dynamic regulation over time. CD206 + cells from three analyzed MNP subsets exhibited higher ex vivo phagocytic capacity than CD86 + MHCII + CD206- counterparts, highlighting the functional uniqueness of each subset.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Amairelys Belen Barroeta Seijas, Sonia Simonetti, Irene Filippi, Antonella Naldini, Gabriele Favaretto, Teresa Colombo, Ambra Natalini, Fabrizio Antonangeli, Mattia Laffranchi, Silvano Sozzani, Angela Santoni, Francesca Di Rosa
Summary: Dendritic cells (DCs) play a crucial role in immunity and tolerance, and studies have found that DCs exhibit a hypoxic phenotype in the spleen and bone marrow. DCs expressing membrane c-kit, the receptor for stem cell factor, showed higher hypoxic marker intensity under low oxygen conditions.
CELL BIOCHEMISTRY AND FUNCTION
(2022)
Review
Immunology
Ioana Plesca, Luise Mueller, Jan P. Boettcher, Hind Medyouf, Rebekka Wehner, Marc Schmitz
Summary: Dendritic cells (DCs) are crucial in orchestrating anti-tumor immunity, capable of producing proinflammatory cytokines, initiating T-cell responses, and exhibiting direct cytotoxicity against tumor cells. The functionality of DCs depends on intrinsic properties and the tumor microenvironment, with immunogenic DCs promoting tumor elimination and tolerogenic DCs fostering tumor progression.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Benjamin Hamid, Friederike Ebner, Lalita Bechtold, Arkadi Kundik, Sebastian Rausch, Susanne Hartmann
Summary: The study found that the relative frequencies of DC subsets in porcine blood differ, with A. suum E/S showing differential modulation on different DC subsets, suppressing pDC and cDC1 while upregulating cDC2.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Cameron W. Paterson, Melissa B. Gutierrez, Craig M. Coopersmith, Mandy L. Ford
Summary: Chronic alcohol use leads to T cell dysregulation within the adaptive immune system. In this study, using a murine model, researchers compared rare splenic subpopulations in alcohol-exposed mice and water-fed controls. The results showed decreased naive T cell populations and alterations in effector regulatory T cell phenotypes in chronic alcohol-induced immune dysfunction.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Lei Zhang, Wei Zhao, Jinke Huang, Fangxuan Li, Jindong Sheng, Hualin Song, Ying Chen
Summary: A new tumor vaccine, FCM-NPs, was developed by fusing DCs and OC cells and coating them onto nanoparticles, showing promising results in promoting immune responses and inhibiting tumor metastasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Maximilian Bruckner, Michael Fichter, Richard da Costa Marques, Katharina Landfester, Volker Mailaender
Summary: Successful cell targeting depends on the controlled positioning of cell-type-specific antibodies on the nanocarrier's surface. The length of PEG linker significantly affects the targeting efficiency of dendritic cells, and the use of appropriate PEG length is crucial for specific accumulation in different dendritic cell subsets.
Article
Dentistry, Oral Surgery & Medicine
H. Sharawi, O. Heyman, G. Mizraji, Y. Horev, A. Laviv, L. Shapira, S. Yona, A. H. Hovav, A. Wilensky
Summary: Dendritic cells (DCs) play a crucial role in immunity and tolerance induction, including in oral mucosa homeostasis. This study identified four subsets of DCs in healthy gingiva and found dysregulation of gingival DC subsets in periodontal patients, with an impact on cytokine expression. Smoking was also shown to reduce gingival LCs in healthy individuals, potentially contributing to the severity of periodontitis in smokers. These findings lay the groundwork for understanding the role of human DCs in oral immunity and the modulation of immunity in periodontitis.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Xiaojing Xu, Minfeng Gan, Youzhen Ge, Cheng Yi, Tianyun Feng, Mengjie Liu, Cenhao Wu, Xiang Chen, Weidong Zhang, Lixiang Zhao, Jun Zou
Summary: Chemical conjugation of CpG DNA to glucose-containing glycopolymers can enhance the therapeutic effects of CpG-based vaccines against tumors. The vaccine was found to inhibit both primary and metastatic melanoma in mice, dependent on CD8 + T cells and IFN gamma. Furthermore, in the tumor microenvironment, treatment with gCpG led to increased Th1 and CTL infiltration, increased M1 macrophages, decreased Tregs and MDSCs populations, and promoted an inflammatory milieu with enhanced secretion of IFN gamma and TNF alpha. The anti-tumor efficacy of gCpG was significantly enhanced when combined with anti-PD1 immunotherapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Immunology
Kaela Drzewiecki, Jungmin Choi, Joseph Brancale, Michael A. Leney-Greene, Sinan Sari, Buket Dalgic, Aysel Unlusoy Aksu, Gulseren Lvigen Sahin, Ahmet Ozen, Safa Baris, Elif Karakoc-Aydiner, Dhanpat Jain, David Kleiner, Michael Schmalz, Kadakkal Radhakrishnan, Junhui Zhang, Kasper Hoebe, Helen C. Su, Joao P. Pereira, Michael J. Lenardo, Richard P. Lifton, Silvia Vilarinho
Summary: Portal hypertension is a major contributor to decompensation and death from liver disease. Research shows that homozygous damaging mutations in GIMAP5 can lead to capillarization of liver endothelial cells and ultimately result in portal hypertension. Understanding the pathogenesis of advanced liver disease is crucial for developing new therapies to reduce adverse outcomes.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Review
Immunology
Elodie Segura
Summary: This review provides an updated view on human DC subsets and their ontogeny, supported by recent clinical studies. The review also summarizes their main characteristics, including functional specialization.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Hematology
Francesco Saettini, Cecilia Poli, Jaime Vengoechea, Sonia Bonanomi, Julio C. Orellana, Grazia Fazio, Fred H. Rodriguez, Loreani P. Noguera, Claire Booth, Valentina Jarur-Chamy, Marissa Shams, Maria Iascone, Maja Vukic, Serena Gasperini, Manuel Quadri, Amairelys Barroeta Seijas, Elizabeth Rivers, Mario Mauri, Raffaele Badolato, Giovanni Cazzaniga, Cristina Bugarin, Giuseppe Gaipa, Wilma G. M. Kroes, Daniele Moratto, Monique M. van Ostaijen-ten Dam, Frank Baas, Silvere van der Maarel, Rocco Piazza, Zeynep H. Coban-Akdemir, James R. Lupski, Bo Yuan, Ivan K. Chinn, Lucia Daxinger, Andrea Biondi
Summary: Agammaglobulinemia, recurrent infections, hypertrophic cardiomyopathy and neutropenia were found in three novel patients in this study. Variants in the gene for folliculin interacting protein 1 (FNIP1) were identified, leading to impaired B-cell metabolism and development. This study highlights the importance of FNIP1 in B-cell development and metabolism.
Article
Multidisciplinary Sciences
Sonia Simonetti, Ambra Natalini, Giovanna Peruzzi, Alfredo Nicosia, Antonella Folgori, Stefania Capone, Angela Santoni, Francesca Di Rosa
Summary: The study describes a flow cytometric method for capturing antigen-specific T cells in different cell cycle phases by combining Ki67 and DNA staining with MHC-peptide-multimer staining. This method enables successful differentiation between CD8 T cells in G(0), G(1), and S-G(2)/M phases, providing potential for increased understanding of T cell response in vivo and improved immuno-monitoring analysis.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Biochemistry & Molecular Biology
Alessio Gerussi, Ambra Natalini, Fabrizio Antonangeli, Clara Mancuso, Elisa Agostinetto, Donatella Barisani, Francesca Di Rosa, Raul Andrade, Pietro Invernizzi
Summary: Drug-induced liver injury (DILI) is a challenging clinical event with various phenotypes and limited diagnostic and therapeutic tools. Recent advancements in genomics and immunology offer insights into the molecular aspects of DILI, helping to improve understanding and research in this field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Leticia Monin, Adam G. Laing, Miguel Munoz-Ruiz, Duncan R. McKenzie, Irene Del Molino Del Barrio, Thanussuyah Alaguthurai, Clara Domingo-Vila, Thomas S. Hayday, Carl Graham, Jeffrey Seow, Sultan Abdul-Jawad, Shraddha Kamdar, Elizabeth Harvey-Jones, Rosalind Graham, Jack Cooper, Muhammad Khan, Jennifer Vidler, Helen Kakkassery, Shubhankar Sinha, Richard Davis, Liane Dupont, Isaac Francos Quijorna, Charlotte O'Brien-Gore, Puay Ling Lee, Josephine Eum, Maria Conde Poole, Magdalene Joseph, Daniel Davies, Yin Wu, Angela Swampillai, Bernard North, Ana Montes, Mark Harries, Anne Rigg, James Spicer, Michael H. Malim, Paul Fields, Piers Patten, Francesca Di Rosa, Sophie Papa, Timothy Tree, Katie J. Doores, Adrian C. Hayday, Sheeba Irshad
Summary: The study aimed to evaluate the safety and immunogenicity of the BNT162b2 vaccine in cancer patients. The results showed poor efficacy of a single vaccine dose in cancer patients, with significantly increased immunogenicity in solid cancer patients after a vaccine boost at day 21. Therefore, it is recommended to prioritize cancer patients for an early second dose on day 21.
Review
Biochemistry & Molecular Biology
Eleonora Arico, Laura Bracci, Luciano Castiello, Francesca Urbani, Jean-Laurent Casanova, Filippo Belardelli
Summary: The outbreak of COVID-19 highlights the importance of the innate immune response and the role of type I interferons in antiviral defense. Deficient type I interferon activity contributes to disease progression, and early therapeutic use of IFN-beta and prophylactic campaigns with live attenuated vaccines may prevent future pandemics.
CYTOKINE & GROWTH FACTOR REVIEWS
(2022)
Review
Biochemical Research Methods
Irene del Molino del Barrio, Thomas S. Hayday, Adam G. Laing, Adrian C. Hayday, Francesca Di Rosa
Summary: This article discusses the use of high-content flow cytometric methodology to rapidly provide meaningful biological data in COVID-19 studies. High-throughput flow cytometry helps identify immune signatures and investigate the impact of SARS-CoV-2 exposure on cancer patients. Integration of flow cytometry data with other metrics can differentiate the immune profiles of COVID-19 patients from healthy donors and non-COVID respiratory infection patients.
Article
Health Care Sciences & Services
Liverana Lauretti, Tonia Cenci, Nicola Montano, Martina Offi, Martina Giordano, Valerio M. Caccavella, Antonella Mangraviti, Ludovico Agostini, Alessandro Olivi, Lucia Gabriele, Luigi M. Larocca, Roberto Pallini, Maurizio Martini, Quintino Giorgio D'Alessandris
Summary: The prognostic role of EGFRvIII in glioblastoma is controversial. However, this study found no association between EGFRvIII and worsened prognosis. Interestingly, female sex was found to be associated with better outcomes.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Oncology
Maria Laura De Angelis, Federica Francescangeli, Chiara Nicolazzo, Eljona Xhelili, Filippo La Torre, Lidia Colace, Alessandro Bruselles, Daniele Macchia, Sara Vitale, Paola Gazzaniga, Marta Baiocchi, Ann Zeuner
Summary: Metastasis is the leading cause of death in colorectal cancer patients, making it imperative to develop preclinical models that can replicate the process of metastasis at the individual patient level. In this study, an orthotopic patient-derived xenograft (PDX) model was established by directly implanting freshly dissociated colorectal cancer cells into the colon of immunocompromised mice. The findings suggest that ortho-PDX can be a useful preclinical model to study metastasis-related features and demonstrate that the epithelial-to-mesenchymal transition and stemness properties of primary colorectal tumors may play a crucial role in tumor engraftment.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Amairelys Belen Barroeta Seijas, Sonia Simonetti, Irene Filippi, Antonella Naldini, Gabriele Favaretto, Teresa Colombo, Ambra Natalini, Fabrizio Antonangeli, Mattia Laffranchi, Silvano Sozzani, Angela Santoni, Francesca Di Rosa
Summary: Dendritic cells (DCs) play a crucial role in immunity and tolerance, and studies have found that DCs exhibit a hypoxic phenotype in the spleen and bone marrow. DCs expressing membrane c-kit, the receptor for stem cell factor, showed higher hypoxic marker intensity under low oxygen conditions.
CELL BIOCHEMISTRY AND FUNCTION
(2022)
Article
Immunology
Martina Musella, Andrea Guarracino, Nicoletta Manduca, Claudia Galassi, Eliana Ruggiero, Alessia Potenza, Ester Maccafeo, Gwenola Manic, Luca Mattiello, Sara Soliman Abdel Rehim, Michele Signore, Marco Pietrosanto, Manuela Helmer-Citterich, Matteo Pallocca, Maurizio Fanciulli, Tiziana Bruno, Francesca De Nicola, Giacomo Corleone, Anna Di Benedetto, Cristiana Ercolani, Edoardo Pescarmona, Laura Pizzuti, Francesco Guidi, Francesca Sperati, Sara Vitale, Daniele Macchia, Massimo Spada, Giovanna Schiavoni, Fabrizio Mattei, Adele De Ninno, Luca Businaro, Valeria Lucarini, Laura Bracci, Eleonora Arico, Giovanna Ziccheddu, Francesco Facchiano, Stefania Rossi, Massimo Sanchez, Alessandra Boe, Mauro Biffoni, Ruggero De Maria, Ilio Vitale, Antonella Sistigu
Summary: The study demonstrates that type I interferons can promote the formation of cancer stem cells by upregulating the chromatin remodeling factor KDM1B. Inhibition of KDM1B could potentially prevent stem cell expansion and increase the long-term benefit of therapy.
Review
Oncology
Luciano Castiello, Laura Santodonato, Mariarosaria Napolitano, Davide Carlei, Enrica Montefiore, Domenica Maria Monque, Giuseppina D'Agostino, Eleonora Arico
Summary: Despite success in hematology, chimeric antigen receptor T cell therapies have shown limited progress in treating solid tumors. Multiple factors, including the heterogeneity of solid tumors and difficulties in identifying reliable tumor antigens, contribute to this slower pace. Currently, different CAR therapies are being investigated, with varying construction types, engineered cells, and additional signals to overcome solid tumor barriers. Novel approaches are also being developed to address limitations seen in approved therapies. This review analyzes the advantages and challenges of these different approaches, considering both scientific evidence and manufacturing/regulatory issues for further clinical development.
Review
Biochemistry & Molecular Biology
Ambra Natalini, Sonia Simonetti, Carmel Sher, Ugo D'Oro, Adrian C. Hayday, Francesca Di Rosa
Summary: Anti-SARS-CoV-2 memory CD8 T cells induced by vaccination play an important role in mitigating the COVID-19 pandemic. Vaccination with two or more doses can promote the differentiation of memory CD8 T cells, and the length of inter-dose intervals may influence the magnitude and persistence of CD8 T cell memory.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Meeting Abstract
Immunology
Ambra Natalini, Sonia Simonetti, Giovanna Peruzzi, Miguel Munoz Ruiz, Leticia Monin, Irma Pujol Autonell, Hefin Rhys, Heather M. Long, Adrian C. Hayday, Francesca Di Rosa
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)