Article
Biochemistry & Molecular Biology
Rose Ghemrawi, Mostafa Khair, Shaima Hasan, Raghad Aldulaymi, Shaikha S. AlNeyadi, Noor Atatreh, Mohammad A. Ghattas
Summary: Our study identified three SHP2 inhibitor compounds that significantly reduced proliferation and viability of breast cancer cells. These compounds showed high selectivity and promising binding modes, making them potential starting points for future anticancer drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Rongxing Liu, Yujie Sun, Jeremy Berthelet, Linh-Chi Bui, Ximing Xu, Mireille Viguier, Jean-Marie Dupret, Frederique Deshayes, Fernando Rodrigues Lima
Summary: This study analyzed the molecular and functional characteristics of three PTP1B mutations identified in primary mediastinal B cell lymphoma (PMBCL) patients. The results showed that these mutations led to PTP1B mutants with extremely low intrinsic tyrosine phosphatase activity and alterations in overall protein stability and flexibility of the active site loops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Shujing Gao, Yao Tan, Haibin Han, Na Guo, Haiyan Gao, Linbo Xu, Kejian Lin
Summary: This study revealed that field populations of Oedaleus asiaticus have developed low levels of resistance to beta-cypermethrin and matrine, but remain susceptible to azadirachtin, indicating a tendency towards resistance. Biochemical assays suggested that elevated CarE and GST activities may play a key role in the development of beta-cypermethrin resistance, while P450 is important in metabolizing matrine and azadirachtin.
JOURNAL OF INSECT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Emanuele Bellacchio
Summary: The localization and activity of SHP2 tyrosine phosphatase are regulated by the binding of phosphotyrosine peptides to the tandem SH2 domains. The N-SH2 domain can inhibit the enzyme's activity by blocking the catalytic site, but this inhibition can be relieved by binding of phosphotyrosine peptides. A molecular dynamics simulation study reveals that the closed conformation of the self-inhibited phosphatase is stabilized by the interaction between the N-SH2 domain and a conserved peptide portion in PTPN11 exon 6.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Preeti Choudhary, Stephen Anyango, John Berrisford, James Tolchard, Mihaly Varadi, Sameer Velankar
Summary: More than 61,000 proteins have correspondence between their amino acid sequence (UniProtKB) and 3D structures (PDB) through the SIFTS resource. SIFTS incorporates residue-level annotations from various biological resources and is maintained separately from the structure data in the PDB archive.
Article
Chemistry, Multidisciplinary
Jinmin Miao, Jiajun Dong, Yiming Miao, Yunpeng Bai, Zihan Qu, Brenson A. Jassim, Bo Huang, Quyen Nguyen, Yuan Ma, Allison A. Murray, Jinyue Li, Philip S. Low, Zhong-Yin Zhang
Summary: In this study, researchers discovered the first highly potent and selective TC-PTP degrader called TP1L, which can induce degradation of TC-PTP and enhance T-cell signaling and tumor killing efficacy.
Review
Cell Biology
Francisca Cornejo, Bastian I. Cortes, Greg M. Findlay, Gonzalo I. Cancino
Summary: Protein phosphatases are key regulators of signal transduction, with the LAR-RPTP subfamily playing an important role in neurobiology, from neurodevelopment to brain disorders. The LAR-RPTP subfamily consists of three members in vertebrates and is involved in various brain functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Alexander Drilon, Manish R. Sharma, Melissa L. Johnson, Timothy A. Yap, Shirish Gadgeel, Dale Nepert, Gang Feng, Micaela B. Reddy, Allison S. Harney, Mohamed Elsayed, Adam W. Cook, Christina E. Wong, Ronald J. Hinklin, Yutong Jiang, Eric N. Brown, Nickolas A. Neitzel, Ellen R. Laird, Wen - Wu, Anurag Singh, Ping Wei, Keith A. Ching, John J. Gaudino, Patrice A. Lee, Dylan P. Hartley, S. Michael Rothenberg
Summary: PF-07284892, an allosteric SHP2 inhibitor, combined with various oncogenic driver inhibitors, overcame bypass-signaling-mediated resistance in clinical settings. This combination therapy showed significant tumor and circulating tumor DNA responses, extending the duration of overall clinical benefit in patients with targeted therapy resistance in lung, colorectal, ovarian, and pancreatic cancer.
Article
Virology
Hong-Shuai Gao, Rong-Min Hu, Ze-Hua Wang, Xi-Qian Ye, Xiao-Tong Wu, Jian-Hua Huang, Zhi-Zhi Wang, Xue-Xin Chen
Summary: In this study, a PTP gene, CvBV_12-6, derived from CvBV was characterized and found to negatively regulate the immune response in the host P. xylostella. It suppressed the phenoloxidase activity and antibacterial abilities of hemocytes, as well as reduced the host's melanization and viability.
Article
Biochemistry & Molecular Biology
Yan Ma, Haibo Zhang, Cheng Chen, Lixin Liu, Ting Ding, Ying Wang, Dachang Ma, Xiaoling Ling, Xiaohua Chen, Jianping Li, Guansheng Zhong, Guoqing Ru, Lei Zhang, Jianming Tang
Summary: This study reveals that TRIM32 is upregulated and negatively associated with survival of TNBC patients. Radiotherapy enhances the expression of TRIM32, while depletion of TRIM32 reduces TNBC radioresistance. Mechanistically, radiotherapy promotes the association between TRIM32 and nuclear STAT3, which suppresses TC45-induced dephosphorylation of STAT3, resulting in increased STAT3 transcriptional activation and TNBC radioresistance. TRIM32 and STAT3 phosphorylation are co-expressed in TNBC tissues and correlated with poor prognosis of TNBC patients.
Article
Biochemistry & Molecular Biology
Sonia Rocha, Mariana Lucas, Vera L. M. Silva, Pedro M. O. Gomes, Artur M. S. Silva, Alberto N. Araujo, Natalia Aniceto, Rita C. Guedes, M. Luisa Corvo, Eduarda Fernandes, Marisa Freitas
Summary: This study evaluated a library of 22 pyrazole compounds against human PTP1B activity, identifying two potent inhibitors and highlighting the importance of additional benzene rings in enhancing PTP1B inhibition. The most active compounds showed selectivity over TCPTP and molecular docking analyses revealed key residue contacts. This study represents a significant step in the design of novel PTP1B inhibitors.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Michael Kovermann, Alessandra Stefan, Chiara Palazzetti, Fabian Immler, Fabrizio Dal Piaz, Luca Bernardi, Valentina Cimone, Maria Laura Bellone, Alejandro Hochkoeppler
Summary: The low-molecular weight protein tyrosine phosphatase (MptpA) of Mycobacterium tuberculosis plays a crucial role in inhibiting phagosome-lysosome fusion and contributing to bacterial pathogenicity. The enzyme undergoes conformational changes and decreased catalytic efficiency in acidic pH conditions. Competitive inhibitors of MptpA are more effective at acidic pH values.
Article
Cell Biology
Zhe Qian, Dongyan Song, Jonathan J. Ipsaro, Carmelita Bautista, Leemor Joshua-Tor, Johannes T. -H. Yeh, Nicholas K. Tonks
Summary: In this study, the researchers generated a novel monoclonal antibody, RD-43, which targeted the ectodomain of PTPRD to inhibit its function and promote its degradation. Treatment with RD-43 was found to suppress SRC signaling required for breast cancer cell invasion. These findings suggest that antibodies targeting extracellular segments of receptor protein tyrosine phosphatases may have therapeutic potential.
GENES & DEVELOPMENT
(2023)
Article
Cell Biology
Stephanie M. Stanford, Meghan Collins, Michael A. Diaz, Zachary J. Holmes, Paul Gries, Matthew R. Bliss, Alessia Lodi, Vida Zhang, Stefano Tiziani, Nunzio Bottini
Summary: LMPTP plays a key role in adipocyte differentiation by regulating PDGFRα signaling and affecting multiple pathways involved in fat cell growth, differentiation, and glucose utilization in adipose tissue.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Cell Biology
Michel L. Tremblay
Summary: In this study, a monoclonal antibody targeting PTPRD was identified, which induced dimerization and inhibition of the phosphatase activities of PTPRD and led to its proteolysis by intracellular degradation pathways. This finding opens up a new framework for the clinical manipulation of PTPRD and its closely related family members.
GENES & DEVELOPMENT
(2023)
Article
Biotechnology & Applied Microbiology
Feng Wang, Xiangqun Nong, Kun Hao, Ni Cai, Guangjun Wang, Shaofang Liu, Hidayat Ullah, Zehua Zhang
Article
Entomology
Beibei Li, Hongmei Li, Ye Tian, Nazir Ahmed Abro, Xiangqun Nong, Zehua Zhang, Guangjun Wang
Summary: The study demonstrated the crucial role of Lmserpin1 gene in the humoral immunity of insects, enhancing immune responses in Locusta migratoria manilensis and inhibiting infection by Metarhizium anisopliae.
Article
Entomology
Shuang Li, Jie Wang, Dongnan Cui, Kun Hao, Jun Chen, Xiongbing Tu, Zehua Zhang
Summary: This study identified PTP1B, PTK, and Prx V as upstream modulators regulating egg diapause through the insulin-like signaling pathway. Under short photoperiod conditions, PTP1B induces egg diapause, while PTK inhibits it. Moreover, Prx V functions as a negative regulator of NADPH oxidizing reaction, reducing ROS production and NADPH-OX activity.
Article
Food Science & Technology
Kashan Ahmad, Zia-ud Din, Hidayat Ullah, Qin Ouyang, Sumayya Rani, Ibadullah Jan, Mukhtar Alam, Ziaur Rahman, Tariq Kamal, Saqib Ali, Sheraz Ahmad Khan, Durri Shahwar, Farhana Gul, Muhammad Ibrahim, Taufiq Nawaz
Summary: An easy and simple approach to fabricate nano-composite packaging films by reinforcing starch molecules with cellulose nanofibers derived from unripe banana peel has been developed. The addition of cellulose nanofibers into the starch matrix improves the mechanical and thermal stability of the films, as well as reducing water solubility and water vapor permeability. The incorporation of nano-particles significantly enhances the mechanical properties of the films.
Article
Biology
Muhammad Saeed, Tanveer Ahmad, Mukhtar Alam, Laila A. Al-Shuraym, Nazeer Ahmed, Mohammed Ali Alshehri, Hidayat Ullah, Samy M. Sayed
Summary: The Peach Fruit Fly (PFF) and the Melon Fruit Fly (MFF) are severe insect pests for fruits and vegetables. The study found that Sponge Gourd and Bitter Gourd were preferred hosts for these flies, while Banana and Orange were preferred fruits. The preference of intact and infested fruits and vegetables also varied for the two fly species.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Entomology
Yunping Wang, Shuang Li, Guilin Du, Gao Hu, Yunhui Zhang, Xiongbing Tu, Zehua Zhang
Summary: Airflow, particularly wind shear, is crucial for the long-distance migration of O. decorus asiaticus. The prevailing northwest wind plays a significant role in its successful migration from Mongolia to China. Analysis of meteorological conditions and insect prevalence suggests that the grasshopper outbreaks in China may originate from Mongolia. These findings provide valuable insights for predicting and reducing outbreaks in China.
Article
Plant Sciences
Jawad Ali, Ibadullah Jan, Hidayat Ullah, Nazeer Ahmed, Mukhtar Alam, Rafi Ullah, Mohamed El-Sharnouby, Hosny Kesba, Mustafa Shukry, Samy Sayed, Taufiq Nawaz
Summary: This study evaluated the role of Ascophyllum nodosum extract (ANE) in improving drought tolerance of okra. The results show that ANE application can reduce growth disturbances and visible growth reduction in okra under drought stress, and promote chlorophyll and antioxidant activity.
Article
Virology
Jin Li, Qiaoxia Shang, Yanqi Liu, Wenting Dai, Xin Li, Shuhua Wei, Guixin Hu, Mark Richard McNeill, Liping Ban
Summary: This study investigated the virus diversity in major alfalfa-growing areas in China and identified 18 alfalfa viruses, including two new ones. The role of Odontothrips loti in alfalfa virus transmission was confirmed, and the dynamic changes in the virus during different developmental stages of thrips were clarified.
Article
Microbiology
Ni Cai, Rong Liu, Duozi Yan, Neng Zhang, Kaihui Zhu, Daogang Zhang, Xiangqun Nong, Xiongbing Tu, Zehua Zhang, Guangjun Wang
Summary: In this study, 13 CFEM genes from the entomopathogen Metarhizium anisopliae were cloned and identified. These genes were analyzed for their sequence features, phylogenetic relationships, and expression patterns. The study found that six potential effector proteins with varying structures and subcellular localizations in host cells may play important roles in the interaction between M. anisopliae and plants.
Article
Biotechnology & Applied Microbiology
Duozi Yan, Ni Cai, Xiangqun Nong, Guangjun Wang, Qinglei Wang, Hidayat Ullah, Xiongbing Tu, Zehua Zhang
Summary: The fungi Metarhizium anisopliae and Trichoderma harzianum play important roles in biocontrol, plant growth promotion, and survival. This study analyzed the transcriptomic changes in peanut roots inoculated with these fungi, revealing differentially expressed genes involved in diverse pathways. The findings suggest that these fungi can regulate hormone signal transduction and metabolic pathways for successful colonization, and have specific effects on plant immune response and nutritional metabolism.
BIOLOGICAL CONTROL
(2022)
Article
Biochemistry & Molecular Biology
Wenting Dai, Bin Zhu, Marcel van Tuinen, Tao Zhu, Dongliang Shang, Pedro Almeida, Pei Liang, Hidayat Ullah, Liping Ban
Summary: This study analyzed the genetic mechanisms underlying pesticide resistance in diamondback moths, and identified the CYP6B6-like gene as being involved in the resistance. The study also demonstrated that differential gene expression contributes to the development of resistance. The findings have important implications for the development of effective pest control measures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Xiang Zhang, Qiong Wu, Jianing Mu, Zunqi Chao, Qi He, Ting Gao, Chuan Wang, Mark R. McNeill, Zhaozhi Lu
Summary: Maximizing the combination of biological control by natural enemies and plant resistance is a key strategy for controlling aphids in various crop systems. In our study, some alfalfa cultivars were found to have resistance to the pea aphid, Acyrthosiphon pisum, a dominant pest in alfalfa fields in northwestern China. Moreover, natural enemies were able to significantly suppress this aphid, regardless of the resistance level of the nine cultivars tested.
Article
Biochemistry & Molecular Biology
Ni Cai, Xiangqun Nong, Rong Liu, Mark Richard McNeill, Guangjun Wang, Zehua Zhang, Xiongbing Tu
Summary: Metarhizium anisopliae, an entomopathogenic fungus, has been found to enhance plant growth and resistance as an endophyte. The interactions between this fungus and host plants involve CFEM proteins, which regulate plant immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Green & Sustainable Science & Technology
Jawad Ali, Ibadullah Jan, Hidayat Ullah, Shah Fahad, Shah Saud, Muhammad Adnan, Baber Ali, Ke Liu, Matthew Tom Harrison, Shah Hassan, Sunjeet Kumar, Muhammad Amjad Khan, Muhammad Kamran, Mona S. Alwahibi, Mohamed S. Elshikh
Summary: Drought stress restricts the growth of okra by disrupting its biochemical and physiological functions. Selenium application can significantly improve okra's tolerance to drought and alleviate drought-induced biochemical disturbances. Particularly, 3 mg L-1 foliar selenium application can enhance chlorophyll and antioxidant contents, reduce oxidative stress damage, and improve plant tolerance to drought stress.