4.7 Article

Toxicity and developmental effect of cucurbitacin E fromCitrullus colocynthisL. (Cucurbitales: Cucurbitaceae) againstSpodoptera lituraFab. and a non-target earthwormEisenia fetidaSavigny

Journal

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 27, Issue 19, Pages 23390-23401

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s11356-019-04438-1

Keywords

Toxicity; FT-IR; GC-MS; Mortality; Antifeedant; Earth worm

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Pest management with natural botanical insecticides is a significant implementation for the sustainability of agroecosystem by reducing the unnecessary risk from the inputs of synthetic insecticides. In this research, we isolated the bioactive compound cucurbitacin E fromCitrullus colocynthis(L.) Schrad, and their toxicological effects were screened against different larval instars ofSpodoptera litura. The bioactive compound cucurbitacin E was chemically characterized through TLC, FT-IR, and NMR analyses. The larval mortality bioassay revealed that the larvae exposed to cucurbitacin E at the discriminating dose of 50 ppm display higher mortality rate against second (93.8%), third (86.4%), and fourth (73.2%) instar respectively. The lethal concentrations (LC(50)and LC90) was detected as 15.84 and 67.60 ppm for third instar respectively. The sub-lethal concentration of cucurbitacin E (2, 4, and 6 ppm) intentionally altered the percentage of survival, pupation, fecundity, and egg hatchability ofS. litura. Moreover, antifeedant activity of cucurbitacin E was analyzed using choice-based test. In addition, we found the toxic effects of cucurbitacin E (50 and 100 ppm) and chemical pesticides (cypermethrin and monocrotophos) against terrestrial beneficial earthwormEisenia fetida, and the result revealed that cucurbitacin E has no harmful effect on non-target organism. Hence, the present study reveals that cucurbitacin E might be a part of a new biorational product alternative to synthetic pesticides.

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