Article
Chemistry, Multidisciplinary
Anish R. Roy, Wei Zhang, Zeinab Jahed, Ching-Ting Tsai, Bianxiao Cui, W. E. Moerner
Summary: The topography of the plasma membrane has a strong influence on cellular processes. Recent studies have used 3D nanostructures to study the effects of membrane curvature on protein distribution. In this study, a method combining silicone-oil immersion objective and 3D double-helix point spread function microscopy is introduced to investigate the nanoscale distribution of proteins at the nano-bio interface.
Article
Chemistry, Multidisciplinary
Teodora Andrian, Pietro Delcanale, Silvia Pujals, Lorenzo Albertazzi
Summary: The functionalization of nanoparticles with functional moieties is important for achieving cell targeting in nanomedicine. The correlation between size and ligand number in nanoparticles is crucial for understanding structure-activity relations. A new method combining super-resolution microscopy and transmission electron microscopy, called super-resCLEM, has been introduced to accurately measure these parameters and address the issue of heterogeneity in nanoparticle populations.
Article
Biochemistry & Molecular Biology
Shweta Suiwal, Mayur Dembla, Karin Schwarz, Rashmi Katiyar, Martin Jung, Yvonne Carius, Stephan Maxeiner, Marcel A. Lauterbach, C. Roy D. Lancaster, Frank Schmitz
Summary: Unc119 protein mediates transport of myristoylated proteins to the photoreceptor outer segment and is also enriched in photoreceptor synapses. The synaptic ribbon could be involved in the discharge of Unc119-bound lipid-modified proteins. Proteins associated with Unc119 may play important roles in photoreceptor synapses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Review
Chemistry, Physical
Peter D. Dahlberg, W. E. Moerner
Summary: srCryoCLEM is an emerging method that combines the advantages of super-resolution fluorescence microscopy and cryogenic electron tomography, providing higher resolution and more informative data. The method is still in its infancy with many technical challenges to be overcome.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 72
(2021)
Article
Multidisciplinary Sciences
Yicong Wu, Xiaofei Han, Yijun Su, Melissa Glidewell, Jonathan S. Daniels, Jiamin Liu, Titas Sengupta, Ivan Rey-Suarez, Robert Fischer, Akshay Patel, Christian Combs, Junhui Sun, Xufeng Wu, Ryan Christensen, Corey Smith, Lingyu Bao, Yilun Sun, Leighton H. Duncan, Jiji Chen, Yves Pommier, Yun-Bo Shi, Elizabeth Murphy, Sougata Roy, Arpita Upadhyaya, Daniel Colon-Ramos, Patrick La Riviere, Hari Shroff
Summary: Researchers have enhanced the performance of confocal microscopy using four approaches: developing compact line scanners, combining multi-view imaging, adapting techniques from structured illumination microscopy, and utilizing deep learning. They successfully improved imaging resolution, reduced phototoxicity, and demonstrated these advancements on over 20 different samples.
Review
Biochemistry & Molecular Biology
Charles Bond, Adriana N. Santiago-Ruiz, Qing Tang, Melike Lakadamyali
Summary: Since its initial demonstration in 2000, there have been significant technological advancements in far-field super-resolution light microscopy. These developments have allowed for unprecedented visualization of the inner workings of cells. This review discusses the technical details of common implementations of super-resolution microscopy and highlights recent promising advances in the field.
Article
Optics
Luis E. Villegas-Hernandez, Vishesh Dubey, Mona Nystad, Jean-Claude Tinguely, David A. Coucheron, Firehun T. Dullo, Anish Priyadarshi, Sebastian Acuna, Azeem Ahmad, Jose M. Mateos, Gery Barmettler, Urs Ziegler, Asa Birna Birgisdottir, Aud-Malin Karlsson Hovd, Kristin Andreassen Fenton, Ganesh Acharya, Krishna Agarwal, Balpreet Singh Ahluwalia
Summary: This study introduces a photonic chip as a high-throughput microscopy platform for super-resolution imaging of histological samples. It demonstrates diverse imaging capabilities and paves the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Neurosciences
Cristina Roman-Vendrell, Audrey T. Medeiros, John B. Sanderson, Haiyang Jiang, Tim Bartels, Jennifer R. Morgan
Summary: Alpha-synuclein accumulates in neurodegenerative diseases, affecting synaptic function and leading to motor, cognitive, and autonomic dysfunction. Different molecular species of alpha-synuclein result in distinct trafficking defects at synapses, with multimeric alpha-synuclein causing intracellular vesicle trafficking impairment.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Cell Biology
James H. Felce, Lucia Parolini, Erdinc Sezgin, Pablo F. Cespedes, Kseniya Korobchevskaya, Mathew Jones, Yanchun Peng, Tao Dong, Marco Fritzsche, Dirk Aarts, John Frater, Michael L. Dustin
Summary: The study reveals the spatiotemporal organization of GPCRs within the synapse of T cells, focusing on the contribution of CXCR4 in T cell activation. These GPCRs show a similar pattern of organization in the synapse to CXCR4, providing insights into how they contribute to T cell activation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yun-Bin Lee, Minkyo Jung, Jeesoo Kim, Afandi Charles, Wanda Christ, Jiwoong Kang, Myeong-Gyun Kang, Chulhwan Kwak, Jonas Klingstrom, Anna Smed-Sorensen, Jong-Seo Kim, Ji Young Mun, Hyun-Woo Rhee
Summary: This study used a super-resolution proximity labeling (SR-PL) method to investigate the interactions between SARS-CoV-2 and host cells. The results showed that RNF5 interacts with ORF3a and promotes its protein degradation, leading to a reduction in SARS-CoV-2 infection rate.
Article
Cell Biology
Juliana E. Gentile, Melissa G. Carrizales, Anthony J. Koleske
Summary: This article reviews the historical understanding of synaptic actin and highlights its key role in neuronal function. It discusses the organization and regulation of synaptic actin, as well as genetic variants related to neurodevelopmental and psychiatric disorders.
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Dokyung Jeong, Doory Kim
Summary: The combination of correlative super-resolution fluorescence microscopy (SRM) and electron microscopy (EM) is an effective hybrid technique that allows the simultaneous acquisition of spatial locations of specific molecules and the context of cellular ultrastructure. Although there are challenges in integrating these two microscopy techniques, extensive research has led to significant improvements and widespread applications.
MOLECULES AND CELLS
(2022)
Review
Biochemistry & Molecular Biology
Jon Ander Nieto-Garai, June Olazar-Intxausti, Itxaso Anso, Maier Lorizate, Oihana Terrones, Francesc-Xabier Contreras
Summary: Interorganelle membrane contact sites (MCS) are areas of close vicinity between the membranes of two organelles that are maintained by protein tethers. The study of MCS is important due to its implication in various biological functions. Super-resolution fluorescence microscopy (SRFM) has emerged as a powerful tool for studying MCS. This review explores the characteristics and biological functions of MCS, summarizes different approaches for studying them, and focuses on the use of SRFM techniques in MCS research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Alena Maul, Antje Kathrin Huebner, Nicola Strenzke, Tobias Moser, Rudolf Ruebsamen, Sasa Jovanovic, Christian A. Huebner
Summary: This study reveals the importance of Kolliker's organ activity for the refinement of central auditory connectivity. The study also suggests the involvement of TMEM16A in the propagation of Ca2+ waves.
Article
Chemistry, Multidisciplinary
Aida Garrido-Charles, Antoine Huet, Carlo Matera, Anupriya Thirumalai, Jordi Hernando, Amadeu Llebaria, Tobias Moser, Pau Gorostiza
Summary: Artificial control of neuronal activity is crucial for studying neural circuits and restoring neural functions. In this study, researchers developed fast photoswitchable ligands of glutamate receptors (GluRs) to enable direct and reversible control of intact neurons using visible light pulses. These new photoswitchable ligands successfully induced photocurrents in untransfected auditory neurons, offering a drug-based approach for optical stimulation without the need for genetic manipulation. This method shows potential for spatiotemporal control of neural activities in the brain.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Anatomy & Morphology
Homero L. Cantu-Guerra, Michael R. Papazian, Anna L. Gorsky, Nathalie S. Alekos, Adam Caccavano, Nare Karagulyan, Jakob Neef, Stefano Vicini, Tobias Moser, Thomas M. Coate
Summary: SEMA3A may regulate the excitability of SGNs in the cochlea, which could influence the morphology and synaptic arrangement of type I SGNs.
DEVELOPMENTAL DYNAMICS
(2023)
Article
Clinical Neurology
Artur Mittring, Tobias Moser, Antoine Tarquin Huet
Summary: Optogenetic control allows for innovative approaches to restore function in diseased sensory and motor systems. Customization of channelrhodopsins, emitters, and coding strategies is necessary to achieve success in clinical translation. This study provides a framework for parametrizing optogenetic neural control and applies it to the auditory pathway.
Article
Chemistry, Physical
Christian Helke, Markus Reinhardt, Markus Arnold, Falk Schwenzer, Micha Haase, Matthias Wachs, Christian Gossler, Jonathan Goetz, Daniel Keppeler, Bettina Wolf, Jannis Schaeper, Tim Salditt, Tobias Moser, Ulrich Theodor Schwarz, Danny Reuter
Summary: Improved hearing restoration through optical cochlear implants, which excite optogenetically modified spiral ganglion neurons via an optical pulse, is achieved by using flexible polymer-based waveguide probes. These probes are fabricated using micromachining processes and are capable of guiding light with low intensity loss.
Article
Cell Biology
Marion Weber-Boyvat, Jana Kroll, Thorsten Trimbuch, Vesa M. Olkkonen, Christian Rosenmund
Summary: This research identified a dual function of ORP2, serving as a physiological regulator of synaptic cholesterol content and a regulator of neuronal exocytosis.
Article
Neurosciences
Victoria Hunniford, Robert Kuehler, Bettina Wolf, Daniel Keppeler, Nicola Strenzke, Tobias Moser
Summary: Hearing-impaired individuals using electrical cochlear implants (eCIs) expressed a desire for improved speech comprehension, music appreciation, and more natural sound impression. They showed willingness to engage with new technologies for improved hearing restoration. However, they had concerns about receiving an implant that had not been evaluated in a human clinical trial.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Medicine, Research & Experimental
Maria Zerche, Christian Wrobel, Kathrin Kusch, Tobias Moser, Thomas Mager
Summary: Optogenetic neurostimulation, using f-Chrimson-TSKir2.1 as a candidate, shows promise for sensory restoration and can be a potential alternative to electrical stimulation methods. Although removing the C-terminal fluorescent protein leads to a reduction in photocurrent amplitude, adjusting the dose of the adenoassociated virus vector and using the Kir2.1 trafficking sequence can restore optogenetic stimulation efficacy with minimal neural loss after a few months. This research holds significance for the clinical translation of optogenetic neurostimulation, particularly in the development of optical cochlear implants.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Review
Biochemistry & Molecular Biology
Tobias Moser, Nare Karagulyan, Jakob Neef, Lina Maria Jaime Tobon
Summary: This review discusses the mechanisms behind processing different sound pressures and intensities in the auditory system, as well as the neural decomposition and coding of sound intensity information.
Article
Biochemistry & Molecular Biology
Susann Michanski, Rohan Kapoor, Anna M. Steyer, Wiebke Moebius, Iris Frueholz, Frauke Ackermann, Mehmet Gueltas, Craig C. Garner, F. Kent Hamra, Jakob Neef, Nicola Strenzke, Tobias Moser, Carolin Wichmann
Summary: In this study, the role of piccolino in the structure and function of inner hair cell synapses was investigated using piccolo gene-trap mutant rats. The findings suggest that piccolino is essential for normal hearing and contributes to the organization of the active zone in inner hair cells.
Article
Cell Biology
Susann Michanski, Timo Henneck, Mohona Mukhopadhyay, Anna M. Steyer, Paola Aguei Gonzalez, Katharina Grewe, Peter Ilgen, Mehmet Gueltas, Eugenio F. Fornasiero, Stefan Jakobs, Wiebke Moebius, Christian Vogl, Tina Pangrsic, Silvio O. Rizzoli, Carolin Wichmann
Summary: In mammals, the sensory hair cells of the vestibular labyrinth encode spatial orientation through synaptic activity. However, the mechanism and morphological changes underlying vestibular deficits associated with aging are not well understood. We used electron microscopy, confocal and super-resolution light microscopy, and metabolic imaging to study the ultrastructure of ribbon-type active zones in murine utricles. Our findings suggest that aging leads to dramatic structural alterations in vestibular hair cells' ribbon-type active zones.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Multidisciplinary Sciences
Lakshay Khurana, Tamas Harczos, Tobias Moser, Lukasz Jablonski
Summary: Hearing loss is a common sensory deficit, and optical cochlear implants have the potential to overcome the limitations of electrical implants by using light stimulation.