Article
Biochemical Research Methods
Yufeng Zhang, Jiayu Zeng, Chao Huang, Bixue Zhu, Qianjun Zhang, Dongmei Chen
Summary: This study utilized Ag@SCNPs nanoparticles as an enhanced substrate to eliminate impurities interference in DNA signals, directly obtaining intrinsic structural information of ssDNA. The base-stacking rules of ssDNA in a solution environment were explored for the first time, identifying mutation sites and obtaining Raman spectra with high stability, repeatability, and high signal-to-noise ratio. The method is simple, fast, accurate, and nondestructive, with potential applications in medical diagnosis and genetics research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Yuanfeng Pang, Qing Li, Chongwen Wang, Shuai Zhen, Zhiwei Sun, Rui Xiao
Summary: By combining CRISPR-Cas12a and SERS technology, the sensitivity and specificity of LFA nucleic acid detection have been improved, allowing for direct quantification of HIV-1 dsDNA in less than 1 hour and recognition of HIV-1 single-base drug resistance mutations. This paper-based CRISPR-SERS strip has great potential for point-of-care testing in resource-poor or non-laboratory environments.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Multidisciplinary Sciences
Wonkyoung Lee, Byoung-Hoon Kang, Hyunwoo Yang, Moonseong Park, Ji Hyun Kwak, Taerin Chung, Yong Jeong, Bong Kyu Kim, Ki-Hun Jeong
Summary: The spread spectrum SERS method allows rapid quantification of neurotransmitters with high temporal resolution and signal-to-noise ratio improvement. It enables unlabelled detection of neurotransmitters without Raman reporters, showing potential for early diagnosis of neurological disorders and biosensing applications.
NATURE COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Sonja Schmid, Pierre Stoemmer, Hendrik Dietz, Cees Dekker
Summary: The NEOtrap, formed by a DNA-origami sphere docked onto a passivated solid-state nanopore, allows the trapping and observation of single proteins for hours with high sensitivity and resolution. It can distinguish between nucleotide-dependent protein conformations and is a promising tool for studying the molecular kinetics underlying protein function.
NATURE NANOTECHNOLOGY
(2021)
Article
Optics
Shi Bai, Xueli Ren, Kotaro Obata, Yoshihiro Ito, Koji Sugioka
Summary: This paper introduces the application of liquid interface assisted SERS (LI-SERS) method, which has high sensitivity and versatility for label-free trace detection of bio-molecules. The ultrahigh sensitivity of the LI-SERS method is demonstrated through DNA discrimination and quantitative detection of beta-Amyloid. These results suggest the potential application of LI-SERS method in early-stage diagnosis of diseases such as virus infection and Alzheimer's disease.
OPTO-ELECTRONIC ADVANCES
(2022)
Review
Chemistry, Multidisciplinary
Eleonora Macchia, Fabrizio Torricelli, Paolo Bollella, Lucia Sarcina, Angelo Tricase, Cinzia Di Franco, Ronald Osterbacka, Zsolt M. Kovacs-Vajna, Gaetano Scamarcio, Luisa Torsi
Summary: Nanometric bioelectronic transducing surfaces have been the main method for detecting single molecules, but are unsuitable for assays at concentrations below the nanomolar level. However, some large-area transistors have shown detection limits lower than femtomolar.
Article
Chemistry, Analytical
Yuanxun Gong, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Ning Xin, Fenglei Gao
Summary: In this study, a label-free surface-enhanced Raman scattering sensor was designed for rapid detection of apolipoprotein A4. The method showed advantages of simplicity, rapidness, and high selectivity, meeting the clinical requirements and demonstrating great potential for application.
Article
Chemistry, Analytical
Jia-Wei Tang, Jing-Wen Lyu, Jin-Xin Lai, Xue-Di Zhang, Yang-Guang Du, Xin-Qiang Zhang, Yu-Dong Zhang, Bin Gu, Xiao Zhang, Bing Gu, Liang Wang
Summary: In this study, a novel method for rapid and accurate discrimination of Shigella spp. was developed using label-free SERS coupling with a multiscale deep-learning method. Unique barcodes were generated by SERS spectral deconvolution, revealing subtle differences in molecular composition between Shigella spp. Four supervised learning models were constructed and assessed for their ability to predict Shigella spp., with the 1DMSCNN model achieving the best performance. A software embedded with the 1DMSCNN model was built to predict raw SERS spectra of Shigella spp.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Ioana Andreea Brezestean, Ana Maria Raluca Gherman, Alia Colnita, Nicoleta Elena Dina, Csilla Muller Molnar, Daniel Marconi, Vasile Chis, Ioan-Leontin David, Simona Cinta-Pinzaru
Summary: The article presents a theoretical characterization of NOD using DFT-vibrational Raman analysis, as well as experimental analysis using the drop-coating deposition Raman technique. Different methods were used for the SERS analysis of NOD, with the most promising results obtained using AgNPs and the Klarite (TM) substrate, which provided the lowest limits of detection. The theoretical computation of NOD's complex vibrational modes was used for the first time to unambiguously assign specific Raman bands.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Veronica Vespini, Simonetta Grilli, Pietro Ferraro, Romina Rega, Heidi Ottevaere, Yunfeng Nie, Pellegrino Musto, Marianna Pannico
Summary: The pyro-electrohydrodynamic jet printing technology was used to fabricate confined assemblies of gold nanoparticles and evaluated for its surface-enhanced Raman spectroscopy (SERS) performance. The results showed strong plasmonic effects, reproducible signals, and affinity with biomolecules, indicating potential applications in biosensing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Umar Azhar, Qazi Ahmed, Saira Ishaq, Zeyad T. Alwahabi, Sheng Dai
Summary: A novel bioassay using Raman-coded antibody supports and surface-enhanced Raman scattering-coded nanotags was developed for accurate detection of multiple analytes from a single measurement, showing great potential for diverse biomedical applications.
Article
Chemistry, Multidisciplinary
Jae-Myoung Kim, Jiyeon Kim, Kyungin Choi, Jwa-Min Nam
Summary: Metal nanostructures with a tunable plasmonic gap are synthesized for various applications. A plasmonic nanostructure with tunable dual nanogaps, called Au dual-gap nanodumbbells (AuDGNs), is designed and synthesized with high yield. These AuDGNs enable label-free on-particle SERS detection assays and show high specificity and sensitivity without the need for Raman dyes.
ADVANCED MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Linjun Cai, Guilin Fang, Jinpin Tang, Qiaomei Cheng, Xiaoxia Han
Summary: This review provides an overview of the application of surface-enhanced Raman spectroscopy (SERS) in structural characterization of biomolecules. It covers topics such as SERS theories and methodology, SERS-active materials and protein-material assemblies, SERS spectroscopy of proteins with and without cofactors, and recent applications in biomarker detection, cell analysis, and pathogen discrimination. The review also discusses the reproducibility and limitations of SERS, and concludes with a discussion of current challenges and future directions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Sheng-Hong Liu, Xiu-Mei Lin, Zhi-Lan Yang, Bao-Ying Wen, Fan-Li Zhang, Yue-Jiao Zhang, Jian-Feng Li
Summary: The study developed a label-free surface-enhanced Raman spectroscopy strategy using silver nanoparticles solution as a substrate to enhance the Raman signal of capsaicin marker molecule in waste oils. The method showed high sensitivity, accessibility, and reliability for the rapid identification of waste oils and has potential applications in the field of food safety.
Article
Chemistry, Analytical
Longjian Huang, Shang Qiu, Zhao Liu, Shengyue Wu, Qianli Tang, Xianjiu Liao, Fenglei Gao
Summary: In this study, a label-free and surface-enhanced Raman spectroscopy method was developed to detect carcinoembryonic antigen (CEA). The method utilized poly adenine-regulated self-assembly methods and toehold-regulated DNA displacement to achieve partial hybridization of the antigen and antibody, and amplified the CEA signals using AuNPs. The method demonstrated high sensitivity and satisfactory stability, and showed good analytical performance in determining serum CEA.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Physical
Zhenyu Zhang, Mu Chen, Lijian Zhan, Fei Zheng, Wei Si, Jingjie Sha, Yunfei Chen
Summary: This article reveals the relationship between the functions of protein molecules and their well-defined three-dimensional structures and dynamics in body fluid. The study uncovers the collective vibrations of elastic poly-alanine alpha-helices and finds distinct vibration patterns over a range of residue numbers. The research also shows that the major vibrational modes of helical structures depend on their lengths and that the helix-coil transition pathway is affected by the alternation of the first-order mode in helices with different lengths. These findings are promising for the de novo design of protein-based materials and artificial biomolecules in clinical treatments.
Article
Chemistry, Physical
Wei Si, Runyi Yuan, Gensheng Wu, Yajing Kan, Jingjie Sha, Yunfei Chen, Yin Zhang, Yang Shen
Summary: A heterostructural platform was theoretically designed to control the capture and linear movement of proteins in nanopore protein sequencing by utilizing the different binding affinities of proteins to two isomorphic materials. By strategically driving the peptide along a designed path to the target nanopore using real-time switching of the applied electric field, different ionic current blockades were observed when the peptide compositions were changed, indicating the potential for differentiating different peptides using this platform.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Review
Nanoscience & Nanotechnology
Kabin Lin, Chen Chen, Congsi Wang, Peiyuan Lian, Yan Wang, Song Xue, Jingjie Sha, Yunfei Chen
Summary: Nanopores, as valuable single-molecule sensing tools, have been widely applied in the detection of various biological molecules. With the development of semiconductor fabrication techniques and materials science, nanopore preparation and modification technologies have made significant progress, providing us with more options.
Editorial Material
Chemistry, Physical
Zhenyu Zhang, Mu Chen, Lijian Zhan, Fei Zheng, Wei Si, Jingjie Sha, Yunfei Chen
Summary: This study theoretically reveals the collective vibrations of elastic poly-alanine alpha-helices, investigates the vibration patterns and dynamic properties of helical structures of different lengths, and highlights the potential value for protein design and clinical treatments.
Article
Nanoscience & Nanotechnology
Chenhan Liu, Zuhuang Chen, Chao Wu, Jing Qi, Menglong Hao, Ping Lu, Yunfei Chen
Summary: This study reports a strategy of electric field-driven crystal symmetry engineering to efficiently modulate thermal conductivity at room temperature, and successfully fabricates a thermal switch prototype based on this strategy.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Physics, Applied
Zhiyong Wei, Li Xiang, Yajing Kan, Yan Zhang, Yunfei Chen
Summary: In this study, the effects of normal load on the friction force of a sliding graphene film were investigated using molecular dynamics simulations. It was found that the excited phonon modes couple with the resonant frequency of the frictional system, and the enhancement of normal load is equivalent to the increase of the resonant frequency. The relative intensity of resonant peaks in the vibrational density of states was identified as a key factor affecting the friction force.
JOURNAL OF APPLIED PHYSICS
(2022)
Article
Chemistry, Physical
Jian Ma, Zhenyu Zhang, Xuan Gu, Kun Li, Deyu Li, Yunfei Chen
Summary: The ion mobility inside narrow nanopores is significantly suppressed by high ion valency, which promotes the formation of ion pairs.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Nanoscience & Nanotechnology
Zhongwu Li, Rahul Prasanna Misra, Yuhao Li, Yun-Chiao Yao, Sidi Zhao, Yuliang Zhang, Yunfei Chen, Daniel Blankschtein, Aleksandr Noy
Summary: Experimental measurements and molecular dynamics simulations show that the Nernst-Einstein relation breaks down in narrow carbon nanotube porins. K+ ion diffusion is three orders of magnitude slower in these channels due to the disintegration of water chains and the formation of K+-water clusters. This study reveals two distinct mechanisms for ion diffusion and electromigration, leading to the breakdown of the overall Nernst-Einstein relation.
NATURE NANOTECHNOLOGY
(2023)
Editorial Material
Multidisciplinary Sciences
Yongkang Wang, Takakazu Seki, Xiaoqing Yu, Chun-Chieh Yu, Kuo-Yang Chiang, Katrin F. Domke, Johannes Hunger, Yunfei Chen, Yuki Nagata, Mischa Bonn
Article
Nanoscience & Nanotechnology
Zhiyong Wei, Dong Han, Qi Wang, Chengdong Sun, Yi Tao, Li Xiang, Yajing Kan, Yan Zhang, Xi Lu, Yunfei Chen
Summary: Applying external vibrations at the resonant frequencies of the frictional system has been an effective approach to suppress friction. However, this study finds that the friction force on the silicon surface can also reach a local minimum when the oscillation frequency of the vertical vibrational excitation equals the washboard frequency with respect to the sliding velocity. Moreover, applying vertical vibrational excitation at the washboard frequency requires much less energy consumption compared to the additional energy consumption at the resonant frequency.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Lijian Zhan, Tonghui Jin, Jiangtao Zhou, Wei Xu, Yunfei Chen, Raffaele Mezzenga
Summary: This study introduces a methodology for noninvasive detection of neurodegenerative diseases by probing the polymorphism of amyloid fibrils using synthetic nanopores. By statistically analyzing translocation events, the structure of hydrated amyloid fibrils can be determined, and abnormal conformations can be detected using ionic current signals. This approach shows potential for rapid diagnosis of early pathological amyloidosis.
Article
Multidisciplinary Sciences
Chenhan Liu, Chao Wu, Xian Yi Tan, Yi Tao, Yin Zhang, Deyu Li, Juekuan Yang, Qingyu Yan, Yunfei Chen
Summary: Doping oxygen atoms in two-dimensional TiS3 nanoribbons can significantly enhance the lattice thermal conductivity. The localized lattice contraction and coupling strength enhancement lead to the suppression of phonon-impurity scattering, improving the phonon thermal transport.
NATURE COMMUNICATIONS
(2023)
Article
Physics, Applied
Chenhan Liu, Yangyang Si, Menglong Hao, Yi Tao, Shiqing Deng, Ping Lu, Chuanwen Zhao, Zuhuang Chen, Gang Zhang, Yunfei Chen
Summary: Electrocaloric cooling is a promising green refrigeration technology with zero global warming potential and high efficiency. In this study, a first-principles method was used to simulate the structural phase transition from antiferroelectric to ferroelectric in the antiferroelectric material PbZrO3 (PZO). By tracking the evolution of phonon entropy and measuring the temperature-dependent polarization, it was revealed that the negative electrocaloric effect in PZO originates from phonon entropy associated latent heat, rather than dipolar entropy. This study proposes a new concept of phonon entropy engineering to enhance the cooling power density by modulating the density of states for low-frequency phonons.
APPLIED PHYSICS REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Yin Zhang, Xiang Lian, Wei Si, Jingjie Sha, Yunfei Chen
Summary: Understanding the origin of ionic conductance modulation in nanopore sensors is crucial for precise measurement of analytes on a single molecule level. Previous models focused on high ionic strength based on geometry and independent of applied voltage. However, our numerical simulations reveal that both electroosmotic flow and ionic concentration polarization are influenced by applied voltage, affecting ionic conductance. DNA translocation enhances the effects, resulting in voltage-dependent conductance blockades. These findings provide valuable insights for nanopore sensor design.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Multidisciplinary
Hanliu Zhao, Yuxin Xue, Yu Zhao, Jiayi Chen, Bo Chang, Hao Huang, Tao Xu, Litao Sun, Yunfei Chen, Jingjie Sha, Beibei Zhu, Li Tao
Summary: In this study, a composition-adjustable 2D bismuth antimonide (Bi100-xSbx) was synthesized using an e-beam evaporation system with homemade targets. Engineering multiscale defects was performed to optimize the thermoelectric performance of the compound. The introduction of Sb alloying drastically decreased the thermal conductivity, while maintaining the electrical conductivity, resulting in an enhanced ZT value of 0.13 for the annealed Bi82Sb18.
MATERIALS HORIZONS
(2023)
