Article
Pharmacology & Pharmacy
Alice Abu Dayyih, Mohamad Alawak, Abdallah M. Ayoub, Muhammad U. Amin, Wael Abu Dayyih, Konrad Engelhardt, Lili Duse, Eduard Preis, Jana Bruessler, Udo Bakowsky
Summary: Optimized Hyp thermosensitive liposomes (HypTSL) demonstrated good colloidal stability and high encapsulation efficiency, exhibiting thermal sensitivity at 42 degrees Celsius. The uptake of Hyp-TSL by MDA-MB-231 cells and cellular reactive oxygen species production were significantly enhanced at 42 degrees Celsius, leading to improved tumor drug availability and therapeutic outcomes.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Nanoscience & Nanotechnology
Alice Abu Dayyih, Bernd Gutberlet, Eduard Preis, Konrad H. Engelhardt, Muhammad Umair Amin, Ahmed M. Abdelsalam, Martina Bonsu, Udo Bakowsky
Summary: This study developed near-infrared (NIR)-activated thermosensitive liposomes (TSL) for antibacterial therapy. The liposomes loaded with NIR dye and water-soluble hypericin showed synergistic photothermal and photodynamic effects, effectively eliminating bacterial infections. The results indicate that the NIR-activated TSL have immense therapeutic potential and good biocompatibility.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Medicinal
Zhaolei Peng, Jing Lu, Kai Liu, Long Xie, Yulin Wang, Chunyan Cai, Dejun Yang, Jingjing Xi, Chunmei Yan, Xiaofang Li, Mingyi Shi
Summary: Hypericin, derived from St. John's wort, has been used in clinical practice for wound healing and depression. This article reviews its pharmacology, pharmacokinetics, and safety, highlighting its excellent anticancer, neuroprotective, and antimicrobial effects when activated by light. The mechanisms of action involve cell death induction, cell cycle inhibition, amine reuptake inhibition, and virus replication inhibition. Although hypericin has low bioavailability, it is safe and effective against various diseases.
PHYTOTHERAPY RESEARCH
(2023)
Review
Microbiology
Camila Barros Galinari, Tiago de Paula Biachi, Renato Sonchini Goncalves, Gabriel Batista Cesar, Eduardo Victor Bergmann, Luis Carlos Malacarne, Erika Seki Kioshima Cotica, Patricia de Souza Bonfim-Mendonca, Terezinha Inez Estivalet Svidzinski
Summary: In recent years, the antimicrobial application of photodynamic therapy (PDT) has gained attention, particularly the antifungal action of hypericin. This review provides a comprehensive overview of the origin, characteristics, and optimal drug delivery methods of hypericin-PDT, as well as the best strategies for antifungal application. It offers a future perspective on the advancements and clinical possibilities of this technique in the treatment of human mycoses.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Oncology
Pollyanna Cristina Vincenzi Conrado, Ana Adelia Vaine, Glaucia Sayuri Arita, Karina Mayumi Sakita, Renato Sonchini Goncalves, Wilker Caetano, Monique de Souza, Mauro Luciano Baesso, Luis Carlos Malacarne, Emanuel Razzolini, Vania Aparecida Vicente, Erika Seki Kioshima, Patricia de Souza Bonfim de Mendonca
Summary: PDT-Hyp as a promising therapy for the clinical treatment of OM, it showed satisfactory results in terms of efficacy and safety, with fungicidal effect and complete permeation through healthy and OM-affected nails.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Mariana Miretti, Cesar G. Prucca, Maria T. Baumgartner, Marisa Martinelli
Summary: Photodynamic therapy is a promising alternative treatment for various pathologies, including cancer. However, the low solubility of photosensitizers is a limitation that needs to be addressed. In this study, a film composed of chitosan, polyvinylpyrrolidone, and liposomes containing Zn-phthalocyanine was synthesized and characterized. The film showed excellent photophysical properties and demonstrated high efficacy in inhibiting cell viability in vitro.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Daphne N. Dorst, Marti Boss, Mark Rijpkema, Birgitte Walgreen, Monique M. A. Helsen, Desiree L. Bos, Louis van Bloois, Gerrit Storm, Maarten Brom, Peter Laverman, Peter M. van der Kraan, Mijke Buitinga, Marije Koenders, Martin Gotthardt
Summary: Macrophages play a key role in rheumatoid arthritis progression, and liposomes loaded with the photosensitizer IRDye700DX can efficiently induce macrophage cell death in inflamed joints, delaying arthritis development. The biodistribution of IRDye700DX-loaded liposomes in mice with arthritis correlated well with arthritis scores, demonstrating the potential for targeted treatment of arthritis.
Article
Biochemistry & Molecular Biology
Camila Barros Galinari, Pollyanna Cristina Vincenzi Conrado, Glaucia Sayuri Arita, Valeria Aparecida Baquetti Mosca, Raquel Cabral Melo, Tiago de Paula Bianchi, Daniella Renata Faria, Karina Mayumi Sakita, Luis Carlos Malacarne, Renato Sonchini Goncalves, Paulo Cesar de Souza Pereira, Gabriel Batista Cesar, Wilker Caetano, Monique de Souza, Raquel da Silva Palacios, Mauro Luciano Baesso, Terezinha Inez Estivalet Svidzinski, Erika Seki Kioshima Cotica, Patricia de Souza Bonfim-Mendonca
Summary: The study evaluated the efficacy of P123-Hy-PDT for dermatophytosis caused by Microsporum canis, demonstrating rapid clinical improvement and reduction in fungal load without causing toxicity.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hui Ling Ma, Laudemir Carlos Varanda, Janice Rodrigues Perussi, Emanuel Carrilho
Summary: In this study, Hypericin-loaded oil-in-water nanoemulsions (NEs) were successfully produced to enhance the solubility and bioavailability of Hypericin. The physical and biological properties of the NEs were characterized, showing significant cytotoxic effects against breast cancer cells. The results suggest that the Hypericin-loaded NEs could be a promising approach for enhancing the efficacy of Hypericin in cancer treatment.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Hui Ling Ma, Ana Carolina Urbaczek, Fayene Zeferino Ribeiro de Souza, Claudia Bernal, Janice Rodrigues Perussi, Emanuel Carrilho
Summary: This study developed a microchip to generate constant shear stress for investigating the efficacy of HyPDT on HUVECs. The microchip showed its potential in promoting the release of VEGF and inducing cell damage and death under shear stress, suggesting its importance in evaluating the therapeutic outcome in preclinical tests.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Polymer Science
Flavia Amanda Pedroso de Morais, Ana Carolina Vieira De Oliveira, Rodolfo Bento Balbinot, Danielle Lazarin-Bidoia, Tania Ueda-Nakamura, Sueli de Oliveira Silva, Katieli da Silva Souza Campanholi, Ranulfo Combuca da Silva Junior, Renato Sonchini Goncalves, Wilker Caetano, Celso Vataru Nakamura
Summary: Biotin, spermine, and folic acid were covalently linked to the F127 copolymer to form a drug delivery system for treating B16F10 cells with HY-loaded PDT. Spectroscopy and spectrophotometric techniques were used to analyze the chemical structures and binders quantification. The hydrophobicity of the binders was found to affect the physicochemical parameters such as critical micelle concentration, critical micelle temperature, size, polydispersity, and zeta potential. The modification with spermine resulted in less changes in the physical and chemical parameters of the micelles.
Article
Pharmacology & Pharmacy
Shijie Luan, Ngoc T. Tran, Hui-Yi Xue, Ho-Lun Wong
Summary: A new liposomal MAL formulation (lipMAL) designed for systemic or intraperitoneal administration was developed in this study to expand the application of MAL to dual intraoperative PDD and PDT of peritoneal carcinomatosis. LipMALs demonstrated controlled MAL release behavior, achieved strong fluorescence in cancer cells, and led to significantly higher fluorescence levels than free MAL groups, showing promising anticancer PDT effects.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Engineering, Biomedical
Eunkyeong Jung, Soonyoung Kwon, Nanhee Song, Nuri Kim, Hanui Jo, Manseok Yang, Sangjun Park, Chunho Kim, Dongwon Lee
Summary: Cancer cells possess antioxidants that hinder the effectiveness of photodynamic therapy (PDT). Additionally, upregulation of pro-angiogenic factors during PDT-induced hypoxia is another challenge. To overcome these obstacles, tumor targeted redox-regulating and antiangiogenic phototherapeutic nanoassemblies (tRAPs) were developed. tRAPs effectively depleted intracellular GSH and suppressed VEGF, enhancing the therapeutic outcomes of PDT.
Review
Polymer Science
Shuaiqi Gan, Yongzhi Wu, Xu Zhang, Zheng Zheng, Min Zhang, Li Long, Jinfeng Liao, Wenchuan Chen
Summary: Phototherapeutic agent-based phototherapies have been proven to be safe for the treatment of malignant tumors. The main modalities include photothermal therapy and photodynamic therapy. However, the phototoxicity of these therapies limits their clinical application. To improve therapeutic performance and minimize side effects, researchers have focused on developing hydrogel-based phototherapy.
Review
Pharmacology & Pharmacy
Sherif Ashraf Fahmy, Hassan Mohamed El-Said Azzazy, Jens Schaefer
Summary: Photodynamic therapy is a promising cancer treatment strategy that utilizes highly reactive oxygen species to kill cancer cells. However, it has shortcomings, leading researchers to develop various nanoplatforms to enhance treatment effectiveness.
Article
Biophysics
Julia Janina Schueer, Christian Woelk, Udo Bakowsky, Shashank Reddy Pinnapireddy
COLLOIDS AND SURFACES B-BIOINTERFACES
(2020)
Article
Chemistry, Multidisciplinary
Julia J. Schueer, Alexej Arndt, Christian Woelk, Shashank R. Pinnapireddy, Udo Bakowsky
Article
Chemistry, Multidisciplinary
Olga Pelikh, Ralph W. Eckert, Shashank Reddy Pinnapireddy, Cornelia M. Keck
Summary: Nanocrystals are a promising formulation for improved drug delivery, targeting the hair follicles and providing long-lasting and efficient dermal drug delivery. Different types of vehicles do not significantly affect the efficacy of nanocrystals' penetration into hair follicles, but they do impact passive dermal penetration. Gels containing humectants can enhance passive penetration effectiveness.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Hirva Shah, Imran Tariq, Konrad Engelhardt, Udo Bakowsky, Shashank Reddy Pinnapireddy
MACROMOLECULAR BIOSCIENCE
(2020)
Article
Materials Science, Biomaterials
Mohamad Alawak, Gihan Mahmoud, Alice Abu Dayyih, Lili Duse, Shashank Reddy Pinnapireddy, Konrad Engelhardt, Ibrahim Awak, Christian Woelk, Alexander M. Koenig, Jana Bruessler, Udo Bakowsky
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Pharmacology & Pharmacy
Christopher Janich, Daniel Ivanusic, Julia Giselbrecht, Elena Janich, Shashank Reddy Pinnapireddy, Gerd Hause, Udo Bakowsky, Andreas Langner, Christian Woelk
Article
Pharmacology & Pharmacy
Michael Raschpichler, Eduard Preis, Shashank Reddy Pinnapireddy, Elias Baghdan, Marcel Pourasghar, Marc Schneider, Udo Bakowsky
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2020)
Article
Pharmacology & Pharmacy
Mohamad Alawak, Alice Abu Dayyih, Gihan Mahmoud, Imran Tariq, Lili Duse, Nathalie Goergen, Konrad Engelhardt, Shashank Reddy Pinnapireddy, Jarmila Jedelsk, Muhannad Awak, Alexander M. Konig, Jana Bruessler, Joerg W. Bartsch, Udo Bakowsky
Summary: The overexpression of ADAM8 in TNBC cells can be exploited for targeted therapy, with specific drug delivery resulting in increased intracellular accumulation and enhanced cytotoxic effects at the cellular target site.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Nanoscience & Nanotechnology
Sajid Ali, Muhammad Umair Amin, Imran Tariq, Muhammad Farhan Sohail, Muhammad Yasir Ali, Eduard Preis, Ghazala Annbreen, Shashank Reddy Pinnapireddy, Jarnnila Jedelska, Jens Schaefer, Udo Bakowsky
Summary: The study aimed to develop an integrated system combining photodynamic and chemotherapeutic approaches for the treatment of multidrug-resistant cancers. The nanoformulations showed good biocompatibility and cytotoxicity against cancer cells.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Dermatology
Olga Pelikh, Shashank R. Pinnapireddy, Cornelia M. Keck
Summary: This study aimed to develop a standard formulation for curcumin and a screening tool for fast discrimination of dermal penetration efficacy. The study utilized ethanolic curcumin solutions as standard formulations and an ex vivo porcine ear model with epifluorescence microscopy for evaluation. The results show that this skin penetration model is a versatile tool for determining dermal penetration efficacy and provides detailed information on the fate of chemical compounds after penetration.
SKIN PHARMACOLOGY AND PHYSIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Eduard Preis, Jan Schulze, Bernd Gutberlet, Shashank Reddy Pinnapireddy, Jarmila Jedelska, Udo Bakowsky
Summary: In 2010, a directive on the protection of animals used for scientific purposes was adopted by the European Parliament and the European Union, with the aim of complete replacement of procedures on live animals for scientific and educational purposes. The directive emphasizes the implementation of the 3Rs principle and promotes the use of the chorioallantoic membrane (CAM) for tumor cell cultivation and therapeutic evaluation of nanoscale drug delivery systems.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Pharmacology & Pharmacy
Jennifer Lehmann, Michael R. Agel, Konrad H. Engelhardt, Shashank R. Pinnapireddy, Sabine Agel, Lili Duse, Eduard Preis, Matthias Wojcik, Udo Bakowsky
Summary: This study explores a new approach for lung cancer treatment, combining liposomes with photodynamic therapy. By comparing different nebulisation technologies, the cytotoxic effects and internalisation of nebulised liposomes on lung cancer cells were investigated. The results suggest that pulmonary application of curcumin-loaded tetraether liposomes may be a promising approach for treating lung cancer.
Article
Pharmacology & Pharmacy
Muhammad Umair Amin, Sajid Ali, Imran Tariq, Muhammad Yasir Ali, Shashank Reddy Pinnapreddy, Eduard Preis, Christian Woelk, Richard D. Harvey, Gerd Hause, Jana Bruessler, Udo Bakowsky
Summary: The study developed a stimuli responsive drug delivery system based on lipid supported mesoporous silica nanoparticles, enabling triggered drug release at the target site while avoiding premature release. Enhanced drug release by FDA approved US-irradiation, along with improved cellular uptake and reduced premature release effects, makes it a suitable candidate for delivering anticancer drugs.
Article
Chemistry, Multidisciplinary
Julia Giselbrecht, Shashank Reddy Pinnapireddy, Fatih Alioglu, Haider Sami, Daniel Sedding, Frank Erdmann, Christopher Janich, Michaela Schulz-Siegmund, Manfred Ogris, Udo Bakowsky, Andreas Langner, Jeroen Bussmann, Christian Woelk
Summary: This study investigates in vivo nucleic acid delivery using simpler models to overcome the limitations of in vitro experiments in predicting in vivo efficacy. The results demonstrate that the OH4:DOPE formulation is effective in transfecting complex tissues and zebra fish embryos.
Article
Cell Biology
Jean Paul Dzoyem, Shashank Reddy Pinnapireddy, Hugues Fouotsa, Jana Bruessler, Frank Runkel, Udo Bakowsky
Summary: This study successfully prepared guttiferone E-loaded liposomes with high entrapment efficiency and demonstrated their in vitro anticancer and anti-inflammatory activities.
MEDIATORS OF INFLAMMATION
(2022)