Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 91, Issue -, Pages 93-100Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2018.10.009
Keywords
Cell-mediated immunity; Th1; Fish disease; Cyprinid; Koi carp; Aquaculture
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Funding
- Program to Disseminate Tenure Tracking System for Promoting Science and Technology from the Japanese Ministry of Education, Culture, Sports, Science and Technology
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In this study, we investigated the immune responses against Mycobacterium gordonae in ginbiuna crucian carp. Cumulative mortality of ginbuna injected with 2.0 x 10(7) CFU of M. gordonae was 50% at 170 days post-infection. CD4-1, CD8 alpha, T-bet and IFN gamma 2 gene expression levels were significantly upregulated in ginbuna injected with 1.9 x 10(8) CFU of M. gordonae at 21 and 28 days post-infection. The CD4-2 level did not change during the experiment. Granulomatous responses consisted of central macrophage accumulation and surrounding lymphocytes, and Ziehl-Neelsen-positive bacteria were observed in the trunk kidney of the challenged fish. Immunohistochemistry using anti-ginbuna IFN gamma s and anti-ginbuna CD4-1 polyclonal antibody revealed that the marginal lymphocytes were positive for CD4-1, and the IFN gamma-producing cells surrounded the mycobacterial cell-laden phagocytes. These results suggest that CD4-1(+) cells and IFN gamma 2 play important roles in the granulomatous inflammation against Mycobacterial infections in teleosts.
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