Article
Microscopy
M. I. Villalba, L. Venturelli, L. Arnal, C. Masson, G. Dietler, M. E. Vela, O. Yantorno, S. Kasas
Summary: In recent years, the coevolution of microorganisms with antibiotics has led to increased bacterial resistance, posing a major global health problem. This study examined the nanomechanical properties of both virulent and avirulent Bordetella pertussis in response to different antibiotics, and found that only virulent cells showed changes in cell elasticity and height after antimicrobial exposure, while avirulent cells remained unaffected. Furthermore, the study revealed different mechanical properties of individual cells compared to those growing in close contact with others, as well as analyzed the presence of the major attachment factor, Filamentous hemagglutinin adhesin (FHA), on the bacterial cell wall under different virulence conditions through Force Spectroscopy.
Article
Microbiology
Kyung Moon, Minji Sim, Chin-Hsien Tai, Kyungyoon Yoo, Charlotte Merzbacher, Sung-Huan Yu, David D. Kim, Jaehyun Lee, Konrad U. Forstner, Qing Chen, Scott Stibitz, Leslie G. Knipling, Deborah M. Hinton
Summary: Through transcriptomic analyses using RNA-seq and ANNOgesic, possible B. pertussis sRNAs were identified and their dependence on BvgAS was determined.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Soraya Matczak, Valerie Bouchez, Pauline Leroux, Thibaut Douche, Nils Collinet, Annie Landier, Quentin Giai Gianetto, Sophie Guillot, Julia Chamot-Rooke, Milena Hasan, Mariette Matondo, Sylvain Brisse, Julie Toubiana
Summary: By comparing the microbiological characteristics and expressed protein profiles between FIM2 and FIM3 fimbrial serotypes and genomic clades, differences were found that may have implications on pathogenesis and epidemiological emergence.
MICROBES AND INFECTION
(2023)
Article
Medicine, General & Internal
Cheryl Keech, Vicki E. Miller, Barbara Rizzardi, Christopher Hoyle, Melinda J. Pryor, Jonathan Ferrand, Ken Solovay, Marcel Thalen, Stephanie Noviello, Peter Goldstein, Andrew Gorringe, Breeze Cavell, Qiushui He, Alex-Mikael Barkoff, Keith Rubin, Camille Locht
Summary: BPZE1, a live attenuated intranasal pertussis vaccine, induces nasal mucosal immunity and functional serum responses. This study demonstrates the potential of BPZE1 to prevent pertussis infection, leading to reduced transmission and diminished epidemic cycles.
Article
Microbiology
Yukihiro Hiramatsu, Koichiro Suzuki, Takashi Nishida, Naoki Onoda, Takashi Satoh, Shizuo Akira, Masahito Ikawa, Hiroko Ikeda, Junzo Kamei, Sandra Derouiche, Makoto Tominaga, Yasuhiko Horiguchi
Summary: The Gram-negative bacterium Bordetella pertussis causes whooping cough, a respiratory disease characterized by paroxysmal coughing. However, the mechanism of coughing has not been extensively studied. In this study, a mouse model was used to reproduce coughing after intranasal inoculation with the bacterium. It was found that lipooligosaccharide, Vag8, and pertussis toxin of the bacteria cooperatively function to cause coughing. Bradykinin and TRPV1 were identified as host factors involved in the coughing mechanism.
Article
Biochemistry & Molecular Biology
Andrei G. Felice, Leonardo N. Q. Santos, Ian Kolossowski, Felipe L. Zen, Leandro G. Alves, Thais C. Rodrigues, Ligia C. S. Prado, Arun K. Jaiswal, Sandeep Tiwari, Fabio M. Miranda, Rommel T. J. Ramos, Vasco Azevedo, Carlo J. F. Oliveira, Leandro J. Benevides, Siomar C. Soares
Summary: Pertussis, caused by Bordetella pertussis, is remerging as a public health problem due to bacterial resistance and decreased vaccine effectiveness. Genetic and docking analysis identified shared genomic features and potential protein targets for new vaccines and drugs in 20 genomes of Bordetella pertussis. These results suggest new possibilities for developing strategies to prevent and treat pertussis disease caused by these bacterial strains.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Immunology
Parul Kapil, Yihui Wang, Lindsey Zimmerman, Mara Gaykema, Tod J. Merkel
Summary: The resurgence of pertussis in the United States is likely due to the failure of acellular pertussis (aP) vaccines to provide long-lasting immunity and prevent infection, carriage, and transmission. By studying baboons, it was found that multiple infections were necessary to induce immune responses and protection in aP-vaccinated animals comparable to those seen in unvaccinated animals after a single challenge. Immunoglobulin G responses to vaccine and nonvaccine antigens were not negatively affected in aP-vaccinated animals. The results suggest the possibility of reprogramming aP-primed immune responses through optimal boosters and multiple doses.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Editorial Material
Microbiology
Nicholas Carbonetti
Summary: This study used a mouse model and cellular studies to investigate the bacterial and host factors that contribute to cough production during Bordetella pertussis infection. The researchers found that bacterial factors pertussis toxin, lipooligosaccharide, and Vag8 function cooperatively to produce cough, and identified the host bradykinin and signaling pathways involved. This finding is highly significant as it enhances our understanding of the pathophysiology of severe cough and may lead to potential new therapeutic approaches for pertussis-induced cough.
Article
Multidisciplinary Sciences
Keila Belhart, Federico Sisti, Monica C. Gestal, Julieta Fernandez
Summary: Bordetella bronchiseptica is a gram-negative bacterium that serves as a model for studying host-pathogen interactions. This study identifies the diguanylate cyclase BdcB as an important regulator of biofilm formation and motility in B. bronchiseptica. The absence of BdcB leads to increased cytotoxicity and pro-inflammatory response in macrophages, as well as upregulation of T3SS-mediated toxins. These findings highlight the role of BdcB in virulence regulation and host immune response in B. bronchiseptica.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Nicole Wolter, Cheryl Cohen, Stefano Tempia, Sibongile Walaza, Fahima Moosa, Mignon du Plessis, Meredith L. McMorrow, Florette K. Treurnicht, Orienka Hellferscee, Halima Dawood, Ebrahim Variava, Anne von Gottberg
Summary: Pertussis remains a significant cause of illness and hospitalization in South Africa, despite routine vaccination for six decades. Infants are the highest at-risk group for pertussis, while HIV-infected adults were also identified as an important group at risk of infection.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Yukihiro Hiramatsu, Takashi Nishida, Dendi Krisna Nugraha, Mayuko Osada-Oka, Daisuke Nakane, Katsumi Imada, Yasuhiko Horiguchi
Summary: A bacterial small RNA called Bpr4 was found to contribute to Bordetella pertussis infection by up-regulating the expression of a major adhesin called filamentous hemagglutinin (FHA) posttranscriptionally. Bpr4 up-regulation was triggered by interference with flagellar rotation, leading to activation of a diguanylate cyclase and subsequent up-regulation of Bpr4.
Article
Multidisciplinary Sciences
A. Launay, C. -J. Wu, A. Dulanto Chiang, J. -H. Youn, P. P. Khil, J. P. Dekker
Summary: In this study, the authors utilized whole genome sequencing to characterize the adaptive evolution mechanisms of Bordetella hinzii in a patient with interleukin-12 receptor beta 1 deficiency. Results suggest that the inactivation of DNA proofreading activity in combination with the underlying host immunodeficiency facilitated rapid genomic adaptation of the pathogen. This highlights the fundamental role of host immune phenotype in shaping pathogen evolution following zoonotic infection.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Karen M. Scanlon, Ling Chen, Nicholas H. Carbonetti
Summary: The study revealed that Bordetella pertussis-induced pulmonary hypertension is age-related and depends on the expression of pertussis toxin by the bacterium. Targeting pertussis toxin in treatment may help alleviate pulmonary hypertension and fatal infant infections.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Immunology
Thomas Belcher, Violaine Dubois, Alex Rivera-Millot, Camille Locht, Francoise Jacob-Dubuisson
Summary: Bordetella pertussis, the highly contagious agent of whooping cough, has evolved as a successful human pathogen by acquiring virulence factors and adapting its metabolism to the human respiratory tract. It overcomes host immune defenses through sophisticated mechanisms and interferes with vaccine-induced adaptive immunity. Ongoing evolutionary adaptation has made B. pertussis a highly successful human pathogen.
Article
Infectious Diseases
Matt Edmunds, Rachel Mearkle, Jennifer Folliard, Charlotte Anderson, Sooria Balasegaram, Nastassya Chandra, Clare Sawyer, Norman K. Fry, Sonia Ribeiro, Gemma Palmer, Michael Morgan, Gill Underhill, Nusreen Ahmad, Simon Friar, Andre Charlett, David Litt, Colin S. Brown, Gayatri Amirthalingam
Summary: The study found that school year and residential dormitory were risk factors associated with pertussis infection in a pertussis outbreak in a female secondary school in southern England. In addition, individuals assumed to have received acellular vaccines for their primary course were 1.7 times more likely to have pertussis compared to those assumed to have received whole-cell vaccines, though this difference was not statistically significant.
