Article
Biochemistry & Molecular Biology
Judith Matavacas, Deepak Anand, Claes von Wachenfeldt
Summary: Spx is a global transcriptional regulator that controls the Bacillus subtilis response to disulfide stress. The protein YjbH plays a critical role in regulating the cellular Spx levels by promoting its degradation through ClpXP-mediated degradation. Under stress conditions, YjbH forms aggregates by unknown mechanisms, leading to increased Spx levels. This study investigates the individual cell responses to disulfide stress through the Spx-YjbH system and reveals the heterogeneity in aggregate load and its implications for cellular fitness.
MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Luyi Cheng, Elise N. White, Naomi L. Brandt, Angela M. Yu, Alan A. Chen, Julius B. Lucks
Summary: The study investigates the structure and function of the Bacillus subtilis yxjA purine riboswitch and suggests a potential mechanism whereby ligand binding induces conformational changes in RNA structures. The results provide insight into how RNA functions in gene regulation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Huiru Fu, Yongjing Li, Jing Tian, Ben Yang, Yin Li, Qi Li, Shikai Liu
Summary: Rising ocean temperature poses a significant threat to marine organisms' adaptation and survival, leading to ecological impacts and economic losses. This study revealed a new regulatory mechanism mediated by hypoxia-inducible factor-1a (HIF-1a) in Pacific oysters (Crassostrea gigas) in response to heat stress. HIF-1a and HIF-1a-like5 were found to be highly induced under heat stress and played critical roles in the heat shock response (HSR) by upregulating the expression of heat shock proteins (HSP). Knocking down of HIF-1a inhibited the expression of heat shock factor 1 (HSF1) and HSP70 genes, leading to decreased survival of C. gigas under heat stress. The involvement of HIF-1a/HSF1/HSP70 pathway in the oyster's response to heat stress provides insights into the adaptive mechanisms of bivalves to ocean warming.
MARINE BIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Agnes Jakab, Fruzsina Kovacs, Noemi Balla, Zoltan Toth, Agota Ragyak, Zsofi Sajtos, Kinga Csillag, Csaba Nagy-Koteles, Daniel Nemes, Ildiko Bacskay, Istvan Pocsi, Laszlo Majoros, Akos T. Kovacs, Renato Kovacs
Summary: This study demonstrates that surfactin, a secondary metabolite derived from Bacillus subtilis, exhibits antifungal properties against Candida albicans. It inhibits the growth, adhesion, and morphogenesis of C. albicans, increases the level of reduced glutathione, and enhances the efficacy of fluconazole. Surfactin also affects the gene transcription of C. albicans, with down-regulation of genes involved in morphogenesis and metabolism, and up-regulation of genes associated with ribosome biogenesis, iron metabolism, and drug transport.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Gastroenterology & Hepatology
Helit Cohen, Boaz Adani, Emiliano Cohen, Bar Piscon, Shalhevet Azriel, Prerak Desai, Heike Bahre, Michael McClelland, Galia Rahav, Ohad Gal-Mor
Summary: This study investigates the changes in the DksA regulon during Salmonella evolution, showing that DksA plays a crucial role in the growth, gene expression, and invasion of Salmonella. It regulates multiple metabolic pathways and virulence genes. The study also reveals the assimilation of horizontally acquired virulence genes under the DksA regulon, providing insights into the evolution of virulence gene regulation in Salmonella species.
Article
Microbiology
Megan M. Dubay, Nikki Johnston, Mark Wronkiewicz, Jake Lee, Christian A. Lindensmith, Jay L. Nadeau
Summary: This study presents a system for investigating bacterial motility at high temperatures using a submerged digital holographic microscope. The researchers observed active motility in Bacillus subtilis up to 66 degrees C, with most cells becoming immobilized as temperatures rose. A fraction of cells remained highly motile at distances above the surface. The study also introduced an automated tracking package for quantifying motility parameters.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Jian Wang, Muhammad Ishfaq, Yusong Miao, Zhongyuan Liu, Mengting Hao, Changyu Wang, Jiaqi Wang, Xueping Chen
Summary: The present study evaluated the probiotic properties of Bacillus subtilis KC1 as a feed additive in poultry feed. The supplementation of Bacillus subtilis improved growth performance, heat-stress tolerance, resistance to Mycoplasma gallisepticum and Salmonella Pullorum challenge, as well as liver function and immune response of broilers challenged with Aflatoxin B1. These results suggest a tremendous potential of Bacillus subtilis KC1 as a feed additive in poultry feed.
Article
Microbiology
Patrick Fasshauer, Tobias Busche, Joern Kalinowski, Ulrike Maeder, Anja Poehlein, Rolf Daniel, Joerg Stuelke
Summary: Many bacteria encode cold shock proteins, which play important roles in bacterial growth and gene expression. In Bacillus subtilis, CspB and CspD are the most abundant cold shock proteins, and their simultaneous loss results in severe growth defects and loss of genetic competence. Overexpression of CspC can compensate for the lack of CspB and CspD. Transcription analysis shows that the absence of CspB and CspD affects the expression of about 20% of all genes and may be involved in the control of transcription termination.
Review
Toxicology
Benjamin J. Lang, Martin E. Guerrero, Thomas L. Prince, Yuka Okusha, Cristina Bonorino, Stuart K. Calderwood
Summary: Cells respond to protein-damaging stress by activating the Heat Shock Response (HSR), which plays a crucial role in determining cell survival or death. The modulation of HSR and its network have become attractive treatment strategies for neurodegenerative diseases and cancers. A detailed understanding of the mechanisms of HSR is necessary for the development of effective therapies, as important features of HSR continue to be uncovered.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Can Huang, Wenqian Li, Jingyu Chen
Summary: This study aims to investigate the function of DksA in fatty acid metabolism and cell membrane structure in Yersinia enterocolitica. The results showed that deletion of DksA resulted in decreased expression of fatty acid degradation genes and slower growth at low temperatures. Furthermore, DksA positively regulated the integrity of the inner and outer membranes of Y. enterocolitica under polymyxin B exposure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ecology
Franz-Sebastian Krah, Jaqueline Hess, Florian Hennicke, Ritwika Kar, Claus Baessler
Summary: Climate change leads to increased tree mortality and sun exposure on forest floors, potentially affecting the maturation of forest fungi mushrooms. Exposure to enhanced sun exposure triggers mushroom-specific molecular responses, including the expression of heat shock proteins. These responses may help mushrooms maintain fitness under climate change.
ECOLOGY AND EVOLUTION
(2021)
Article
Environmental Sciences
Zhenyu Hang, Peipei Tong, Pian Zhao, Zhangwei He, Linjun Shao, Yanru Jia, Xiaochang C. Wang, Zhihua Li
Summary: In this study, the stringent response of activated sludge systems to stressed conditions was examined using copper ion as a stress model. The results showed that weak stress led to an increase in AHLs concentrations and a decrease in RSG intensity. Increased stress resulted in an increase in bacteria concentration in the supernatant and a decrease in respiration rates and ATP levels. Lethal stress caused a decline in respiration rates, but an increase in Rq/t. Based on these findings, a hierarchical stringent response model was proposed.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Plant Sciences
Neeta Lohani, Mohan B. Singh, Prem L. Bhalla
Summary: Increases in extreme temperature events caused by climate change negatively affect agricultural productivity and global food security. New research has found that in high temperatures, oilseed rape anthers undergo rapid transcriptional reprogramming to respond to the unfolded protein response. Additionally, the study found that heat stress can activate the cytosolic heat response network. These findings shed light on the early stress-responsive mechanisms in plant cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Zhiwei Tu, Peter Setlow, Stanley Brul, Gertjan Kramer
Summary: Bacterial endospores, particularly those of Bacillus subtilis A163, exhibit high resistance to wet heat compared to laboratory strains, with proteomic analysis revealing differences in protein expression between the two strains that may contribute to this resistance. The absence of spore morphogenic protein SpoVM in B. subtilis A163, as well as the identification of proteins responsible for high heat resistance, provides insight into the mechanisms behind the resilience of A163 spores.
Article
Biotechnology & Applied Microbiology
Aya Sato, Misaki Takamatsu, Satona Kobayashi, Michio Ogawa, Yuh Shiwa, Satoru Watanabe, Taku Chibazakura, Hirofumi Yoshikawa
Summary: Among SigA-dependent promoters in Bacillus subtilis, the heat shock response was found to be dependent on the initiation nucleotide (iNTP) of transcription. In vivo and in vitro experiments showed that GTP led to a full response, ATP led to a reduced response, while UTP and CTP showed no additional expression. The overall intensification of transcription at high temperatures was attributed to the high efficiency of open complex formation and the high affinity of RNA polymerase (RNAP) for the initiation nucleotide GTP. Similar mechanisms were also observed in Escherichia coli RNAP.
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Anne Diehl, Yvette Roske, Linda Ball, Anup Chowdhury, Matthias Hiller, Noel Moliere, Regina Kramer, Daniel Stoeppler, Catherine L. Worth, Brigitte Schlegel, Martina Leidert, Nils Cremer, Natalja Erdmann, Daniel Lopez, Heike Stephanowitz, Eberhard Krause, Barth-Jan van Rossum, Peter Schmieder, Udo Heinemann, Kursad Turgay, Umit Akbey, Hartmut Oschkinat
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Regine Hengge, Susanne Haeussler, Mihaela Pruteanu, Joerg Stuelke, Natalia Tschowri, Kursad Turgay
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Ingo Hantke, Heinrich Schaefer, Armgard Janczikowski, Kuersad Turgay
MOLECULAR MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Heinrich Schaefer, Anja Heinz, Petra Sudzinova, Michelle Voss, Ingo Hantke, Libor Krasny, Kuersad Turgay
MOLECULAR MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Nancy Mulvenna, Ingo Hantke, Lynn Burchell, Sophie Nicod, David Bell, Kuersad Turgay, Sivaramesh Wigneshweraraj
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Genetics & Heredity
Heinrich Schaefer, Bertrand Beckert, Christian K. Frese, Wieland Steinchen, Aaron M. Nuss, Michael Beckstette, Ingo Hantke, Kristina Driller, Petra Sudzinova, Libor Krasny, Volkhard Kaever, Petra Dersch, Gert Bange, Daniel N. Wilson, Kuersad Turgay
Editorial Material
Biochemistry & Molecular Biology
David A. Dougan, Regina Alver, Kuersad Turgay
Article
Cell Biology
Patrick Pausch, Maha Abdelshahid, Wieland Steinchen, Heinrich Schaefer, Fabio Lino Gratani, Sven-Andreas Freibert, Christiane Wolz, Kuersad Turgay, Daniel N. Wilson, Gert Bange
Article
Multidisciplinary Sciences
Yvette Roske, Florian Lindemann, Anne Diehl, Nils Cremer, Victoria A. Higman, Brigitte Schlegel, Martina Leidert, Kristina Driller, Kursad Turgay, Peter Schmieder, Udo Heinemann, Hartmut Oschkinat
Summary: Studying bacterial biofilm generation is important for understanding cell-cell communication, cohabitation principles, and antibiotic resistance. Bacillus subtilis biofilms are a model system with potential applications, and their major matrix proteins are TasA and TapA. This study presents the structure of TapA and reveals its role in promoting the growth of nonamyloidic TasA filaments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Bertrand Beckert, Maha Abdelshahid, Heinrich Schaefer, Wieland Steinchen, Stefan Arenz, Otto Berninghausen, Roland Beckmann, Gert Bange, Kursad Turgay, Daniel N. Wilson
