Journal
AMERICAN JOURNAL OF PHYSICS
Volume 84, Issue 3, Pages 202-210Publisher
AMER ASSOC PHYSICS TEACHERS
DOI: 10.1119/1.4939516
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Funding
- ENS Lyon
- Agence Nationale de la Recherche francaise [ANR-11-PDOC-027]
- Region Rhone Alpes
- Programme d'Avenir Lyon - Saint Etienne (PALSE NoGELPo)
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We have developed a lab module for undergraduate students, which involves the process of quantifying the dynamics of a suspension of microscopic particles using Differential Dynamic Microscopy (DDM). DDM is a relatively new technique that constitutes an alternative method to more classical techniques such as dynamic light scattering (DLS) or video particle tracking (VPT). The technique consists of imaging a particle dispersion with a standard light microscope and a camera and analyzing the images using a digital Fourier transform to obtain the intermediate scattering function, an autocorrelation function that characterizes the dynamics of the dispersion. We first illustrate DDM in the textbook case of colloids under Brownian motion, where we measure the diffusion coefficient. Then we show that DDM is a pertinent tool to characterize biological systems such as motile bacteria. (C) 2016 American Association of Physics Teachers.
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