Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 174, Issue -, Pages 409-415Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2018.11.048
Keywords
Murine vulvovaginal candidiasis; Miconazole; Chitosan-based nanoparticles
Funding
- Fundacao de Apoio a Pesquisa do Distrito Federal (FAPDF) [193.000328/2007]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [559157/2008-8]
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Objectives: In this study, polymeric nanoparticles based on chitosan incorporating the antifungal miconazole nitrate were fabricated and testedin vivo using murine vulvovaginal candidiasis. Methods: Nanoparticles prepared by the ionotropic gelation method presented 200 to 300 nm diameter and polydispersity indexes ranging from 0.2 to 0.4. The nanoparticles were prepared to incorporate 63.9 mg/mL of miconazole nitrate to be testedin vivo. Murine vulvovaginal candidiasis was standardized using estradiol valerate before the animals were challenged by Candida albicans. Results: The treatment using chitosan nanoparticles within miconazole nitrate presented the same therapeutic efficacy as miconazole nitrate in a commercial cream formulation, however using the antifungal content about seven-fold lower. This increase in the miconazole nitrate's therapeutic efficacy is may be due to the down-regulation of interleukin 10 (IL-10) expression. Conclusions: Our data represent a proof of concept that can be exploited to achieve an alternative and promising therapy for the treatment of vulvovaginal candidiasis.
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