Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, General & Internal
Rintaro Saito, Masahiro Sugimoto, Akiyoshi Hirayama, Tomoyoshi Soga, Masaru Tomita, Toru Takebayashi
Summary: Large-scale metabolomic studies are common, but there is less focus on the reliability of uncharacterized peaks in untargeted metabolomics data. This study tested criteria to assess the reliability of uncharacterized peaks and identified some reliable peaks potentially related to important physiological processes like disease progression.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Food Science & Technology
Maria Castro-Puyana, Maria L. Marina
Summary: This review article critically examines the significant advances in using CE-MS-based approaches to assess various aspects of Food Science, and discusses the latest developments in hardware and software.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hitoshi Iwamoto, Masaaki Okihara, Isao Akashi, Yu Kihara, Osamu Konno, Shigeyuki Kawachi, Makoto Sunamura, Masahiro Sugimoto
Summary: Kidney biopsy is commonly used to diagnose kidney transplant dysfunction. In this study, capillary electrophoresis-mass spectrometry was used to analyze the biofluids of transplant recipients. Metabolomics analyses revealed significant differences in the metabolomic profiles of the different groups, particularly in arginine biosynthesis and purine metabolism in plasma samples. Different types of kidney disorders also showed distinct metabolomic profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Marlien van Mever, Magdalena Fabjanowicz, Maricruz Mamani-Huanca, Angeles Lopez-Gonzalvez, Justyna Plotka-Wasylka, Rawi Ramautar
Summary: The composition of wine is influenced by environmental factors, genetic factors, and winemaking practices. Metabolomics using NMR spectroscopy, GC-MS, and/or LC-MS is a useful approach for assessing the origin, authenticity, and quality of wines. This study demonstrates that CE-MS is a suitable method for analyzing polar ionogenic metabolites in wine.
Review
Chemistry, Analytical
Tomoyoshi Soga
Summary: Capillary electrophoresis mass spectrometry (CE-MS) is a powerful tool for comprehensive analysis of low-molecular-weight charged metabolites. This review discusses the strategies and principles of CE-MS-based metabolomics analyses, including sample preparation, CE separation modes, interfacing techniques, and mass spectrometers. Additionally, it highlights new techniques to increase throughput ability in CE-MS for metabolomics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, General & Internal
Wakako Yatsuoka, Takao Ueno, Kanako Miyano, Ayame Enomoto, Sana Ota, Masahiro Sugimoto, Yasuhito Uezono
Summary: This study analyzed the time-course of salivary metabolomic profiles in patients with head and neck cancer undergoing radiation therapy. Specific metabolites such as histidine and tyrosine were found to be associated with the severity of oral mucositis before the start of radiation therapy. Further research is needed to validate these findings.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Kaori Igarashi, Sana Ota, Miku Kaneko, Akiyoshi Hirayama, Masanobu Enomoto, Kenji Katumata, Masahiro Sugimoto, Tomoyoshi Soga
Summary: A high-throughput polyamine metabolite profiling method based on multisegment injection capillary electrophoresis triple quadrupole tandem mass spectrometry was developed in this study. The method is sensitive, selective, and quantitative, allowing for rapid screening of polyamines in a large number of samples and discrimination of disease patients.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Zi-Ao Huang, Jiahua Tan, Yueyang Li, Siyu Miao, Kymora B. Scotland, Ben H. Chew, Dirk Lange, David D. Y. Chen
Summary: Migration time correction is crucial in CE-based metabolomics, especially for dual pressure CE. We proposed a new correction method and developed a script tool for migration time correction and peak alignment. A case study demonstrated the effectiveness of this method.
Article
Chemistry, Analytical
Qinlei Liu, Sandra Martinez-Jarquin, Wenjie Ge, Renato Zenobi
Summary: Understanding diseases requires understanding metabolic heterogeneity in cells. This technical note introduces a 3D printing technology for manufacturing an ionization source for mass spectrometry-based single-cell analysis. The all in-one 3D-printed electrospray ionization source simplifies the process and improves reproducibility. It has been successfully used for high-throughput analysis of cancer cells and can be adjusted for different cell sizes. This 3D-printed ionization source opens new possibilities for single-cell analysis.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Susanna Della Posta, Chiara Fanali, Valeria Gallo, Salvatore Fanali
Summary: This paper presents recent results obtained by coupling electromigration techniques with mass spectrometry, highlighting the advantages of this hybrid approach and discussing selected applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Bingshu He, Wei Zhang, Faisa Guled, Amy Harms, Rawi Ramautar, Thomas Hankemeier
Summary: This paper discusses the crucial factors for the performance of biomass-restricted metabolomics studies, including sampling and sample preparation methods, separation techniques, and ionization sources. It also provides overviews of MS-based miniaturized metabolomics studies reported over the past five years, with information on sample type, sample preparation volume, injection volume, separation techniques, and MS analyzers.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Radim Polasek, Klara Konderlova, Jan Petr
Summary: The aim of this study was to develop a new method for analyzing tamsulosin enantiomers using capillary electrophoresis combined with tandem mass spectrometry. The method achieved a limit of detection of 1.6 nmol L-1 under optimal conditions. It was validated for linearity, detection and quantification limits, precision, recovery, and selectivity, and can be applied to the analysis of tamsulosin enantiomers in environmental samples and other analytical tasks.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Bo Liu, Wentao Wang, Tie Gao, Lu Huang, Huihong Fan, Hong-Xu Chen
Summary: Cobratide, a peptide drug extracted from Chinese cobra venom, has shown potential in treating not only chronic pain but also COVID-19. A protocol combining sheathless CE-MS and CE-UV methods was established for the separation, identification, and quantification of cobratide and its impurities, providing valuable information for quality control and process analysis of peptide drugs.
ANALYTICAL METHODS
(2021)
Article
Pediatrics
Kazuko Eto, Masahiro Sugimoto
Summary: The study found that a higher proportion of parents of elementary school students had experiences of initiating alcohol use in their children compared to parents of kindergarten children. However, there was no significant difference in parents' knowledge regarding the effects of alcohol on youth between the two parent groups. These data indicate that the age of children is the only factor as opposed to parents' knowledge.
Article
Cell Biology
Chen Chen, Yoshiaki Yamanaka, Koji Ueda, Peiying Li, Tamami Miyagi, Yuichiro Harada, Sayaka Tezuka, Satoshi Narumi, Masahiro Sugimoto, Masahiko Kuroda, Yuhei Hayamizu, Kohsuke Kanekura
Summary: The pathogenicity of R-rich DPRs is primarily mediated by disrupting and trapping proteins through LLPS, which is controlled by the distribution of charged Arg residues.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Medicine, General & Internal
Wakako Yatsuoka, Takao Ueno, Kanako Miyano, Ayame Enomoto, Sana Ota, Masahiro Sugimoto, Yasuhito Uezono
Summary: This study analyzed the time-course of salivary metabolomic profiles in patients with head and neck cancer undergoing radiation therapy. Specific metabolites such as histidine and tyrosine were found to be associated with the severity of oral mucositis before the start of radiation therapy. Further research is needed to validate these findings.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Serafettin Gunes, Yumi Aizawa, Takuma Sugashi, Masahiro Sugimoto, Pedro Pereira Rodrigues
Summary: Early diagnosis is crucial for delaying the onset of Alzheimer's disease, and current diagnostic methods are invasive and expensive, highlighting the need for more convenient and effective biomarkers. Studies have identified potential biomarkers, but further validation research is necessary.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yoji Saeki, Akane Takenouchi, Etsuyo Otani, Minji Kim, Yumi Aizawa, Yasuko Aita, Atsumi Tomita, Masahiro Sugimoto, Takashi Matsukubo
Summary: This study evaluated the effect of long-term mastication on salivary metabolomic profiles and found that most metabolites showed lower concentrations after chewing. The pathways related to glycogenic amino acids also underwent significant changes.
Article
Gastroenterology & Hepatology
Taisuke Matsumoto, Masakatsu Fukuzawa, Takao Itoi, Masahiro Sugimoto, Yumi Aizawa, Makoto Sunamura, Takashi Kawai, Daiki Nemoto, Hirokazu Shinohara, Takahiro Muramatsu, Yuka Suzuki, Yasuyuki Kagawa, Maya Suguro, Kumiko Uchida, Yohei Koyama, Akira Madarame, Takashi Morise, Hayato Yamaguchi, Akihiko Sugimoto, Yoshiya Yamauchi, Shin Kono, Sakiko Naito
Summary: This study analyzed plasma metabolomics and identified a combination of quantified metabolites that showed high sensitivity for the detection of gastric cancer (GC). These metabolites were found to be independent of commonly used tumor markers, suggesting that their complementary use could be helpful for GC screening.
Article
Biochemistry & Molecular Biology
Hitoshi Iwamoto, Masaaki Okihara, Isao Akashi, Yu Kihara, Osamu Konno, Shigeyuki Kawachi, Makoto Sunamura, Masahiro Sugimoto
Summary: Kidney biopsy is commonly used to diagnose kidney transplant dysfunction. In this study, capillary electrophoresis-mass spectrometry was used to analyze the biofluids of transplant recipients. Metabolomics analyses revealed significant differences in the metabolomic profiles of the different groups, particularly in arginine biosynthesis and purine metabolism in plasma samples. Different types of kidney disorders also showed distinct metabolomic profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daisuke Nose, Masahiro Sugimoto, Tsuneo Muta, Shin-Ichiro Miura
Summary: Pancreatic cancer is a highly malignant cancer with a poor prognosis. It is often diagnosed late due to the absence of typical symptoms. Therefore, it is necessary to establish a screening method for early detection of pancreatic cancer in high-risk individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Yoshie Nakayama, Mariko Hanada, Hiroshi Koda, Masahiro Sugimoto, Masahiro Takada, Masakazu Toi
Summary: This study aimed to evaluate volatile compounds in exhaled breath as a non-invasive screening method to detect breast neoplasms. After analyzing breath samples, significant differences in volatile compounds were found between patients with breast cancer and non-breast cancer controls. The developed MLR model can effectively discriminate between breast cancer and non-breast cancer samples. This study suggests the potential of volatile compounds in exhaled breath as a noninvasive screening method for breast cancer.
JOURNAL OF BREATH RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Akiyoshi Hirayama, Takamasa Ishikawa, Haruka Takahashi, Sanae Yamanaka, Satsuki Ikeda, Aya Hirata, Sei Harada, Masahiro Sugimoto, Tomoyoshi Soga, Masaru Tomita, Toru Takebayashi
Summary: High-throughput metabolomics has facilitated large-scale cohort studies. Long-term studies require multiple batch-based measurements, which necessitates sophisticated quality control to eliminate bias. In this study, liquid chromatography-mass spectrometry was used to analyze 10,833 samples in 279 batch measurements. The quantified profile included 147 lipids. Normalization using QC samples reduced the coefficient of variation for the lipids. This study provides unbiased and quantified data for large-scale metabolomics.
Article
Oncology
Sachika Shiraishi, Masahiro Sugimoto, Koichi Tokuuye
Summary: The study evaluated salivary metabolomics as an independent predictor for radiation pneumonitis, identifying three metabolites with predictive ability for clinical RP.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Chemistry, Medicinal
Hiroshi Arakawa, Takumi Kawanishi, Dai Shengyu, Takumi Nishiuchi, Makiko Meguro-Horike, Shin-ichi Horike, Masahiro Sugimoto, Yukio Kato
Summary: This study investigates the effects of hepatobiliary cholestasis on renal exposure to nephrotoxic drugs. The results show that tissue accumulation of imatinib and cisplatin in kidney slices is higher in mice with bile duct ligation (BDL) compared to sham-operated mice. Proteomic analysis reveals a reduction in the expression of the efflux transporter Mrp6 in the kidneys of BDL mice. These findings suggest that cholestasis leads to drug accumulation in renal cells and promotes drug-induced renal injury.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Daisuke Nose, Tomokazu Matsui, Takuya Otsuka, Yuki Matsuda, Tadaaki Arimura, Keiichi Yasumoto, Masahiro Sugimoto, Shin-Ichiro Miura
Summary: A study developed a model to estimate pleural effusion in heart failure patients using multi-frequency bioelectrical impedance analysis, which showed high accuracy in discriminating patients with pleural effusion. This approach offers a potential clinical application for assessing intrathoracic conditions.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Health Care Sciences & Services
Masahiro Sugimoto, Atsumi Tomita, Michiko Oyamada, Mitsue Sato
Summary: The gaze trajectories of nurses with different levels of experience were monitored during an intravenous injection task. The results showed that experienced nurses had different patterns of gaze duration compared to nursing students. A state transition diagram visualized the recognition errors and repeated confirmation behaviors. Clustering analysis of gaze durations also indicated similarity among participants with similar experiences.
Article
Multidisciplinary Sciences
Misa Yoshizawa, Masahiro Sugimoto, Minoru Tanaka, Yusuyuki Sakai, Masaki Nishikawa
Summary: Liver fibrosis is a result of homeostasis breakdown caused by repetitive injury, leading to collagen accumulation that disrupts liver structure and function, resulting in serious consequences such as cirrhosis. Study using mathematical simulation model found accumulation of collagens from different sources in different regions of the liver, providing insights into a new mechanism for understanding liver fibrosis.
SCIENTIFIC REPORTS
(2022)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)
