Review
Agriculture, Multidisciplinary
Cong Hu, Yuan Zhang, Yu Zhou, Yang-jia-yi Xiang, Zhi-fei Liu, Zhi-hong Wang, Xue-song Feng
Summary: This review summarizes the recent advances in the detection methods of Tetrodotoxin (TTX) and its analogues. The focus of current research is on sample preparation and analytical techniques, with the introduction of several state-of-the-art technologies. The review provides valuable insights for the future development of TTX detection methods.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Catherine L. J. Brown, Hannah Scott, Crystal Mulik, Amy S. Freund, Michael P. Opyr, Gerlinde A. S. Metz, G. Douglas Inglis, Tony Montina
Summary: Analysis of fecal metabolome can provide valuable diagnostic information about enteric microbiota function. However, the presence of macromolecules in fecal samples hinders accurate analysis. This study tested three methods and found that ultrafiltration effectively removes macromolecules, enabling accurate analysis.
Review
Chemistry, Analytical
Adriana Leandra Santoro, Pedro H. Vendramini, Deisi L. Braga, Lucas Gelain Martins, Gabriel F. Dos Santos, Gesiane S. Lima, Ljubica Tasic, Marcelo A. Mori
Summary: This review presents the main steps involved in C. elegans lipidomics and metabolomics analyses, discussing the advantages and drawbacks of different methods and listing commonly used protocols and applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Cell Biology
Christoph Seger, Sonja Sturm
Summary: NMR spectroscopy is essential for determining the structural information of analytes from natural sources in liquid state. While mass spectrometry can differentiate and group biomarkers, the structural characterization often requires the use of NMR spectroscopy.
Article
Chemistry, Analytical
Miso Nam, Sae Rom Jo, Jae Ho Park, Min-Sun Kim
Summary: This study examined the effects of solvent, collection method, and storage stability on salivary metabolic profiles. The results showed that acetonitrile was more effective at removing large molecules from saliva samples compared to methanol. The levels of 15 metabolites varied between unstimulated and stimulated saliva. Storage at room temperature for 24 hours increased amino acid levels and decreased other metabolites. Overall, this study provides insights into the diversity, stability, and potential disease biomarkers of salivary metabolic profiles.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Applied
Aghiles Karim Aissat, Nassima Chaher-Bazizi, Tristan Richard, Dina Kilani-Atmani, Eric Pedrot, Elodie Renouf, Djebbar Atmani, Josep Valls Fonayet
Summary: This study investigated the phenolic composition of Pistacia lentiscus L. fruits at different physiological stages. The results revealed significant variation in the qualitative and quantitative content of phenolic compounds during fruit ripening, suggesting potential applications as nutraceuticals and food colorants.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Analytical
Marta Roca, Maria Isabel Alcoriza, Juan Carlos Garcia-Canaveras, Agustin Lahoz
Summary: Metabolomics is a valuable tool for studying metabolism and biomarker discovery. Liquid chromatography-mass spectrometry (LC-MS) is widely used at high resolution, and analysis of open metabolite databases helps estimate the size of the detected human metabolome to guide LC-MS analysis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Liliia Usoltseva, Vitaliy Ioutsi, Yuriy Panov, Mariya Antsupova, Liudmila Rozhinskaya, Galina Melnichenko, Natalia Mokrysheva
Summary: This study describes the development of an HPLC-DMS-MS/MS method for the quantitation of vitamin D compounds in serum. The method combines liquid-liquid and solid-phase extraction for sample preparation, eliminating the need for a lengthy derivatization step. Sensitivity is improved through the implementation of differential ion mobility separation. The assay shows good linearity, wide analytical range, and acceptable precision for all metabolites. The detection limit for 1,25(OH)(2)D-3 is 10 pg/mL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Liene Bervoets, Johannes H. Ippel, Agnieszka Smolinska, Niels van Best, Paul H. M. Savelkoul, Monique A. H. Mommers, John Penders
Summary: Efforts to optimize and standardize fecal metabolomics in adults have been successful, but a standard protocol for infant feces analysis is still lacking. A hands-on protocol for proton H-1 NMR spectroscopy of infant feces was developed, recommending water extraction with a dilution ratio of 1:5, bead beating for homogenization, and centrifugation for particulate removal. Analysis showed varied biochemical compositions in infant feces from different age groups.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Biochemical Research Methods
Israel Donizeti de Souza, Maria Eugenia Costa Queiroz
Summary: Alzheimer's disease (AD), characterized by memory loss, confusion, and cognitive impairment, is a major public health concern. Researchers have focused on finding reliable biomarkers for early diagnosis, with amyloid-beta (A beta) peptides being established as important indicators. However, quantifying A beta peptides in biological samples remains challenging due to their complex properties. Mass spectrometry techniques, such as HPLC-MS/MS, have shown promise in accurately measuring A beta peptides and have led to advancements in sample preparation and sensitivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Maxim Fomenko, Lyudmila Yanshole, Yuri P. Tsentalovich
Summary: Thermal and enzymatic reactions can significantly alter tissue metabolomic content during sample preparation. Stability of metabolites in human blood, serum, rat brain, and their extracts was evaluated. The results demonstrated that metabolites in tissue extracts remained stable for 24 hours at 4 degrees C. Serum and whole blood metabolomes were also relatively stable, with noticeable changes in whole blood only occurring after 1-2 hours at 4 degrees C, and becoming more significant after 24 hours. The most significant changes were observed in energy metabolites, with a decrease in ATP and ADP concentrations and below detectable levels for NAD, NADH, and NADPH. AMP, IMP, hypoxanthine, and nicotinamide showed a statistically significant increase. Brain tissue exhibited more rapid metabolomic changes during harvest and homogenization, with noticeable changes in all compound classes observed over longer time periods.
Article
Fisheries
Hua Yang, Xueni Qiu, Yun Zhao, Yangfang Ye, Weiwei Song, Changkao Mu, Ronghua Li, Chunlin Wang
Summary: This study investigated the optimal anesthetic concentration for cuttlefish using NMR and HPLC analysis. They found that ethanol at 10-30 mL L-1 successfully anesthetized cuttlefish, with recovery observed after 60 minutes at 10 mL L-1 concentration. Cuttlefish also reached a sedative state below 5 mL L-1 ethanol, with a maximum exposure time of 270 minutes. The action of ethanol led to changes in neurotransmitter levels, with increased inhibitory neurotransmitters and decreased excitatory neurotransmitters observed.
Article
Environmental Sciences
Nikita Bakanov, Carolina Honert, Lisa Eichler, Gerlind U. C. Lehmann, Ralf Schulz, Carsten A. Bruehl
Summary: A simple acetonitrile-based extraction method using HPLC-ESI-MS/MS was developed for the determination of 98 current-use pesticides in soil and herbaceous vegetation. The method exhibited good accuracy and precision, with percentage recoveries ranging from 71-125% (soil) and 70-117% (vegetation), and relative standard deviations of 1-14% (soil) and 1-13% (vegetation). Matrix-matched calibration curves showed excellent linearity (R-2 > 0.99). The method was successfully applied to agricultural sites in Germany, where 44 of the 98 pesticides were detected, indicating a high qualitative load compared to EU arable soils.
Article
Chemistry, Analytical
Dominika Kolodziej, Lukasz Sobczak, Krzysztof Gorynski
Summary: The study evaluated the use of 3D printed thermoplastic polymers as stationary phases for extracting 43 diverse analytes from human oral fluid samples. Exceptional results were observed for three polymer types: polyamide 6, LAYFOMM-60, and S-FLEX 90A. An automated 3D printing method was introduced for fast fabrication of high-throughput sample preparation devices. This method provides a safer and more convenient approach for laboratory personnel and reduces negative environmental impacts.
Review
Food Science & Technology
Abdul-Hamid M. Emwas, Nahla Al-Rifai, Kacper Szczepski, Shuruq Alsuhaymi, Saleh Rayyan, Hanan Almahasheer, Mariusz Jaremko, Lorraine Brennan, Joanna Izabela Lachowicz
Summary: The relationship between health and nutrition is closely linked, with poor nutrition potentially leading to various metabolic diseases. Metabolomics, as an emerging field of science, has broad research applications and potential for further advancement.
Article
Agriculture, Multidisciplinary
Verena Gratl, Sonja Sturm, Elena Zini, Thomas Letschka, Marco Stefanini, Silvia Vezzulli, Hermann Stuppner
Summary: The study comparing the polyphenolic profiles of hybrid grapevine varieties from international breeding programs and private initiatives in the same vineyard showed similar overall profiles but some variations in specific compounds. This research will help in selecting promising candidates for further breeding programs aimed at developing highly resistant grape varieties with improved sensory characteristics.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Plant Sciences
Sophia Mayr, Simon Strasser, Christian G. Kirchler, Florian Meischl, Stefan Stuppner, Krzysztof B. Bec, Justyna Grabska, Sonja Sturm, Hermann Stuppner, Michael A. Popp, Gunther K. Bonn, Christian W. Huck
Summary: Near-infrared spectroscopy was used to analyze the content of silymarin and individual flavonolignans in milk thistle seeds. The benchtop device NIRFlex N-500 showed the best performance, while quantum chemical simulation of spectra helped to understand the impact of chemical and physical properties on analysis. Miniaturized near-infrared spectroscopy proved to be capable of rapid and nondestructive quantification of silymarin and flavonolignans in whole milk thistle seeds.
Review
Medical Laboratory Technology
Stein Bergan, Merce Brunet, Dennis A. Hesselink, Kamisha L. Johnson-Davis, Pawel K. Kunicki, Florian Lemaitre, Pierre Marquet, Mariadelfina Molinaro, Ofelia Noceti, Smita Pattanaik, Tomasz Pawinski, Christoph Seger, Maria Shipkova, Jesse J. Swen, Teun van Gelder, Raman Venkataramanan, Eberhard Wieland, Jean-Baptiste Woillard, Tom C. Zwart, Markus J. Barten, Klemens Budde, Maja-Theresa Dieterlen, Laure Elens, Vincent Haufroid, Satohiro Masuda, Olga Millan, Tomoyuki Mizuno, Dirk J. A. R. Moes, Michael Oellerich, Nicolas Picard, Linda Salzmann, Burkhard Toenshoff, Ron H. N. van Schaik, Nils Tore Vethe, Alexander A. Vinks, Pierre Wallemacq, Anders Asberg, Loralie J. Langman
Summary: This report provides an updated and comprehensive presentation of consensus on personalized treatment with MPA, including topics such as analytical criteria, pharmacogenetics, biomarker development, and practical intervention aspects. Graded recommendations on target ranges for selected topics, such as the limited sampling strategies for MPA area under the curve, are included to aid in personalized dosing.
THERAPEUTIC DRUG MONITORING
(2021)
Article
Biochemistry & Molecular Biology
Sonja Sturm, Christina Hoegner, Christoph Seger, Hermann Stuppner
Summary: The study investigated the in vitro metabolism of vitetrifolin D using HPLC-DAD-QTOF-MS and HPLC-SPE-NMR, identifying 27 phase I and phase II metabolites, with structural characterization of nine phase I metabolites. By avoiding preparative isolation of metabolites, the study demonstrated the successful performance of in vitro metabolism studies using hyphenated instrumental analysis methodologies even with limited substance availability.
Article
Agriculture, Multidisciplinary
Nora Gibitz-Eisath, Christoph Seger, Stefan Schwaiger, Sonja Sturm, Hermann Stuppner
Summary: This study presents the first ultra-high performance supercritical fluid chromatography-diode array detector (UHPSFC-DAD) assay for simultaneous quantitation of secoiridoids, iridoids, xanthones, and xanthone glycosides in Gentiana lutea L. The method validation confirmed the assay's sensitivity, linearity, precision, and accuracy. The practical applicability was proven by the analysis of root specimens and commercial liquid preparations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Linda Salzmann, Janik Wild, Neeraj Singh, Tobias Schierscher, Franziska Liesch, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: This study developed a reference measurement procedure for gabapentin, using quantitative nuclear magnetic resonance (qNMR) spectroscopy to determine the absolute content of standard materials and isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) to accurately measure the concentration of gabapentin in serum and plasma. The analytical protocol showed good selectivity, precision, and accuracy, allowing for standardization of routine tests and reliable evaluation of clinical samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Christoph Seger, Anja Kessler, Judith Taibon
Summary: For molecules that can be well described metrologically and recorded analytically, traceability to metrologically sound foundation is necessary. National Metrology Institutes (NMIs) play a leading role in the establishment of traceability chains. If NMIs cannot take up this task, alternative approaches must be considered, such as traceability initiatives by the in vitro device industry or academia adhering to quality standards of NMIs. Adherence to ISO procedure 15193 is essential for the establishment of reference measurement procedures (RMPs), and thorough CRM characterization and JCTLM listing are crucial for both RMPs and CRMs.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Valentin Braun, Hermann Stuppner, Lorenz Risch, Christoph Seger
Summary: Steroid analysis in clinical laboratories is currently dominated by immunoassays, but liquid chromatography coupled to mass spectrometry (LC-MS/MS) has been proven to be a more capable tool with better sensitivity, specificity, and the ability to analyze multiple steroids and metabolites simultaneously, providing more reliable and comprehensive diagnostic information for endocrinologists.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Christoph Seger, Sonja Sturm
Summary: NMR spectroscopy is essential for determining the structural information of analytes from natural sources in liquid state. While mass spectrometry can differentiate and group biomarkers, the structural characterization often requires the use of NMR spectroscopy.
Article
Cell Biology
Valentin Braun, Hermann Stuppner, Christoph Seger
Summary: Screening for potential interferences in multi-analyte LC-MS/MS method development should not only cover substances of the same class but also include commonly prescribed drugs. In this study, two newly discovered potential interferences from non-steroidal drugs, paroxetine (PX) and alpha-hydroxytriazolam (alpha-OH-TZM), were identified as interferences to 17-hydroxyprogesterone (17P) and aldosterone (ALDO) respectively. These interferences should be considered clinically relevant as PX and TZM are frequently prescribed drugs with therapeutic ranges far exceeding the reference ranges of 17P or ALDO.
Article
Medical Laboratory Technology
Linda Salzmann, Tino Spescha, Neeraj Singh, Tobias Schierscher, Martina Bachmann, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: In this study, an isotope dilution (ID)-liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based candidate reference measurement procedure (RMP) was developed for the measurement of lamotrigine in human serum and plasma. The method provides traceability and reliability for standardization of routine assays and evaluation of clinical samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Anja Kobel, Tobias Schierscher, Neeraj Singh, Linda Salzmann, Franziska Liesch, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: Developed an isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure (RMP) for levetiracetam quantification in human serum and plasma. The RMP was highly selective and specific, with no evidence of a matrix effect. Its expanded measurement uncertainty of <= 4.0% meets the clinical needs in levetiracetam monitoring. Utilized quantitative nuclear magnetic resonance spectroscopy (qNMR) to ensure metrological traceability to SI units for levetiracetam reference materials.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Linda Salzmann, Tino Spescha, Neeraj Singh, Anja Kobel, Vanessa Fischer, Tobias Schierscher, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: A novel reference measurement procedure (RMP) was developed and validated for the quantification of topiramate in human serum and plasma. The method showed high analytical performance, fulfilling the requirements on measurement uncertainty, and is suitable for routine assay standardization and clinical sample evaluation.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)
